RESUMEN
Some metabolic peculiarities of bacteria of the genus Klebsiella were investigated. The bacteria under study were isolated from different sources and varied in virulence. The pathogenic and saprotrophic enterobacteria were discriminated based on their response to the addition of carbohydrates or nitrate to the medium. Pathogenic Klebsiella spp. exhibited mainly the mixed formic-acid type of fermentation and were more resistant to nitrates than saprotrophic bacteria with the butanediol type of fermentation. The bacteria Klebsiella pneumoniae subsp. pneumoniae isolated from different sources, such as patients, healthy persons, or the environment, exhibited no substantial differences in metabolism and virulence. It was inferred that these bacteria themselves cannot cause disease, and their isolation in morbid states is an indirect result of dysbacteriosis.
Asunto(s)
Klebsiella/metabolismo , Metabolismo de los Hidratos de Carbono , Fermentación , Klebsiella/patogenicidad , Nitratos/metabolismo , Especificidad de la Especie , VirulenciaRESUMEN
While comparatively analyzing the properties of Klebsiella pneumoniae from three studied sources (patients, healthy people, environment), no reliable differences between the comparable strains have been revealed. No differences have been found in the toxin formation, hemagglutinating and antilysozymic activity, adhesivity and invasive properties as well as in the peculiarities of gas exchange, while growing on media with different nitrogen and carbon sources. The studied microorganisms differed only in antibiotic resistance: strains isolated from the environment were sensitive to antibiotics, whereas strains of human origin were resistant to them.
Asunto(s)
Klebsiella pneumoniae/fisiología , Antibacterianos/farmacología , Adhesión Bacteriana , Células Cultivadas/microbiología , Medios de Cultivo , Microbiología Ambiental , Humanos , Infecciones por Klebsiella/microbiología , Klebsiella pneumoniae/efectos de los fármacos , Klebsiella pneumoniae/aislamiento & purificación , Klebsiella pneumoniae/patogenicidad , Pruebas de Sensibilidad Microbiana , Muramidasa/antagonistas & inhibidores , Factores de TiempoRESUMEN
The pattern of oxido-reductive processes and antifungal properties of the bacteria Bacillus brevis and Pseudomonas mycophaga were studied as related to the type and ratio of nitrogen sources (NH4, NO3) in the nutrient medium. The biosynthesis of antibiotics was coupled with the anaerobic pattern of metabolism, and energetic processes of metabolism varied with respect to the nitrogen source. Nitrates exerted an inhibitory effect on the enzymic systems responsible for utilization of ammonium nitrogen.