RESUMEN

Depletion of oxygen (O2) levels and reduction in the ATP synthesis (or even its complete blockage) are important characteristics of mitochondrial dysfunction; features that are often correlated with neurodegeneration. The measurement of oxygen consumption rate (OCR) is thus essential to evaluate cellular metabolism, survival, and neuroprotective strategies. In the present chapter, we describe the oxygen consumption assay using a Clark-type oxygen electrode in different types of samples named cells suspension (from primary and established cell culture), brain slices (ex vivo), and fresh brain tissues. In addition, we demonstrate herein how the program Oxygraph can be used in order to analyze the data and different approaches to normalize it.

Asunto(s)

Adenosina Trifosfato/metabolismo , Bioensayo , Encéfalo/metabolismo , Mitocondrias/metabolismo , Neuronas/metabolismo , Fosforilación Oxidativa , Consumo de Oxígeno , Animales , Encéfalo/efectos de los fármacos , Línea Celular , Humanos , Técnicas In Vitro , Mitocondrias/efectos de los fármacos , Neuronas/efectos de los fármacos , Fosforilación Oxidativa/efectos de los fármacos , Consumo de Oxígeno/efectos de los fármacos , Cultivo Primario de Células , Ratas , Factores de Tiempo