RESUMEN
Authentication of true (genuine) cow leathers is in high demand to promote merchandise and economic growth. The present study employs RT-PCR-based TaqMan assay to facilitate the identification. Species-specific primers and probes were designed utilizing the existing NCBI data on mitochondrial DNA (mtDNA) genes, particularly the cytochrome b region (Cyt b). Mitochondrial DNA extracted from leather samples of both Bos taurus and Bos indicus and analyzed following the appropriate procedures. The RT-PCR results showed the designed primers and probes are exceptionally precise for cow leather samples. The established detection limit for the assay is estimated as 0.1 ng of DNA. In summary, the amplifiable mtDNA extracted from finished leather enables the identification of authentic cow leathers using the RT-PCR TaqMan assay, representing a pioneering report in this field.
Asunto(s)
Citocromos b , Cartilla de ADN , ADN Mitocondrial , Animales , Citocromos b/genética , Bovinos/genética , ADN Mitocondrial/genética , Cartilla de ADN/genética , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/normasRESUMEN
The present study highlights the comparative catalytic removal of 2,4,6-trichlorophenol (TCP) in the aqueous phase by binary nanoparticles in free as well as entangled forms. In brief, binary nanoparticles comprising Fe-Ni are prepared, characterized, and subsequently entangled in reduced graphene oxide (rGO) for better performances. Optimization studies on the mass of free and rGO-entangled binary nanoparticles with respect to TCP concentration and other environmental factors were carried out. Results suggested that free binary nanoparticles at 40 mg ml-1took 300 min to dechlorinate 600 ppm of TCP, whereas rGO-entangled Fe-Ni particles at the same mass took only 190 min to dechlorinate when the pH was maintained at near neutral. In addition, experiments on the reuse of the catalyst with respect to removal efficiency were carried out, and the results implied that, compared to free form, rGO-entangled nanoparticles exemplify more than 98% of removal efficacy even after 5 times of exposure to 600 ppm TCP concentration. The reduction in percentage removal was observed after the sixth exposure. A sequential dechlorination pattern was assessed and confirmed using high-performance liquid chromatography. Further, the phenol-enriched aqueous phase is exposed toBacillus licheniformisSL10, which degrades the phenol effectively within 24 h. In conclusion, the prepared binary nanoparticles, both in free as well as in rGO-entangled forms, effectively dechlorinate 2,4,6-TCP contaminations in the aqueous phase, but with differences in removal duration. Entanglement also makes it easier to reuse the catalyst. Furthermore, microbial phenol degradation allows the aqueous phase to be free of 2, 4, and 6-TCP contamination and allows for the reuse of treated water.
RESUMEN
2,4-Dichlorophenol (2,4-DCP) is a priority pollutant according to US Environmental Protection Agency. Its use in various chemical industries and its presence in the effluent necessitate effective removal studies. The present study focuses on degradation of 2,4-DCP by phenol adapted bacteria Bacillus licheniformis strain SL10 (MTCC 25059) at a relatively faster rate. The organism exhibited tolerance to 150â ppm of 2,4-DCP and showed a linear relationship between the growth and substrate concentration (µmax 0.022/h) and the inhibitory concentration was 55.74â mg/L. The degradation efficiency of the organism was 74% under optimum conditions but increased to 97% when the growth medium containing nil sodium chloride. The degradation of 2,4-DCP was effected by the action of extracellular cocktail enzyme containing Catechol 2, 3 dioxygenase (C23DO), phenol hydroxylase and Catechol, 1,2 dioxygenase (C12DO). In vitro enzymatic degradation studies exhibit 98% degradation of 50 ppm of 2,4-DCP within 2 h. Analyses of degradation products infer that the chosen organism followed a meta-cleavage pathway while degrading 2,4-DCP. In conclusion, the bacteria Bacillus licheniformis strain SL10 finds potential application in the remediation of 2,4-DCP.
Asunto(s)
Bacillus licheniformis , Clorofenoles , Bacterias , Biodegradación Ambiental , FenolRESUMEN
The present study exemplifies phenol degradation efficacy of the free and encapsulated bacterial isolate, explored the degradation kinetics and storage stability in detail. In brief, isolation, identification and phenol degradation potential of the bacterial made from wastewater treated sludge samples. The organism identified as B. licheniformis demonstrates phenol degradation at a concentration more than 1500 ppm. Optimization of environmental parameters reduces the time taken for degradation considerably. The organism has further been encapsulated using whey protein and the efficacy of encapsulated species suggested that encapsulation protects the cells from high concentration of phenol and at the same time expedite the degradation of the chosen pollutant at appreciable level. The encapsulated species effectively degrade 3000 ppm concentration of phenol within 96 h of incubation. Both pH and temperature stability observed in the encapsulated species suggests the effectiveness of the encapsulation. The encapsulated cells displayed storage stability for a four week period at 4 C and reusability up to three exposures. Degradation effected through intracellular catechol 2,3 dioxygenase. In conclusion, encapsulation of B. licheniformis (i) protects the cells from direct exposure to toxic pollutants; (ii) facilitates the field scale application and (iii) eliminate the practical difficulties in handling wet biomass in field application and assures the best possible way of remediating the phenol contaminated soil.