RESUMEN

Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day. After 1 mo, 91% to 100% resuscitation rates were obtained, while cells that were frozen fast (directly transferred into liquid nitrogen) could not be resuscitated.

Asunto(s)

Técnicas de Cultivo de Célula/métodos , Virus de la Anemia del Pollo/crecimiento & desarrollo , Criopreservación/métodos , Animales , Sangre , Bovinos , Línea Celular , Supervivencia Celular , Pollos , Medios de Cultivo