RESUMEN

RhoA and Rac play key and opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus and growth cones of developing neurons and negatively regulates neurite sprouting and axon formation through a Rho signaling pathway. Tctex-1, a dynein light chain implicated in axon outgrowth by modulating actin dynamics and Rac activity, colocalizes and physically interacts with Lfc, thus inhibiting its GEF activity, decreasing Rho-GTP levels, and functionally antagonizing Lfc during neurite formation.

Asunto(s)

Axones/fisiología , Dineínas/metabolismo , Factores de Intercambio de Guanina Nucleótido/metabolismo , Animales , Células CHO , Aumento de la Célula , Células Cultivadas , Cricetinae , Cricetulus , Aparato de Golgi/metabolismo , Conos de Crecimiento/fisiología , Factores de Intercambio de Guanina Nucleótido/genética , Hipocampo/fisiología , Ratones , Neuritas/fisiología , Ratas , Transducción de Señal , Proteína de Unión al GTP rhoA/metabolismo