RESUMEN
Serum amyloid A (SAA) and Haptoglobin (Hp) are acute phase proteins, produced during inflammation, such as placentitis. In horses, SAA and SAA1 are protein coding genes. Objectives were to analyze SAA and Hp concentrations and relative abundance of SAA, SAA1 and Hp mRNA transcript in maternal and fetal tissues after experimental induction of placentitis or mares of a control group. Serum Amyloid A family proteins were in marked abundance in the stroma of the endometrium and chorioallantois associated with inflammatory cells. Maternal plasma SAA concentrations were greater (P = 0.01) in mares with experimentally induced placentitis compared to those of the control group. Maternal Hp from the groups were not different, but fetal Hp concentrations of mares with experimentally induced placentitis were greater (P = 0.02). Maternal plasma SAA and Hp concentrations were greater than fetal plasma concentrations in mares with experimentally induced placentitis (P < 0.05). Relative abundance of SAA mRNA transcript was greater in the maternal, fetal liver and chorioallantois of mares with experimentally induced placentitis (P < 0.05) compared to those in the control group. Interestingly, relative abundance of SAA1 mRNA transcript was greater in the chorioallantois of mares with experimentally induced placentitis (P < 0.05). The SAA and Hp concentrations, therefore, were greater in mares with induced placentitis. Furthermore, relative abundance of SAA1 mRNA transcript is specifically greater in the chorioallantois of mares with placentitis, which warrants further studies to elucidate the immunological response of SAA1 in the chorioallantois of mares with placentitis.
Asunto(s)
Haptoglobinas/metabolismo , Enfermedades de los Caballos/sangre , Enfermedades Placentarias/veterinaria , Proteína Amiloide A Sérica/metabolismo , Infecciones Estreptocócicas/veterinaria , Animales , Femenino , Feto , Enfermedades de los Caballos/inducido químicamente , Enfermedades de los Caballos/microbiología , Caballos , Enfermedades Placentarias/sangre , Enfermedades Placentarias/microbiología , Embarazo , Infecciones Estreptocócicas/sangre , Infecciones Estreptocócicas/metabolismo , Streptococcus equiRESUMEN
Encephalitozoon cuniculi is an obligate intracellular microsporidian parasite that causes clinical and subclinical infection in many species. It is also considered a potential zoonotic disease. In the present study, a serological survey was conducted using samples from pet cats using an ELISA with a confirmed positive control sample from a cat with E. cuniculi related uveitis. Thirty-four of 127 serum samples or 26.8% were found to demonstrate reactivity with titers ranging from 1:32 to 1:1024. There were no significant differences by age or sex (p=0.99 and p=0.32, respectively). This is the first description of the use of an enzyme linked immunosorbent assay for the detection of E. cuniculi antibodies in cats in the United States.
RESUMEN
Antemortem diagnosis of avian aspergillosis is very challenging. Diagnostic assays using blood samples would aid in an early and more definitive diagnosis. In the current study, detection of anti-Aspergillus antibodies, Aspergillus antigen, and Aspergillus toxin (fumigaclavine A), protein electrophoresis and measurement of acute-phase protein concentrations were performed on serum of 18 adult and plasma of 21 juvenile gyr-saker hybrid falcons (Falco rusticolus x Falco cherrug). Adult (n = 15) and juvenile (n = 18) falcons were experimentally inoculated with different dosages of the same strain of Aspergillus fumigatus and an additional three falcons from each age group were used as uninfected control animals. Blood samples were collected prior to inoculation and at 28 days postinoculation. Of the 33 inoculated falcons, 16 demonstrated clinical signs (vomiting, greenish urates, dyspnea, ruffled feathers) commonly associated with aspergillosis and in 14 falcons necropsy revealed aspergillosis granulomas confirmed by mycology and histopathology. Positive galactomannan results were rare, with only 3/15 positive samples from adult falcons and none in the juvenile birds. Most of the inoculated falcons showed an increase of serum amyloid A (66.7%) and haptoglobin (70.4%), but fumigaclavine A was not detected in the blood from any of the experimental animals. Elevated antibody indices were detected in 96.7% of the inoculated birds, but also in 66.7% of the controls. Significant decreases in albumin:globulin ratio were obvious in 81.5% of the inoculated birds, including 100% of the birds with granulomas. Blood from falcons with granulomas demonstrated significantly increased concentration values of alpha 2 and ß globulins, decreased percentages of prealbumin and albumin, and increased percentages of alpha 2 and ß globulins compared to inoculated falcons without granulomas. In conclusion, acute-phase proteins and the electrophoretic profile of birds challenged with A. fulmigatus show significant alterations, which in combination with other diagnostic procedures, assist in the early diagnosis of avian aspergillosis.
