RESUMEN
BACKGROUND/AIMS: In the absence of other causes, obesity increases the risk of liver disease. We evaluated the prevalence and degree of metabolic and hepatic abnormalities associated with non-alcoholic fatty liver disease (NAFLD) in type II-III obesity in a metropolitan area of South Italy. METHODS: 187 (81 M, 106 F) young adult non-diabetic obese patients, age range 18-50 years (mean 31.9 +/- 8.8), body mass index (BMI) > or =30 (mean 47.5 +/- 9.6), consecutively admitted from January 2000 to April 2003 to the Obesity Outpatients Clinic entered into the study. Patients were divided into two groups: (1) BMI 30.0-39.9, and (2) BMI> or =40. Ultrasound detected liver steatosis was classified as: (I) mild; (II) moderate, and (III) severe. RESULTS: All patients, except 4, showed a variable degree of steatosis: mild was more frequent among females, severe steatosis present only in grade III obesity, with higher prevalence in males than in females (p < 0.001). Mean serum transaminases, in particular alanine aminotransferase (ALT), increased according to BMI and degree of steatosis. Homeostasis Model Assessment (HOMA) index, ferritin and fibrinogen levels increased with BMI, particularly in severe steatosis. In group 2, patients with BMI> or =40 showed a positive correlation between ferritin, aspartate aminotransferase (AST) (r = 0.46, p < 0.018), ALT (r = 0.41, p < 0.036) and gamma-glutamyltransferase (gammaGT) (r = 0.51, p < 0.007), between serum triglycerides (TG) and AST (r = 0.28, p < 0.036), ALT (r = 0.30, p < 0.02) and between HOMA and ALT (r = 0.30, p < 0.03) and gammaGT (r = 0.35, p < 0.012). In group 2 patients with severe steatosis the prevalence of metabolic syndrome according to Adult Treatment Panel III (ATP III) was 40%. CONCLUSION: These data suggest that, in young adult non-diabetic grade III obese patients, fatty liver is always present and strictly related to insulin resistance which, in the presence of severe liver steatosis, is also related to serum ferritin.
Asunto(s)
Hígado Graso/etiología , Ferritinas/sangre , Hígado/enzimología , Obesidad Mórbida/complicaciones , Transaminasas/sangre , Adolescente , Adulto , Alanina Transaminasa/sangre , Índice de Masa Corporal , Hígado Graso/sangre , Hígado Graso/epidemiología , Femenino , Humanos , Resistencia a la Insulina , Italia/epidemiología , Hígado/diagnóstico por imagen , Hígado/patología , Masculino , Persona de Mediana Edad , Obesidad Mórbida/sangre , Obesidad Mórbida/metabolismo , Prevalencia , Índice de Severidad de la Enfermedad , Factores Sexuales , UltrasonografíaRESUMEN
To evaluate whether bioimpedance analysis (BIA) is a useful tool for predicting basal metabolic rate (BMR), sex, age, height, weight, BMI, and single-frequency BIA variables (resistance index and phase angle) were assessed in 61 young adult non-diabetic obese patients (BMI >35 kg/m(2)). BMR was measured by indirect calorimetry. In both sexes BMR significantly correlated with weight, BMI, and resistance index. Using multiple regression analysis, the following prediction equations were derived: (1). considering individual characteristics: BMR (kcal/day)=780+11.4xweight (+221 for men); (2). including also BIA variables: BMR=- 96+8.4xresistance index+8.3xweight+82.5xphase angle. Thus, in young adult, severely obese individuals, BIA variables are significant predictors of BMR.
Asunto(s)
Metabolismo Basal/fisiología , Impedancia Eléctrica , Obesidad Mórbida/fisiopatología , Obesidad/fisiopatología , Adulto , Índice de Masa Corporal , Peso Corporal , Calorimetría Indirecta/métodos , Femenino , Humanos , Masculino , Valor Predictivo de las Pruebas , Análisis de RegresiónRESUMEN
High-performance liquid chromatography purification followed by mass spectrometry analyses highlighted that human senile cataractous lens includes a 8182 Da species which is absent in the normal lens, whereas a 8566/8583 Da species is present in both lenses. Western blot analysis identified both species as ubiquitin. The species at lower molecular weight is a shorter form due to the cleavage of the C-terminal residues 73-76. As it is the last amino acid of ubiquitin which is involved in the protein degradation mechanism, we suggest that this structure modification compromises the function of ubiquitin and consequently the physiologically occurring degradation of the lens proteins.
Asunto(s)
Catarata/etiología , Cristalino/química , Ubiquitina/química , Ubiquitina/fisiología , Anciano , Catarata/metabolismo , Cromatografía Líquida de Alta Presión , Humanos , Modelos Moleculares , Peso Molecular , Mapeo Peptídico , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Ubiquitina/aislamiento & purificaciónRESUMEN
The transglutaminase from rat coagulating gland secretion has been proposed as a new member of the transglutaminase family. Its basal activity is about 11-fold lower than those of other transglutaminases (e.g., the cytosolic tissue transglutaminase), but reaches levels comparable to those of other transglutaminases on addition of specific surfactant agents. There is no study devoted to understanding the molecular basis of this apparently anomalous activation, which is maximal at approximately 1.5 mM sodium dodecyl sulfate. We provide evidence that in the presence of this detergent modifications of the intrinsic fluorescence as well as energy transfer of the protein fluorescence to a micellar probe parallel the activation of the enzyme. As the sodium dodecyl sulfate concentration inducing maximal activation equals the critical micellar concentration, the biological activity of this transglutaminase appears to be modulated by the binding of micellar aggregates. In fact, the enzyme is modified by posttranslational modifications consisting of some lipid tails. At least two of these tails could act as aggregation nuclei of the enzyme with detergents. This behavior is different from that typical of molecular forms purified from other sources.