RESUMEN
The currently available options for tooth-loss are prostheses, implants, or surgery (auto-transplantation). They all have their limitations. The emergence of tissue engineering, 15 years ago, was made possible by a better knowledge of the various stages of dental development, and the mastery of stem cell differentiation. It opened a new alternative approach for tooth regeneration. Even if animal experiments have demonstrated that it was possible to obtain a biological tooth from stem cells, two major issues remain to be discussed. Is it possible to use induced pluripotent stem cells instead of embryonic stem cells, which raise an ethical problem? Is it possible to reproduce a dental crown with an adapted shape and colour? Or should we consider the simpler creation of a biological root secondarily covered by a ceramic prosthesis? Our study mentions the main landmarks and the key cells involved in the embryological development of the tooth, establishes a mapping and a list of the various types of stem cells. It details the various methods used to create a biological implant.
Asunto(s)
Pulpa Dental/citología , Odontogénesis , Regeneración , Células Madre , Ingeniería de Tejidos , Diente/embriología , Animales , Técnicas de Cultivo de Célula , Costos y Análisis de Costo , Humanos , Células Madre Pluripotentes Inducidas , Células Madre Mesenquimatosas , Odontogénesis/genética , Diente/crecimiento & desarrollo , Corona del Diente/crecimiento & desarrollo , Germen Dentario/citología , Raíz del Diente/crecimiento & desarrolloRESUMEN
Embryonic dental cells were used to check a series of criteria to be achieved for tooth engineering. Implantation of cultured cell-cell re-associations led to crown morphogenesis, epithelial histogenesis, organ vascularization, and root and periodontium development. The present work aimed to investigate the organization of predentin/dentin, enamel, and cementum which formed and mineralized after implantation. These implants were processed for histology, transmission electron microscopy, x-ray microanalysis, and electron diffraction. After two weeks of implantation, the re-associations showed gradients of differentiating odontoblasts. There were ciliated, polarized, and extended cell processes in predentin/dentin. Ameloblasts became functional. Enamel crystals showed a typical oriented arrangement in the inner and outer enamel. In the developing root, odontoblasts differentiated, cementogenesis occurred, and periodontal ligament fibroblasts interacted with the root surface and newly formed bone. The implantation of cultured dental cell re-associations allows for reproduction of complete functional differentiation at the cell, matrix, and mineral levels.
Asunto(s)
Diferenciación Celular , Células Madre Embrionarias/citología , Matriz Extracelular/ultraestructura , Trasplante de Células Madre , Ingeniería de Tejidos , Corona del Diente/crecimiento & desarrollo , Raíz del Diente/crecimiento & desarrollo , Ameloblastos/citología , Ameloblastos/ultraestructura , Amelogénesis , Animales , Polaridad Celular , Células Cultivadas , Cementogénesis , Cristalización , Dentinogénesis , Microanálisis por Sonda Electrónica , Células Madre Embrionarias/trasplante , Órgano del Esmalte/citología , Ratones , Ratones Endogámicos ICR , Microscopía Electrónica de Transmisión , Morfogénesis , Neovascularización Fisiológica , Odontoblastos/citología , Odontoblastos/ultraestructura , Ligamento Periodontal/crecimiento & desarrolloRESUMEN
A primary cilium, a sensory organelle present in almost every vertebrate cell, is regularly described in odontoblasts, projecting from the surfaces of the cells. Based on the hypothesis that the primary cilium is crucial both for dentin formation and possibly in tooth pain transmission, we have investigated the expression and localization of the main cilium components and involvement of the OFD1 gene in tooth morphogenesis. Odontoblasts in vitro express tubulin, inversin, rootletin, OFD1, BBS4, BBS6, ALMS1, KIF3A, PC1, and PC2. In vivo, cilia are aligned parallel to the dentin walls, with the top part oriented toward the pulp core. Close relationships between cilium and nerve fibers are evidenced. Calcium channels are concentrated in the vicinity of the basal body. Analysis of these data suggests a putative role of cilia in sensing the microenvironment, probably related to dentin secretion. This hypothesis is enhanced by the huge defects observed on molars from Ofd1 knockout mice, showing undifferentiated dentin-forming cells.