Asunto(s)
Anticuerpos Antifúngicos/sangre , Aspergillus fumigatus , Enfermedades de las Aves/sangre , Falconiformes , Técnicas para Inmunoenzimas/veterinaria , Aspergilosis Pulmonar/veterinaria , Pruebas Serológicas/veterinaria , Envejecimiento , Animales , Enfermedades de las Aves/microbiología , Aspergilosis Pulmonar/diagnóstico , Aspergilosis Pulmonar/microbiologíaRESUMEN
Spontaneous atherosclerosis is common in psittaciformes, and clinical signs associated with flow-limiting stenosis are encountered in pet birds. Nevertheless, a psittacine model of atherosclerosis has not been developed for research investigations. Sixteen captive-bred Quaker parrots (Myiopsitta monachus) were used in this study. While 4 control birds were fed a maintenance diet, 12 other birds were fed an atherogenic diet composed of 1% cholesterol controlling for a calorie-to-protein ratio for periods ranging from 2 to 8 months. The birds were euthanized at the end of their respective food trial period. Histopathology, transmission electron microscopy, and cholesterol measurement were performed on the ascending aorta and brachiocephalic and pulmonary arteries. Plasma lipoproteins, cholesterol, and triglycerides were also measured on a monthly basis. Significant atherosclerotic lesions were induced within 2 months and advanced atherosclerotic lesions within 4 to 6 months. The advanced lesions were histologically similar to naturally occurring lesions identified in the same parrot species with a lipid core and a fibrous cap. Ultrastructurally, there were extracellular lipid, foam cell, and endothelial changes. Arterial cholesterol content increased linearly over time. Plasma cholesterol and low-density lipoprotein (LDL) significantly increased over time by an average of 5- and 15-fold, respectively, with a shift from high-density lipoprotein to LDL as the main plasma lipoprotein. Quaker parrots also exhibited high plasma cholesteryl ester transfer protein activity that increased, although not significantly, over time. This experiment demonstrates that in Quaker parrots fed 1% cholesterol, advanced atherosclerosis can be induced relatively quickly, and lesions resemble those found in other avian models and humans.
Asunto(s)
Aterosclerosis/veterinaria , Enfermedades de las Aves/patología , Colesterol/sangre , Dieta Aterogénica/veterinaria , Loros , Animales , Aorta/metabolismo , Aorta/patología , Aterosclerosis/metabolismo , Aterosclerosis/patología , Enfermedades de las Aves/etiología , Enfermedades de las Aves/metabolismo , Venas Braquiocefálicas/metabolismo , Venas Braquiocefálicas/patología , Dieta Aterogénica/efectos adversos , Modelos Animales de Enfermedad , Femenino , Lípidos/sangre , Lipoproteínas LDL/sangre , Masculino , Arteria Pulmonar/metabolismo , Arteria Pulmonar/patología , Triglicéridos/sangreRESUMEN
BACKGROUND: The stoic nature of alpacas and limitations of current diagnostic tests make early recognition of inflammatory diseases in this species challenging. OBJECTIVES: In a model of mild systemic inflammation, this study evaluated the utility of different clinical and clinicopathologic variables as accurate predictors of inflammation in alpacas. ANIMALS: Twelve clinically healthy alpacas were randomly assigned to equal-sized treatment (TG) and control (CG) groups. After collection of initial blood samples (0 hour), lipopolysaccharide (LPS; 20 µg/kg/24 h) or saline was administered by SC osmotic mini-pumps (OMP) for 96 hours. Additional blood samples were collected at 12, 18, 24, 36, 48, 72, 96, 120, 144, and 240 hours and differential leukocyte counts and concentrations of globulin, albumin, iron, haptoglobin, and serum amyloid A were measured. RESULTS: Mild swelling was observed at OMP implantation sites in both groups. Other clinical signs of systemic inflammation were not observed. Total leukocytes, neutrophils, albumin, and globulin concentrations were not significantly different between groups. Compared with CG-alpacas, TG-alpacas had fewer lymphocytes (P = .0322), more band neutrophils (P = .0087), and higher neutrophil/lymphocyte ratios (P = .0295) during the first 96 hours of the study. During LPS administration, serum iron concentrations were significantly decreased in TG-alpacas (P < .0001). Haptoglobin concentrations of TG-animals exceeded those of CG-animals after removal of OMP (P = .0056). Serum amyloid A was not detectable in alpacas in this study. CONCLUSION AND CLINICAL IMPORTANCE: These results indicated that neutrophil/lymphocyte ratios and serum iron concentrations are early indicators of inflammation in alpacas. Additional research is needed to evaluate the acute phase protein responses of alpacas.
