RESUMEN
Bovine respiratory disease causes significant economic losses in both beef and dairy calf industries. Although multi-factorial in nature, the disease is characterized by an acute fibrinous lobar pneumonia typically associated with the isolation of Mannheimia haemolytica. M. haemolytica A1 and A6 are the two most commonly isolated serotypes from cattle, however, the majority of vaccines have not demonstrated cross-serotype protection. In the current study, the efficacy of a novel, attenuated live vaccine, containing both M. haemolytica serotype A1 and Pasteurella multocida, was evaluated in calves challenged with M. haemolytica serotype A6. Although the challenge was more severe than expected, vaccinated calves had reduced clinical scores, lower mortality, and significantly lower lung lesion scores compared to the placebo-vaccinated control group. The results demonstrate that vaccination with an attenuated live vaccine containing M. haemolytica serotype A1 can protect calves against clinical disease following challenge with M. haemolytica serotype A6.
Asunto(s)
Vacunas Bacterianas/inmunología , Enfermedades de los Bovinos/prevención & control , Protección Cruzada/inmunología , Mannheimia haemolytica/inmunología , Pasteurella multocida/inmunología , Pasteurelosis Neumónica/prevención & control , Animales , Proteínas Bacterianas/genética , Proteínas Bacterianas/inmunología , Proteínas Bacterianas/metabolismo , Vacunas Bacterianas/administración & dosificación , Secuencia de Bases , Bovinos , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/microbiología , Exotoxinas/genética , Exotoxinas/inmunología , Exotoxinas/metabolismo , Mannheimia haemolytica/clasificación , Mannheimia haemolytica/genética , Mannheimia haemolytica/patogenicidad , Datos de Secuencia Molecular , Pasteurella multocida/genética , Pasteurelosis Neumónica/inmunología , Pasteurelosis Neumónica/microbiología , Pasteurelosis Neumónica/mortalidad , Serotipificación , Resultado del Tratamiento , Vacunación/veterinaria , Vacunas Atenuadas/administración & dosificación , Vacunas Atenuadas/inmunologíaRESUMEN
Necrotic enteritis is a potentially fatal multifactorial disease of chickens, which under commercial conditions is often associated with increased levels of mortality and reduced bird performance. The safety and efficacy of a Clostridium perfringens type A alpha-toxoid (Netvax™) formulated as an oil emulsion was investigated, following maternal immunization of broiler breeder hens, housed under commercial conditions, by the intramuscular route. A total of 11,234 hens were vaccinated across two integrated poultry sites. The vaccine was safe with no systemic reactions or adverse effects on bird performance detected. Vaccination resulted in a significant increase in anti-alpha toxin antibody in the hen that was maintained throughout the study, and subsequently transferred to their progeny throughout the laying period via egg yolk. Chicks hatched from eggs produced from vaccinated hens were shown to have reduced mortality specifically related to progeny flocks where gross gut lesions associated with necrotic enteritis were observed in control chicks. Further, whilst C. perfringens was isolated from control chicks with necrotic enteritis lesions, no such isolations were made at these time points from chicks from vaccinated hens. These results indicate that, under commercial conditions, maternal vaccination with Netvax™ can help to control losses related to necrotic enteritis.
