RESUMEN
The increasing global reports on the occurrence of macrolide antibiotics resistance, especially erythromycin (Ery) resistant strains, suggests the possible presence of these antibiotics in the environment hence, their inclusion in the EU watchlist of water pollutants. Consequently, there is an urgent need for the development of portable and cost effective analytical sensing devices for their monitoring in water. The combination of molecularly imprinted polymer (MIP) as a sensing element with a portable electrochemical transducer such as screen printed electrode (SPE) may offer a valuable approach for the desired routine environmental monitoring. This work demonstrates the preparation of an electrochemical MIP-based sensor for Ery detection in aqueous media. Ery-selective MIP, Ery-MIP was generated directly on SPE, Ery-MIP/SPE via electrochemical polymerization of m-phenylenediamine (mPD). The optimization of sensor performance was achieved with special attention given to the selection of functional monomer, template removal, polymer thickness and incubation time. Ery-MIP/SPE sensor demonstrated the ability to discriminate target analyte against very close analogues i.e clarithromycin and azithromycin in both PBS and tap water. In addition, Ery-MIP/SPE could detect Ery down to low limits (LODâ¯=â¯0.1â¯nM and LOQâ¯=â¯0.4â¯nM) and exhibited good recovery in tap water. The presented analytical approach could be potentially suited and/or further developed for adequate monitoring of Ery as well as other macrolides in environmental water.