Asunto(s)
Camélidos del Nuevo Mundo/sangre , Inflamación/veterinaria , Lipopolisacáridos/toxicidad , Proteínas de Fase Aguda/genética , Proteínas de Fase Aguda/metabolismo , Animales , Recuento de Células Sanguíneas/veterinaria , Análisis Químico de la Sangre/veterinaria , Inflamación/sangre , Inflamación/diagnóstico , Hierro/sangre , MasculinoRESUMEN
Encephalitozoon cuniculi is an obligate intracellular microsporidian parasite that can result in clinical and subclinical infection in many species. It is also considered the cause of a potential zoonotic disease. In the present study, a serological survey was conducted using samples from pet dogs in the state of Florida. Twenty-seven of 125 serum samples, or 21.6%, were found to demonstrate reactivity by ELISA, with titers ranging from 1:32 to 1:512. There were no significant differences by age or sex. This is the first report on the detection of E. cuniculi antibodies in dogs in the United States.
Asunto(s)
Anticuerpos Antifúngicos/sangre , Enfermedades de los Perros/epidemiología , Encephalitozoon cuniculi/inmunología , Encefalitozoonosis/veterinaria , Factores de Edad , Animales , Enfermedades de los Perros/inmunología , Enfermedades de los Perros/parasitología , Perros , Encefalitozoonosis/epidemiología , Encefalitozoonosis/inmunología , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Masculino , Mascotas , Estudios Seroepidemiológicos , Estados Unidos/epidemiologíaRESUMEN
The U.S. Fish and Wildlife Service required a chronic dosing study that assessed the health and reproductive effects of tungsten-iron and tungsten-polymer shot in adult game-farm mallards (Anas platyrhynchos) prior to granting permanent approval of the shot for waterfowl hunting. Herein, we present the effects of tungsten-iron and tungsten-polymer shot on various hematologic parameters and metal residue concentrations in the femur, liver, kidneys, and gonads. Thirty-two-bird groups (sexes equal) of adult mallards were dosed orally with eight #4 steel shot (control), eight #4 tungsten-iron shot, or eight #4 tungsten-polymer shot on days 0, 30, 60, 90, and 120 of a 150 day trial (26 January 1998 to 25 June 1998). An additional 12 mallards (sexes equal) received eight #4 lead shot (positive control) on day 0 of the study. Lead-dosed mallards had significantly decreased hematocrit, hemoglobin concentration, and whole-blood delta aminolevulinic acid dehydratase activity on day 7, as well as significant changes in a number of plasma chemistry parameters compared to ducks in the control, tungsten-iron, or tungsten-polymer groups. Mallards dosed with tungsten-iron or tungsten-polymer shot had occasional significant differences in hematocrit and plasma chemistry values when compared to control mallards over the 150 day period, but these changes were not considered to be indicative of deleterious effects. Low concentrations of tungsten were detected in gonad and kidney samples from males and females and in liver samples from females dosed with tungsten-polymer shot. Tungsten was also detected in femur samples from tungsten-polymer-dosed mallards. Higher concentrations of tungsten were detected in femur, liver, kidney, and gonad samples from tungsten-iron-dosed ducks. Tungsten-iron or tungsten-polymer shot repeatedly administered to adult mallards did not cause adverse hematological effects during the 150 day trial. Concentrations of tungsten in the femur, liver, kidneys, and gonads were generally higher in tungsten-iron-dosed ducks when compared to tungsten-polymer-dosed ducks.
