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The present study aimed to compare by ultrasound imaging (USI) the tibial posterior (TP), medial gastrocnemius (MG) and soleus muscle in patients with and without plantar fasciitis (PF). A sample of 42 individuals was recruited and divided into two groups: PF and a healthy group. The thickness, cross-sectional area (CSA), echointensity and echovariation were assessed in both groups by USI. TP, soleus and MG variables did not report differences (p > 0.05) for thickness and CSA. For the echotexture parameters significant differences were found for MG echointensity (p = 0.002), MG echovariation (p = 0.002) and soleus echointensity (p = 0.012). Non-significant differences (p > 0.05) were reported for soleus echovariation, TP echointensity and TP echovariation variables. The thickness and CSA of the TP, GM and soleus muscle did not show significant differences between individuals with and without PF measured by USI. Muscle quality assessment reported an increase of the MG echointensity and echovariation, as well as a decrease of echointensity of the soleus muscle in the PF group with respect to the healthy group. Therefore, the evaluation of the structure and muscle quality of the extrinsic foot muscles may be beneficial for the diagnosis and monitoring the physical therapy interventions.

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Objective: The primary aim of the present study was to compare the echo intensity (EI) and echovariation (EV) of the intrinsic foot muscles (IFMs) between individuals with and without plantar fasciitis (PF), using ultrasound imaging. The secondary objective was to study the intra-rater reliability of the echotexture variables. Methods: A case−control study was conducted with 64 participants, who were divided into the following two groups: A, the PF group (n = 32); B, the healthy group (n = 32). Results: The comparison between the two groups did not identify significant differences (p > 0.05) between the flexor hallucis brevis (FHB), flexor digitorum brevis (FDB), quadratus plantae (QP) and abductor hallucis brevis (AHB) variables for the EI and EV. Moreover, excellent intra-rater reliability was reported for the following ultrasound imaging EI variables: ABH (ICC = 0.951), FHB (ICC = 0.949), FDB (ICC = 0.981) and QP (ICC = 0.984). Conclusions: The muscle quality assessment using the EI and EV variables did not identify differences in the FHB, FDB, AHB and QP muscles between individuals with and without PF through USI evaluation. The reliability of all the IFM measurements was reported to be excellent.

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ABSTRACT BACKGROUND: Back pain is a normal symptom during pregnancy and is expected to become worse beyond the first three months after childbirth. OBJECTIVES: To determine the effectiveness of wearing unstable shoes instead of conventional shoes, regarding pain intensity, low back mobility and stability, among women with lumbopelvic pain (LPP) during the postpartum period. DESIGN AND SETTING: Prospective, single-blinded, randomized clinical trial conducted at a podiatry and physiotherapy clinical center. METHODS: A nine-week program of wearing either unstable shoes (A) or conventional shoes (B) was implemented. The following outcomes were measured in three assessments: pain intensity, using a visual analogue scale (VAS); low-back mobility, using a modified Schober test; and stability, using a pressure platform. RESULTS: The lateral stability speed, anterior stability speed and anterior center of pressure (COP) showed significant (P < 0.05) decreases in the unstable shoes group after nine weeks, in relation to the conventional group. Intra-group measurements showed significant differences (P < 0.05) in VAS between the second and third assessments and between the first and third assessments in both groups. Intra-group evaluations also showed statistically significant differences (P < 0.05) in the lateral stability speed and anterior stability speed. CONCLUSIONS: Unstable shoes were effective in decreasing the pain intensity at five and nine weeks in women with postpartum LPP. In addition, their use produced decreases in lateral stability speed, anterior stability speed and anterior COP at nine weeks.

