RESUMEN

An integrated microfluidic device capable of performing a variety of genetic assays has been developed as a step towards building systems for widespread dissemination. The device integrates fluidic and thermal components such as heaters, temperature sensors, and addressable valves to control two nanoliter reactors in series followed by an electrophoretic separation. This combination of components is suitable for a variety of genetic analyses. As an example, we have successfully identified sequence-specific hemagglutinin A subtype for the A/LA/1/87 strain of influenza virus. The device uses a compact design and mass production technologies, making it an attractive platform for a variety of widely disseminated applications.

Asunto(s)

Glicoproteínas Hemaglutininas del Virus de la Influenza/genética , Gripe Humana/genética , Técnicas Analíticas Microfluídicas/instrumentación , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Animales , Cartilla de ADN/química , ADN Viral/metabolismo , Electroforesis , Vidrio , Calor , Humanos , Procesamiento de Imagen Asistido por Computador , Ratones , Microfluídica , Miniaturización , Plásmidos/metabolismo , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Silicio/química , Temperatura , Factores de Tiempo