RESUMEN

To investigate reliability of calculating percentage of myeloblasts by flow cytometric method, data were obtained from 74 hematologic patients (76 paired data). Myeloblast counts obtained by manual count versus flow cytometry were compared. Our data show that the percentage of myeloblasts in the bone marrow obtained with flow cytometric method correlates well with manual count (correlation coefficient is 0.9912). A very high correlation coefficient means that reliable percentage of myeloblasts in the bone marrow can be obtained by either method alone. Flow cytometry is a useful adjunct (or quality control) to validate manual myeloblast count and vice versa.

Asunto(s)

Células de la Médula Ósea/patología , Examen de la Médula Ósea/métodos , Enfermedades Hematológicas/patología , Células Mieloides/patología , Adolescente , Adulto , Anciano , Colorantes Azulados , Examen de la Médula Ósea/normas , Recuento de Células , Niño , Preescolar , Femenino , Citometría de Flujo , Humanos , Lactante , Masculino , Persona de Mediana Edad , Reproducibilidad de los Resultados