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BACKGROUND: Back pain is a normal symptom during pregnancy and is expected to become worse beyond the first three months after childbirth. OBJECTIVES: To determine the effectiveness of wearing unstable shoes instead of conventional shoes, regarding pain intensity, low back mobility and stability, among women with lumbopelvic pain (LPP) during the postpartum period. DESIGN AND SETTING: Prospective, single-blinded, randomized clinical trial conducted at a podiatry and physiotherapy clinical center. METHODS: A nine-week program of wearing either unstable shoes (A) or conventional shoes (B) was implemented. The following outcomes were measured in three assessments: pain intensity, using a visual analogue scale (VAS); low-back mobility, using a modified Schober test; and stability, using a pressure platform. RESULTS: The lateral stability speed, anterior stability speed and anterior center of pressure (COP) showed significant (P < 0.05) decreases in the unstable shoes group after nine weeks, in relation to the conventional group. Intra-group measurements showed significant differences (P < 0.05) in VAS between the second and third assessments and between the first and third assessments in both groups. Intra-group evaluations also showed statistically significant differences (P < 0.05) in the lateral stability speed and anterior stability speed. CONCLUSIONS: Unstable shoes were effective in decreasing the pain intensity at five and nine weeks in women with postpartum LPP. In addition, their use produced decreases in lateral stability speed, anterior stability speed and anterior COP at nine weeks.

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Vasovagal reactions may occur occasionally during electrical stimulation using interferential current (IFC). The purpose of this study was to examine variations in autonomic activity during the application of IFC in asymptomatic participants by analysis of their heart rate variability (HRV). Seventy-three male volunteers were randomly assigned to a placebo group (n = 36; HRV was documented for 10 min, both at rest and during a placebo intervention) and an intervention group (n = 37; HRV was documented for 10 min in two conditions labelled as (1) rest and (2) application of IFC technique on the lumbar segment). The diameters of the Poincaré plot (SD1, SD2), stress score (SS), and the ratio between sympathetic and parasympathetic activity (S/PS) were measured. After interventions, differences amongst the placebo group and the IFC group were found in SD2 (p < 0.001), SS (p = 0.01) and S/PS ratio (p = 0.003). The IFC technique was associated with increased parasympathetic modulation, which could induce a vasovagal reaction. Monitorization of adverse reactions should be implemented during the application of IFC technique. HRV indicators might have a part in prevention of vasovagal reactions. Further studies in patients with lumbar pain are needed to explore possible differences in HRV responses due to the presence of chronic pain.

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Functional connectivity (FC) is typically altered in individuals with Multiple Sclerosis (MS). However, in relapsing-remitting multiple sclerosis (RRMS) patients, the relationship between brain FC, tissue integrity and cognitive impairment is still unclear as contradictory findings have been documented. In this exploratory study we compared both the whole brain connectome and resting state networks (RSNs) FC of twenty-one RRMS and seventeen healthy controls (HCs), using combined network based statistics and independent component analyses. The total white matter (WM) lesion volume and information processing efficiency were also correlated with FC in the RRMS group. Both whole brain connectome and individual RSNs FC were diminished in patients with RRMS compared to HC. Additionally, the reduction in FC was found to be a function of the total WM lesion volume, with greatest impact in those harboring the largest lesion volume. Finally, a positive correlation between FC and information processing efficiency was observed in RRMS. This complimentary whole brain and RSNs FC approach can contribute to clarify literature inconsistencies regarding FC alterations and provide new insights on the white matter structural damage in explaining functional abnormalities in RRMS.

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The aim of this study is to describe the short-term effects of manual lymph drainage (MLD) isolated in supraclavicular area in healthy subjects. A 4-week cross-sectional, double-blinded randomized clinical trial was conducted. Participants: 24 healthy participants between 18 and 30 years old were recruited from Universidad Europea de Madrid from December 2018 to September 2019. A total of four groups were studied: control, placebo, Vodder, and Godoy. The order of the interventions was randomized. Resting Heart Rate and Oxygen Saturation, blood pressure, pressure pain threshold of trapezius muscle, respiratory rate, range of active cervical movements were measured before and after every intervention. All the participants fulfilled four different interventions with a one-week-wash-out period. No statistically significant differences were found between groups in descriptive data; neither in saturation of oxygen, diastolic blood pressure and cervical range of motion. Significant differences were found in favor of Vodder (p = 0.026) in heart rate diminution and in cardiac-rate-reduction. A significant difference in respiratory rate diminution is found in favor of the Godoy group in comparison with the control group (p = 0.020). A significant difference is found in favor of the Godoy group in systolic blood pressure decrease (p = 0.015) even in pressure pain threshold (p < 0.05). MLD decreases systolic blood pressure in healthy participants. However, it does not produce any changes in other physiologic outcomes maintaining physiologic values, which may suggest the safety of the technique in patients suffering from other pathologies.

