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The purpose of the study was to measure the cement thickness obtained when ceramic rods were luted to dentin and to analyze the relation between cement thickness and the previously published tensile bond strength of similar test specimens. In addition, the ISO standard 4049:2019 method was used to determine the film thickness of the used cements. Zirconia (n = 100) and lithium disilicate (n = 50) rods were cemented to bovine dentin using one of five different resin-based cements. The ceramic-dentin test specimens were cut into two slices and the cement thickness was measured using a scanning electron microscope and compared to the bond strength values of similar specimens already published. The mean cement thickness recorded for ceramic rods cemented to dentin was in the range 20-40 µm, which was larger than the cement film thickness found by the ISO method. The cement film thickness determined according to ISO standard methods did not concur with the results obtained when cementing ceramic rods to dentin. For cementing ceramic restorations, a cement thickness in the range 25-35 µm seems to be favorable for the bond strength.

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Objectives: To investigate temperature changes in various test specimens during thermal cycling and to evaluate difference in micro-tensile repair bond strength in specimens cut from the inner or the outer area of composite blocks after thermal cycling. Materials and methods: Four rectangular composite blocks of various sizes were fabricated, and thermocouples placed in the centre of the specimens or halfway from the surface to the centre. Composite cylinders were made on ground flat extracted molars, as intended for micro-tensile and shear bond testing, with a thermocouple placed at the centre of the cylinder radius between composite and dentin. The specimens were thermal cycled between 5 °C and 55 °C with 20-60 s dwell times. The highest and lowest temperatures in the test specimens were recorded. Composite blocks were fabricated and stored in water for a week and then repaired with the same composite. After thermal cycling (5000×, 5 °C and 55 °C with a 20 s dwell time), test specimens were cut for micro-tensile testing. Results: None of the specimens tested reached the cold and warm water bath temperatures after a 20 s dwell time. In the smallest composite block, the centre core temperature reached 5 °C and 55 °C after 40 s dwell time, but lacked 1 °C after 60 s in the largest block. None of the specimens involving teeth reached water temperatures. The micro-tensile repair strength was significantly different between the outer and the central cut rods (p < .05). Conclusions: The most commonly used dwell times for thermal cycling are insufficient to create a homogeneous temperature change.

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Methacrylate monomers have been identified in aqueous extracts of freshly cured dental fillings. The hypothesis tested presently was that low concentrations of triethyleneglycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA) alone or in combination interfere with the LPS-induced release of cytokines from the macrophage cell line RAW264.7. The cells were exposed to 5-200 µM of monomers for 24 h followed by a 24 h combined exposure to monomers and LPS. TEGDMA reduced LPS-induced release of interleukin-1ß (IL-1ß) and tumor necrosis factor-α (TNF-α), whereas HEMA only reduced IL-1ß release. Co-exposure to the two monomers indicated an additive effect. Moreover, the reduced cytokine release persisted for 24 h after termination of the monomer exposure. The LPS-induced activation of proteins in pre-transcriptional signaling pathways (CD14, p-ERK1/2, p-p38, p-JNK, p-IκB-α and p-NFκB-p65) was not altered by monomer exposure, neither were the levels of IL-1ß and TNF-α mRNA. However, the LPS-induced level of pro-IL-1ß was decreased by the monomer treatment. Thus, HEMA and TEGDMA may interfere with post-transcriptional regulation of synthesis and release of these cytokines. Overall, the results suggest that low concentrations of monomers may cause impaired macrophage responses, and that these effects can persist for up to 24 h after exposure.

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The purpose of this study was to assess and compare the cytotoxicity of liquid and powder components of chemically different dental materials using 2 basic unspecific cell culture methods. Three chemically cured glass ionomers (Fuji II, Lining cement, and Ketac Silver), 1 light-cured glass ionomer (Fuji II LC), and 2 chemically cured acrylates (Swedon and Super Bond) were tested. The liquids were diluted 1:10 in cell culture medium. The liquids from chemically cured acrylates were further diluted 1:100, 1:1000, and 1:10000. Extracts were made by incubating the powders in cell culture medium for 24 h at 37 degrees C according to the ISO standard 10993-12. The cytotoxicity was assessed in transformed mouse fibroblasts (L-929) using two viability assays, dimethylthiazol diphenyltetrazolium (MTT) and neutral red (NR). Severe cytotoxicity was observed when testing powder extracts of Swedon, Fuji II, and Lining cement, whereas powder extracts of Ketac Silver, Fuji LC, and Super Bond induced slight to non-cytotoxicity. All of the 1:10 liquid dilutions were severely cytotoxic in the MTT assay. In the NR assay, however, four 10% dilutions were severely cytotoxic and 4 moderately cytotoxic. Further dilution of the liquids of the chemically cured acrylates reduced the toxicity, while the Super Bond catalyst was severely cytotoxic even as the 1:100 dilutions.

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