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We have investigated the effect of biaxial constraint during glutaraldehyde crosslinking on the equibiaxial mechanical properties of bovine pericardium. Crosslinking of cruciate samples was carried out with: (i) no applied load, (ii) an initial 25 g ( approximately 30 kPa) equibiaxial load, or (iii) an initial 200 g (approximately 250 kPa) equibiaxial load. All loading during crosslinking was done under a defined initial equibiaxial load and subsequently fixed biaxial strain. Load changes during crosslinking were monitored. Mechanical testing and constraint during crosslinking were carried out in a custom-built biaxial servo-hydraulic testing system incorporating four actuators with phase-controlled waveform synthesis, high frame-rate video dimension analysis, and computer-interfaced data acquisition. The paired biaxial stress strain responses under equibiaxial loading at 1 Hz (before and after treatment) were evaluated for changes in anisotropic extensibility by calculation of an anisotropy index. Scanning electron microscopy (SEM) was performed on freeze-fractured samples to relate collagen crimp morphology to constraint during crosslinking. Fresh tissue was markedly anisotropic with the base-to-apex direction of the pericardium being less extensible and stiffer than the circumferential direction. After unconstrained crosslinking, the extensibility in the circumferential direction, the stiffness in the base-to-apex direction, and the tissue's anisotropy were all reduced. Anisotropy was preserved in the tissue treated with an applied 25 g load; however, tissue treated with an applied 200 g load became extremely stiff and nearly isotropic. SEM micrographs correlated well with observed extensibility in that the collagen fibre morphology changed from very crimped (unconstrained crosslinking), to straight (200 g applied load). Biaxial stress-fixation may allow engineering of bioprosthetic materials for specific medical applications.

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A variety of media and methods have been used to store teeth used in bond-strength studies of resin restorative materials to dentine. This study examined the effect of 2 months of storage using 11 different methods and media on the shear-bond strength of Z100 resin composite to bovine dentine mediated by Scotchbond Multi Purpose adhesive. Freshly harvested teeth were used as controls. The results showed that 7 of the l1 storage methods or media were similar in shear-bond strengths and bond-failure characteristics. Four of the 11 methods (irradiation, or storage in thymol, methanol, and glutaraldehyde) resulted in significantly lower shear-bond strengths and atypical shear-bond failure, indicating that these are the least desirable methods of tooth storage for studies of this type. This study has further shown that if insufficient numbers of fresh teeth are available for studies of shear-bond strength, then freezing is the preferred method of storage for the registration of high shear-bond strengths. It is also apparent that further investigations are needed to examine what post mortem changes occur in dentine, whether these changes are modified by various storage conditions, and whether they have any significant effect on bonding of resin composites.

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OBJECTIVES: The purpose of this study was to evaluate the short-(24 h) and long-(180 day) term shear bond strengths of resin to bovine dentine etched with varying dilutions of aqueous solutions of phosphoric and maleic acids. METHODS: Bovine dentine surfaces were prepared using water irrigated #600 grit SiC paper. The dentine surfaces were etched in groups of 10 teeth using various dilutions of phosphoric or maleic acid. Using the Scotchbond Multipurpose System (SBMP; 3M Co., MN, USA) and a wet technique the etched surfaces were primed and unfilled bonding resin (UBR) was applied to the dentine surface within the confines of a gelatin cylinder. A cylinder of filled resin (Z 100; 3M Co., MN, USA) was then photocured and the specimens were then stored in water for 24 h or 180 days prior to shear bond testing to failure. RESULTS: The results showed that high bond strengths were recorded using acids which were more dilute than those commercially available. There were also indications that phosphoric acid is not the etching acid of choice for the SBMP system. CONCLUSIONS: The results of this study showed that high bond strengths can be achieved in dentine using etching acids which are more dilute than those commercially available. Maleic acid appears to be the etchant of choice for the SBMP system.

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OBJECTIVES: Glass ionomer cements are used in clinical dentistry as lining, luting and restorative materials. The precise nature of their bonding mechanism to dentine is unclear. This study is an SEM examination of the effect of the liquid contained in Vitrebond cement (3M Co., MN) on the surface of bovine dentine, with respect to delays in photocuring and washing off with water under pressure or as a gentle stream. The effects of delaying photocuring of the mixed and applied cement for up to 120 s were also examined. RESULTS: The results of this study demonstrated that the liquid component of Vitrebond reacts chemically with dentine in a manner suggestive of an effervescent chemical reaction. This reaction produces plugs in the dentinal tubules which are resistant to dislodgement by water under pressure or by gentle washing. Similarly, a delay in photocuring the mixed and applied cements results in porosity of the cement at the dentine interface. CONCLUSION: The findings in this study suggest that the adhesion of Vitrebond to dentine is primarily chemical in nature and that its mechanical strength is compromised if there are substantial delays in photocuring.

