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[Laser Raman and infrared spectrum analysis of low-density lipoproteins purified from hen egg yolk].
Guang Pu Xue Yu Guang Pu Fen Xi
; 30(11): 2998-3001, 2010 Nov.
Artículo
en Chino
| MEDLINE
| ID: mdl-21284171
RESUMEN
During the experiment, diversified proteins were separated from hen egg yolk by ammonium sulphate rapid fractionation, and pure LDL was obtained after filtrating through Sephadex G-200 chromatography. After the qualitative detection of SDS-PAGE, the authors discovered that LDL consists of five major apoprotein. The Raman and infrared spectrum showed CH2 asymmetric stretching and symmetric stretching mode. However, the authors found C==O stretching vibrations of protein peptide bonds and N+ (CH3)3 asymmetric stretching vibration from the choline group in phospholipids. Laser Raman and infrared spectrum analysis of LDL provided useful information for studying their structure.