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1.
Mater Sci Eng C Mater Biol Appl ; 58: 666-74, 2016 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-26478358

RESUMEN

Immunosensors based on gold nanoparticles and reduced graphene oxide (AuNPs/rGO)-modified screen-printed electrodes (SPEs) were successfully synthesized using an electrochemical deposition method. The modified SPEs were characterized using a field emission scanning electron microscope (FESEM) and Raman spectroscopy to analyze the morphology and composition of AuNPs and rGO. Both the FESEM and Raman spectroscopy revealed that the AuNPs were successfully anchored on the thin film of rGO deposited on the surface of the SPEs. Characterization with a ferri-ferrocyanide couple [Fe(CN)6(3-/4-)] showed that the electron transfer kinetic between the analyte and electrode was enhanced after the modification with the AuNPs/rGO composite on the electrode surface, in addition to increasing the effective surface area of the electrode. The modified SPE was immobilized with a sandwich type immunosensor to mimic the ELISA (enzyme-linked immunosorbent assay) immunoassay. The modified SPE that was fortified with the sandwich type immunosensor exhibited double electrochemical responses in the detection of carcinoembryonic antigen (CEA), with linear ranges of 0.5-50 ng/mL and 250-2000 ng/mL and limits of detection of 0.28 ng/mL and 181.5 ng/mL, respectively.


Asunto(s)
Técnicas Biosensibles/métodos , Antígeno Carcinoembrionario/análisis , Técnicas Electroquímicas/métodos , Oro/química , Grafito/química , Nanopartículas del Metal/química , Animales , Anticuerpos Inmovilizados , Técnicas Biosensibles/instrumentación , Técnicas Electroquímicas/instrumentación , Electrodos , Límite de Detección , Modelos Lineales , Ratones , Albúmina Sérica Bovina
2.
Asia Pac J Clin Oncol ; 11(2): 160-9, 2015 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-25865669

RESUMEN

AIM: The prognostic significance of KRAS, NRAS, PIK3CA and BRAF mutations was evaluated in Chinese patients with metastatic colorectal cancer (CRC). METHOD: Tumor samples from 183 patients were retrospectively tested for KRAS, NRAS, PIK3CA and BRAF mutations. Multivariate analysis was performed to determine the relationship between mutational status, drug response and survival. RESULT: Over 70% of patients received two or more lines of chemotherapy, 50% had cetuximab and 18% had bevacizumab. The prevalence of KRAS, NRAS, BRAF and PIK3CA mutations was 45, 3.2, 5 and 20%, respectively. For the entire cohort, the median overall survival was 24 months (95% confidence interval [CI] = 20.4-26.4 months). Of the genes tested, only KRAS mutation was an independent prognostic factor with a multivariate hazard ratio of 1.5 (95% CI = 1.05-2.16, P = 0.03). In the subgroup of patients who received cetuximab-based therapy in the first-line setting, KRAS mutation was associated with a lack of response to chemotherapy (28% vs 66%, chi-square, P = 0.01). Patients with KRAS mutant tumors (or KRAS wild-type tumors that harbored BRAF and/or PIK3CA mutations) tended to have lower response rates to chemotherapy and/or cetuximab (P = not significant). The number of NRAS mutant cases was too small to allow any statistical analysis. CONCLUSION: The prevalence of KRAS, NRAS, BRAF and PIK3CA mutations in this cohort is consistent with reports from non-Asian populations, and KRAS mutation has both prognostic and predictive significance in Chinese patients with metastatic CRC.


Asunto(s)
Neoplasias Colorrectales/genética , Neoplasias Colorrectales/metabolismo , Fosfatidilinositol 3-Quinasas/genética , Proteínas Proto-Oncogénicas B-raf/genética , Proteínas Proto-Oncogénicas/genética , Proteínas ras/genética , Adulto , Anciano , Anciano de 80 o más Años , Pueblo Asiatico , Fosfatidilinositol 3-Quinasa Clase I , Neoplasias Colorrectales/patología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Mutación , Metástasis de la Neoplasia , Pronóstico , Estudios Retrospectivos , Adulto Joven
3.
Int J Tuberc Lung Dis ; 16(4): 546-52, 2012 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-22325953