Asunto(s)
Toxinas Bacterianas/efectos adversos , Vacunas Bacterianas/efectos adversos , Proteínas de Unión al Calcio/efectos adversos , Infecciones por Clostridium/veterinaria , Enteritis/veterinaria , Enfermedades de las Aves de Corral/prevención & control , Toxoides/efectos adversos , Fosfolipasas de Tipo C/efectos adversos , Animales , Anticuerpos Antibacterianos/inmunología , Toxinas Bacterianas/administración & dosificación , Vacunas Bacterianas/administración & dosificación , Proteínas de Unión al Calcio/administración & dosificación , Pollos , Infecciones por Clostridium/inmunología , Infecciones por Clostridium/prevención & control , Clostridium perfringens/fisiología , Enteritis/prevención & control , Inyecciones Intramusculares/veterinaria , Intestinos/microbiología , Intestinos/patología , Necrosis/prevención & control , Necrosis/veterinaria , Enfermedades de las Aves de Corral/inmunología , Toxoides/administración & dosificación , Resultado del Tratamiento , Fosfolipasas de Tipo C/administración & dosificación , Vacunación/métodos , Vacunación/veterinariaRESUMEN
In horses, natural infection confers long lasting protective immunity characterised by mucosal IgA and humoral IgGa and IgGb responses. In order to investigate the potential of locally administered vaccine to induce a protective IgA response, responses generated by vaccination with an immunostimulating complex (ISCOM)-based vaccine for equine influenza (EQUIP F) containing A/eq/Newmarket/77 (H7N7), A/eq/Borlänge/91 (H3N8) and A/eq/Kentucky/98 (H3N8) using a systemic prime/mucosal boost strategy were studied. Seven ponies in the vaccine group received EQUIP F vaccine intranasally 6 weeks after an initial intramuscular immunisation. Following intranasal boosting a transient increase in virus-specific IgA was detected in nasal wash secretions. Aerosol challenge with the A/eq/Newmarket/1/93 reference strain 4 weeks after the intranasal booster resulted in clinical signs of infection and viral shedding in seven of seven influenza-naive control animals whereas the seven vaccinated ponies had statistically significantly reduced clinical signs and duration of virus excretion. Furthermore, following this challenge, significantly enhanced levels of virus-specific IgA were detected in the nasal washes from vaccinated ponies compared with the unvaccinated control animals. These data indicate that the intranasal administration of EQUIP F vaccine primes the mucosal system for an enhanced IgA response following exposure to live influenza virus.
Asunto(s)
Enfermedades de los Caballos/prevención & control , ISCOMs/inmunología , Subtipo H3N8 del Virus de la Influenza A/inmunología , Vacunas contra la Influenza/administración & dosificación , Vacunas contra la Influenza/inmunología , Infecciones por Orthomyxoviridae/prevención & control , Infecciones por Orthomyxoviridae/veterinaria , Animales , Anticuerpos Antivirales/sangre , Femenino , Enfermedades de los Caballos/inmunología , Caballos/inmunología , ISCOMs/administración & dosificación , Inmunoglobulina A/sangre , Inmunoglobulina G/sangre , Masculino , Infecciones por Orthomyxoviridae/inmunología , Esparcimiento de VirusRESUMEN
Protective responses generated by vaccination with an immuno-stimulating complex (ISCOM)-based vaccine for equine influenza (EQUIP F), containing a new 'American lineage' H3N8 virus, were studied. Seven ponies in the vaccine group received two intramuscular injections of EQUIP F given 6 weeks apart. Aerosol challenge with an A/eq/Newmarket/1/93 reference strain 4 weeks after booster vaccination resulted in clinical signs of infection and viral shedding in 7 influenza-naive control animals whereas the vaccinated ponies were significantly protected from both clinical signs and virus excretion. Influenza virus-specific IgG responses in serum following immunisation with the ISCOM vaccine were predominantly of the IgGa and IgGb sub-isotypes, a pattern similar to that generated by equine influenza virus infection. However, in contrast to the response following infection, virus-specific antibody responses in nasal washes following immunisation were characterised by the presence of IgG but not IgA.These results demonstrated that an ISCOM-based vaccine containing A/eq/Kentucky/98 provides strong protective immunity against challenge with an 'American lineage' H3N8 reference virus.