Asunto(s)
Cilios/fisiología , Proteínas de Choque Térmico/fisiología , Diente Molar/crecimiento & desarrollo , Odontoblastos/fisiología , Odontogénesis/fisiología , Adolescente , Animales , Canales de Calcio/análisis , Canales de Calcio/ultraestructura , Proteínas de Ciclo Celular , Movimiento Celular , Células Cultivadas , Proteínas del Citoesqueleto/análisis , Pulpa Dental/ultraestructura , Dentina/ultraestructura , Chaperoninas del Grupo II , Proteínas de Choque Térmico/análisis , Humanos , Cinesinas/análisis , Ratones , Ratones Noqueados , Proteínas Asociadas a Microtúbulos , Diente Molar/ultraestructura , Chaperonas Moleculares/análisis , Morfogénesis/fisiología , Fibras Nerviosas/ultraestructura , Proteínas/análisis , Canales Catiónicos TRPP/análisis , Factores de Transcripción/análisis , Tubulina (Proteína)/análisisRESUMEN
Previously, we established a subtractive cDNA library enriched in odontoblast-specific genes and hypothesized that new, previously unidentified, markers would be present, associated with the odontoblast phenotype. In this paper, we report the first characterization of a new gene we have named HUGO, and its associated deduced protein sequence. This gene expression is under the control of two alternative promoters, resulting in the synthesis of two proteins, one of which, HUGO2, is included in the other, HUGO1. HUGO proteins are mainly composed of a proline-rich region at the N-terminus, 8 type III-fibronectin modules, and a transmembranous helix at the C-terminus. In odontoblasts, the proteins are located in Golgi vesicles. However, they display a broader expression pattern, since they are also expressed by nerve fibers in the dental pulp and other tissues (e.g., trachea, brain, kidney), as demonstrated by immunohistochemistry and qPCR, respectively. Their location in odontoblasts suggests a role in collagen and glycosaminoglycan synthesis.
Asunto(s)
Fibronectinas/genética , Odontoblastos/metabolismo , Animales , Northern Blotting , Western Blotting , Células COS , Células Cultivadas , Chlorocebus aethiops , Cromosomas Humanos Par 13/genética , Secuencia Conservada/genética , Pulpa Dental/citología , Pulpa Dental/inervación , Exones/genética , Citometría de Flujo , Regulación de la Expresión Génica/genética , Aparato de Golgi/metabolismo , Humanos , Inmunohistoquímica , Intrones/genética , Proteínas de Neoplasias/genética , Sistemas de Lectura Abierta/genética , Reacción en Cadena de la Polimerasa , Regiones Promotoras Genéticas/genética , Isoformas de Proteínas/genética , Análisis de Secuencia de ADN , Homología Estructural de ProteínaRESUMEN
Our knowledge of the gene coding for amelogenin, the major enamel protein, is mainly based on mammalian sequences. Only two sequences are available in reptiles. To know whether the snake sequence is representative of the amelogenin condition in squamates, we have studied amelogenin in two scincid lizards. Lizard amelogenin possesses numerous conserved residues in the N- and C-terminal regions, but its central region is highly variable, even when compared with the snake sequence. This rapid evolution rate indicates that a single squamate sequence was not representative, and that comparative studies of reptilian amelogenins might be useful to detect the residues which are really important for amelogenin structure and function. Reptilian and mammalian enamel structure is roughly similar, but no data support amelogenin being similarly expressed during amelogenesis. By performing in situ hybridization using a specific probe, we showed that lizard ameloblasts express amelogenin as described during mammalian amelogenesis. However, we have not found amelogenin transcripts in odontoblasts. This indicates that full-length amelogenin is specific to enamel matrix, at least in this lizard.
Asunto(s)
Amelogénesis/genética , Proteínas del Esmalte Dental/genética , Lagartos/genética , Proteínas de Reptiles/genética , Ameloblastos/metabolismo , Amelogenina , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Proteínas del Esmalte Dental/biosíntesis , Proteínas del Esmalte Dental/química , Expresión Génica , Hibridación in Situ , Datos de Secuencia Molecular , Odontoblastos/metabolismo , Análisis de Secuencia de ADNRESUMEN
Integrin alphabeta heterodimers mediate adhesion to the extracellular matrix and at cell-cell contacts and initiate intracellular signalling cascades in response to a variety of inductive factors. Apart from the expression of alphavbeta3 that we have previously reported, little is known about the expression of integrins in odontoblasts. Here, we investigated the expression of alphav-binding beta integrin subunits in healthy human dental pulp in vivo and in odontoblasts differentiated in vitro. Reverse transcription/polymerase chain reaction analysis revealed the expression of alphav, beta1, beta5 and beta8 integrin mRNA, but not beta6, in whole pulp cells. Flow cytometry showed that the alphav and beta1 subunits were the most intensely expressed. Immunohistochemistry demonstrated that the beta1 subunit was localised in newly differentiated odontoblasts in the root and in mature odontoblasts in the crown, including their intradentinal cell processes. The alphav chain was predominantly expressed by mature odontoblasts and alphavbeta5 was only observed in mature odontoblasts. In vitro differentiated odontoblasts expressed genes for alphav, beta1 and beta5, but not for beta6 and beta8. A comparison of integrin profiles between cultured pulp cells and in vitro differentiated odontoblasts revealed that odontoblast maturation was characterised by a significant increase in the expression of alphav and beta1 subunits and alphavbeta5 integrin. The beta8 subunit was detected in nerve cells only. Histological analysis of teeth from alphav knockout mice showed no obvious structural modification in the odontoblast layer. Thus, human mature odontoblasts express alphavbeta3, alphavbeta5 and perhaps alphavbeta1 integrins, with the possible presence of alpha-beta1 pairs. The roles that these molecules play in the exchange of information throughout the odontoblast layer remain to be determined.