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Objetivo: refletir sobre a teoria da autoeficácia na amamentação e sua apropriação na prática do enfermeiro. Método: estudo qualitativo, descritivo, cuja coleta dos dados ocorreu a partir de uma revisão de literatura. Após a leitura ampliada e aprofundada sobre a temática, originaram-se duas categorias de reflexão. Resultados: a autoeficácia na amamentação compreende a confiança materna na habilidade para realizar essa prática com sucesso. Estudos demonstram a influência da autoeficácia na decisão, início e manutenção da amamentação. No entanto, esse conhecimento ainda é pouco acessível aos profissionais da saúde que não utilizam essa variável em suas ações junto às mulheres e seus filhos na promoção da amamentação. Conclusão: a reflexão a respeito da autoeficácia na amamentação permite identificar que essa teoria deve ser difundida na prática clínica dos enfermeiros. Evidencia-se a necessidade de que os enfermeiros estejam motivados e abertos para a efetiva utilização da variável autoeficácia materna na prática clínica para a promoção do aleitamento materno.(AU)

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Resumo Objetivo Verificar a associação entre a autoeficácia na amamentação de mães adolescentes e a duração do aleitamento materno exclusivo. Métodos Estudo longitudinal e prospectivo. Para avaliação da autoeficácia na amamentação foi utilizada a versão brasileira da Breastfeeding Self-Efficacy Scale na maternidade, e para avaliação da alimentação da criança foi aplicado um questionário, por meio de contato telefônico, com 30, 60 e 180 dias pós-parto. Resultados 56,90% das participantes apresentaram alto nível de autoeficácia para amamentar. A prevalência AME foi de 62% em 30 dias, 52,59% em 60 dias e 16% em 180 dias pós-parto. Não houve associação significativa entre a confiança com a duração do aleitamento materno exclusivo. Conclusão A confiança não foi um fator preditivo da prevalência do aleitamento materno exclusivo. entre as mães adolescentes. No entanto, evidencia-se a necessidade de novas estratégias para que as adolescentes que passam por intercorrências nestes períodos sejam acolhidas e auxiliadas.

Abstract Objective To verify the association between breastfeeding self-efficacy of adolescent mothers and length of exclusive breastfeeding. Methods Longitudinal and prospective study. The Brazilian version of the Breastfeeding Self-Efficacy Scale was used to evaluate breastfeeding self-efficacy, and a questionnaire was administered through telephone contact at 30, 60 and 180 days postpartum to evaluate child feeding. Results Of the participants, 56.90% presented a high level of breastfeeding self-efficacy. The prevalence of exclusive breastfeeding was of 62% at 30 days, 52.59% at 60 days and 16% at 180 days postpartum. There was no significant association between confidence and length of exclusive breastfeeding. Conclusion Confidence was not a predictive factor in the prevalence of exclusive breastfeeding among adolescent mothers. However, there is an evident need for new strategies so that adolescents who have complications in these periods are embraced and helped.

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Resumo Objetivo Verificar a associação entre a autoeficácia na amamentação e os fatores sociodemográficos e obstétricos das adolescentes. Métodos Estudo observacional, transversal e descritivo, desenvolvido no alojamento conjunto de uma maternidade pública no município de Ribeirão Preto, São Paulo. Os dados foram coletados no período de janeiro a julho de 2014, utilizando a versão brasileira da Breastfeeding Self-Efficacy Scale (BSES) que avaliou a autoeficácia na amamentação. Resultados Os níveis de autoeficácia mais elevados estavam associados às variáveis: ter apoio da mãe ou da sogra no pós-parto (p=0,0083), amamentar na primeira hora de vida (p=0,0244) e estar em aleitamento materno exclusivo no momento da coleta de dados (p=0,0148). Conclusão O apoio da mãe ou da sogra recebido pela puérpera, a amamentação na primeira hora de vida e a prática do aleitamento materno exclusivo durante o período de admissão no alojamento conjunto, influenciaram os níveis de autoeficácia na amamentação entre as puérperas adolescentes.