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The subject of this case report is a patient with elevated serum levels of titanium (77 parts/billion [ppb]; normal, 3.3 ppb) and vanadium (0.38 ppb; normal, 0.17 ppb) resulting from excessive wear of a metal-backed patellar component in a total knee arthroplasty. The patellar component was worn through both its polyethylene and metal backing as a result of abnormal contact between the patellar and femoral components. Scanning electron microscopic examination of the ingrowth surface of the patellar component indicated that particle debonding occurred as a result of overloading of the sintered neck regions at the particle-substrate interface, suggesting a possible damage during initial insertion of the device, which may have predisposed it to loosening and abnormal contact with the femoral component. Wear particles resulted in staining of the tissues within the knee and an inflammatory and immune response in the synovium consisting of giant cells and T lymphocytes. The serum metal levels were reduced 22 weeks after replacing the patellar component; however, the titanium level was still slightly elevated (8 ppb).

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The clinical requirements of dentin bonds are that they should be non-permeable to oral fluids, seal dentinal tubules, protect the pulp, and be long lasting and durable. Dentin bonding systems that use acidic agents to remove the smear layer are currently being used. Acid conditioning not only removes the smear layer, but also demineralizes the surface of the intertubular dentin and produces intratubular demineralization and funnelling. A dentin bond is produced when hydrophillic resin monomers infiltrate the dentinal tubules and collagen of the demineralized intertubular zone, producing a hybrid layer. The use of a critical point drying technique and SEM allows a clear visualization of the structure of the hybrid layer. This study showed that currently used hydrophillic resin monomers are unable to completely infiltrate the demineralized zone, and it is speculated that this failure could contribute to microleakage and influence the long-term durability of the bond. It is also apparent that these bonds depend on the mechanical investment of collagen by the infiltrating monomer. Since none of the unfilled resins tested seem capable of completely infiltrating the demineralized collagenous zone, the degree of demineralization produced by the commercial acid concentrations in current use is questioned. More dilute acids than those available commercially are shown to reduce both the degree and depth of demineralization, and we suggest that the resultant thinner layer may lend itself to more complete resin infiltration of the collagen.

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PURPOSE: To examine in vitro the composition of resin tags in etched dentin by SEM and vital staining using Alcian Blue dye. MATERIALS AND METHODS: Bovine and human teeth were used. The dentin surface was exposed using water-irrigated #600 grit SiC paper and etched with varying dilutions of aquaeous solutions of phosphoric and maleic acid. Groups of 10 teeth per acid concentration were embedded in PMMA and Scotchbond Multi-Purpose (SMP) and cylinders of Z100 resin composite were bonded to their surfaces. These teeth were shear tested to failure and the failed surfaces subjected to SEM. Groups of three teeth per acid concentration had SMP and cylinders of Z100 bonded to their surfaces. These teeth were decalcified in concentrated HCL acid. The resultant cylinders and their attached resin tags were either subjected to SEM or stained with Alcian Blue dye. The latter specimens were embedded and prepared for light-microscopic examination. A further three human and three bovine teeth per acid concentration were etched and then prepared for SEM examination. All teeth for SEM were critical point dried. RESULTS: Resin tags were shown to consist of both resin and the glycosaminoglycans (GAG) sheath lining of the dentin tubule. As a result of the SEM observations, three mechanisms are suggested for the formation of resin tags, two of which will probably be found in vivo.

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PURPOSE: To examine how the priming agents of Scotchbond Multi-Purpose (SMP) influenced the wettability of collagen in the demineralized zone and hence the penetration and adaptation of its bonding monomers. MATERIALS AND METHODS: Freshly extracted bovine teeth were ground to dentin with SiC paper. SMP and Z100 resin were used. Shear bond testing was performed as well as scanning electron microscopic evaluation. RESULTS: In contrast to the manufacturer's instructions, a 26.3% increase in shear bond strength was obtained when a second coat of priming agent was applied. This increase was statistically significant at the 5% level of confidence. SEM examination of failed conventionally air dried (CAD) specimens showed the presence of voids in the demineralized layer. SEM examination of failed specimens prepared by a critical point drying (CPD) technique showed that these voids were filled with collagen fibrils. The CPD technique also clearly showed that collagen fibrils were incorporated in the unfilled resin. Although priming agents appear to facilitate the penetration of unfilled resin into the demineralized layer and the dentin tubules, areas of unincorporated collagen fibrils can be seen even after two or more applications of the priming agent.