RESUMEN

SETTING: High lung cancer mortality is observed among female never-smokers in Hong Kong. OBJECTIVE: To examine the relationship between obstructive lung disease (chronic obstructive pulmonary disease and/or asthma) and lung cancer mortality by sex and smoking status. DESIGN: A cohort of elderly clients (aged ≥65 years) in a health maintenance programme were followed prospectively through linkage with the territory-wide death registry for causes of death, using identity card number as the unique identifier. RESULTS: After 516,055 person-years of follow-up, respectively 1297, 872 and 1908 deaths were caused by lung cancer, other tobacco-related malignancies and non-tobacco-related malignancies. In the overall analysis, obstructive lung disease was independently associated with mortality due to lung cancer (aHR 1.86, P < 0.001) after adjustment for potential confounders. However, no association was detected among female never-smokers (HR 0.97, P = 0.909), in sharp contrast with female ever-smokers, male never-smokers and male ever-smokers (HR 1.98, 2.34 and 2.09, respectively, P from 0.047 to <0.001). Consistent results were observed after exclusion of all deaths in the initial 3 years. CONCLUSION: Obstructive lung disease exerted differential effects on lung cancer mortality across different sex and smoking subgroups in this Asian population, with a conspicuous absence of effect among female never-smokers.


Asunto(s)
Asma/complicaciones , Neoplasias Pulmonares/mortalidad , Enfermedad Pulmonar Obstructiva Crónica/complicaciones , Fumar/efectos adversos , Anciano , Pueblo Asiatico/estadística & datos numéricos , Femenino , Estudios de Seguimiento , Sistemas Prepagos de Salud , Hong Kong/epidemiología , Humanos , Neoplasias Pulmonares/etiología , Masculino , Estudios Prospectivos , Factores de Riesgo , Factores Sexuales , Fumar/epidemiología , Sobrevida
4.
Hong Kong Med J ; 17(4): 280-5, 2011 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-21813895

RESUMEN

OBJECTIVE: To review the site of involvement, clinical presentation, and treatment outcome of patients having immunoglobulin G4-related sclerosing disease in a local regional hospital. DESIGN: Retrospective case series. SETTING: Pamela Youde Nethersole Eastern Hospital, Hong Kong. PATIENTS: All patients with a diagnosis of immunoglobulin G4-related sclerosing disease in the hospital diagnosed in the period from April 2008 to March 2010. RESULTS: A total of 12 patients with involvement of various organs were identified. There was a male predominance (male-to-female ratio=5:1). The mean age at diagnosis was 65 years. The salivary glands, biliary tract, pancreas, and cervical lymph nodes were the commonest involved sites. The immunoglobulin G4 level was elevated in 83% of the patients. Patients usually appeared to respond well to steroid treatment. CONCLUSION: Immunoglobulin G4-related sclerosing disease is a systemic disease and can involve various systems.


Asunto(s)
Inmunoglobulina G/sangre , Esclerosis/inmunología , Corticoesteroides/uso terapéutico , Anciano , Femenino , Humanos , Masculino , Estudios Retrospectivos , Esclerosis/tratamiento farmacológico , Resultado del Tratamiento
5.
Epilepsy Behav ; 18(3): 179-85, 2010 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-20537594

RESUMEN

The production of new neurons continues throughout adulthood in the dentate gyrus of the hippocampal formation, and is believed to play a role in hippocampus-dependent learning and memory. Seizure-induced changes in adult neurogenesis have been examined primarily in convulsive rodent seizure models, but not in models of nonconvulsive absence seizures. This study examined progenitor cell proliferation in the gamma-hydroxybutyrate (GHB) model of typical absence seizures and the AY-9944 model of atypical absence seizures, and compared these results with changes seen in the rat amygdala kindling model. Kindled subjects were found to have 189% more proliferating cells than sham-kindled control subjects, whereas no significant difference was found between the GHB or AY-9944 model and control subjects. These results suggest that changes in adult neurogenesis in models of absence seizures do not occur, and that seizure-induced enhancement of neurogenesis could depend on the characteristics of the seizure discharge.