Abstract Objective To determine the association between breastfeeding self-efficacy and sociodemographic and obstetric factors of adolescents. Methods This observational, cross-sectional and descriptive study was carried out at a public maternity in the municipality of Ribeirão Preto, São Paulo, Brazil. Data were collected from January to July 2014 using the Brazilian version of Breastfeeding Self-Efficacy Scale that evaluated the breastfeeding self-efficacy. Results Higher levels of self-efficacy were associated with the following variables: to be supported by mother or mother-in-law after delivery (p=0.0083), breastfeeding in the first hour of life (p=0.0244) and exclusively breastfeeding upon data collection (p=0.0148). Conclusion The support of mother and mother-in-law received by puerperal women, to breastfeed within the first our and exclusively breastfeed during the admission period in the nursing ward influenced levels of breastfeeding self-efficacy among adolescent puerperal.

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ABSTRACT Objective: to measure and compare the breastfeeding self-efficacy between adolescents and adults mothers in the immediate postpartum. Method: is an observational, cross-sectional and comparative study, developed at a maternity hospital in Ribeirao Preto, Brazil. Data were collected between January and July 2014. The sample consisted of 306 adult mothers and 94 adolescent mothers. The breastfeeding self-efficacy scores were obtained using the Brazilian version of the Breastfeeding Self-Efficacy Scale. Student t-test was used to compare the values of breastfeeding self-efficacy between the groups of participants. We considered a 5% significance level (p=0.05). Results: most adolescents and adults mothers (54%) presented high levels of breastfeeding self-efficacy and there was no statistically significant difference between the scores of the two groups (p=0.3482) . Conclusion: health professionals need to be careful about breastfeeding self-efficacy in order to direct specific actions for each group of mothers (adolescents and adult mothers) to improve the breastfeeding rates.

RESUMEN Objetivo: verificar y comparar la autoeficacia de la Lactancia Materna entre puérperas adolescentes y adultas en el posparto inmediato Método: estudio observacional, transversal y comparativo, realizado en el alojamiento conjunto de una maternidad, en la ciudad de Ribeirão Preto, Brazil. La recolección de datos fue realizada en el período de enero a julio de 2014. La muestra fue constituida por 306 madres adultas y 94 adolescentes. La versión brasileña de la Breastfeeding Self-Efficacy Scale evaluó la autoeficacia de la lactancia materna. Para comparar los valores de autoeficacia entre los grupos participantes, los valores fueron sometidos al test de t-Student. Fue considerado un nivel de significancia de 5% (p=0,05) Resultados: entre los grupos participantes la mayoría (54%) presentó niveles elevados de autoeficacia, y la diferencia entre los resultados entre adolescentes y adultos no fue estadísticamente significativo (p=0,3482) Conclusión: los profesionales deben de estar atentos a la autoeficacia en el amamantamiento, con el objetivo de direccionar acciones específicas a los grupos de mujeres adolescentes y adultas, favoreciendo así, el aumento de los índices de amamantamiento.

RESUMO Objetivo: verificar e comparar a autoeficácia na amamentação entre puérperas adolescentes e adultas no pós-parto imediato. Método: estudo observacional, transversal e comparativo, realizado no alojamento conjunto em uma maternidade, no município de Ribeirão Preto, São Paulo. A coleta de dados foi realizada de janeiro a julho de 2014. Participaram do estudo 306 puérperas adultas e 94 puérperas adolescentes. Para avaliar a autoeficácia na amamentação, foi utilizada a versão brasileira da Breastfeeding Self-Efficacy Scale. O teste t de Student foi usado para comparar os valores de autoeficácia entre os grupos participantes. Foi considerado um nível de significância de 5% (p=0,05). Resultados: entre os dois grupos, a maioria (54%) apresentou autoeficácia elevada, e a diferença entre os escores não foi estatisticamente significativa (p=0,3482). Conclusão: os profissionais devem estar atentos à autoeficácia na amamentação, direcionando ações específicas aos grupos de mulheres adolescentes e adultas, favorecendo assim o aumento dos índices de aleitamento.

