RESUMEN
Opisthorchiasis is endemic in parts of Southeast Asia, including the northern and northeastern regions of Thailand. In these regions, the transmission by fish intermediate hosts has received little attention. We investigated the intensity of Opisthorchis viverrini metacercariae (OV MC) in wild cyprinid fishes from five districts within the Nakhon Phanom Province, Northeast Thailand. Fishes were procured from local markets in five districts throughout three different seasons (hot, rainy and cold) between February 2018 and January 2019. The samples were identified, counted and weighed before metacercariae detection was performed via the artificial digestion method. A total of 2,149 freshwater fishes, representing 20 species were collected. The fish most commonly contaminated with OV MC were Anematichthys repasson and Hampala dispar. The intensity of OV MC in Nakhon Phanom was 0.23 OV MC/fish and varied among districts, ranging from 0.07 to 0.52. A low intensity of OV MC/fish (defined as <=1 cyst) was found in all three different seasons in the Nakhon Phanom Province; hot season (0.55), cold season (0.22) and the rainy season (0.13). The intensity of OV MC/fish was moderate (defined as >1 cyst) in the Renu Nakhon district (2.5) in the hot season. By fish species, H. dispar yielded the highest, with a moderate intensity of 2.1. In natural freshwater cyprinid fish in Nakhon Phanom, OV MC infection is endemic with intensity rates varying according to district, season and fish species.
Asunto(s)
Cyprinidae , Quistes , Opistorquiasis , Opisthorchis , Animales , Cyprinidae/parasitología , Quistes/parasitología , Quistes/veterinaria , Metacercarias , Opistorquiasis/epidemiología , Opistorquiasis/veterinaria , Prevalencia , TailandiaRESUMEN
@#Opisthorchiasis is endemic in parts of Southeast Asia, including the northern and northeastern regions of Thailand. In these regions, the transmission by fish intermediate hosts has received little attention. We investigated the intensity of Opisthorchis viverrini metacercariae (OV MC) in wild cyprinid fishes from five districts within the Nakhon Phanom Province, Northeast Thailand. Fishes were procured from local markets in five districts throughout three different seasons (hot, rainy and cold) between February 2018 and January 2019. The samples were identified, counted and weighed before metacercariae detection was performed via the artificial digestion method. A total of 2,149 freshwater fishes, representing 20 species were collected. The fish most commonly contaminated with OV MC were Anematichthys repasson and Hampala dispar. The intensity of OV MC in Nakhon Phanom was 0.23 OV MC/fish and varied among districts, ranging from 0.07 to 0.52. A low intensity of OV MC/fish (defined as <1 cyst) was found in all three different seasons in the Nakhon Phanom Province; hot season (0.55), cold season (0.22) and the rainy season (0.13). The intensity of OV MC/fish was moderate (defined as >1 cyst) in the Renu Nakhon district (2.5) in the hot season. By fish species, H. dispar yielded the highest, with a moderate intensity of 2.1. In natural freshwater cyprinid fish in Nakhon Phanom, OV MC infection is endemic with intensity rates varying according to district, season and fish species.
RESUMEN
@# Opisthorchiasis caused by Opisthorchis viverrini infection is a major public health concern in Thailand. Despite many decades of national campaigns in place to reduce and control opisthorchiasis in Thailand, the infections remain to exist particularly in the northern and northeastern parts of the country. This study aimed to evaluate the current prevalence of O. viverrini infection in rural communities in northeast Thailand. A cross-sectional survey was conducted between February and October 2018 in three districts (Na Kae, That Phanom and Wang Yang) in Nakhon Phanom Province, Thailand. Demographic data were collected using a standardised questionnaire. Stool samples were collected and processed using the Kato-Katz technique to determine the presence of O. viverrini and other intestinal parasites. In total, 564 individuals were enrolled. The overall intestinal helminth infections were 15.2% (95% CI: 12.4–18.5). Species distribution included a majority of O. viverrini mono-infections (12.9%), followed by Strongyloides stercoralis (1.4%) and Taenia spp. (0.4%). The prevalence of O. viverrini was significantly higher in Wang Yang district (P = 0.022), in males (P = 0.004) and those previously positive with helminth infections (P<0.001) and received treatment of anti-helminths (P<0.001), than in their counterparts. Multivariate regression analysis revealed that being male (adjusted odds ratio [aOR] 1.77, P = 0.035) and those who previously tested positive for helminth infections (aOR 8.69, P<0.001) were significantly associated with a higher odd of O. viverrini infections, but lower in those who had previous stool examination (aOR 0.22, P = 0.001). This study demonstrated that the updated prevalence of O. viverrini infection is still high in rural communities in northeast Thailand. The data of this study will be useful to guide and improve strategies for future O. viverrini and other helminths prevention and control in this region.
