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Mar Environ Res ; 76: 114-21, 2012 May.
Artículo en Inglés | MEDLINE | ID: mdl-22104963

RESUMEN

The potential toxicity of stannum dioxide (SnO2), cerium dioxide (CeO2) and iron oxide (Fe3O4) nanoparticles (NPs) in the marine environment was investigated using the sea urchin, Paracentrotus lividus, as an in vivo model. We found that 5 days after force-feeding of NPs in aqueous solutions, the three NPs presented different toxicity degrees, depending on the considered biomarkers. We examined: 1) the presence of the NPs in the coelomic fluid and the uptake into the immune cells (coelomocytes); 2) the cholinesterase activity and the expression of the stress-related proteins HSC70 and GRP78; 3) the morphological changes affecting cellular compartments, such as the endoplasmic reticulum (ER) and lysosomes. By Environmental Scanning Electron Microscope (ESEM) analysis, coupled with Energy Dispersive X-ray Spectroscopy (EDS) we found that NPs were uptaken inside coelomocytes. The cholinesterases activity, a well known marker of blood intoxication in vertebrates, was greatly reduced in specimens exposed to NPs. We found that levels of stress proteins were down-regulated, matching the observed ER and lysosomes morphological alterations. In conclusion, this is the first study which utilizes the sea urchin as a model organism for biomonitoring the biological impact of NPs and supports the efficacy of the selected biomarkers.


Asunto(s)
Nanopartículas del Metal/toxicidad , Óxidos/toxicidad , Paracentrotus/efectos de los fármacos , Contaminantes Químicos del Agua/toxicidad , Animales , Bioensayo , Biomarcadores/metabolismo , Colinesterasas/metabolismo , Activación Enzimática/efectos de los fármacos , Paracentrotus/citología , Paracentrotus/enzimología , Paracentrotus/metabolismo , Contaminantes Químicos del Agua/metabolismo
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