RESUMEN
This study is to investigate the influences of acupuncture for dyspepsia on the Radial Pressure Pulse (RPP) between the Chun, Guan and Chy positions of the right/left wrist. Two series of experiments were designed; for the first series, 30 patients with dyspepsia (Group P) and 30 normal subjects (Group N) were seated to undergo the measurements of pulse pressure waveforms from radial artery by sphygmograph. The parameters of RPP included the spectral energy of 0-10 Hz (SE(0-10 Hz)), 10-50 Hz (SE(10-50 Hz)) and 13-50 Hz (SE(13-50 Hz)). For the second series, acupuncture was administered at the right and left Tsu San Li (St-36) points for the same 30 dyspepsia patients, and then their pulse pressure waveforms were re-examined. The results showed that the SE(0-10 Hz) at Right Guan (RB) (p < 0.05), the SE(10-50 Hz) at RB (p < 0.01), and the SE(13-50 Hz) at RB (p < 0.01) and Left Guan (LB) (p < 0.05) of Group P were significantly greater than that of Group N. After the acupuncture, there were significant decreases in the SE(0-10 Hz) only at RB (p < 0.01), in the SE(10-50 Hz) at RB (p < 0.01), Right Chy (RC) (p < 0.05) and LB (p < 0.05), and in the SE(13-50 Hz) RB (p < 0.01), RC (p < 0.05) and LB (p < 0.01). We concluded that the pulse-frequency spectrum at RB was a more effective characteristic for dyspepsia patients, and the acupuncture had an effect on SE(10-50 Hz) and SE(13-50 Hz) more obviously than that on SE(0-10 Hz).
Asunto(s)
Puntos de Acupuntura , Terapia por Acupuntura , Presión Sanguínea/fisiología , Diagnóstico Diferencial , Dispepsia/diagnóstico , Medicina Tradicional China , Pulso Arterial , Adulto , Dispepsia/terapia , Femenino , Frecuencia Cardíaca , Humanos , Masculino , Persona de Mediana Edad , Arteria Radial/fisiología , MuñecaRESUMEN
Hepatocellular carcinoma is a common type of cancer that is usually associated with poor prognosis. In this study, we examined the in vitro and in vivo mechanisms of the traditional Vietnamese herb Zanthoxylum avicennae on the inhibition of HA22T human hepatocellular carcinoma cell proliferation. HA22T cells were treated with different concentrations of Zanthoxylum avicennae extracts (YBBEs) and analyzed with the MTT assay, western blot analysis, flow cytometry, siRNA transfection assays and co-immunoprecipitation assay. Additionally, the HA22T-implanted xenograft nude mouse model was applied to confirm the cellular effects. YBBEs showed a strong inhibition of HA22T cell viability in a dose-dependent manner and significantly reduced cell proliferation-related proteins as well as induced cell cycle arrest in the G2/M phase. Protein phosphatase 2A (PP2A) siRNA or okadaic acid totally blocked YBBE-mediated cell proliferation inhibition. In addition, an HA22T-implanted nude mouse model further confirmed that YBBEs inhibit HA22T tumor cell growth and downregulate the survival and cell cycle regulating proteins, as well as activate the PP2A protein. Our findings indicate that the inhibition of HA22T cell proliferation by YBBEs is mediated through PP2A activation.
