RESUMEN
Growth of fastidious adenovirus serotype 40 (Ad40) in several cell lines was investigated. Ad40 was able to readily propagate in human intestinal cell line, HRT 18. Coinfection assays were made in non-permissive and permissive cells between Ad40 and Ad5dl312 or dl1520, mutants deleted in E1A and E1B regions, respectively, to test the ability of Ad40 to complement these mutants and vice versa. Ad40 could enhance Ad5dl312 DNA synthesis in HRT18 and HeLa cells, although its own DNA disappeared in the presence of this mutant in HRT18 cells. In coinfection with dl1520, Ad40 DNA synthesis was inhibited by dl1520 in HRT18 cells and dl1520 DNA synthesis was inhibited by Ad40 in 293 cells. This might reflect the presence of unusual products encoded by Ad40 E1B region.
Asunto(s)
Adenovirus Humanos/crecimiento & desarrollo , Genes Virales/genética , Proteínas Oncogénicas Virales/genética , Proteínas Precoces de Adenovirus , Replicación del ADN , ADN de Cadena Simple/biosíntesis , Prueba de Complementación Genética , Humanos , Mutación , Reacción en Cadena de la Polimerasa , Serotipificación , Replicación ViralRESUMEN
Plasmids containing the E1 regions of adenovirus serotypes 3 and 5 were transfected into primary Rhesus monkey kidney cells. The presence of viral DNA sequences was detected in transformed cell lines. All these cell lines expressed the E1A proteins. In addition, Ad5 transformed cells, have the E1B 21 kDa protein located in the nuclear membrane. These cell lines were permissive to the enteric adenovirus serotype 40 but not to serotype 41.