Asunto(s)
Pulpa Dental/citología , Integrina alfaV/metabolismo , Odontoblastos/citología , Adolescente , Animales , Diferenciación Celular , Células Cultivadas , Pulpa Dental/metabolismo , Humanos , Inmunohistoquímica , Ratones , Ratones Noqueados , Odontoblastos/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Integrins are heterodimeric transmembrane receptors which promote cell adhesion, thus contributing to the maintenance of tissue organization in both normal and pathological conditions. To characterize the way odontoblasts may interact with other cells and the extracellular matrix in human teeth, we studied expression of alpha v beta 3 integrin, a putative receptor for osteoadherin. We showed that alpha v beta 3 integrin expression was restricted to odontoblasts, blood vessels, and small rounded cells in sound and carious pulp. Odontoblast staining intensity increased from the apical to the cusp region. Osteoadherin staining was strong in the whole odontoblast layer (with a slight decrease in the cusp region) and in predentin. Odontoblasts differentiating in vitro were stained with the anti-alpha v beta 3 integrin antibody, first at the level of intercellular contacts, then throughout the cell membrane. These results suggest that the alpha v beta 3 integrin could play a role in interodontoblast adhesion and odontoblast binding to the surrounding predentin/dentin/pulp matrix, possibly through osteoadherin.
Asunto(s)
Pulpa Dental/metabolismo , Proteínas de la Matriz Extracelular/metabolismo , Integrina alfaV/metabolismo , Integrina alfaVbeta3/metabolismo , Integrina beta3/metabolismo , Odontoblastos/metabolismo , Proteoglicanos/metabolismo , Adolescente , Adhesión Celular/fisiología , Células Cultivadas , Pulpa Dental/citología , Proteínas de la Matriz Extracelular/genética , Humanos , Inmunohistoquímica , Hibridación in Situ , Integrina alfaV/genética , Integrina alfaVbeta3/genética , Integrina beta3/genética , Tercer Molar/citología , Subunidades de Proteína/genética , Subunidades de Proteína/metabolismo , Proteoglicanos/genética , ARN Mensajero/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Distribución TisularRESUMEN
During tooth development, odontoblasts are the cells that form dentin and possibly mediate early stages of sensory processing in teeth. It is suggested that ion channels assist in these events. Indeed, mechanosensitive potassium currents, transducing mechanical stimuli into electrical cell signals, have been previously recorded in the human odontoblast cell membrane. Here, we show by RT-PCR that the mechanosensitive potassium channel TREK-1 (a member of the two-pore-domain potassium channel family) is overexpressed in these cultured cells compared with pulp cells in vitro. In situ hybridization showed that transcripts are detected in the odontoblast layer in vivo. The use of antibodies shows that TREK-1 is strongly expressed in the membrane of coronal odontoblasts and absent in the root. This distribution is related to the spatial distribution of nerve endings identified by labeling of the low-affinity nerve growth factor (NGF) receptor (p75(NTR)). These results demonstrate the expression of TREK-1 in human odontoblasts in vitro and in vivo.