Asunto(s)
Carcinoma Hepatocelular/enzimología , Carcinoma Hepatocelular/patología , Neoplasias Hepáticas/enzimología , Neoplasias Hepáticas/patología , Extractos Vegetales/farmacología , Proteína Fosfatasa 2/metabolismo , Zanthoxylum/química , Animales , Western Blotting , Carcinoma Hepatocelular/tratamiento farmacológico , Ciclo Celular/efectos de los fármacos , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Regulación hacia Abajo/efectos de los fármacos , Activación Enzimática/efectos de los fármacos , Humanos , Neoplasias Hepáticas/tratamiento farmacológico , Masculino , Ratones , Ratones Desnudos , Modelos Biológicos , Ácido Ocadaico/farmacología , Fosforilación/efectos de los fármacos , Fitoterapia , Extractos Vegetales/uso terapéutico , Proteínas Proto-Oncogénicas c-akt/metabolismo , ARN Interferente Pequeño/metabolismo , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
OBJECTIVES: The characteristics of the right/left radial pressure pulse (RPP) at the six diagnosis positions in Chinese medicine are not well documented. The purpose of this study is to investigate the spectral energy and augmentation index (AI) of bilateral RPP at the six diagnosis positions and heart rate variability (HRV) in the normotensives, hypertensives without heart dysfunction (HTN-N), and hypertensives with mild diastolic dysfunction (HTN-A). DESIGN: One hundred and thirty-eight (138) subjects were enrolled in this study. All subjects underwent measurements of brachial arterial blood pressure and RPP of both wrists while seated, and the supine measurement of HRV. AI and spectral energy of RPP as well as HRV were analyzed. RESULTS: The low-frequency component, the spectral HRV parameter, was significantly reduced in HTN-A compared with that in the normotensive group. Radial AI of the six diagnosis positions in HTN-N was significantly higher compared with that in the normotensive group or HTN-A. At the six diagnosis positions, the spectral energy of 0-10 Hz (SE(0-10 Hz)) in both hypertensive groups and 10-50 Hz (SE(10-50 Hz)) in the HTN-A group were significantly higher compared with those in the normotensive group. SE(10-50 Hz) at right Chy, left Chun, and left Guan in the HTN-N group were higher than those in the normotensive group. Within each group, there was a significant difference in the energy proportion, 10-50 Hz% (EP(10-50 Hz%)), between the six positions and a significant reduction only at the left Chun position in both hypertensive groups compared to that in the normotensive group. CONCLUSIONS: It is concluded that the EP(10-50 Hz%) revealed the specific characteristics of RPP and significantly varied at the six positions, and the left Chun position, the position to detect the heart diseases in Chinese medicine, is qualified to discriminate the differences between the normotensive and hypertensive patients.
Asunto(s)
Presión Sanguínea/fisiología , Diagnóstico Diferencial , Frecuencia Cardíaca/fisiología , Hipertensión/diagnóstico , Medicina Tradicional China/métodos , Pulso Arterial , Anciano , Arteria Braquial/fisiopatología , Femenino , Cardiopatías/diagnóstico , Humanos , Hipertensión/fisiopatología , Masculino , Persona de Mediana Edad , Postura , Valores de Referencia , MuñecaRESUMEN
The use of herbs as alternative cancer therapies has attracted a great deal of attention owing to their lower toxicity. Whether Zanthoxylum avicennae (Ying Bu Bo, YBB) induces liver cancer cell apoptosis remains unclear. In this study, we investigated the effect of YBB extracts (YBBEs) on HA22T human hepatocellular carcinoma cells in vitro and in an in vivo mouse xenograft model. HA22T cells were treated with different concentrations of YBBEs and analyzed with Western blot analysis, TUNEL, JC-1 staining and siRNA transfection assays. Additionally, the HA22T-implanted xenograft nude mice model was applied to confirm the cellular effects. YBBEs-induced apoptosis, up-regulated death receptor apoptotic pathway markers as well as mitochondrial proteins, and suppressed the survival proteins in a dose-dependent manner. Pro-survival Bcl-2 family proteins were inhibited and the pro-apoptotic ones were increased. Protein phosphatase 2A (PP2A) siRNA or okadaic acid reversed the YBBEs effects, confirming the role of PP2A in YBBEs-induced HA22T apoptosis. All our experimental evidence indicates that YBBEs significantly promote HA22T apoptosis and reduce tumor sizes in xenograft nude mice via PP2A in a dose-dependent manner.