Asunto(s)
Enfermedades de las Aves/sangre , Caprolactama/análogos & derivados , Residuos de Medicamentos/análisis , Patos , Hierro/toxicidad , Tungsteno/toxicidad , Animales , Enfermedades de las Aves/inducido químicamente , Enfermedades de las Aves/patología , Análisis Químico de la Sangre/veterinaria , Caprolactama/toxicidad , Esquema de Medicación , Enzimas/sangre , Enzimas/efectos de los fármacos , Femenino , Fémur/química , Fémur/patología , Gónadas/química , Gónadas/patología , Hematócrito/veterinaria , Pruebas Hematológicas/veterinaria , Hierro/sangre , Riñón/química , Riñón/patología , Plomo/toxicidad , Hígado/química , Hígado/patología , Masculino , Polímeros/toxicidad , Porfobilinógeno Sintasa/sangre , Porfobilinógeno Sintasa/efectos de los fármacos , Acero/toxicidad , Distribución Tisular , Tungsteno/sangreRESUMEN
Cell-mediated and humoral immune status of free-ranging green turtles (Chelonia mydas) in Hawaii (USA) with and without fibropapillornatosis (FP) were assessed. Tumored and non-tumored turtles from Kaneohe Bay (KB) on the island of Oahu and from FP-free areas on the west (Kona/Kohala) coast of the island of Hawaii were sampled from April 1998 through February 1999. Turtles on Oahu were grouped (0-3) for severity of tumors with 0 for absence of tumors, 1 for light, 2 for moderate, and 3 for most severe. Turtles were weighed, straight carapace length measured and the regression slope of weight to straight carapace length compared between groups (KB0, KB1, KB2, KB3, Kona). Blood was assayed for differential white blood cell count, hematocrit, in vitro peripheral blood mononuclear cell (PBMC) proliferation in the presence of concanavalin A (ConA) and phytohaemagglutinin (PHA), and protein electrophoresis. On Oahu, heterophil/lymphocyte ratio increased while eosinophil/monocyte ratio decreased with increasing tumors score. Peripheral blood mononuclear cell proliferation indices for ConA and PHA were significantly lower for turtles with tumor scores 2 and 3. Tumor score 3 turtles (KB3) had significantly lower hematocrit, total protein, alpha 1, alpha 2, and gamma globulins than the other four groups. No significant differences in immune status were seen between non-tumored (or KB1) turtles from Oahu and Hawaii. There was no significant difference between groups in regression slopes of body condition to carapace length. We conclude that turtles with severe FP are imunosuppressed. Furthermore, the lack of significant difference in immune status between non-tumored (and KB1) turtles from Oahu and Kona/Kohala indicates that immunosuppression may not be a prerequisite for development of FP.
Asunto(s)
Papiloma/veterinaria , Tortugas/inmunología , Animales , Formación de Anticuerpos , Concanavalina A/sangre , Hawaii , Hematócrito/veterinaria , Inmunidad Celular , Activación de Linfocitos , Recuento de Linfocitos/veterinaria , Subgrupos Linfocitarios , Papiloma/sangre , Papiloma/inmunología , Fitohemaglutininas/sangre , Índice de Severidad de la Enfermedad , Tortugas/sangreRESUMEN
The immune competence of green sea turtles (Chelonia mydas) with fibropapillomatosis was assessed using in vitro techniques to measure lymphocyte proliferation in response to mitogens. In comparison with captive, healthy green sea turtles, those afflicted with fibropapillomas demonstrated diminished proliferation with Concanavalin A, phytohemagglutinin (T-cell mitogens), and lipopolysaccharide (B-cell mitogen). Also, markedly decreased proliferative responses to the lymphocyte polyclonal stimulator combination of ionomycin and phorbol myristate acetate were observed. Total circulating white blood cell counts were not statistically different between the two groups, although an overall decrease in lymphocyte number was observed in the papilloma group. The albumin/globulin ratio was decreased in the papilloma group because of decreased albumin and increased gamma globulins.
Asunto(s)
Papiloma/veterinaria , Neoplasias Cutáneas/veterinaria , Tortugas/inmunología , Animales , Concanavalina A/farmacología , Inmunidad Celular , Recuento de Leucocitos/veterinaria , Activación de Linfocitos/efectos de los fármacos , Linfocitos/sangre , Linfocitos/inmunología , Mitógenos/farmacología , Papiloma/inmunología , Fitohemaglutininas/farmacología , Neoplasias Cutáneas/sangre , Neoplasias Cutáneas/inmunología , Tortugas/sangreRESUMEN
OBJECTIVE: To establish hematologic and biochemical reference values for the brown pelican (Pelecanus occidentalis). ANIMALS: 31 captive, healthy, but permanently disabled pelicans and 35 wild-caught, healthy pelicans from a rehabilitation facility on the east coast of Florida. PROCEDURES: Samples of venous blood were collected from each pelican, and hematologic, plasma biochemical, and electrophoretic protein analyses were performed. Student t-tests were used to compare blood values between captive versus wild-caught, adult male versus adult female, and adult versus juvenile pelicans. RESULTS: Hematologic and electrophoretic values were similar between male and female, adult and juvenile, and captive and wild-caught pelicans. Significant sex differences existed for plasma calcium and triglyceride concentrations. Plasma concentrations of calcium, cholesterol, and CO2 content differed between captive and wild-caught adults. No significant differences were found between wild-caught adult and juvenile pelicans. CONCLUSIONS AND CLINICAL RELEVANCE: Our plasma biochemical results are similar to those of other brown pelicans and confamilial species. Additional studies on seabirds are encouraged, as age, sex, reproductive status, feeding habits, and captivity are important variables for health assessment in this and other aquatic species.
