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BACKGROUND: Residual ridge resorption presents obstacles in prosthodontic treatment, affecting denture stability and the success of dental implants. Genetic elements, specifically the single nucleotide polymorphism (SNP) 1772C>T variant within the hypoxia-inducible factor 1 subunit alpha (HIF-1α) gene, are hypothesized to contribute to residual ridge resorption progression. Nevertheless, its impact remains insufficiently investigated, especially within the context of South Indian populations. We sought to investigate the connection between SNP 1772C>T and residual ridge resorption (RRR) among fully edentulous individuals, considering demographic factors, genotyping methodologies, and statistical evaluations. METHODS: In a prospective case-control study, we recruited 100 completely edentulous participants from South India. Participants were categorized based on alveolar ridge height. Saliva samples were non-invasively collected for DNA extraction, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was employed to determine genotype distribution using the HphI restriction enzyme. The statistical evaluations comprised the utilization of chi-square and Fisher's exact tests. RESULTS: We observed no significant variations in genotype distributions between the case and control cohorts (CT: p=0.24; CC: p=0.65; TT: p=0.30). The heterozygous genotype CT was prevalent in both groups. CONCLUSIONS: Although we did not observe significant associations between SNP 1772C>T and RRR, our findings imply a genetic predisposition to residual ridge resorption that warrants further exploration. Variations in genetic susceptibility across ethnicities and the influence of other genetic variants on residual ridge resorption require additional investigation. This study lays the groundwork for personalized prosthodontic care by highlighting the potential of genetic analysis in routine dental practice to improve treatment strategies.
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Background: The oral health of individuals is undoubtedly affected by the fact that fungal microorganisms are currently resistant to the conventional antifungal drugs used. Still, there are numerous emerging therapeutic alternatives available like phytotherapy. The phytochemistry of various plant species has indicated that phytochemicals could be a better source of medicine with outstanding antifungal, healing, anti-inflammatory spectrum, and antioxidant properties. Aim: To evaluate the antifungal efficacy of silver nanoparticles of vanilla pods against Candida albicans, Candida glabrata, and Candida krusei and to compare with Fluconazole. Materials and Methods: The vanilla pods were procured from the organic store. The aqueous extracts were prepared. Ten percent of the vanilla extract was utilized to generate the silver nanoparticles. The generated silver nanoparticles were characterized visually based on color change and confirmed by UV spectroscopy. Disk diffusion method was used to determine the antifungal activity of the vanilla pods reinforced silver nanoparticles at different concentrations (20 µl, 40 µ, 40n µ, and 80 µand 80ratioC. albicans, C. glabrata, and C. krusei. The antifungal efficacy of the produced nanoparticles was evaluated against a standard of ketoconazole (30 µ30. Results: Vanillin silver nanoparticles exhibit commendable antifungal activity against C. albicans, C. glabrata which is equivalent to that of ketoconazole, and amplified activity against drug-resistant C. krusei was evident. Conclusion: Silver nanoparticles from vanilla pods were studied and their wider zones of inhibition indicated exemplary antifungal efficacy compared with conventional antifungal drugs like ketoconazole. Clinical significance: Future research on vanillin nanocarrier systems might enhance their stability, bioavailability, and bioactivity. As an outcome, with some promising advances in this area, it would be captivating to pursue a systematic investigation into the potential effects of vanillin at the cellular and molecular levels. This will enable us to further assess its applicability as an active biopharmaceutical ingredient in mouthwashes, probiotic lozenges, and denture resin for use as an effective strategy against the biofilm growth of various Candida species.
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INTRODUCTION: The use of microwave in the field of diagnostic pathology has gained a huge response in recent times. Use of domestic microwave ovens in the same is being widely studied. Unveiling the use of the microwave in improving the staining quality of tissue sections in the field of pathology can aid in precise diagnosis of complex conditions. AIM: The main aim of this study was to study the efficiency of microwave staining to reproduce the tissue architecture compared to that of conventional staining techniques. MATERIALS AND METHODS: Thirty different tissue blocks (including 10 mucocele tissue blocks) were used to prepare 30 pairs of slides for three different stains, namely hematoxylin and eosin, Van Gieson's, 0.1% toluidine blue and periodic acid-Schiff and 10 pairs of slides for mucicarmine stain. From each pair of slides, one slide was stained routinely, and the other was stained inside a microwave. Two pathologists evaluated the stained slides, and the results so obtained were analyzed statistically. RESULTS: Microwave staining considerably cut down the staining time from hours to seconds. Microwave staining showed no loss of cellular and nuclear details, uniform staining characteristics, and was of excellent quality. CONCLUSION: The microwave stained slide showed no significant difference in terms of cell outline clarity, cytoplasmic staining, nuclear outline, nuclear staining, nuclear chromatin, and staining intensity compared to that of routine staining method, and a significant difference was observed in the total staining time consumed by all the stains that were used.
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Lichen planus is a chronic inflammatory mucocutaneous disorder that is seen in skin and oral mucosa. Definitive etiology for oral lichen planus remains unknown. It may or may not be associated with skin lesions. Different clinical patterns such as reticular, plaque, erosive, bullous, and atrophic are seen in oral mucosa of which bullous lichen planus is a rare entity. We present a unique case of bullous lichen planus in a 20-year-old male without skin manifestations along with the review of literature comprising various case reports of bullous lichen planus.