Asunto(s)
Apoptosis/efectos de los fármacos , Carcinoma Hepatocelular/tratamiento farmacológico , Caspasa 3/metabolismo , Neoplasias Hepáticas/tratamiento farmacológico , Extractos Vegetales/farmacología , Proteína Fosfatasa 2/antagonistas & inhibidores , Proteínas Proto-Oncogénicas c-bcl-2/antagonistas & inhibidores , Zanthoxylum/química , Animales , Western Blotting , Carcinoma Hepatocelular/patología , Caspasa 3/genética , Línea Celular Tumoral , Relación Dosis-Respuesta a Droga , Silenciador del Gen/efectos de los fármacos , Humanos , Etiquetado Corte-Fin in Situ , Neoplasias Hepáticas/patología , Masculino , Ratones , Ratones Desnudos , Corteza de la Planta/química , Extractos Vegetales/química , Proteína Fosfatasa 2/genética , Proteína Fosfatasa 2/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/genética , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , ARN Interferente Pequeño/farmacología , Carga Tumoral/efectos de los fármacos , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
In this article, we analyze the arterial pulse in the spectral domain. A parameter, the spectral harmonic energy ratio (SHER), is developed to assess the features of the overly decreased spectral energy in the fourth to sixth harmonic for palpitation patients. Compared with normal subjects, the statistical results reveal that the mean value of SHER in the patient group (57.7 ± 27.9) is significantly higher than that of the normal group (39.7 ± 20.9) (P-value = .0066 < .01). This means that the total energy in the fourth to sixth harmonic of palpitation patients is significantly less than it is in normal subjects. In other words, the spectral distribution of the arterial pulse gradually decreases for normal subjects while it decreases abruptly in higher-order harmonics (the fourth, fifth and sixth harmonics) for palpitation patients. Hence, SHER is an effective method to distinguish the two groups in the spectral domain. Also, we can thus know that a "gradual decrease" might mean a "balanced" state, whereas an "abrupt decrease" might mean an "unbalanced" state in blood circulation and pulse diagnosis. By SHER, we can determine the ratio of energy distribution in different harmonic bands, and this method gives us a novel viewpoint from which to comprehend and quantify the spectral harmonic distribution of circulation information conveyed by the arterial pulse. These concepts can be further applied to improve the clinical diagnosis not only in Western medicine but also in traditional Chinese medicine (TCM).
RESUMEN
Diagnosis by radial arterial pulse is very important in Traditional Chinese Medicine (TCM). The objective of this study is to evaluate the effects of detection time and position on the parameters of time- and frequency-domain of radial pulse wave and to differentiate between Dyspepsia and Rhinitis by the statistical analysis of two signal types. A sphygmograph was developed to record radial pressure pulse for spectral analysis. The measurements were expressed as the pressure wave and its frequency. In this study, 135 subjects including the controls, Dyspepsias and Rhinitis were enrolled in this study. The signals were taken from three diagnosis positions of both wrists. Seven parameters of pressure wave and two parameters of spectrum analysis were evaluated by ANOVA test and Tukey's test. The results showed that the effects of detection time and position on the parameters taken from pulse wave were inconsistent. No regular rules or relationship could be established. The power spectra of 10-50 Hz and 13-50 Hz from sphygmography revealed a significant effect of health status, position and their interaction. There was a significant difference in the power spectra of 13-50 Hz between the control and Rhinitis, as well as between the control and Dyspepsia at right Guan position. The results of this study strongly suggest that the spectrum of sphygmogram might be more helpful than the pressure wave signals for detection of Dyspepsia and Rhinitis.