Asunto(s)
Enfermedades de los Caballos/inmunología , Enfermedades de los Caballos/virología , ISCOMs/inmunología , Subtipo H3N8 del Virus de la Influenza A , Virus de la Influenza A/inmunología , Vacunas contra la Influenza/inmunología , Infecciones por Orthomyxoviridae/veterinaria , Animales , Anticuerpos Antivirales/biosíntesis , Femenino , Hemólisis , Enfermedades de los Caballos/prevención & control , Caballos , Inmunización Secundaria/veterinaria , Inmunoglobulina A/metabolismo , Inmunoglobulina G/sangre , Masculino , Mucosa Nasal/inmunología , Mucosa Nasal/virología , Infecciones por Orthomyxoviridae/inmunología , Infecciones por Orthomyxoviridae/prevención & control , Infecciones por Orthomyxoviridae/virología , Factores de Tiempo , Vacunación/veterinariaRESUMEN
The efficacy of a live attenuated anti-coccidial vaccine, Paracox-5, administered to 1-day-old chicks was investigated by assessing protection against changes in weight gain following virulent challenge. Vaccinated birds were challenged independently 28 days later with each of the component species (Eimeria acervulina, Eimeria maxima, Eimeria mitis or Eimeria tenella), and protection was demonstrated against associated reduction in weight gain and lesion formation. In addition, an improvement in bird performance, in terms of feed conversion ratio, was also observed following vaccination. Furthermore, under conditions designed to more closely mimic those in the field and using hatchery spray administration, protection against a mixed virulent challenge introduced by 'seeder birds' was demonstrated evenly across a flock of broiler birds within 21 days after vaccination. These data demonstrate that Paracox-5 vaccine will protect broiler chickens against the adverse effects on performance induced by Eimeria spp.
Asunto(s)
Coccidiosis/inmunología , Coccidiosis/prevención & control , Eimeria/inmunología , Enfermedades de las Aves de Corral/inmunología , Enfermedades de las Aves de Corral/prevención & control , Vacunas Antiprotozoos/inmunología , Vacunas Atenuadas/inmunología , Administración por Inhalación , Animales , Pollos/inmunología , Pollos/parasitología , Coccidiosis/veterinaria , Enfermedades de las Aves de Corral/parasitología , Vacunas Antiprotozoos/administración & dosificación , Vacunación/veterinaria , Vacunas Atenuadas/administración & dosificación , Aumento de PesoRESUMEN
Twenty-five Ayrshire/Friesian cows were vaccinated once with a new combined vaccine against rotavirus, coronavirus and Escherichia coli F5 (K99) or given a saline placebo 31 days before the first expected calving date. Blood samples were taken from the cows at intervals from vaccination until seven days after calving and from their calves up to 28 days after birth, and colostrum and milk samples were collected from the cows at intervals for 28 days after calving. There was a significant increase in the mean specific antibody titre against all three antigens in the serum of the vaccinated animals (even in the presence of pre-existing antibody) which was accompanied by increased levels of protective antibodies to rotavirus, coronavirus and E coli F5 (K99) in their colostrum and milk for at least 28 days.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Anticuerpos Antivirales/sangre , Enfermedades de los Bovinos/prevención & control , Calostro/inmunología , Leche/inmunología , Vacunación/veterinaria , Animales , Animales Recién Nacidos/inmunología , Anticuerpos Antibacterianos/análisis , Anticuerpos Antivirales/análisis , Bovinos , Coronavirus/inmunología , Escherichia coli/inmunología , Femenino , Embarazo , Rotavirus/inmunologíaRESUMEN
In order to investigate the ability of an oil adjuvanted vaccine containing bovine coronavirus antigen to enhance lactogenic immunity in the calf, pregnant cows and heifers were vaccinated and specific virus neutralising antibody levels determined in serum, colostrum and milk. Pre-existing antibody titres (as a result of natural infection) in the serum of these animals were found to be significantly increased as a result of a single shot vaccination carried out between 2 and 12 weeks before calving. This was reflected in a similar increase in the titre and duration of specific antibody in milk and colostrum that was passed on to the calves. The overall response observed was highly dependent on an adequate antigen payload being incorporated within the single dose vaccine. No abnormal local or systemic reactions were observed as a result of vaccination. It is hoped that this approach will lead to the production of a superior commercial vaccine for the protection of neonatal calves against enteric coronavirus infection.
