RESUMEN
A methodology employing reversed-phase high-performance liquid chromatography (RP-HPLC) was developed and validated for simultaneous determination of five phenolic compounds in red wine. The chromatographic separation was carried out in a C(18) column with water acidify with acetic acid (pH 2.6) (solvent A) and 20% solvent A and 80% acetonitrile (solvent B) as the mobile phase. The validation parameters included: selectivity, linearity, range, limits of detection and quantitation, precision and accuracy, using an internal standard. All calibration curves were linear (R(2) > 0.999) within the range, and good precision (RSD < 2.6%) and recovery (80-120%) was obtained for all compounds. This method was applied to quantify phenolics in red wine samples from Santa Catarina State, Brazil, and good separation peaks for phenolic compounds in these wines were observed.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Cromatografía de Fase Inversa/métodos , Cinamatos/análisis , Flavonoides/análisis , Vino/análisis , Acetonitrilos , Análisis de Varianza , Brasil , Análisis de los Mínimos Cuadrados , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
Toxigenic fungi were studied in beans (Phaseolus vulgaris L.) of Classes black and color, cultivated in different regions of the State of Santa Catarina, south region of Brazil. The mean counts of filamentous fungi were 2.8 x 103 and 6.7 x 103 CFU/g for beans Classes black and color, respectively. Penicillium spp., Aspergillus spp. and Phoma spp. were the most frequent genera isolated, followed by Ryzopus spp., Alternaria spp., Helminthosporium spp., Cladosporium spp., Botrytis spp., Fusarium spp., Trichoderma spp., Curvularia spp. and Dreschelera spp. Among beans Class black, 24.6 per center of the Aspergillus strains produced mycotoxins: 13.1 per center produced aflatoxins (AFs); 11.5 per center produced ochratoxin A (OTA) and 28.9 per center of Penicillium produced citrinin (CTR). On the other hand, 22.1 per center of Aspergillus strains isolated from beans Class color produced mycotoxins (16.7 per center produced AFs and 5.4 per center produced OTA), while Penicillium genera had 35.4 per center of CTR producing strains. The toxigenic species were A. flavus, A. parasiticus, A. ochraceus and P. citrinum Thom.