Asunto(s)
Odontoblastos/metabolismo , Canales de Potasio de Dominio Poro en Tándem , Canales de Potasio/biosíntesis , Membrana Celular/metabolismo , Células Cultivadas , Pulpa Dental/citología , Expresión Génica , Humanos , Inmunohistoquímica , Hibridación in Situ , ARN Mensajero/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Corona del Diente/citologíaRESUMEN
The membrane cytoskeleton is increasingly considered as both an anchor and a functional modulator for ion channels. The cytoskeletal disruptions that occur in the absence of dystrophin led us to investigate the voltage-gated sodium channel (SkM1) content in the extensor digitorum longus (EDL) muscle of the dystrophin-deficient mdx mouse. Levels of SkM1 mRNA were determined by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). A C-terminal portion of the mouse-specific SkM1 alpha-subunit cDNA (mScn4a) was identified first. SkM1 mRNA levels were as abundant in mdx as in normal muscle, thus suggesting that the transcriptional rate of SkM1 remains unchanged in mdx muscle. However, SkMI density in the extrajunctional sarcolemma was shown to be significantly reduced in mdx muscle, using confocal immunofluorescence image analysis. This decrease was found to be associated with a reduction in the number of SkM1-rich fast-twitch IIb fibres in mdx muscle. In addition, lowered SkM1 sarcolemmal labelling was found in all mdx fibres regardless of their metabolic type. These results suggest the existence of a perturbation of SkM1 anchorage to the plasma membrane. Such an alteration is likely to be related to the 50% decrease in mdx muscle of the dystrophin-associated syntrophins, which are presumed to be involved in SkM1 anchorage. However, the moderate reduction in SkM1 density (-12.7%) observed in mdx muscle argues in favour of a non-exclusive role of syntrophins in SkM1 anchorage and suggests that other membrane-associated proteins are probably also involved.
Asunto(s)
Distrofina/genética , Músculo Esquelético/fisiología , Distrofia Muscular Animal/metabolismo , Canales de Sodio/genética , Canales de Sodio/metabolismo , Animales , Secuencia de Bases , Secuencia de Consenso , Expresión Génica , Inmunohistoquímica , Activación del Canal Iónico/fisiología , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos mdx , Datos de Secuencia Molecular , Fibras Musculares de Contracción Rápida/química , Fibras Musculares de Contracción Rápida/metabolismo , Fibras Musculares de Contracción Rápida/patología , Fibras Musculares de Contracción Lenta/química , Fibras Musculares de Contracción Lenta/metabolismo , Fibras Musculares de Contracción Lenta/patología , Músculo Esquelético/química , Músculo Esquelético/patología , Distrofia Muscular Animal/genética , Distrofia Muscular Animal/patología , ARN Mensajero/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sarcolema/química , Sarcolema/metabolismo , Canales de Sodio/análisisRESUMEN
The odontoblast phenotype has been mainly approached by the biochemical characterization of dentin matrix proteins and by extrapolation of the knowledge of bone cell biology, since dentin and bone share many similarities. In fact, direct investigations of the odontoblast phenotype have been hindered by the limited number of cells within the dental pulp and the difficulty in microdissection and isolation of a pure population of these cells. To overcome these obstacles, we previously developed a cell-culture system that promotes differentiation of human dental pulp cells into odontoblasts. This material now permits the study of odontoblasts through molecular biology techniques. Therefore, we constructed a cDNA library enriched for odontoblast-specific genes using the suppression subtractive hybridization technique (SSH). This library led us to identify new genes expressed by odontoblasts. In this paper, we will focus on some genes implied in various functions associated with odontoblast differentiation, such as cell polarization (MAP1B), dentin mineralization (PHEX, osteoadherin), and relationships between odontoblasts and nerve cells (reelin). Another important fact is that about 40% of the cDNA were unknown genes. Therefore, one can speculate that some of them will be odontoblast-specific, since, until now, only one gene (DSPP) presents this characteristic.
Asunto(s)
Dentinogénesis/genética , Odontoblastos/metabolismo , Moléculas de Adhesión Celular Neuronal/genética , Técnicas de Cultivo de Célula , Diferenciación Celular/genética , Polaridad Celular/genética , Pulpa Dental/citología , Proteínas de la Matriz Extracelular/genética , Biblioteca de Genes , Humanos , Hibridación Genética/genética , Microcuerpos/genética , Proteínas Asociadas a Microtúbulos/genética , Biología Molecular , Proteínas del Tejido Nervioso/genética , Endopeptidasa Neutra Reguladora de Fosfato PHEX , Fenotipo , Fosfoproteínas/genética , Precursores de Proteínas/genética , Proteínas/genética , Proteoglicanos/genética , Proteína Reelina , Serina Endopeptidasas , Sialoglicoproteínas , Supresión Genética/genética , Calcificación de Dientes/genéticaRESUMEN
Pulp tissue responds to dentin damage by laying down a tertiary dentin matrix (reactionary or reparative) beneath the site of injury. Reactionary dentin is secreted by surviving odontoblasts in response to environmental stimuli, leading to an increase in metabolic activities of the cells. The inductive molecules that determine the success of the pulp healing may be released from the damaged dentin as well as from the pulp tissue subjacent to the injury. This paper will schematically consider two major growth factors probably implicated in the control of odontoblast activity: TGF beta-1 released from demineralized dentin and NGF from pulp. To analyze their role with an in vitro system that mimics the in vivo situation, we have used thick-sliced teeth cultured as described previously. The supply of factors was accomplished by means of a small tube glued onto the dentin. The tube was filled with TGF beta-1 (20 ng/mL) or NGF (50 ng/mL), and slices were cultured for 4 or 7 days. Results showed that TGF beta-1 binding sites are strongly detected on odontoblasts in the factor-rich zone. A strong expression of alpha 1(I) collagen transcripts was also detected. In the NGF-rich environment, p75NTR was re-expressed on odontoblasts and the transcription factor NF-kappa B activated. Modifications in the odontoblast morphology were observed with an atypical extension of the cell processes filled with actin filaments. These results suggest that odontoblasts respond to influences from both dentin and pulp tissue during pulp repair.