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Femora from young adult Wistar rats were prepared for both light and electron microscopy. Routinely processed wax sections showed the appearance of cement lines immediately proximal to the resorption surface formed by active osteoclasts and distal to the onset of lamella formation in femoral bone tissue. This early stage of extracellular matrix elaboration at reversal lines was then studied by scanning electron microscopy (SEM) of actively remodeling sites, mainly on trabecular and endosteal surfaces. The resorption surface was shown to comprise a decalcified collagenous mat with individual fibers running either parallel or perpendicular to the surface plane. By examining different, neighboring, areas of resorption lacunae, a temporal sequence of new extracellular matrix production could be established. Before the deposition of new collagen, globular accretions were deposited onto the resorption surface. In areas where individual collagen fibers were oriented perpendicular to the surface plane, this globular matrix was initially deposited on the exposed fiber tips. The globules increased in size and fused laterally to form a continuous cement layer, which not only interdigitated with the collagen mesh of the resorption surface but also provided anchorage for new collagen fibers, which themselves became mineralized. These morphologic results provide a mechanistic explanation of coupling at reversal lines.

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This study critically examined the surface and subsurface effects of selected dentin priming agents. Various dentin priming agents were used and specimens were prepared for SEM examination by conventional air drying or by a freeze fracture and critical point drying technique. The results showed that in specimens prepared by freeze fracture and critical point drying technique the demineralization of the dentin surface and subsurface by priming agents leaves a visible network of collagen fibrils. This technique allows better evaluation of the adaptation of bonding resins to the collagen network of the demineralized layer.

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The effect of different treatments for preparing implant materials was examined by scanning electron microscopy and by contact angle measurements. The materials examined were Ti6A14V alloy, Co-Cr-Mo alloy, A12O3, and synthetic hydroxyapatite. Samples were prepared with solid or porous surfaces of these materials. These were detergent-cleaned and then either autoclaved (steam sterilization), radiation-sterilized, nitric acid-etched, or plasma-cleaned. The results of wettability studies indicated marked changes in surface energy corresponding to the different preparation methods, and differences in surface morphology were also observed. These differences could have significant consequences on in vivo implant behaviour as mediated by tissue-implant interactions.

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The purpose of this study was to determine the effect of hydrogen peroxide on the ability of composite resin to penetrate bovine enamel etched with phosphoric acid. In a previous investigation, the flattened enamel surfaces of extracted bovine incisors were immersed in either saline (control) or 35% hydrogen peroxide (experimental) for 5 or 30 min before or after acid etching with 37% phosphoric acid gel for 60 s. A standard-sized light-cured resin cylinder was then bonded to the enamel surface. The specimens were stored in a water bath at 37 degrees C for 1 day or 7 days, after which the enamel-resin bond was tensile tested to failure. Sixteen of the failed specimens (eight control and eight experimental) were randomly selected for the present scanning electron microscopic study to evaluate the appearance of the resin tags at the resin-enamel interface. In the control specimens, the tags were well defined and contiguous with resin which was uniformly adherent to the enamel surface. In the experimental specimens, large areas of the enamel surface were free of resin. When tags were present, they were fragmented, poorly defined, and penetrated to a lesser depth than in the saline controls. The changes observed suggest that there may be interaction between resin and residual peroxide at or near the enamel surface.

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Plaque inhibition by dilute mouth sprays and dentrifrices containing benzethonium chloride and chlorhexidine gluconate was compared in beagle dogs. Agents with chlorhexidine gluconate produced less plaque than their benzethonium chloride or placebo counterparts, but the differences were not significant when compared to mean control scores registered during interexperimental recovery periods.

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Six systems (one polycarboxylate, one polymethyl methacrylate, one unfilled BIS-GMA resin, two combinations of methyl cyanoacrylate and polymethyl methacrylate, and one combination of unfilled BIS-GMA and filled composite resin) were evaluated for in vitro retention to acid-etched human enamel. Also tested were one unfilled-filled resin combination backed by perforated orthodontic band metal and another unfilled resin backed by stainless steel wire mesh. Significant differences in retention were found. Results show that retention depends pril surface and to resist subsequent chemical degradation.

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