Asunto(s)
Anticuerpos Antivirales/análisis , Calostro/inmunología , Coronavirus/inmunología , Leche/inmunología , Vacunas Virales/inmunología , Animales , Antígenos Virales/inmunología , Bovinos , Relación Dosis-Respuesta Inmunológica , Femenino , Embarazo , VacunaciónRESUMEN
AIMS: To evaluate the clinical performance of enzyme immunoassays for IgG and IgM antibodies to Toxoplasma gondii based on enhanced chemiluminescence. METHODS: Classification of routine clinical samples from the originating laboratories was compared with that obtained using the chemiluminescence based assays. Resolution of discordant results was achieved by testing in alternative enzyme immunoassays (IgM) or by an independent laboratory using the dye test (IgG). RESULTS: Compared with resolved data, the IgM assay was found to be highly specific (100%) with a cut off selected to give optimal performance with respect to both the early detection of specific IgM and the detection of persistent levels of specific IgM (sensitivity 98%). Compared with resolved data, the IgG assay was shown to have a sensitivity and a specificity of 99.4%. CONCLUSIONS: The Amerlite Toxo IgM assay possesses high levels of sensitivity and specificity. Assay interference due to rheumatoid factor like substances is not a problem. The Amerlite Toxo IgG assay possesses good sensitivity and specificity, but is less sensitive for the detection of seroconversion than methods detecting both IgG and IgM.
Asunto(s)
Anticuerpos Antiprotozoarios/sangre , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Toxoplasma/inmunología , Toxoplasmosis/diagnóstico , Animales , Femenino , Humanos , Técnicas para Inmunoenzimas , Mediciones Luminiscentes , Factor Reumatoide/inmunología , Sensibilidad y EspecificidadRESUMEN
Production of diarrhea in neonatal calves by enterotoxigenic Escherichia coli depends on its ability to attach to the epithelial cells of the intestine via surface adhesins called pili or fimbriae and to secrete enterotoxins. The most important of these fimbriae are designated K99 and F41. We produced and characterized a murine monoclonal antibody specific to F41. This monoclonal antibody and a K99-specific monoclonal antibody were used to develop sensitive and specific passive hemagglutination and capture enzyme-linked immunosorbent assays (ELISAs) for detection and quantitation of F41 and K99 antigens in E. coli cultures and culture supernatants. The capture ELISA systems exhibited excellent sensitivity and specificity, whereas the passive hemagglutination systems appeared to be oversensitive. The ability of the capture ELISAs to detect K99 and F41 fimbrial antigens in fecal specimens from calves was evaluated. Fimbrial antigens were detected in six of six specimens from scouring calves but not in four of four specimens from nonscouring calves.
Asunto(s)
Antígenos Bacterianos/análisis , Antígenos de Superficie/análisis , Proteínas de la Membrana Bacteriana Externa/análisis , Toxinas Bacterianas , Proteínas de Escherichia coli , Escherichia coli/inmunología , Fimbrias Bacterianas/inmunología , Animales , Anticuerpos Antibacterianos , Anticuerpos Monoclonales , Bovinos , Enfermedades de los Bovinos/diagnóstico , Diarrea/diagnóstico , Diarrea/veterinaria , Ensayo de Inmunoadsorción Enzimática , Infecciones por Escherichia coli/diagnóstico , Infecciones por Escherichia coli/veterinaria , Heces/microbiología , Pruebas de HemaglutinaciónRESUMEN
Passive immunity against enteric viral infections is dependent upon the continual presence in the gut lumen of a protective level of specific antibodies. This article examines methods currently used to enhance the titre and duration of specific antibody in the mammary secretions of cows and pigs, with particular reference to rotavirus and coronavirus infections. In addition, some of the potential problems to be found in attempting to produce vaccines against these viral infections are outlined.