Asunto(s)
Giro Dentado/fisiopatología , Epilepsia Tipo Ausencia/patología , Excitación Neurológica/fisiología , Neurogénesis/fisiología , 4-Butirolactona/farmacología , 4-Butirolactona/uso terapéutico , Animales , Animales Recién Nacidos , Anticolesterolemiantes/uso terapéutico , Bromodesoxiuridina/metabolismo , Recuento de Células/métodos , Modelos Animales de Enfermedad , Electroencefalografía/métodos , Epilepsia Tipo Ausencia/tratamiento farmacológico , Epilepsia Tipo Ausencia/etiología , Regulación de la Expresión Génica , Masculino , Proteínas del Tejido Nervioso/metabolismo , Neurogénesis/efectos de los fármacos , Ratas , Ratas Long-Evans , Diclorhidrato de trans-1,4-Bis(2-clorobenzaminometil)ciclohexano/uso terapéutico
6.
Surg Endosc ; 22(3): 777-83, 2008 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-17704882

RESUMEN

BACKGROUND: Endoscopic submucosal dissection (ESD) has emerged as a novel technique for achieving en bloc resection for early esophageal or gastric carcinoma limited to the mucosa. The authors report their experience with a combination of various devices to treat early neoplasia of the foregut using the ESD technique. METHODS: In this prospective case series, ESD was performed for early esophageal or gastric carcinoma limited to the mucosa. These lesions were staged by endoscopic ultrasonography before resection. Magnifying endoscopy and chromoendoscopy were used to locate the tumor and define the margin. The resection was accomplished with submucosal dissection using the insulated tip knife, the hook knife, and the triangular tip knife. The resected specimen was examined systematically for the lateral and deep margins. RESULTS: From January 2004 to March 2006, ESD was performed to manage 30 cases of early gastric or esophageal carcinoma. For 29 of these patients, R0 resection was successfully achieved. The mean operating time was 84.6 min. One patient experienced reactionary hemorrhage 12 h after resection, which was controlled endoscopically. There was no perforation. Most of the circumferential mucosal incisions were performed using the insulated tip knife (76.6%), whereas submucosal dissection was accomplished with a combination of various knives. One of the specimens showed involvement of the lateral margin, whereas another patient had two areas of new early gastric cancer 6 months after the initial procedure. These patients received salvage laparoscopically assisted gastrectomy. CONCLUSIONS: Endoscopic submucosal dissection to manage early neoplasia of the foregut can be achieved safely and effectively with a combination of knives.


Asunto(s)
Endoscopios , Neoplasias Esofágicas/cirugía , Esofagoscopía/métodos , Gastroscopía/métodos , Neoplasias Gástricas/cirugía , Adulto , Anciano , Anciano de 80 o más Años , Procedimientos Quirúrgicos del Sistema Digestivo/instrumentación , Procedimientos Quirúrgicos del Sistema Digestivo/métodos , Disección/instrumentación , Disección/métodos , Neoplasias Esofágicas/patología , Esofagoscopía/efectos adversos , Femenino , Estudios de Seguimiento , Mucosa Gástrica/patología , Mucosa Gástrica/cirugía , Gastroscopía/efectos adversos , Humanos , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Estadificación de Neoplasias , Estudios Prospectivos , Medición de Riesgo , Sensibilidad y Especificidad , Neoplasias Gástricas/patología , Resultado del Tratamiento
7.
Radiat Prot Dosimetry ; 122(1-4): 160-2, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-17132662

RESUMEN

The present paper studied the feasibility of applying comet assay to evaluate the DNA damage in individual HeLa cervix cancer cells after alpha-particle irradiation. We prepared thin CR-39 detectors (<20 microm) as cell-culture substrates, with UV irradiation to shorten the track formation time. After irradiation of the HeLa cells by alpha particles, the tracks on the underside of the CR-39 detector were developed by chemical etching in (while floating on) a 14 N KOH solution at 37 degrees C. Comet assay was then applied. Diffusion of DNA out of the cells could be generally observed from the images of stained DNA. The alpha-particle tracks corresponding to the comets developed on the underside of the CR-39 detectors could also be observed by just changing the focal plane of the confocal microscope.