Asunto(s)
Presión Sanguínea , Diagnóstico Diferencial , Dispepsia/diagnóstico , Medicina Tradicional China/métodos , Pulso Arterial , Rinitis/diagnóstico , Adulto , Análisis de Varianza , Femenino , Humanos , Masculino , Arteria Radial , Esfigmomanometros , Muñeca , Adulto JovenRESUMEN
This study aims to explore the effects of heat and cold stress on the radial pressure pulse (RPP) and heart rate variability (HRV). The subjects immersed their left hand into 45°C and 7°C water for 2 minutes. Sixty healthy subjects (age 25 ± 4 yr; 29 men and 31 women) were enrolled in this study. All subjects underwent the supine temperature measurements of the bilateral forearms, brachial arterial blood pressure, HRV and RPP with a pulse analyzer in normothermic conditions, and thermal stresses. The power spectral low-frequency (LF) and high-frequency (HF) components of HRV decreased in the heat test and increased in the cold test. The heat stress significantly reduced radial augmentation index (AIr) (P < .05), but the cold stress significantly increased AIr (P < .01). The spectral energy of RPP did not show any statistical difference in 0 ~ 10 Hz region under both conditions, but in the region of 10 ~ 50 Hz, there was a significant increase (P < .01) in the heat test and a significant decrease in the cold test (P < .01). The changes in AIr induced by heat and cold stress were significantly negatively correlated with the spectral energy in the region of 10 ~ 50 Hz (SE(10-50 Hz)) but not in the region of 0 ~ 10 Hz (SE(0-10 Hz)). The results demonstrated that the SE(10-50 Hz), which only possessed a small percentage in total pulse energy, presented more physiological characteristics than the SE(0-10 Hz) under the thermal stresses.
RESUMEN
Retention of water in the stratum corneum of skin epidermis plays an important role in regulation of skin function. Loss of water may decline skin appearance gradually and lead to irregular skin disorders. The root extract of Lithospermum erythrorhizon (LES) is known for its various pharmacological activities. However, the potential skin care effect of LES is not clear. The aim of this study was to evaluate the moisturizing efficacy and skin barrier repairing activity of LES. For this study, 30 healthy Asian females (age 20-30) with healthy skin had applied the test emulsions twice daily over a period of 28 days. The skin properties were measured by skin bioengineering techniques. Our preliminary results indicated that LES show moisturizing effect on skin hydration in a time- and dose-dependent pattern, and the maximum increase in skin humidity was 11.77 +/- 1.18% for emulsion LES5.00. Particularly, LES-containing emulsions significantly improve skin barrier function by decreasing the value of transepidermal water loss (TEWL) in a time- and dose-dependent pattern, and the maximum decrease in TEWL value was 7.68 +/- 0.79% for emulsion LES5.00. Taken together, our data demonstrate that LES is more effective in increasing skin humidity and decreasing the TEWL values, indicating the potential skin care effects of LES.
Asunto(s)
Cosméticos , Lithospermum , Extractos Vegetales , Cuidados de la Piel , Adulto , Deshidratación , Femenino , Humanos , Pomadas , Extractos Vegetales/farmacología , Raíces de Plantas , Recuperación de la Función , Fenómenos Fisiológicos de la Piel , Pérdida Insensible de Agua/efectos de los fármacos , Pérdida Insensible de Agua/fisiologíaRESUMEN
As a highly valued ingredient of Chinese medicinal remedies, musk is used as a detoxification agent and for treating fever, inflammation and swelling, and pain. Muscone (3-methylcyclopentadecanone-1), an odoriferous secretion from the ventral glands of male musk deer, is believed to be the active ingredient. A small amount of muscopyridine is also found in the secretion from the ventral glands of male musk deer. Common counterfeit ingredients are musk xylene, musk ambrette, musk ketone, and diphenhydramine. An extraction/GC-MS protocol/data evaluation scheme was developed and applied to study allegedly musk-containing Musk-Tiger Bone Plaster preparations and musk pods (or grains) from Chinese medicine stores and an airport customs. The content of muscone in a specific sample was estimated based on the percentage of the amount recovered from the first extraction. No muscone or counterfeit ingredients were found in all musk pod (or grain) samples from the customs and in the majority of Musk-Tiger Bone Plaster preparations, while muscone (alone or with counterfeit ingredients) was found in most of the musk pod (or grain) collected from Chinese medicine stores.