RESUMEN
El estudio corresponde a la presentación de 2 casos clínicos con fractura radicular horizontal (tercio apical, tercio 1⁄2), tratadas con el agregado del trióxido mineral (MTA). En ambos casos las piezas sufrieron la fractura por trauma y el MTA se utilizo sin acción quirúrgica, vale decir, se coloco intraconducto. El objetivo del estudio es conocer la viabilidad de la terapia en estos casos, recordando que es necesario realizar nuevas investigaciones para demostrar la efectividad del material.
The study corresponds to the presentation of two clinical cases with horizontally root fractures (apical third, 1⁄2 third) treated with mineral trioxide aggregate (MTA). In both cases the pieces suffered fracture for trauma and the MTA was used without surgical action, is worth saying, its place in root canal. The intention of the study is to know the viability of the therapy in these cases, remembering that it is necessary to realize new investigations to demonstrate the efficiency of the material.
Asunto(s)
Humanos , Compuestos de Aluminio/uso terapéutico , Compuestos de Calcio/uso terapéutico , Fracturas de los Dientes/terapia , Óxidos/uso terapéutico , Silicatos/uso terapéutico , Combinación de Medicamentos , Resultado del TratamientoRESUMEN
Este articulo corresponde a un caso clínico donde se produjo la fractura de un usual instrumento utilizado en una pieza dentaria que se le estaba realizando un tratamiento endodóntico, una fresa Gate Glidden nº1; pero la extracción de este tipo de instrumento hoy en día se hace dificultoso y además se realiza con instrumental de alto costo, pero en este caso clínico utilizamos materiales de bajo valor y mucho ingenio pudiendo realizar el retiro del instrumento que obviamente podría ser usado en cualquier servicio publico donde los recursos son muy limitados y muchas veces austeros.
This article presents to a clinical case where the fracture of a Gate Glidden drill # 1 ocurred during endodontic therapy. Extraction of this type of instrument nowadays is difficult in addition is performed by instruments expensive, but in this clinical case we used materials of low value, and very innovative, being able to extract the instrument. This obviously could be used in any public service where the resources are very limited and often austere.
Asunto(s)
Humanos , Cavidad Pulpar/lesiones , Cuerpos Extraños/terapia , Tratamiento del Conducto Radicular/efectos adversos , Instrumentos Dentales/efectos adversos , Tratamiento del Conducto Radicular/instrumentaciónRESUMEN
Microcin E492 is a low-molecular-weight, channel-forming bacteriocin produced and excreted by Klebsiella pneumoniae RYC492. A 13 kb chromosomal DNA fragment from K. pneumoniae RYC492 was sequenced, and it was demonstrated by random Tn5 mutagenesis that most of this segment, which has at least 10 cistrons, is needed for the production of active microcin and its immunity protein. Genes mceG and mceH correspond to an ABC exporter and its accessory protein, respectively, and they are closely related to the colicin V ABC export system. The microcin E492 system also requires the product of gene mceF as an additional factor for export. Despite the fact that this bacteriocin lacks post-translational modifications, genes mceC, mceI and mceJ are needed for the production of active microcin. Genes mceC and mceI are homologous to a glycosyl transferase and acyltransferase, respectively, whereas mceJ has no known homologue. Mutants in these three genes secrete an inactive form of microcin, able to form ion channels in a phospholipidic bilayer, indicating that the mutation of these microcin genes does not alter the process of membrane insertion. On the other hand, microcin isolated from mutants in genes mceC and mceJ has a lethal effect when incubated with spheroplasts of sensitive cells, indicating that the microcin defects in these mutants are likely to alter receptor recognition at the outer membrane. A model for synthesis and export is proposed as well as a novel maturation pathway that would involve conformational changes to explain the production of active microcin E492.
Asunto(s)
Bacteriocinas/genética , Genes Bacterianos , Klebsiella pneumoniae/genética , Secuencia de Aminoácidos , Antibacterianos/metabolismo , Bacteriocinas/química , Bacteriocinas/metabolismo , Transporte Biológico , Electrofisiología , Canales Iónicos/metabolismo , Klebsiella pneumoniae/metabolismo , Datos de Secuencia Molecular , Familia de Multigenes , Mutagénesis , Sistemas de Lectura Abierta/genética , Péptidos , Alineación de SecuenciaRESUMEN
A gene encoding laccase has been isolated from a genomic library of the white-rot basidiomycete Ceriporiopsis subvermispora constructed in Lambda GEM-11. This gene (Cs-lcs1) contains an open reading frame of 2215 bp, encoding a mature protein of 499 amino acids with a 21-residue signal peptide. The protein sequence exhibits between 63 and 68% identity with laccases from other basidiomycetes and shares with all of them 10 conserved histidines and one cysteine involved in the coordination of copper atoms at the active site of the enzyme. The gene possesses 11 introns, with splicing junctions and internal lariat formation sites adhering to the GT-AG and CTRAY rules, respectively. The upstream region of Cs-lcs1 contains a TATA box, two CAAT sites, five putative metal response elements and a ACE1 element. In agreement with the presence of the latter element, transcription of Cs-lcs1 is activated by copper and silver, as shown by Northern blot and reverse transcription followed by DNA amplification analyses. Based on Southern blot analysis, Cs-lcs1 appears to be the only gene encoding laccase in C. subvermispora.