Asunto(s)
Ensayo Cometa/instrumentación , Daño del ADN , ADN/genética , ADN/efectos de la radiación , Radiometría/instrumentación , Transductores , Partículas alfa , Células Cultivadas , Ensayo Cometa/métodos , ADN/química , ADN/ultraestructura , Diseño de Equipo , Análisis de Falla de Equipo , Células HeLa , Humanos , Radiobiología/instrumentación , Radiobiología/métodos , Radiometría/métodos , Reproducibilidad de los Resultados , Sensibilidad y Especificidad
10.
Aliment Pharmacol Ther ; 20(1): 45-9, 2004 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-15225170

RESUMEN

BACKGROUND: Non-alcoholic fatty liver disease is prevalent in affluent countries and is a cause of cirrhosis and possibly hepatocellular carcinoma. AIM: To examine the clinical and histological features of biopsy-proven non-alcoholic fatty liver disease and investigate the predictors of severe histological disease in Chinese patients. METHODS: Electronic records of all patients (n = 247) who underwent liver biopsy between 1996 and 2003 in our hospital were retrieved. Patients who had histological features of non-alcoholic fatty liver disease were identified. The demographic, clinical, laboratory and histological (Brunt's criteria) parameters of these patients were analysed. RESULTS: Forty-two patients had histology-proven non-alcoholic fatty liver disease. The median age was 47 years (range 23-69). All except one patient had features of metabolic syndrome. The median alanine aminotransferase was 93 (range 24-270) IU/L. Thirty-six (85.7%) patients had steatohepatitis and 11 (26.1%) also had fibrosis. Only one patient had stage 3 fibrosis. The presence of diabetes mellitus predicted higher grade steatohepatitis and fibrosis (P = 0.019) whereas alanine aminotransferase level had no correlation with histological severity of steatohepatitis. After a median follow-up of 42 months, no patient developed hepatic decompensation. CONCLUSIONS: Most Chinese patients with non-alcoholic fatty liver disease had features of the metabolic syndrome. Histological activity was generally mild. Diabetes mellitus was the most important predictor of severe histological disease.


Asunto(s)
Hígado Graso/etnología , Adulto , Anciano , China/etnología , Hígado Graso/patología , Femenino , Hepatitis/etnología , Hepatitis/patología , Hong Kong/etnología , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos
11.
J Clin Pathol ; 57(3): 260-5, 2004 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-14990596

RESUMEN

BACKGROUND: Severe acute respiratory syndrome (SARS) became a worldwide outbreak with a mortality of 9.2%. This new human emergent infectious disease is dominated by severe lower respiratory illness and is aetiologically linked to a new coronavirus (SARS-CoV). METHODS: Pulmonary pathology and clinical correlates were investigated in seven patients who died of SARS in whom there was a strong epidemiological link. Investigations include a review of clinical features, morphological assessment, histochemical and immunohistochemical stainings, ultrastructural study, and virological investigations in postmortem tissue. RESULTS: Positive viral culture for coronavirus was detected in most premortem nasopharyngeal aspirate specimens (five of six) and postmortem lung tissues (two of seven). Viral particles, consistent with coronavirus, could be detected in lung pneumocytes in most of the patients. These features suggested that pneumocytes are probably the primary target of infection. The pathological features were dominated by diffuse alveolar damage, with the presence of multinucleated pneumocytes. Fibrogranulation tissue proliferation in small airways and airspaces (bronchiolitis obliterans organising pneumonia-like lesions) in subpleural locations was also seen in some patients. CONCLUSIONS: Viable SARS-CoV could be isolated from postmortem tissues. Postmortem examination allows tissue to be sampled for virological investigations and ultrastructural examination, and when coupled with the appropriate lung morphological changes, is valuable to confirm the diagnosis of SARS-CoV, particularly in clinically unapparent or suspicious but unconfirmed cases.