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A prática do aleitamento materno e seus benefícios para a saúde materno-infantil tem sido alvo de muitos estudos científicos. Apesar das evidências de benefícios, há a necessidade de se ampliar a visão sobre as questões que envolvem a amamentação, compreendendo este processo como multifatorial. Assim, a idade materna é um dos fatores que influenciam a amamentação, pois mães adolescentes podem apresentar maiores dificuldades para início e manutenção desta prática. Além disso, a confiança materna tem sido identificada como uma variável modificável e protetiva no que tange ao aleitamento materno, já que influencia no seu início, adesão e manutenção. Assim, os objetivos deste estudo foram: verificar a confiança materna para amamentar entre mães adolescentes; identificar a prevalências do aleitamento materno exclusivo nos intervalos de 30, 60 e 180 dias de vida; verificar a associação entre a confiança materna das adolescentes para amamentar e a duração do aleitamento materno exclusivo, nos intervalos de 30, 60 e 180 dias pós-parto. Trata-se de um estudo longitudinal prospectivo, observacional e analítico, realizado no alojamento conjunto do CRSM-MATER, no município de Ribeirão Preto/ SP. A coleta de dados foi realizada entre em janeiro de 2014 a junho de 2015. A amostra foi constituída por 160 mães adolescentes. Para coleta de dados, foram aplicados um questionário sobre informações sociodemográficas e obstétricas e o instrumento Breastfeeding Self-Efficacy Scale (BSES) entre as mães adolescentes admitidas no alojamento conjunto. Posteriormente, estas adolescentes foram acompanhadas por meio de busca fonada em 30, 60 e 180 dias pós-parto, com a utilização do terceiro instrumento de coleta de dados, com questões referentes à alimentação oferecida à criança e intercorrências durante o período de amamentação. Os dados foram analisados com a utilização do programa estatístico Statistical Analysis System SAS® 9.0. Para caracterizar a amostra, a análise dos dados foi fundamentada na estatística descritiva. Para verificar a relação entre a confiança materna e os tempos de amamentação foi realizada a análise de variância (ANOVA) e o Coeficiente de Correlação de Pearson. Para verificar a associação entre as variáveis qualitativas, os dados foram submetidos ao Teste Exato de Fisher. Para todas as análises estatísticas, foram considerados nível de significância de 5%. A maioria das participantes (56,90%) apresentou alto nível de confiança para amamentar. A prevalência do aleitamento materno exclusivo foi de 62% nos 30 dias pós-parto, 52,59% nos 60 dias e 16% aos 180 dias pós-parto. Não houve associação estatisticamente significativa entre a confiança da adolescente com a duração do aleitamento materno exclusivo. Houve associação estatisticamente significativa entre a confiança e as variáveis "intercorrência na gestação" (p=0,0069) e "intercorrência no trabalho de parto e/ou parto"(p=0,0316), ou seja, as adolescentes que não tiveram nenhuma intercorrência na gestação ou no trabalho de parto e/ou parto apresentaram maior confiança na amamentação. Conclui-se que a confiança elevada não foi um fator preditivo da prevalência do AME entre as mães adolescentes, porém foram identificados fatores que influenciaram na confiança para amamentar. Assim, ressalta- se a importância de sua análise na prática clínica com o intuito de fornecer subsídios que favoreçam a melhoria dos índices de amamentação e, consequentemente, da saúde materno- infantil