Asunto(s)
Infecciones por Coronaviridae/veterinaria , Inmunidad Materno-Adquirida , Leche/inmunología , Infecciones por Rotavirus/veterinaria , Animales , Animales Recién Nacidos , Bovinos , Enfermedades de los Bovinos/inmunología , Enfermedades de los Bovinos/prevención & control , Infecciones por Coronaviridae/inmunología , Infecciones por Coronaviridae/prevención & control , Diarrea/inmunología , Diarrea/prevención & control , Diarrea/veterinaria , Femenino , Inmunoglobulina A Secretora/inmunología , Inmunoglobulina G/inmunología , Embarazo , Infecciones por Rotavirus/inmunología , Infecciones por Rotavirus/prevención & control , Porcinos , Enfermedades de los Porcinos/inmunología , Enfermedades de los Porcinos/prevención & control , VacunaciónRESUMEN
Using an ELISA for the detection of virus-specific immune complexes, ten cows were found to be shedding bovine enteric coronavirus. The shedding patterns from five of these animals were followed for a period of 12 weeks, and all were found to be chronically shedding virus. Despite the presence of both faecal and serum antibody the infection was not cleared; therefore, the role of cell-mediated immunity (CMI) was investigated by immunosuppressing the chronically shedding cows with dexamethasone. No major role for CMI in maintaining the chronic infection could be determined, although immunosuppression did result in a temporary reduction in the shedding of virus-specific immune complexes.
Asunto(s)
Complejo Antígeno-Anticuerpo , Enfermedades de los Bovinos/inmunología , Infecciones por Coronaviridae/veterinaria , Coronaviridae/inmunología , Heces/inmunología , Animales , Anticuerpos Antivirales/análisis , Antígenos Virales , Bovinos , Enfermedades de los Bovinos/microbiología , Línea Celular , Coronaviridae/fisiología , Infecciones por Coronaviridae/inmunología , Infecciones por Coronaviridae/microbiología , Dexametasona/farmacología , Ensayo de Inmunoadsorción Enzimática , Heces/microbiología , Riñón , Recuento de Leucocitos , Activación de Linfocitos/efectos de los fármacos , Neutrófilos/fisiología , Factores de Tiempo , Replicación Viral/efectos de los fármacosRESUMEN
Spleen cells from a calf hyperimmunized with bovine enteric coronavirus were fused with nonproducer mouse plasmacytoma cells. Stable hybridoma lines secreting bovine immunoglobulins were obtained. One line secreted monoclonal bovine immunoglobulin G2, which reacted specifically with bovine enteric coronavirus in an enzyme-linked immunosorbent assay, inhibited virus hemagglutination, and precipitated a structural polypeptide with a molecular weight of 26,000 daltons.
