RESUMEN
A practical and economical capillary ion electrophoresis method with indirect UV detection at 214 nm was developed for determination of inorganic cations in plants of Ilex paraguariensis(St. H.) and their infusion known as mate tea, a very popular beverage in South America. A microwave digestion procedure was used to prepare the herbal plants, but the infusion was only diluted. The background electrolyte contained 6mM imidazole and 10mM alpha-hydroxyisobutyric acid, pH 4.0. The running voltage was 20 kV and temperature was 25 degrees C. K, Na, Ca, Mg, and Mn ions were quantitated, and linearity was demonstrated between 0.6 and 120 ppm. The results were in good agreement with those obtained by flame atomic absorption and emission spectrometry. Accuracy of the method was verified by comparison with Beech leaves CRM 100, a standard reference material. The high content of minerals and several oligoelements, especially Mn in mate tea, is considered to be of nutritional interest.
Asunto(s)
Bebidas/análisis , Cationes/análisis , Plantas/química , Electroforesis Capilar , Indicadores y Reactivos , Microondas , Hojas de la Planta/química , Estándares de Referencia , Reproducibilidad de los Resultados , Soluciones , América del Sur , Espectrofotometría AtómicaRESUMEN
A simple and reproducible micellar electrokinetic chromatography (MEKC) method has been developed for the quantitation of suramin serum levels to be used for its therapeutic drug monitoring (TDM). A running buffer solution of 20 mM sodium borate, 75 mM sodium dodecyl sulfate (SDS), and 4 M urea at pH 9.2 were employed and samples were introduced directly into the capillary. The voltage applied for sample separation was 25 kV and UV detection was at 254 nm. Linearity was proved over the range 47.6 micrograms/mL-523.6 micrograms/mL of suramin (r: 0.9996). Orange G was used as internal standard and no interferences of common drugs simultaneously administered to patients were observed. Recovery values of the intraday and interday assays were between 92.5 and 97.2%.