RESUMEN
Opposing activities of Notch and Wnt signaling regulate mucosal barrier homeostasis and differentiation of intestinal epithelial cells. Specifically, Wnt activity is essential for differentiation of secretory cells including Wnt3-producing Paneth cells, whereas Notch signaling strongly promotes generation of absorptive cells. Loss of caspase-8 in intestinal epithelium (casp8∆int) is associated with fulminant epithelial necroptosis, severe Paneth cell death, secondary intestinal inflammation, and an increase in Notch activity. Here, we found that pharmacological Notch inhibition with dibenzazepine (DBZ) is able to essentially rescue the loss of Paneth cells, deescalate the inflammatory phenotype, and reduce intestinal permeability in casp8∆int mice. The secretory cell metaplasia in DBZ-treated casp8∆int animals is proliferative, indicating for Notch activities partially insensitive to gamma-secretase inhibition in a casp8∆int background. Our data suggest that casp8 acts in the intestinal Notch network.
Asunto(s)
Caspasa 8/metabolismo , Dibenzazepinas/farmacología , Células de Paneth/efectos de los fármacos , Receptor Notch1/antagonistas & inhibidores , Animales , Caspasa 8/genética , Muerte Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Masculino , Metaplasia , Ratones Endogámicos C57BL , Ratones Noqueados , Células de Paneth/enzimología , Células de Paneth/patología , Permeabilidad , Fenotipo , Receptor Notch1/metabolismo , Vías Secretoras , Vía de Señalización Wnt/efectos de los fármacosRESUMEN
Leukocyte recruitment is pivotal for the initiation and perpetuation of inflammatory bowel disease (IBD) and controlled by the specificity and interactions of chemokines and adhesion molecules. Interactions of the adhesion molecules α4ß7-integrin and mucosal addressin cell-adhesion molecule-1 (MAdCAM-1) promote the accumulation of pathogenic T-cell populations in the inflamed intestine. We aimed to elucidate the significance of ß7-integrin expression on innate immune cells for the pathogenesis of IBD. We demonstrate that ß7-integrin deficiency protects recombination-activating gene-2 (RAG-2)-deficient mice from dextran sodium sulfate (DSS)-induced colitis and coincides with decreased numbers of colonic effector monocytes. We also show that ß7-integrin is expressed on most CD11b(+)CD64(low)Ly6C(+) bone marrow progenitors and contributes to colonic recruitment of these proinflammatory monocytes. Importantly, adoptive transfer of CD115(+) wild-type (WT) monocytes partially restored the susceptibility of RAG-2/ß7-integrin double-deficient mice to DSS-induced colitis, thereby demonstrating the functional importance of ß7-integrin-expressing monocytes for the development of DSS colitis. We also reveal that genetic ablation of MAdCAM-1 ameliorates experimental colitis in RAG-2-deficient mice as well. In summary, we demonstrate a previously unknown role of α4ß7-integrin-MAdCAM-1 interactions as drivers of colitis by directing inflammatory monocytes into the colon.