Asunto(s)
Anticuerpos Monoclonales/biosíntesis , Anticuerpos Antivirales/biosíntesis , Coronaviridae/inmunología , Hibridomas/inmunología , Inmunoglobulina G/biosíntesis , Animales , Bovinos , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Pruebas de Hemaglutinación , Inmunización/veterinaria , Masculino , Ratones , Peso Molecular , Bazo/inmunologíaRESUMEN
A field trial was designed to determine the efficacy of a combination rotavirus-coronavirus/Escherichia coli vaccine on dairy farms in southwestern Ontario. In Part A of the trial, 321 cows on 15 farms were randomly assigned to either vaccination or placebo groups. On eight farms, 50% of the dams were vaccinated, while on the other seven farms, 80% of the dams were vaccinated. In Part B of the trial, 26 farms were randomly assigned to either a total vaccination program or to no vaccination program. Mortality, disease occurrence and weight gains were recorded on all calves for the first two weeks of life. In Part A, 23.5% of all calves were treated in the first two weeks of life, 20.9% were treated specifically for scours and 3.6% of live-born calves died. Enteropathogenic E. coli was identified on 13 of the 15 farms, rotavirus on 11 and coronavirus on ten. At least one of the three potential pathogens was found on every farm. There were no significant differences between calves from placebo-treated and vaccine-treated dams with regard to the proportion treated for all diseases, or for scours, or the proportion which died. Neither were there differences in days to first treatment for all diseases (seven days on average), days to first scour (6.7 days), duration of treatments (3.9 days for all diseases, 3.7 days for scours), or estimated weight gains (0.5 kg/day to 14 days). These results were not altered when the presence or absence of enteropathogenic E. coli, rotavirus or coronavirus on the premises was accounted for.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Vacunas Bacterianas , Enfermedades de los Bovinos/prevención & control , Infecciones por Coronaviridae/veterinaria , Infecciones por Escherichia coli/veterinaria , Infecciones por Rotavirus/veterinaria , Vacunas Virales , Animales , Bovinos , Ensayos Clínicos como Asunto , Coronaviridae/inmunología , Infecciones por Coronaviridae/prevención & control , Diarrea/prevención & control , Diarrea/veterinaria , Método Doble Ciego , Escherichia coli/inmunología , Infecciones por Escherichia coli/prevención & control , Femenino , Rotavirus/inmunología , Infecciones por Rotavirus/prevención & control , Vacunas Virales/inmunologíaRESUMEN
This study demonstrates that populations of defective interfering Semliki Forest virus (DI SEV) are heterogeneous particularly in respect of their interference properties. Interference was quantified by two assays, one measuring inhibition of the yield of infectious progeny virus, and the other measuring reduction in virus-directed RNA synthesis; for 11 different DI SFV preparations a ratio of the two interference titres was calculated. These ratios varied up to 46-fold indicating that each DI virus preparation contained an interference activity that varied independently of the other. However, sister stocks made from the same parental inoculum had similar properties. The effects of different DI virus preparations on other parameters (virus polypeptide synthesis, yield of DI virus and yield of infectious virus) were investigated using inocula with interference titres standardized by either assay. Co-inoculation of L929 cells with 50 p.f.u. SFV showed that these parameters varied independently of each other and of the DI virus inoculum. There was no correlation with the number of undiluted passages each DI stock had received. Direct evidence of physical heterogeneity was demonstrated by metrizamide density gradient centrifugation. Although infecting virus sedimented as a narrow band, DI SFV was distributed over a broad region of the gradient. Its position on the gradient indicated that DI SFV has a higher nucleic acid: protein ratio than standard virus. DI virus progeny obtained by using fractions of the gradient as inoculum were as heterogeneous as the unfractionated parent, confirming that DI viruses retain heterogeneity on passage.
Asunto(s)
Virus Defectuosos/fisiología , Virus de los Bosques Semliki/fisiología , Interferencia Viral , Animales , Células Cultivadas , Embrión de Pollo , Cricetinae , Temperatura , Proteínas Virales/biosíntesis , Replicación ViralRESUMEN
The prevalence of rotavirus and coronavirus shedding by adult cows was investigated using capture enzyme-linked immunosorbent assays. Fecal samples from 121 cows in a single herd were tested for the presence of rotavirus and coronavirus, either free or complexed with immunoglobulin. Free rotavirus was not detected in any samples while rotavirus-immunoglobulin complexes were detected in 53 of 121 (44%) samples tested. In contrast, free coronavirus was detected in six (5%) samples and coronavirus-immunoglobulin complexes were detected in 85 (70%) of the samples tested. Thus it appears that subclinical infection of cows by either of these viruses is common, possibly providing a source for infection of the neonate. These assays may therefore provide important information regarding the epidemiology of enteric virus infections and suggest means of improving management to prevent epidemics of neonatal diarrhea.