Asunto(s)
Aves/sangre , Conservación de los Recursos Naturales , Animales , Calcio/sangre , Dióxido de Carbono/sangre , Colesterol/sangre , Electroforesis en Gel de Agar , Recuento de Eritrocitos/veterinaria , Femenino , Florida , Hematócrito/veterinaria , Recuento de Leucocitos/veterinaria , Masculino , Caracteres Sexuales , Triglicéridos/sangreRESUMEN
Plasma proteins of 139 healthy adult birds of prey from 10 species were separated by electrophoresis to characterize and document normal reference ranges and species-specific electrophoretic patternsand to evaluate the value of this technique for health screening, disease diagnosis, and prognostic indication. Species studied included bald eagle (Haliaeetus leucocephalus), red-tailed hawk (Buteo jamaicensis), barn owl (Tyto alba), great horned owl (Bubo virginianus), turkey vulture (Cathartes aura), Harris' hawk (Parabuteo unicinctus), Stellar's sea eagle (Haliaeetus pelagicus), barred owl (Strix varia), screech owl (Otus asio), and black vulture (Coragyps atratus). Several clinical cases show the diagnostic/therapeutic value of protein electrophoresis in raptors. This study establishes species-specific reference ranges for several birds of prey and discusses the benefit of electrophoresis as a diagnostic technique in health screens, as a diagnostic aid in conjunction with other tests, and as a prognostic indicator in clinical evaluation of raptors.
Asunto(s)
Enfermedades de las Aves/diagnóstico , Electroforesis de las Proteínas Sanguíneas/veterinaria , Rapaces , Animales , Electroforesis de las Proteínas Sanguíneas/normas , Osteomielitis/diagnóstico , Osteomielitis/veterinaria , Pronóstico , Valores de Referencia , Especificidad de la EspecieRESUMEN
Sixteen-bird groups (sexes equal) of adult mallards (Anas platyrhynchos) were orally dosed with eight #4 steel short, eight #4 lead shot, eight BB-size tungsten-iron shot, eight BB-size tungsten-polymer shot, or were sham-dosed and maintained for 30 days (16 January 1996 to 15 February 1996). Half of the lead-dosed ducks (five males, three females) died during the study, whereas no ducks died in the other dosage groups. For lead-dosed ducks, hematocrit and hemoglobin concentration were decreased on day 15 of the trial, but not on day 30. Delta aminolevulinic acid dehydratase activity in lead-dosed ducks was lower when compared to steel-dosed ducks only. Plasma activities of selected enzymes were elevated in lead-dosed ducks when compared to enzyme activities of ducks in the other groups. For lead-dosed ducks, relative heart, liver, and kidney weights increased in comparison to relative weights of those organs of ducks in other groups. Histology of tissues indicated that renal nephrosis accompanied by biliary stasis was present in the eight lead-dosed ducks that died. For the eight lead-dosed ducks that survived, six had mild to severe biliary stasis. Mild biliary stasis was noted in five tungsten-iron dosed ducks and three tungsten-polymer dosed ducks. Amounts of lead in the femur, liver, and kidneys were higher in lead-dosed ducks than in ducks of the other four groups. Small amounts of tungsten were detected in the femur and kidneys of two tungsten-polymer dosed ducks. Higher concentrations of tungsten were detected in the femur, liver, and kidneys of all tungsten-iron dosed ducks. The rate of shot erosion was highest (80%) for the tungsten-polymer shot, followed by tungsten-iron (55%), lead (50%), and steel shot (33%). Results indicated that tungsten-iron or tungsten-polymer shot (8 shot/duck) orally administered to mallards did not adversely affect them during a 30-day trial.