Asunto(s)
Colágeno Tipo I , Dentina Secundaria/fisiología , Dentina/lesiones , Odontoblastos/fisiología , Citoesqueleto de Actina/ultraestructura , Actinas/análisis , Colágeno/análisis , Cadena alfa 1 del Colágeno Tipo I , Técnicas de Cultivo , Pulpa Dental/metabolismo , Pulpa Dental/fisiopatología , Dentina/fisiopatología , Dentina/ultraestructura , Dentina Secundaria/ultraestructura , Dentinogénesis/fisiología , Humanos , Biología Molecular , FN-kappa B/análisis , Factor de Crecimiento Nervioso/fisiología , Odontoblastos/ultraestructura , Receptor de Factor de Crecimiento Nervioso , Receptores de Factor de Crecimiento Nervioso/análisis , Receptores de Factores de Crecimiento Transformadores beta/análisis , Desmineralización Dental/fisiopatología , Factor de Crecimiento Transformador beta/fisiología , Factor de Crecimiento Transformador beta1 , Cicatrización de Heridas/fisiologíaRESUMEN
Members of the TGF-beta family of growth factors are important in modulation of odontoblast secretory activity during dental tissue repair. Odontoblast expression of TGF-beta isoforms during development leads to their sequestration within the dentin matrix, from where they may be released during carious injury and participate in reparative processes. Two receptors, implicated in TGF-beta-mediated cell signaling, have been identified immunohistochemically in both odontoblasts and pulpal cells of healthy and carious human molar teeth. This study aimed to characterize the expression of the TGF-beta receptors I and II in sound and carious teeth by means of in situ hybridization, to help our understanding of the response of these cells to TGF-beta stimulation. Sound and carious human third molar teeth were routinely processed immediately following extraction, and 10-microns paraffin-embedded sections prepared. These sections were hybridized with 32P-labeled probes to TGF-beta receptors I and II, and the subsequent signal was detected by autoradiography. mRNA for both receptors I and II was mainly detected within the odontoblasts and nerve-associated cells of healthy tissues, with expression at lower levels seen within the subodontoblast and pulp core cells. The expression in odontoblasts was higher for TGF-beta receptor I than for receptor II. Expression of both receptors was more homogenous in all pulp cells within carious teeth, because of an increase of signal within the underlying pulp cell population, including blood-vessel-associated cells. We conclude that the TGF-beta receptors I and II were expressed in odontoblasts and pulp cells, and that subtle variations in the levels of their expression could be involved in the tissue response to injury.
