RESUMEN
Fumonisins, being common in occurrence in maize-based feeds, pose a great threat to animal and human health. The present study is aimed at determining the antifungal activity of Lactobacillus plantarum MYS6 against a fumonisin producing fungus, Fusarium proliferatum MYS9. The isolate was subjected to standard tests for determining its probiotic attributes and antifungal properties. L. plantarum MYS6 thrived well at pH 3.0 and 6.0, and exhibited strong resistance up to 3% bile. The isolate showed a high degree of cell surface hydrophobicity corresponding to its strong adhesion to chicken crop epithelial cells. Co-inoculation with the fungus on modified de Man Rogosa Sharpe medium revealed the inhibitory effect of L. plantarum MYS6 on fungal growth and biomass. Observation using scanning electron microscopy showed distortion of hyphal structures, swollen tips and disrupted conidia. Conidia germination inhibition assay restrained germination and showed deformed hyphae. The bioprotective feature of the isolate was evident by the inhibition of fungal development in maize-kernel treated with the cell free supernatant of L. plantarum MYS6. Both the isolate and its extracellular metabolites lowered fumonisin content in feed model up to 0.505 mg/Kg of feed and 0.3125 mg/Kg of feed respectively when compared to the level of 0.870 mg/Kg of feed in control. The major antifungal compounds produced by the isolate were 10-Octadecenoic acid, methyl ester; palmitic acid, methyl ester; heptadecanoic acid, 16-methyl ester; stearic acid and lauric acid. L. plantarum MYS6 reduced 61.7% of fumonisin possibly by a binding mechanism. These findings suggest the application of L. plantarum MYS6 as an efficient probiotic additive and biocontrol agent in feed used in poultry industry. Additionally, the antifungal metabolites pose a conspicuous inhibition of Fusarium growth and fumonisin production.
Asunto(s)
Alimentación Animal/microbiología , Antifúngicos , Fumonisinas/metabolismo , Fusarium , Lactobacillus plantarum/fisiología , Aves de Corral , Animales , Antifúngicos/metabolismo , Células Cultivadas , Pollos , Fumonisinas/antagonistas & inhibidores , Fusarium/crecimiento & desarrollo , Fusarium/metabolismo , Inactivación Metabólica , Lactobacillus plantarum/metabolismo , Pruebas de Sensibilidad Microbiana , Aves de Corral/microbiología , Probióticos , Zea mays/microbiologíaRESUMEN
Organismal lifespan is a complex trait that is governed by both its genetic makeup as well as the environmental conditions. The improved socioeconomic condition of humans has led to many lifestyle changes that in turn have altered the demography that includes postponement of procreation. Late age progeny is shown to suffer from many congenital diseases. Hence, there is a need to identify and evaluate natural molecules that could enhance reproductive health span. We have used the well-established model organism, Drosophila melanogaster, and ascertained the consequence of diet supplementation with curcumin. Flies reared on curcumin-supplemented diet had significantly higher lifespan. The progeny of flies reared on curcumin had a higher viability. The activity of a key mitochondrial enzyme-aconitase was significantly higher in flies reared on curcumin-supplemented diet. The results suggest that curcumin can not only correct a key step in the citric acid cycle and help in the release of additional energy but also permanently correct developmental and morphogenetic processes.
Asunto(s)
Envejecimiento/genética , Curcumina/farmacología , Drosophila melanogaster/genética , Longevidad/genética , Reproducción/efectos de los fármacos , Envejecimiento/efectos de los fármacos , Animales , Suplementos Dietéticos , Inhibidores Enzimáticos/farmacología , Femenino , Longevidad/efectos de los fármacos , Masculino , FenotipoRESUMEN
Darwinian fitness in holometabolous insects like the fruit fly Drosophila melanogaster is reported to be positively correlated with body size. If large individuals in a population have higher fitness, then one would expect directional selection to operate leading to uniformly large individuals. However, size polymorphism persists in nature and needs further probing. We assessed the effect of body size on some of the fitness and fitness-related traits in replicate populations of genotypically large, genotypically small and phenotypically small D. melanogaster flies. In this study, the time taken to attain reproductive maturity and copulation duration were independent of fly size. Fecundity and longevity of large females were significantly higher when they partnered genotypically small males than when they were with genotypically larger or phenotypically small males. The increased female longevity when in association with genotypically small males was not due to selective early death of males that would release the female partner from presumed cost of persistent courtship. On the contrary, the genotypically as well as phenotypically small males had significantly higher longevity than large males. The virility of the genotypically small males was not significantly different from that of genotypically large males. Our results clearly show that selection on body size operates in the opposite direction (disruptive selection) for the two genders, thus explaining the persistence of size polymorphisms in the holometabolous insect, Drosophila melanogaster.
Asunto(s)
Tamaño Corporal/fisiología , Drosophila melanogaster/fisiología , Aptitud Genética/fisiología , Selección Genética , Caracteres Sexuales , Animales , Pesos y Medidas Corporales , Copulación/fisiología , Femenino , Fertilidad/fisiología , Aptitud Genética/genética , Longevidad/fisiología , Masculino , Ovario/citología , Maduración Sexual/fisiologíaRESUMEN
Antivenom therapy is the current best therapy available for the treatment of fatal snake envenomation. However, the antivenom offers less or no protection against local effects such as extensive edema, hemorrhage, dermo-, myonecrosis and inflammation at the envenomed region. Viperidae snakes are highly known for their violent local effects and such effects have been commonly treated with plant extracts without any scientific validation in rural India. In this investigation Morus alba plant leaf extract has been studied against the Indian Vipera/Daboia russelii venom induced local and systemic effects. The extract completely abolished the in vitro proteolytic and hyaluronolytic activities of the venom. Edema, hemorrhage and myonecrotic activities were also neutralized efficiently. In addition, the extract partially inhibited the pro-coagulant activity and completely abolished the degradation of Aalpha chain of human fibrinogen. Thus, the extract processes potent antisnake venom property, especially against the local and systemic effects of Daboia russelii venom.