Asunto(s)
Antígenos Virales/análisis , Bovinos/inmunología , Coronaviridae/inmunología , Heces/microbiología , Rotavirus/inmunología , Animales , Ensayo de Inmunoadsorción Enzimática/veterinaria , FemeninoRESUMEN
Monoclonal antibodies reactive with three different viral polypeptides were evaluated singly and in combination as the capture antibody(s) in an enzyme-linked immunosorbent assay system for the detection of bovine enteric coronavirus. Similar levels of sensitivity were found for all combinations tested. A sensitive, highly specific, and reproducible assay for the detection of bovine enteric coronavirus was developed, using a mixture of two of these monoclonal antibodies reactive with antigenic components either external or internal to the virion. These monoclonal antibodies were bound indirectly to 96-well plates via rabbit anti-mouse immunoglobulin. After sample application and incubation, virus was detected by using rabbit anti-coronavirus peroxidase conjugate followed by enzyme substrate and chromagen. Fecal samples from a single herd of cows were screened for the presence of coronavirus by this assay. Five percent of clinically normal cows were found to be shedding coronavirus.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Enfermedades de los Bovinos/microbiología , Infecciones por Coronaviridae/veterinaria , Coronaviridae/aislamiento & purificación , Ensayo de Inmunoadsorción Enzimática , Técnicas para Inmunoenzimas , Animales , Bovinos , Coronaviridae/inmunología , Infecciones por Coronaviridae/microbiología , Diarrea/microbiología , Diarrea/veterinaria , Heces/microbiología , ConejosRESUMEN
Purified coronavirus, detergent extracts of purified coronavirus, and virus-infected Madin-Darby bovine kidney cells were evaluated as antigen substrates in enzyme-linked immunosorbent assay (ELISA) and passive hemagglutination systems. Only detergent-extracted and -unextracted, purified viruses were reactive as antigen substrates in ELISA, whereas all three antigen preparations could be used for sensitization of erythrocytes in the passive hemagglutination assay. The passive hemagglutination system with infected cell extracts exhibited a similar level of sensitivity and specificity to the ELISA system employing purified coronavirus but enabled 300 times more tests to be performed per volume of virus-infected cell culture.
Asunto(s)
Anticuerpos Antivirales/análisis , Antígenos Virales/inmunología , Coronaviridae/inmunología , Animales , Bovinos , Infecciones por Coronaviridae/inmunología , Parto Obstétrico , Ensayo de Inmunoadsorción Enzimática , Femenino , Pruebas de Hemaglutinación , Periodo Posparto , EmbarazoRESUMEN
The majority of mice inoculated with a mixture of a lethal dose of virulent Semliki Forest virus (SFV) strain ts+ and defective-interfering (DI) SFV remained completely health, with virus infectivity levels in brain tissue reduced by 99.9%. The results of previous studies had suggested that these effects were primarily the result of the intrinsic interfering capacity of DI virus rather than of host defense responses. Because SFV strain ts+ and an avirulent strain of SFV have clearly distinguishable histopathologic effects in brain tissue, the capability of DI virus to change the virulent into the avirulent form of the disease was examined. Modulation of strain ts+ virus infection by DI virus was accompanied by a complete absence of histopathologic changes despite significant levels of infectious virus and thus differed qualitatively from infection with avirulent SFV. These results provide further evidence that the interference is not mediated through stimulation of an immune cell infiltration.
Asunto(s)
Virus Defectuosos/fisiología , Virus de los Bosques Semliki/fisiología , Infecciones por Togaviridae/microbiología , Interferencia Viral , Animales , Encéfalo/patología , Masculino , Ratones , Necrosis , Neuroglía/patología , Médula Espinal/patología , Infecciones por Togaviridae/patologíaRESUMEN
We describe a simple, rapid and reproducible assay for defective-interfering Semliki Forest virus (DI SFV) which is based on the inhibition of synthesis of virus-specified RNAs in SFV-infected cells. Using the assay, we have been able to show that DI virus is generated by a single passage in baby hamster kidney (BHK) cells in an inoculum which contained no detectable DI virus and we have calculated the u.v. target size of the interfering activity.