Asunto(s)
Enfermedades de las Aves/inducido químicamente , Patos , Intoxicación por Plomo/veterinaria , Plomo/toxicidad , Acero/toxicidad , Tungsteno/toxicidad , Alanina Transaminasa/sangre , Animales , Aspartato Aminotransferasas/sangre , Enfermedades de las Aves/sangre , Enfermedades de las Aves/mortalidad , Peso Corporal/efectos de los fármacos , Creatina Quinasa/sangre , Femenino , Fémur/química , Molleja de las Aves/patología , Hematócrito/veterinaria , Hemoglobinas/análisis , Riñón/química , Riñón/patología , L-Lactato Deshidrogenasa/sangre , Plomo/análisis , Intoxicación por Plomo/sangre , Intoxicación por Plomo/mortalidad , Hígado/química , Hígado/patología , Masculino , Tamaño de los Órganos/efectos de los fármacos , Intoxicación/sangre , Intoxicación/mortalidad , Intoxicación/veterinaria , Porfobilinógeno Sintasa/sangre , Distribución Aleatoria , Acero/análisis , Tungsteno/análisisRESUMEN
Current serologic tests used to detect antibodies to Neospora caninum require species-specific secondary antibodies, limiting the number of species that can be tested. In order to examine a wide variety of animal species that may be infected with N. caninum, a modified direct agglutination test (N-MAT) similar to the Toxoplasma gondii modified direct agglutination test (T-MAT) was developed. This test measures the direct agglutination of parasites by N. caninum-specific antibodies in serum, thus eliminating the need for secondary host-specific anti-isotype sera. The N-MAT was compared to the indirect fluorescent-antibody test (IFAT) and the enzyme-linked immunosorbent assay (ELISA) with a "gold standard" serum panel from species for which secondary antibodies were available (n = 547). All positive samples tested were from animals with histologically confirmed infections. Up to 16 different species were tested. The N-MAT gave a higher sensitivity (100%) and specificity (97%) than the ELISA (74 and 94%, respectively) and had a higher sensitivity but a lower specificity than the IFAT (98 and 99%, respectively). The reduced specificity of the N-MAT was due to false-positive reactions in testing fetal fluids with particulate matter or severely hemolyzed serum. Overall, the N-MAT proved to be highly sensitive and specific for both naturally and experimentally infected animals, highly reproducible between and within readers, easy to use on large sample sizes without requiring special equipment, and useful in testing serum from any species without modification.
Asunto(s)
Pruebas de Aglutinación/métodos , Coccidiosis/veterinaria , Ensayo de Inmunoadsorción Enzimática/métodos , Técnica del Anticuerpo Fluorescente Indirecta , Neospora/inmunología , Neospora/aislamiento & purificación , Aborto Veterinario/diagnóstico , Aborto Veterinario/inmunología , Pruebas de Aglutinación/estadística & datos numéricos , Animales , Anticuerpos Antiprotozoarios/análisis , Bovinos , Enfermedades de los Bovinos/diagnóstico , Enfermedades de los Bovinos/inmunología , Coccidiosis/diagnóstico , Coccidiosis/inmunología , Ensayo de Inmunoadsorción Enzimática/estadística & datos numéricos , Estudios de Evaluación como Asunto , Femenino , Técnica del Anticuerpo Fluorescente Indirecta/estadística & datos numéricos , Valor Predictivo de las Pruebas , Embarazo , Sensibilidad y Especificidad , Especificidad de la EspecieAsunto(s)
Delfines , Leucemia-Linfoma Linfoblástico de Células Precursoras/veterinaria , Neoplasias del Bazo/veterinaria , Animales , Núcleo Celular/patología , Núcleo Celular/ultraestructura , Cromatina/patología , Cromatina/ultraestructura , Femenino , Ganglios Linfáticos/patología , Microscopía Electrónica , Leucemia-Linfoma Linfoblástico de Células Precursoras/patología , Leucemia-Linfoma Linfoblástico de Células Precursoras/ultraestructura , Neoplasias del Bazo/patología , Neoplasias del Bazo/ultraestructuraRESUMEN
Passive-transfer studies were performed to assess the ability of antibody alone to reduce the frequency and/or severity of necrotizing retinitis caused by murine cytomegalovirus (MCMV) in C57BL/6 mice with retrovirus-induced immunodeficiency syndrome (MAIDS). Initial experiments showed a gradual decline in the ability of mice to initiate humoral immunity during the evolution of MAIDS so that neither MCMV-specific IgM nor IgG could be detected during late-stage MAIDS. Passively administered hyperimmune MCMV immunoglobulin, however, could be detected within the serum of mice with MAIDS for at least 9 days after intraperitoneal injection and protected these animals in preliminary experiments from systemic MCMV disease and death when administered 24 h prior to intraperitoneal challenge with a lethal dose of virus. Nonetheless, passive transfer of hyperimmune MCMV serum to mice with MAIDS failed to reduce intraocular MCMV titers, frequency of retinitis, or severity of retinitis when administered 24 h prior to subretinal MCMV inoculation. Whereas whole eyes of MAIDS animals that received normal mouse serum and were injected subretinally with MCMV had an ocular MCMV titer of 4.3 log10 and a frequency of retinitis of 89% (severity score = 55%), whole eyes of antibody-treated mice with MAIDS had an ocular MCMV titer of 4.3 log10 and a frequency of retinitis of 87% (severity score = 57 %). Passive transfer of a neutralizing MCMV-specific monoclonal antibody also failed to reduce the frequency or severity of MCMV retinitis when administered to mice with MAIDS prior to subretinal MCMV inoculation. Our findings suggest that antibody immunotherapy alone will not be effective therapeutically for cytomegalovirus retinitis in patients with AIDS.