RESUMEN
Opisthorchiasis caused by Opisthorchis viverrini infection is a major public health concern in Thailand. Despite many decades of national campaigns in place to reduce and control opisthorchiasis in Thailand, the infections remain to exist particularly in the northern and northeastern parts of the country. This study aimed to evaluate the current prevalence of O. viverrini infection in rural communities in northeast Thailand. A cross-sectional survey was conducted between February and October 2018 in three districts (Na Kae, That Phanom and Wang Yang) in Nakhon Phanom Province, Thailand. Demographic data were collected using a standardised questionnaire. Stool samples were collected and processed using the Kato-Katz technique to determine the presence of O. viverrini and other intestinal parasites. In total, 564 individuals were enrolled. The overall intestinal helminth infections were 15.2% (95% CI: 12.4-18.5). Species distribution included a majority of O. viverrini mono-infections (12.9%), followed by Strongyloides stercoralis (1.4%) and Taenia spp. (0.4%). The prevalence of O. viverrini was significantly higher in Wang Yang district (P = 0.022), in males (P = 0.004) and those previously positive with helminth infections (P<0.001) and received treatment of anti-helminths (P<0.001), than in their counterparts. Multivariate regression analysis revealed that being male (adjusted odds ratio [aOR] 1.77, P = 0.035) and those who previously tested positive for helminth infections (aOR 8.69, P<0.001) were significantly associated with a higher odd of O. viverrini infections, but lower in those who had previous stool examination (aOR 0.22, P = 0.001). This study demonstrated that the updated prevalence of O. viverrini infection is still high in rural communities in northeast Thailand. The data of this study will be useful to guide and improve strategies for future O. viverrini and other helminths prevention and control in this region.
Asunto(s)
Opistorquiasis/epidemiología , Adolescente , Adulto , Animales , Estudios Transversales , Femenino , Humanos , Masculino , Persona de Mediana Edad , Opisthorchis , Prevalencia , Factores de Riesgo , Población Rural , Tailandia/epidemiología , Adulto JovenRESUMEN
Opisthorchis viverrini infection is still one of the public health problems in Southeast Asia. In Thailand alone, more than three million people are estimated to be currently infected. Opisthorchiasis may cause severe biliary diseases, eventually leading to fatal cholangiocarcinoma. This study aimed to investigate the prevalence and risk factors of opisthorchiasis in two rural communities along the Nam Kam River in the northeastern region of Thailand. A cross-sectional study was conducted in Phon Na Kaeo District of Sakon Nakhon Province and That Phanom District of Nakhon Phanom Province in Thailand between March and June 2017. Faecal samples were collected and examined for the presence of O. viverrini by Kato-Katz technique. Socio-demographic variables and risk factors were collected by a standardised questionnaire. The overall prevalence of O. viverrini among 495 study participants was 15.4% (95% confidence interval [CI] 12.3 - 18.8). The parasite rate was higher in Nakhon Phanom than in Sakon Nakhon (P = 0.009) and differed significantly in adults <45 years old in both provinces (P <0.05). Multivariate regression analyses revealed being labourer (adjusted odd ratio [AOR] 4.7 [95% CI 1.08, 20.38], P = 0.039) and farmer (AOR 5.79 [95% CI 1.45, 31.91], P = 0.015) were positively associated with a higher risk of O. viverrini infection. This study highlights O. viverrini infection as a significant endemic disease with potential health problems among the water-based communities along the Nam Kam River. For country with high O. viverrini transmission, such information will be useful for designing efficient strategic interventions.
RESUMEN
@#Opisthorchis viverrini infection is still one of the public health problems in Southeast Asia. In Thailand alone, more than three million people are estimated to be currently infected. Opisthorchiasis may cause severe biliary diseases, eventually leading to fatal cholangiocarcinoma. This study aimed to investigate the prevalence and risk factors of opisthorchiasis in two rural communities along the Nam Kam River in the northeastern region of Thailand. A cross-sectional study was conducted in Phon Na Kaeo District of Sakon Nakhon Province and That Phanom District of Nakhon Phanom Province in Thailand between March and June 2017. Faecal samples were collected and examined for the presence of O. viverrini by Kato-Katz technique. Socio-demographic variables and risk factors were collected by a standardised questionnaire. The overall prevalence of O. viverrini among 495 study participants was 15.4% (95% confidence interval [CI] 12.3 – 18.8). The parasite rate was higher in Nakhon Phanom than in Sakon Nakhon (P = 0.009) and differed significantly in adults <45 years old in both provinces (P <0.05). Multivariate regression analyses revealed being labourer (adjusted odd ratio [AOR] 4.7 [95% CI 1.08, 20.38], P = 0.039) and farmer (AOR 5.79 [95% CI 1.45, 31.91], P = 0.015) were positively associated with a higher risk of O. viverrini infection. This study highlights O. viverrini infection as a significant endemic disease with potential health problems among the water-based communities along the Nam Kam River. For country with high O. viverrini transmission, such information will be useful for designing efficient strategic interventions.
RESUMEN
Plasmodium falciparum infection causes transient immunosuppression during the parasitaemic stage. However, the immune response during simultaneous infections with both P. vivax and P. falciparum has been investigated rarely. In particular, it is not clear whether the host's immune response to malaria will be different when infected with a single or mixed malaria species. Phenotypes of T cells from mixed P. vivax-P. falciparum (PV-PF) infection were characterized by flow cytometry, and anti-malarial antibodies in the plasma were determined by an enzyme-linked immunosorbent assay. We found the percentage of CD3+delta2+-T cell receptor (TCR) T cells in the acute-mixed PV-PF infection and single P. vivax infection three times higher than in the single P. falciparum infection. This implied that P. vivax might lead to the host immune response to the production of effector T killer cells. During the parasitaemic stage, the mixed PV-PF infection had the highest number of plasma antibodies against both P. vivax and P. falciparum. Interestingly, plasma from the group of single P. vivax or P. falciparum malaria infections had both anti-P. vivax and anti-P. falciparum antibodies. In addition, antigenic cross-reactivity of P. vivax or P. falciparum resulting in antibodies against both malaria species was shown in the supernatant of lymphocyte cultures cross-stimulated with either antigen of P. vivax or P. falciparum. The role of delta2 +/- TCR T cells and the antibodies against both species during acute mixed malaria infection could have an impact on the immunity to malaria infection.