Asunto(s)
Receptores de Activinas Tipo I/análisis , Pulpa Dental/patología , Proteínas Serina-Treonina Quinasas/análisis , Receptores de Factores de Crecimiento Transformadores beta/análisis , Factor de Crecimiento Transformador beta/análisis , Receptores de Activinas Tipo I/genética , Autorradiografía , Caries Dental/metabolismo , Caries Dental/patología , Pulpa Dental/metabolismo , Dentina/metabolismo , Dentina/patología , Expresión Génica , Humanos , Hibridación in Situ , Tercer Molar , Odontoblastos/metabolismo , Odontoblastos/patología , Radioisótopos de Fósforo , Isoformas de Proteínas/metabolismo , Proteínas Serina-Treonina Quinasas/genética , ARN Mensajero/análisis , Radiofármacos , Receptor Tipo I de Factor de Crecimiento Transformador beta , Receptor Tipo II de Factor de Crecimiento Transformador beta , Receptores de Factores de Crecimiento Transformadores beta/genética , Transducción de Señal/fisiología , Factor de Crecimiento Transformador beta/genética , Factor de Crecimiento Transformador beta/metabolismo , Cicatrización de HeridasRESUMEN
Transforming growth factor-beta1 (TGF beta1) is a potent modulator of tissue repair in various tissues. To analyze its role during human dental repair, we used thick-sliced teeth cultured as described previously (Magloire et al., 1996). The supply of TGF beta1 to the pulp tissue was accomplished by means of a small tube glued onto the dentin. We show that this device allowed the growth factor to diffuse locally through dentinal tubules and to bind to the cells present in the coronal pulp opposite the TGF beta1-delivery tube. The tube was filled with 20 ng/mL TGF beta1, and slices were cultured for 4 days. Results show a preferential accumulation of cells in the odontoblastic and subodontoblastic layers in the vicinity of the tube. Cell proliferation increased in the subodontoblastic layer and in the underlying pulp, and BrdU-positive cells were abundant around the blood vessels. TGF beta1 induced type I collagen production by the odontoblastic/subodontoblastic/pulp cells in the stimulated zone, as demonstrated by in situ hybridization. These results suggest that TGF beta1 could be directly involved in the regulation of cell proliferation, migration, and extracellular matrix production in the human dental pulp and eventually in the repair process occurring after tooth injury.
Asunto(s)
Pulpa Dental/efectos de los fármacos , Factor de Crecimiento Transformador beta/farmacología , Adolescente , Adulto , Autorradiografía , División Celular , Inhibición de Migración Celular , Colágeno/biosíntesis , Colágeno/efectos de los fármacos , Técnicas de Cultivo , Pulpa Dental/citología , Pulpa Dental/metabolismo , Humanos , Hibridación in Situ/métodos , Odontoblastos/citología , Odontoblastos/efectos de los fármacos , Odontoblastos/metabolismo , Factores de TiempoRESUMEN
Odontoblasts are highly specialized cells aligned at the edge of the dental pulp. As a step towards understanding the complex mechanisms underlying their terminal differentiation, the gene expression pattern was examined in human cultured odontoblast cells. Suppression substractive hybridization (SSH) was used to establish a substracted cDNA library specific for human odontoblasts. For this purpose, cDNAs from human cultured fibroblastic pulp cells were substracted to cDNA from human cultured odontoblasts. The nucleotide sequence of 154 substracted cDNA clones was determined. We identified 130 preferentially expressed gene fragments in odontoblasts as compared with the fibroblastic pulp cells. Ten of them were already identified in odontoblasts such as DSPP, BSP, enamelysin and Col1A1. We confirmed their overexpression by RT-PCR on the cultured cells and in vivo by in situ hybridization on human molars. Another 64 clones corresponded to known genes. Among them, two clones were of particular interest: reelin, which was first detected in the brain and osteoadherin, which was first located in bone. Fifty-six clones were unknown genes even though 82% matched expressed sequence tags or genomic clones. A reverse Northern dot blot showed that 96% of them were overexpressed at different rates in cultured odontoblasts. These latest results indicate that there are still unknown genes that are associated with the control of the odontoblast phenotype. Thus, cloning of odontoblast differentiation-associated genes not only opens up new methods of elucidating the normal development but also the recruitment of odontoblasts when required to initiate repair of dentin.
Asunto(s)
Biblioteca de Genes , Odontoblastos/metabolismo , Secuencia de Bases , Diferenciación Celular/genética , Células Cultivadas , Cartilla de ADN/genética , ADN Complementario/genética , Expresión Génica , Humanos , Odontoblastos/citología , Odontogénesis/genética , Reacción en Cadena de la Polimerasa/métodos , Proteína ReelinaRESUMEN
Because the extracellular matrices of dentin and bone are composed mainly of type I collagen, their characteristics are determined by the nature of noncollagenous proteins (NCPs). Among these NCPs, some proteoglycans (PGs) belong to the small leucine-rich proteoglycans (SLRPs). Recently, osteoadherin (OSAD) has been described as a new member of this family, that is expressed by mature bovine osteoblasts. Here, we report the expression of OSAD messenger RNA (mRNA) in human dental tissues and during the development of rat molars, using in situ hybridization. For this purpose, we constructed a probe for OSAD mRNA transcripts from human odontoblast cells cultured in vitro. Our results indicate that the mature human odontoblasts overexpress the OSAD gene as compared with cells present in the pulp core. In rat developing molars, mRNA transcripts were first detected in alveolar bone in 19-day-old embryos. At the same age, no signal was detected in any cell of the first molar. In more mature teeth (newborn and 2-day-old rats), OSAD expression starts in the polarized odontoblasts and increases in the secretory and mature odontoblasts, respectively. Interestingly, a similar pattern of expression was observed in the ameloblast layer responsible for the deposition of enamel mineralized matrix. Together, these results lead us to speculate that OSAD may be implicated in biomineralization processes.