The practice of breastfeeding and its benefits for maternal and child health has been the subject of many scientific studies. Despite the evidence of benefits, there is the need to expand the vision of the issues surrounding breastfeeding, understanding this process as multifactorial. Thus, maternal age is one of the factors that influence breastfeeding, as teenage mothers may have greater difficulty in initiation and maintenance of this practice. In addition, maternal confidence has been identified as a modifiable and protective variable in relation to breastfeeding, as it influences the beginning, adhesion and maintenance. The objectives of this study were to verify the mother's confidence in breastfeeding among teenage mothers; identify the prevalence of exclusive breastfeeding in the ranges of 30, 60 and 180 days of life; verify the association between maternal breastfeeding confidence to the adolescents and duration of exclusive breastfeeding, in intervals of 30, 60 and 180 days postpartum. This is a prospective longitudinal, observational and analytical, held at the rooming CRSM-MATER, in Ribeirão Preto / SP. Data collection was conducted from January 2014 to June 2015. The sample consisted of 160 nursing mothers adolescents. For data collection, they were applied a questionnaire on sociodemographic and obstetrical information and the Breastfeeding Self- Efficacy Scale tool (BSES) among adolescent mothers admitted to the rooming. Subsequently, these adolescents were accompanied by search in 30, 60 and 180 days postpartum, using the third data collection instrument, with questions relating to food offered to children and complications during the breastfeeding period. Data were analyzed using the statistical program Statistical Analysis System SAS® 9.0. To characterize the sample, the analysis was based on descriptive statistics. To investigate the relationship between maternal confidence and breastfeeding times was performed by analysis of variance (ANOVA) and Pearson's correlation coefficient. To determine the association between the qualitative variables, the data were submitted to Fisher's exact test. For all statistical analyzes were considered 5% significance level. Most participants (56.90%) showed a high level of confidence to breastfeed. The prevalence of exclusive breastfeeding was 62% within 30 days postpartum, 52.59% in 60 days and 16% at 180 days postpartum. There was no statistically significant association between adolescent trust with the duration of exclusive breastfeeding. There was a statistically significant association between the trust and the variables "complications during pregnancy" (p = 0.0069) and "complications during labor and / or childbirth" (p = 0.0316), that is, the teenagers who did not have no complications during pregnancy or labor and / or delivery showed greater confidence in breastfeeding. It is concluded that the high confidence was not a predictor of the prevalence of exclusive breastfeeding among teenage mothers, but were identified factors that influenced the confidence to breastfeed. Thus, we emphasize the importance of his analysis in clinical practice in order to provide subsidies to encourage the improvement of breastfeeding rates and consequently maternal and child health

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BACKGROUND: Oleoma is defined as a foreign body granuloma resulting from the injection of oily substances into the skin or subcutaneous tissue. Liquid injectable silicone has been used extensively over the last five decades for soft tissue augmentation. Although initially considered as a biologically inert material, it has been implicated in a variety of adverse reactions, sometimes with latent periods of decades. MAIN OBSERVATIONS: We describe the case of a 44-year-old Peruvian woman presenting with multiple painless yellowish papules and plaques limited to the lower half of the breasts, that seemed to be cutaneous xanthomas. A skin biopsy showed granulomatous inflammation with multiple clear vacuoles corresponding to injected liquid silicone particles, diagnosed as oleoma. The patient was then re-evaluated and she reported that she underwent breast augmentation by injection of liquid silicone five prior to presentation in our department. Thus, the diagnosis of oleoma was confirmed. CONCLUSIONS: We describe a case with yellowish papules on both breasts in a woman that underwent breast augmentation by injection of liquid silicone. Since this unusual presentation of oleomas can be confused with cutaneous xanthomas, a biopsy is essential for diagnosis.

