RESUMEN

PREMISE OF THE STUDY: Microsatellite primers were developed for the critically endangered species Dayaoshania cotinifolia (Gesneriaceae) to evaluate population genetic diversity and detect population history. • METHODS AND RESULTS: In our study, 15 primer sets were developed using an enriched genomic library. These are the first microsatellite loci developed for this genus. Genetic diversity was measured using 40 individuals. The number of alleles per locus ranged from one to six, and the incidence of observed heterozygosities was 0.365 and 0.410 in two populations. • CONCLUSIONS: The described markers will be useful in future population genetics studies of this critically endangered species.

Asunto(s)

Marcadores Genéticos , Magnoliopsida/genética , Repeticiones de Microsatélite/genética , Alelos , Secuencia de Bases , Cartilla de ADN , Genes de Plantas , Heterocigoto

RESUMEN

PREMISE OF THE STUDY: Microsatellite primers were developed for the rare Taihangia rupestris (Rosaceae) to evaluate genetic diversity, population genetic structure, mating system, and demographic events of this species. • METHODS AND RESULTS: Ten primer sets were developed using an enriched genomic library and were successfully amplified in T. rupestris var. ciliata and T. rupestris var. rupestris. The number of alleles per locus ranged from 2 to 21; the observed and expected heterozygosities ranged from 0.300 to 0.950 and from 0.328 to 0.956, respectively, in the two varieties. • CONCLUSIONS: The markers described here will be useful for studies of genetic variation, genetic structure, and mating systems of T. rupestris, which are important for the future conservation of this rare species.