RESUMEN
Recent findings suggest that both host and pathogen manipulate copper content in infected host niches during infections. In this review, we summarize recent developments that implicate copper resistance as an important determinant of bacterial fitness at the host-pathogen interface. An essential mammalian nutrient, copper cycles between copper (I) (Cu(+)) in its reduced form and copper (II) (Cu(2+)) in its oxidized form under physiologic conditions. Cu(+) is significantly more bactericidal than Cu(2+) due to its ability to freely penetrate bacterial membranes and inactivate intracellular iron-sulfur clusters. Copper ions can also catalyze reactive oxygen species (ROS) generation, which may further contribute to their toxicity. Transporters, chaperones, redox proteins, receptors and transcription factors and even siderophores affect copper accumulation and distribution in both pathogenic microbes and their human hosts. This review will briefly cover evidence for copper as a mammalian antibacterial effector, the possible reasons for this toxicity, and pathogenic resistance mechanisms directed against it.
Asunto(s)
Antibacterianos/metabolismo , Bacterias/efectos de los fármacos , Bacterias/inmunología , Cobre/metabolismo , Farmacorresistencia Bacteriana , Interacciones Huésped-Patógeno , Animales , Antibacterianos/toxicidad , Cobre/toxicidad , Humanos , Mamíferos , Oxidación-ReducciónRESUMEN
Many Gram-negative bacteria interact with extracellular metal ions by expressing one or more siderophore types. Among these, the virulence-associated siderophore yersiniabactin (Ybt) is an avid copper chelator, forming stable cupric (Cu(II)-Ybt) complexes that are detectable in infected patients. Here we show that Ybt-expressing E. coli are protected from intracellular killing within copper-replete phagocytic cells. This survival advantage is highly dependent upon the phagocyte respiratory burst, during which superoxide is generated by the NADPH oxidase complex. Chemical fractionation links this phenotype to a previously unappreciated superoxide dismutase (SOD)-like activity of Cu(II)-Ybt. Unlike previously described synthetic copper-salicylate (Cu(II)-SA) SOD mimics, the salicylate-based natural product Cu(II)-Ybt retains catalytic activity at physiologically plausible protein concentrations. These results reveal a new virulence-associated adaptation based upon spontaneous assembly of a non-protein catalyst.
Asunto(s)
Cobre/metabolismo , Escherichia coli/fisiología , Escherichia coli/patogenicidad , Interacciones Huésped-Patógeno , Macrófagos/microbiología , Fenoles/metabolismo , Sideróforos/metabolismo , Tiazoles/metabolismo , Animales , Línea Celular , Quelantes/química , Quelantes/metabolismo , Cobre/química , Escherichia coli/química , Infecciones por Escherichia coli/metabolismo , Infecciones por Escherichia coli/microbiología , Macrófagos/metabolismo , Ratones , Fenoles/química , Sideróforos/química , Superóxido Dismutasa/química , Superóxido Dismutasa/metabolismo , Tiazoles/químicaRESUMEN
Bacterial pathogens secrete chemically diverse iron chelators called siderophores, which may exert additional distinctive functions in vivo. Among these, uropathogenic Escherichia coli often coexpress the virulence-associated siderophore yersiniabactin (Ybt) with catecholate siderophores. Here we used a new MS screening approach to reveal that Ybt is also a physiologically favorable Cu(II) ligand. Direct MS detection of the resulting Cu(II)-Ybt complex in mice and humans with E. coli urinary tract infections demonstrates copper binding to be a physiologically relevant in vivo interaction during infection. Ybt expression corresponded to higher copper resistance among human urinary tract isolates, suggesting a protective role for this interaction. Chemical and genetic characterization showed that Ybt helps bacteria resist copper toxicity by sequestering host-derived Cu(II) and preventing its catechol-mediated reduction to Cu(I). Together, these studies reveal a new virulence-associated function for Ybt that is distinct from iron binding.
Asunto(s)
Cobre/toxicidad , Fenoles/metabolismo , Tiazoles/metabolismo , Escherichia coli Uropatógena/efectos de los fármacos , Escherichia coli Uropatógena/metabolismo , Animales , Dominio Catalítico , Cromatografía Liquida , Infecciones por Escherichia coli/microbiología , Femenino , Regulación Bacteriana de la Expresión Génica/fisiología , Humanos , Ratones , Ratones Endogámicos C3H , Fenoles/química , Unión Proteica , Espectrometría de Masas en Tándem , Tiazoles/químicaRESUMEN
Bacterial pathogens are becoming increasingly resistant to antibiotics. As an alternative therapeutic strategy, phage therapy reagents containing purified viral lysins have been developed against gram-positive organisms but not against gram-negative organisms due to the inability of these types of drugs to cross the bacterial outer membrane. We solved the crystal structures of a Yersinia pestis outer membrane transporter called FyuA and a bacterial toxin called pesticin that targets this transporter. FyuA is a ß-barrel membrane protein belonging to the family of TonB dependent transporters, whereas pesticin is a soluble protein with two domains, one that binds to FyuA and another that is structurally similar to phage T4 lysozyme. The structure of pesticin allowed us to design a phage therapy reagent comprised of the FyuA binding domain of pesticin fused to the N-terminus of T4 lysozyme. This hybrid toxin kills specific Yersinia and pathogenic E. coli strains and, importantly, can evade the pesticin immunity protein (Pim) giving it a distinct advantage over pesticin. Furthermore, because FyuA is required for virulence and is more common in pathogenic bacteria, the hybrid toxin also has the advantage of targeting primarily disease-causing bacteria rather than indiscriminately eliminating natural gut flora.
Asunto(s)
Bacteriófagos/metabolismo , Bacterias Gramnegativas/virología , Mucoproteínas/metabolismo , Proteínas Bacterianas/química , Bacteriocinas/química , Bacteriófagos/fisiología , Membrana Celular/metabolismo , Microscopía por Crioelectrón , Modelos Moleculares , Mucoproteínas/química , Conformación Proteica , Ingeniería de Proteínas , Transporte de Proteínas , Receptores de Superficie Celular/químicaRESUMEN
Metabolomic profiling offers direct insights into the chemical environment and metabolic pathway activities at sites of human disease. During infection, this environment may receive important contributions from both host and pathogen. Here we apply an untargeted metabolomics approach to identify compounds associated with an E. coli urinary tract infection population. Correlative and structural data from minimally processed samples were obtained using an optimized LC-MS platform capable of resolving ~2300 molecular features. Principal component analysis readily distinguished patient groups and multiple supervised chemometric analyses resolved robust metabolomic shifts between groups. These analyses revealed nine compounds whose provisional structures suggest candidate infection-associated endocrine, catabolic, and lipid pathways. Several of these metabolite signatures may derive from microbial processing of host metabolites. Overall, this study highlights the ability of metabolomic approaches to directly identify compounds encountered by, and produced from, bacterial pathogens within human hosts.