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Cationic antimicrobial cationic peptides (CAMP) have been found in recent years to play a decisive role in hosts' defense against microbial infection. They have also been investigated as a new therapeutic tool, necessary in particular due to the increasing resistance of microbiological populations to antibiotics. The structural basis of the activity of CAMPs has only partly been elucidated and may comprise quite different mechanism at the site of the bacterial cell membranes or in their cytoplasm. Polymyxin B (PMB) is a CAMP which is effective in particular against Gram-negative bacteria and has been well studied with the aim to understand its interaction with the outer membrane or isolated membrane components such as lipopolysaccharide (LPS) and to define the mechanism by which the peptides kill bacteria or neutralize LPS. Since PMB resistance of bacteria is a long-known phenomenon and is attributed to structural changes in the LPS moiety of the respective bacteria, we have performed a thermodynamic and biophysical analysis to get insights into the mechanisms of various LPS/PMB interactions in comparison to LPS from sensitive strains. In isothermal titration calorimetric (ITC) experiments considerable differences of PMB binding to sensitive and resistant LPS were found. For sensitive LPS the endothermic enthalpy change in the gel phase of the hydrocarbon chains converts into an exothermic reaction in the liquid crystalline phase. In contrast, for resistant LPS the binding enthalpy change remains endothermic in both phases. As infrared data show, these differences can be explained by steric changes in the headgroup region of the respective LPS.

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Smallpox, caused by variola virus, was a devastating disease in humans, but how the virus evolved a strategy to spread to tissue remains unknown. Through the use of microarrays, we identified the gene encoding the Wiskott-Aldrich syndrome protein (WASP), one of the five known WASP family members, which has been induced in the course of infection of human cells with different strains of vaccinia virus (VV) (S. Guerra, L. A. Lopez-Fernandez, A. Pascual-Montano, M. Munoz, K. Harshman, and M. Esteban, J. Virol. 77:6493-6506, 2003; S. Guerra, L. A. Lopez-Fernandez, R. Conde, A. Pascual-Montano, K. Harshman, and M. Esteban, J. Virol. 78:5820-5834, 2004). In a mouse model, we evaluated the role of WASP in infection with VV, a close relative of variola virus. WASP(-/-) (KO) mice infected intranasally and intraperitoneally with VV showed reduced weight loss and mortality compared to wild-type (WT) mice. WASP expression correlated with VV replication in the ovaries but not in the liver or spleen. WT mouse macrophages express WASP but not N-WASP; after VV infection, WASP levels increase threefold. KO macrophages lack N-WASP expression and, when VV infected, are incapable of inducing actin tails and producing extracellular virus. These functions were rescued in KO macrophages after ectopic WASP expression. Overall, our findings demonstrate that WASP has a role in orthopoxvirus infections. Use of WASP proteins for virus spread via the actin tail provides a selective advantage for VV, and probably variola virus, dissemination to distant tissues.

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The potential use of the modified vaccinia virus Ankara (MVA) strain as a live recombinant vector to deliver antigens and elicit protective immune responses against infectious diseases demands a comprehensive understanding of the effect of MVA infection on human host gene expression. We used microarrays containing more than 15,000 human cDNAs to identify gene expression changes in human HeLa cell cultures at 2, 6, and 16 h postinfection. Clustering of the 410 differentially regulated genes identified 11 discrete gene clusters with altered expression patterns after MVA infection. Clusters 1 and 2 (accounting for 16.59% [68 of 410] of the genes) contained 68 transcripts showing a robust induction pattern that was maintained during the course of infection. Changes in cellular gene transcription detected by microarrays after MVA infection were confirmed for selected genes by Northern blot analysis and by real-time reverse transcription-PCR. Upregulated transcripts in clusters 1 and 2 included 20 genes implicated in immune responses, including interleukin 1A (IL-1A), IL-6, IL-7, IL-8, and IL-15 genes. MVA infection also stimulated the expression of NF-kappaB and components of the NF-kappaB signal transduction pathway, including p50 and TRAF-interacting protein. A marked increase in the expression of histone family members was also induced during MVA infection. Expression of the Wiskott-Aldrich syndrome family members WAS, WASF1, and the small GTP-binding protein RAC-1, which are involved in actin cytoskeleton reorganization, was enhanced after MVA infection. This study demonstrates that MVA infection triggered the induction of groups of genes, some of which may be involved in host resistance and immune modulation during virus infection.

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