Asunto(s)
Anticuerpos Antivirales/administración & dosificación , Retinitis por Citomegalovirus/prevención & control , Infecciones por Herpesviridae/prevención & control , Inmunización Pasiva/métodos , Síndrome de Inmunodeficiencia Adquirida del Murino/complicaciones , Muromegalovirus/inmunología , Animales , Retinitis por Citomegalovirus/patología , Retinitis por Citomegalovirus/virología , Modelos Animales de Enfermedad , Ensayo de Inmunoadsorción Enzimática , Femenino , Infecciones por Herpesviridae/etiología , Infecciones por Herpesviridae/patología , Inmunoglobulina G/inmunología , Inmunoglobulina M/inmunología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Síndrome de Inmunodeficiencia Adquirida del Murino/inmunología , Muromegalovirus/aislamiento & purificación , Retina/patologíaRESUMEN
Macrophage inhibitory factor-A3 (MIF-A3), a fraction derived from Mycobacterium avium serovar 2 inhibited candidacidal activity in macrophages from C57BL/6, C57BL/10, C3H/HeJ and A/J strains of mice. Inhibition of candidacidal activity was demonstrated at MIF-A3 concentrations ranging from 100-400 micrograms/ml in macrophages without additional stimulators (exception C3H/HeJ macrophages) and in macrophages additionally stimulated with 200 U/ml interferon-gamma, 100 ng/ml phorbol myristate acetate and 0.4 ng/ml E. coli lipopolysaccharide from all mouse strains tested. The decreased candidacidal effect produced by MIF-A3 was dose-dependent and appeared greatest in macrophages treated with phorbol myristate acetate and lipopolysaccharide. This effect was neutralized by the addition of goat anti-MIF-A3 antiserum. Macrophages from the Bcgs mouse strains (C57BL/6 and C57Bl/100 were more sensitive to the effect(s) of MIF-A3 than macrophages from the Bcgr mouse strains (C3H/HeJ and A/J).
Asunto(s)
Candida albicans , Glucolípidos/farmacología , Glicopéptidos/farmacología , Macrófagos Peritoneales/fisiología , Mycobacterium avium/química , Análisis de Varianza , Animales , Anticuerpos , Células Cultivadas , Depuradores de Radicales Libres/farmacología , Glucolípidos/inmunología , Glucolípidos/aislamiento & purificación , Glicopéptidos/inmunología , Glicopéptidos/aislamiento & purificación , Lipopolisacáridos/farmacología , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/microbiología , Ratones , Ratones Endogámicos A , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Mycobacterium avium subsp. paratuberculosis , Fagocitosis/efectos de los fármacos , Especificidad de la Especie , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
The present studies were undertaken to examine whether concurrent MCMV infection during allogeneic bone marrow transplantation (BMT) could alter the developing donor-host immune interactions and affect the overall outcome of the transplant. In order to determine the effect of MCMV on antihost activity arising following an allogeneic BMT, specific donor antihost cytotoxicity was examined. The results demonstrated that concurrent virus infection in mice receiving a BMT from donors either H2-matched and non-MHC-mismatched or mismatched at both MHC and non-MHC transplantation loci, augmented antihost cytotoxic activity mediated by CD8+ T cells assayed directly from the recipient's spleen 10-14 days posttransplant. Notably, allogenic BMT recipients receiving either lethal or nonlethal numbers of donor T cells and inoculated with MCMV exhibited more rapid and profound weight loss compared with uninfected allogeneic and syngeneic BMT recipients. Concurrent virus presence also resulted in a markedly increased incidence of mortality in allogeneic BMT recipients of nonlethal numbers of T cells. We conclude from these findings that when virus is present early after allogeneic BMT, the resulting interactions can potentiate T cell-mediated donor-antirecipient--i.e., graft vs. host-reactivity. In total, the results support the notion that pathogens could complicate allogeneic BMT by contributing to the development of graft vs. host disease.
