RESUMEN

Determining structures of large, complex proteins remains challenging, especially for transmembrane proteins, as the protein size increases. Arabidopsis thaliana cellulose synthesis complex is a large, multimeric complex located in the plant cell membrane that synthesizes cellulose microfibrils in the plant cell wall. Despite the biological and economic importance of cellulose and therefore cellulose synthesis, many aspects of the cellulase synthase complex (CSC) structure and function are still unknown. Here, yeast surface display (YSD) is used to determine the full-length expression of A. thaliana cellulose synthase 3 (AtCesA3) fragments. The level of stably-folded AtCesA3 fragments displayed on the yeast surface are determined using flow cytometric analysis of differential surface expression of epitopes flanking the AtCesA3 fragment. This technique provides a fast and simple method for examining folding and expression of protein domains and fragments of complex proteins.

Asunto(s)

Arabidopsis/genética , Pared Celular/genética , Glucosiltransferasas/genética , Complejos Multiproteicos/genética , Arabidopsis/enzimología , Membrana Celular/química , Membrana Celular/genética , Pared Celular/enzimología , Celulosa/química , Regulación Enzimológica de la Expresión Génica , Glucosiltransferasas/química , Complejos Multiproteicos/química , Saccharomyces cerevisiae/genética

RESUMEN

New developments in PET/CT technology have enabled the commercial availability of continuous bed motion (CBM) acquisition methods. This technology has some potential performance benefits compared to standard step and shoot (SS) imaging, however, this technology has not been assessed with regard to quantitative and image quality parameters compared to traditional SS techniques. This study seeks to compare clinically relevant quantitative and image quality parameters using CBM and SS data collection methods with the intent of providing assistance in making educated decisions regarding imaging protocol development when using CBM technology versus SS imaging.