Asunto(s)
Pulpa Dental/química , Pulpa Dental/fisiología , Proteínas de la Matriz Extracelular/genética , Proteoglicanos/genética , Diente/química , Diente/fisiología , Factores de Edad , Animales , Animales Recién Nacidos , Calcificación Fisiológica/fisiología , Bovinos , Pulpa Dental/crecimiento & desarrollo , Proteínas de la Matriz Extracelular/análisis , Proteínas de la Matriz Extracelular/química , Femenino , Feto/química , Feto/fisiología , Regulación del Desarrollo de la Expresión Génica , Humanos , Hibridación in Situ , Leucina/química , Ratones , Datos de Secuencia Molecular , Proteoglicanos/análisis , Proteoglicanos/química , ARN Mensajero/análisis , Ratas , Ratas Sprague-Dawley , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Aminoácido , Diente/crecimiento & desarrolloRESUMEN
Odontoblasts form a layer of cells responsible for the dentin formation and possibly mediate early stages of sensory processing in teeth. Several classes of ion channels have previously been identified in the odontoblast or pulp cell membrane, and it is suspected that these channels assist in these events. This study was carried out to characterize the K(Ca) channels on odontoblasts fully differentiated in vitro using the patch clamp technique and to investigate the HSLO gene expression encoding the alpha-subunit of these channels on odontoblasts in vivo. In inside-out patches, K(Ca) channels were identified on the basis of their K(+) selectivity, conductance, voltage, and Ca(2+) dependence. In cell-attached patches, these channels were found to be activated by application of a negative pressure as well as an osmotic shock. By reverse transcription-polymerase chain reaction, a probe complementary to K(Ca) alpha-subunit mRNA was constructed and used for in situ hybridization on human dental pulp samples. Transcripts were expressed in the odontoblast layer. The use of antibodies showed that the K(Ca) channels were preferentially detected at the apical pole of the odontoblasts. These channels could be involved in mineralization processes. Their mechanosensitivity suggests that the fluid displacement within dentinal tubules could be transduced into electrical cell signals.
Asunto(s)
Calcio/metabolismo , Expresión Génica , Odontoblastos/metabolismo , Canales de Potasio Calcio-Activados , Canales de Potasio/química , Canales de Potasio/metabolismo , Adolescente , Diferenciación Celular , Membrana Celular/metabolismo , Células Cultivadas , ADN Complementario/metabolismo , Pulpa Dental/citología , Pulpa Dental/crecimiento & desarrollo , Pulpa Dental/metabolismo , Humanos , Inmunohistoquímica , Hibridación in Situ , Subunidades alfa de los Canales de Potasio de Gran Conductancia Activados por Calcio , Canales de Potasio de Gran Conductancia Activados por el Calcio , Neuronas/metabolismo , Odontoblastos/citología , Técnicas de Placa-Clamp , Canales de Potasio/biosíntesis , Presión , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Estrés MecánicoRESUMEN
In order to elucidate the mechanisms involved in human dentin formation, we developed a cell culture system to promote differentiation of dental pulp cells into odontoblasts. Explants from human teeth were cultured in Eagle's basal medium supplemented with 10% or 15% fetal calf serum, with or without beta-glycerophosphate (beta GP). Addition of beta GP to the culture medium induced odontoblast features in the cultured pulp cells. Cells polarized and some of them exhibited a typical cellular extension. In some cases, cells aligned with their processes oriented in the same direction and developed junctional complexes similar to the terminal web linking odontoblasts in vivo. Fine structural analyses showed the presence of typical intracellular organelles of the odontoblast body, whereas the process contained only cytoskeleton elements and secretory vesicles. Polarized cells deposited onto the plastic dishes an abundant and organized type I collagen-rich matrix with areas of mineralization appearing thereafter. X-ray microanalysis showed the presence of calcium and phosphorus and the electron diffraction pattern confirmed the apatitic crystal structure of the mineral. High expression of alpha 1 (1) collagen mRNAs was detected in all polarized cells whereas dentin sialoprotein gene was mainly expressed in mineralizing areas. This cell culture system allowed for the differentiation of pulp cells into odontoblasts, at both the morphological and functional level. Moreover, these cells presented a spatial organization similar to the odontoblastic layer.