Asunto(s)
Trasplante de Médula Ósea/inmunología , Infecciones por Herpesviridae/inmunología , Muromegalovirus/inmunología , Animales , Linfocitos T CD4-Positivos/inmunología , Citotoxicidad Inmunológica , Femenino , Enfermedad Injerto contra Huésped/inmunología , Enfermedad Injerto contra Huésped/virología , Activación de Linfocitos/inmunología , Ratones , Ratones Endogámicos BALB C , Sensibilidad y Especificidad , Resultado del TratamientoRESUMEN
Targeted oncogenesis in transgenic mice has unexpectedly produced predictable tissue-specific tumors. We previously showed that hybrid gene constructs of the human fetal G gamma- or mouse embryonic beta h1-globin promoter linked to the viral simian virus 40 T antigen (G gamma/T and beta h1/T) expressed appropriately in embryonic erythroid tissue, with some unexpected expression elsewhere. Tumors arising in the G gamma/T and beta h1/T transgenic mice were identified by histology, electron microscopy, cell culture, and RNase protection analyses. In one G gamma/T transgenic line, males developed prostate tumors that showed mixed neuroendocrine and epithelial cell features, whereas females developed adrenocortical tumors. In several other G gamma/T lines, brown adipose tumors, or hibernomas, developed in the subcutaneous interscapular neck and shoulder area, as well as internally in the periadrenal and pericardial areas. Little or no expression of T antigen was detected in adult animals before visible tumor formation. In contrast, beta h1/T transgenic mice developed only choroid plexus tumors. Transient transfection assays in prostate and adrenocortical tumor-derived cell lines showed that the G gamma-globin promoter is 7-to 10-fold more active than the beta h1-globin promoter. Activity of 5' G gamma-globin promoter-deletion DNA plasmids was analyzed by transient transfection in a variety of human prostate cancer cell lines. The G gamma-globin promoter region between -140 and -201 also showed high activity in the androgen-independent human prostate cancer cell lines DU-145 and PPC-1, but low activity in the androgen-responsive human prostate cell line LNCaP. We conclude that tumor formation in the G gamma/T transgenic lines apparently results from cryptic positive DNA cis elements active in prostate and adrenocortical cells. Because G gamma-globin promoter activity is highest in embryonic tissue, tumors in adult transgenic mice may result from expression of T antigen in embryonic prostate, adrenal glands, and brown adipose tissue.
Asunto(s)
Neoplasias de la Corteza Suprarrenal/genética , Antígenos Virales de Tumores/genética , Hemoglobina Fetal/genética , Lipoma/genética , Ratones Transgénicos/genética , Neoplasias de la Próstata/genética , Neoplasias de la Corteza Suprarrenal/patología , Andrógenos/fisiología , Animales , ADN de Neoplasias/metabolismo , Femenino , Lipoma/patología , Masculino , Ratones , Regiones Promotoras Genéticas , Neoplasias de la Próstata/patología , ARN/metabolismo , Células Tumorales CultivadasRESUMEN
Although various methods of immunosuppression have been used to enhance susceptibility of mice to murine cytomegalovirus (MCMV) retinitis, none reproduce the unique complexity of immune deficiency experienced by patients during the progression of AIDS. C57BL/6 mice are susceptible to a retrovirus-induced murine acquired immune deficiency syndrome (MAIDS), characterized by progressive immune dysfunction which shares many features with AIDS. We therefore evaluated the frequency and severity of MCMV retinitis in C57BL/6 mice with MAIDS. Following subretinal inoculation of MCMV, nearly 90% of mice with MAIDS developed a necrotizing retinitis 8 to 10 days postinfection, whereas retinitis was observed in only 8% of age-matched immunocompetent control mice. Histopathologic analysis of the retinitis that developed in mice with MAIDS revealed features similar to those found in AIDS-related CMV retinitis. Eyes from MAIDS animals also contained on average higher amounts of infectious virus when compared with eyes from control animals. We conclude that retrovirus-induced immunosuppression increases susceptibility of C57BL/6 mice to MCMV retinitis.