RESUMEN
There is a strong drive worldwide to discover and exploit the therapeutic potential of a large variety of plants. In this work, an alcoholic extract of Helleborus purpurascens (family Ranunculaceae) was investigated for the identification of amino acids and peptides with putative antiproliferative effects. In our work, a separation strategy was developed using solvents of different polarity in order to obtain active compounds. Biochemical components were characterized through spectroscopic (mass spectroscopy) and chromatographic techniques (RP-HPLC and GC-MS). The biological activity of the obtained fractions was investigated in terms of their antiproliferative effects on HeLa cells. Through this study, we report an efficient separation of bioactive compounds (amino acids and peptides) from a plant extract dependent on solvent polarity, affording fractions with unaffected antiproliferative activities. Moreover, the two biologically tested fractions exerted a major antiproliferative effect, thereby suggesting potential anticancer therapeutic activity.
Asunto(s)
Aminoácidos/química , Antineoplásicos/química , Helleborus/química , Proteínas de Plantas/química , Tioninas/química , Aminoácidos/aislamiento & purificación , Aminoácidos/farmacología , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Butanoles , Supervivencia Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Etanol , Dicloruros de Etileno , Células HeLa , Humanos , Extractos Vegetales/química , Proteínas de Plantas/aislamiento & purificación , Proteínas de Plantas/farmacología , Solventes , Tioninas/aislamiento & purificación , Tioninas/farmacologíaRESUMEN
The title compound, C18H24N2Se3, consists of discrete molecules; owing to the presence of strong intramolecular N...Se interactions [N...Se = 2.671 (4) and 2.873 (4) A], the chalcogen Se atoms of the angular Se3 chain exhibit different coordination geometries, i.e. the terminal Se atoms are tricoordinated and exhibit a T-shaped environment of the CNSe2 core [N...Se-Se = 173.73 (9) and 172.29 (9) degrees], while the central Se atom is dicoordinated to the other two Se atoms, with an Se-Se-Se angle of 108.32 (2) degrees.