RESUMEN

VP22, encoded by the UL49 gene of Marek's disease virus (MDV), is indispensable for virus cell-to-cell spreading. We show herein that MDV UL49 can be functionally replaced with avian and human viral orthologs. Replacement of MDV VP22 with that of avian gallid herpesvirus 3 or herpesvirus of turkey, whose residue identity with MDV is close to 60%, resulted in 73 and 131% changes in viral spreading, respectively. In contrast, VP22 replacement with human herpes simplex virus type 1 resulted in 14% plaque formation. Therefore, heterologous avian and human VP22 proteins share sufficient structural homology to support MDV cell-to-cell spreading, albeit with different efficiencies.

Asunto(s)

Alphaherpesvirinae/clasificación , Alphaherpesvirinae/fisiología , Herpesvirus Gallináceo 2/fisiología , Homología de Secuencia de Aminoácido , Proteínas Virales , Alphaherpesvirinae/genética , Alphaherpesvirinae/metabolismo , Animales , Aves/virología , Células Cultivadas , Embrión de Pollo , Prueba de Complementación Genética , Herpesvirus Humano 1/genética , Herpesvirus Humano 1/metabolismo , Herpesvirus Gallináceo 2/genética , Herpesvirus Gallináceo 2/metabolismo , Herpesvirus Gallináceo 3/genética , Herpesvirus Gallináceo 3/metabolismo , Humanos , Mardivirus/genética , Mardivirus/metabolismo , Piel/citología , Proteínas Virales/genética , Proteínas Virales/metabolismo