RESUMEN
BACKGROUND: To evaluate the three-dimensional (3D) changes after mandibular setback surgery (MSS) in skeletal Class III malocclusion using cone-beam computed tomography (CBCT) and a structured light-based scanner. METHODS: Twenty-eight adult Korean patients with skeletal Class III malocclusion treated by MSS were evaluated. CBCT and facial scan images were recorded one week before and six months after surgery. To use an identical 3D coordinate system, superimposition was performed, and nine skeletal and 18 soft tissue landmarks were identified. Changes in the landmarks and correlation coefficients and ratios between hard and soft tissue changes were evaluated. Paired t test and Pearson's correlation test were performed. RESULTS: After MSS, the amount of transverse correction was 2.45 mm; mandibular setback, 5.80 mm; and vertical reduction, 1.64 mm at the menton, on average. In the transverse axis, there were significant changes and correlations in the lips and chin and an increasing gradient of ratios from the lower lip to the chin. In the anteroposterior axis, the lower lip and chin moved backward significantly and showed notable correlation with hard tissue movement. In the vertical axis, significant upward movement was observed in the landmarks related to the chin, but only lower facial height was significantly decreased. CONCLUSIONS: Soft tissue changes according to hard tissue movement after MSS exhibited a distinct pattern of an increasing gradient from the lips to the chin in a transverse aspect.
Asunto(s)
Maloclusión de Angle Clase III , Procedimientos Quirúrgicos Ortognáticos , Adulto , Cefalometría , Tomografía Computarizada de Haz Cónico , Cara , Humanos , Imagenología Tridimensional , MandíbulaRESUMEN
Previously, we have identified the C3dg protein as an important player in the pathogenesis of atopic dermatitis (AD). In this study, we aimed to identify critical factors associated with C3dg in human keratinocytes based on high-throughput screening (HTS) approaches. We overexpressed C3dg in HaCaT human keratinocytes and conducted serial HTS studies, including RNA sequencing analysis integrated with antibody-chip arrays and implementation of bioinformatics algorithms (PPI mappings). Cumulatively, these approaches identified several novel C3dg-associated genes and proteins that are thought to be significantly involved in skin diseases including AD. These novel genes and proteins included LPA, PROZ, BLK, CLDN11, and FGF22, which are believed to play important roles in C3dg-associated skin functions in keratinocytes, as well as genes related to the two important pathways of systemic lupus erythematosus and Staphylococcus aureus infection. In particular, FGF22 is a unique gene that was detected and validated in all methods applied in this study. By integrating the datasets obtained from these HTS studies and utilizing the strengths of each method, we obtained new insights into the functional role of C3dg in keratinocytes. The approach used here contributes to clinical understanding of C3dg-associated applications and may also be applicable to treatment of AD.
Asunto(s)
Anticuerpos/metabolismo , Complemento C3b/genética , Complemento C3b/metabolismo , Biología Computacional , Queratinocitos/metabolismo , Fragmentos de Péptidos/genética , Fragmentos de Péptidos/metabolismo , Análisis por Matrices de Proteínas , Análisis de Secuencia de ARN , Algoritmos , Células Hep G2 , HumanosRESUMEN
OBJECTIVE: To evaluate the three-dimensional (3D) perioral soft tissue changes after orthodontic treatment in patients with dentoalveolar protrusion using structured light-based scanners. MATERIALS AND METHODS: Forty-four Korean adults (19 men and 25 women, 21.4 ± 3.4 years) with dentoalveolar protrusion treated by extraction of all four first premolars and then en masse retraction with maximum anchorage were evaluated. Lateral cephalograms and 3D facial scans were obtained before treatment (T1) and immediately after debonding (T2). Superimposition was performed, and 27 perioral landmarks were identified. The 3D changes in the landmarks and ratio of movement of the soft tissue relative to the horizontal incisal tip were evaluated. A paired t-test and one-way analysis of variance were performed. RESULTS: The upper incisors were retracted 5.76 mm and the lower incisors were retracted 4.62 mm (P < .001). The upper lip moved inferoposteriorly, and the lower lip moved superoposteriorly. In the lower lip, upward movement was greater than backward movement (P < .001). The most prominent changes appeared at the greatest bulge area. The relative ratios were 42%-53% in the upper lip area and 22%-82% in the lower lip area. The lip corners moved superoposteriorly (P < .001). Subnasale moved downward (P < .05) and posteriorly (P < .001), while the landmarks under the nostrils moved upward and posteriorly (P < .001). CONCLUSION: Facial scans from white structured light scanners efficiently evaluated 3D perioral soft tissue in dentoalveolar protrusion patients. Backward movement and significant vertical movement of the lip were observed. The nasal and lip angle areas showed considerable changes.