Asunto(s)
Diferenciación Celular , Pulpa Dental/citología , Odontoblastos/citología , Adolescente , Diferenciación Celular/efectos de los fármacos , Polaridad Celular , Medios de Cultivo , Sondas de ADN , Pulpa Dental/efectos de los fármacos , Microanálisis por Sonda Electrónica , Matriz Extracelular/ultraestructura , Glicerofosfatos/farmacología , Humanos , Microscopía Electrónica , Tercer Molar , Odontoblastos/ultraestructura , Técnicas de Cultivo de ÓrganosRESUMEN
This fluorescence image analysis method for the quantitative determination of cell adhesion on biomaterials allows bone cells labelled with propidium iodide to be counted automatically, directly on their support. The reliability of the estimation by fluorescence image analysis was validated by comparison with visual counting and with results obtained by an electronic particle counter. In this way it was possible to demonstrate that the adhesive properties of bone cells are dependent on the type of substrate--enstatite (MgO, SiO2, CaO-P2O5-Al2O3), Thermanox (modified polyethyleneterephthalate), or glass. In contrast, the spread of the cell cytoplasm, labelled with fluorescein isothiocyanate and measured by image analysis, does not vary significantly according to the substrate. The characterisation by SKIZ tessellation of the spatial cell arrangement shows that the bone cells have a random organisation on Thermanox and glass, whereas they form aggregates on enstatite.
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Materiales Biocompatibles , Adhesión Celular/fisiología , Cerámica , Vidrio , Tereftalatos Polietilenos , Cráneo/citología , Cráneo/fisiología , Animales , Automatización , Recuento de Células , Feto , Citometría de Imagen , RatasRESUMEN
Tooth organogenesis is dependent on reciprocal and sequential epithelial-mesenchymal interactions and is marked by the appearance of phenotypic matrix macromolecules in both dentin and enamel. The organic matrix of enamel is composed of amelogenins, ameloblastin/amelin, enamelins and tuftelin. Dentin is mainly composed of type I collagen, but its specificity arises from the nature of the non-collagenous proteins (NCPs) involved in mineralization, phosphophoryn (DPP), dentin sialoprotein (DSP), osteocalcin, bone sialoprotein and dentin matrix protein-1 (Dmp1). In this paper, we studied the pattern of expression of four mineralizing protein genes (type I collagen, amelogenin, DSPP and osteocalcin) during the development of rat teeth by in situ hybridization on serial sections. For this purpose, we used an easy and rapid procedure to prepare highly-specific labeled single-stranded DNA probes using asymmetric polymerase chain reaction (PCR). Our results show that type I collagen is primarily expressed in polarizing odontoblasts, followed by the osteocalcin gene expression in the same polarized cells. Concomitantly, polarized ameloblasts start to accumulate amelogenin mRNAs and transiently express the DSPP gene. This latter expression switches over to odontoblasts whereas mineralization occurs. At the same time, osteocalcin gene expression decreases in secretory odontoblasts. Osteocalcin may thus act as an inhibitor of mineralization whereas DSP/DPP would be involved in more advanced steps of mineralization. Amelogenin and type I collagen gene expression increases during dentin mineralization. Their expression is spatially and temporally controlled, in relation with the biological role of their cognate proteins in epithelial-mesenchymal interactions and mineralization.
Asunto(s)
Proteínas de la Matriz Extracelular/genética , Regulación del Desarrollo de la Expresión Génica , Diente/metabolismo , Amelogenina , Animales , Animales Recién Nacidos , Colágeno/genética , Proteínas del Esmalte Dental/genética , Hibridación in Situ , Osteocalcina/genética , Fosfoproteínas/genética , Precursores de Proteínas , ARN Mensajero , Ratas , Ratas Sprague-Dawley , Sialoglicoproteínas/genética , Diente/embriologíaRESUMEN
The aim of this study was to investigate the ultrastructural and immunohistochemical organization of the main collagenous components of healthy human keratinized mucosa surrounding endosseous implants. Eight patients with completely edentulous mandibles were selected. Four endosseous implants were placed in the mandible of each patient, connected with a bar to support a complete overdenture, and loaded 4 months later. Two years after placement, biopsies of surrounding soft tissue, including the sulcular and junctional epithelium with the underlying and supracrestal connective tissue, were routinely prepared for standard electron microscopy and for ultrastructural immunolabeling of Types I, III, and IV collagen. The connective tissue located under the junctional epithelium comprised Types I and III collagen, whereas the supracrestal connective tissue was composed mainly of Type I collagen. Type IV collagen was located exclusively in the basement membrane of the junctional epithelium.