Asunto(s)
Coronavirus/aislamiento & purificación , Pulmón/patología , Síndrome Respiratorio Agudo Grave/patología , Adulto , Anciano , Anciano de 80 o más Años , División Celular , Núcleo Celular/patología , Células Cultivadas , Femenino , Humanos , Inmunohistoquímica/métodos , Pulmón/virología , Masculino , Microscopía Electrónica , Persona de Mediana Edad , Alveolos Pulmonares/patología , Síndrome Respiratorio Agudo Grave/virología
12.
Int J Rehabil Res ; 26(2): 137-40, 2003 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-12799608

RESUMEN

In this prospective study, we surveyed the pain experience of 40 participants during the in-patient rehabilitation period following traumatic spinal cord injury (SCI). Twenty-eight patients (70% of the study population) had musculoskeletal (MS) pain or neuropathic (NP) pain. Pain responded positively to physical therapy and analgesics. A numerical pain scale decreased from a mean of 6.36+/-1.7 on admission to 3.2+/-1.94 on discharge (P<0.001). Paraplegic patients were more likely to have MS pain (P=0.001) and NP pain (P=0.046). There was no relationship between completeness of injury, or spinal surgery, and type of pain encountered. There was also no significant difference in the modified Barthel index between patients with and without pain on admission and discharge. We conclude that pain is a common experience in SCI patients and that it can be reduced significantly by the end of in-patient rehabilitation.


Asunto(s)
Músculo Esquelético/fisiopatología , Dolor/etiología , Dolor/fisiopatología , Paraplejía/complicaciones , Traumatismos de la Médula Espinal/complicaciones , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Manejo del Dolor , Dimensión del Dolor , Paraplejía/fisiopatología , Paraplejía/rehabilitación , Estudios Prospectivos , Traumatismos de la Médula Espinal/fisiopatología , Traumatismos de la Médula Espinal/rehabilitación
13.
Int J Oral Maxillofac Surg ; 32(1): 104-6, 2003 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-12653245

RESUMEN

Human dirofilariasis is a helminthic zoonosis that is common in some parts of the world. A sporadic case of dirofilariasis affecting the buccal mucosa has been reported in a non-endemic area of southern China. Clinical findings, diagnosis, pathogenesis and management are discussed. This is the fourth case of human dirofilariasis involving the oral mucosa reported in world literature.


Asunto(s)
Dirofilariasis/diagnóstico , Enfermedades de la Boca/parasitología , Adulto , Animales , Diagnóstico Diferencial , Dirofilaria/clasificación , Dirofilariasis/patología , Femenino , Estudios de Seguimiento , Humanos , Enfermedades de la Boca/patología , Mucosa Bucal/parasitología , Mucosa Bucal/patología
14.
Singapore Med J ; 44(9): 491-5, 2003 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-14740781

RESUMEN

The ultrasonographical and computed tomography findings of a six-month-old female infant with haemangioendotheliomas of the liver and spleen are described. The splenic lesion had an unusual ultrasonographical appearance of a well-defined isoechoic mass with a hypoechoic rim. Diagnosis was confirmed by histological examination of the hepatic biopsy specimen. Hepatosplenic lesions in the first year of life may be due to a variety of pathological processes. It is important to include haemangioendotheliomas in the differential diagnosis of hepatosplenic masses in an infant.


Asunto(s)
Hemangioendotelioma/diagnóstico por imagen , Neoplasias Hepáticas/diagnóstico por imagen , Neoplasias del Bazo/diagnóstico por imagen , Femenino , Hemangioendotelioma/patología , Humanos , Lactante , Neoplasias Hepáticas/patología , Neoplasias del Bazo/patología , Tomografía Computarizada por Rayos X , Ultrasonografía
15.
Pediatr Surg Int ; 18(8): 730-2, 2002 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-12598975

RESUMEN

Adrenocortical carcinoma (ACC) is rare in children. Its presentation is usually related to hormonal activity of the tumour. We report a case of childhood ACC that presented as an acute abdomen due to tumour rupture. This is the first reported case of a ruptured ACC as a cause of paediatric acute abdomen.


Asunto(s)
Abdomen Agudo/etiología , Neoplasias de las Glándulas Suprarrenales/complicaciones , Carcinoma/complicaciones , Neoplasias de las Glándulas Suprarrenales/diagnóstico , Neoplasias de las Glándulas Suprarrenales/cirugía , Carcinoma/diagnóstico , Carcinoma/cirugía , Preescolar , Humanos , Masculino , Rotura Espontánea
16.
Org Lett ; 3(25): 3991-4, 2001 Dec 13.
Artículo en Inglés | MEDLINE | ID: mdl-11735567

RESUMEN

[reaction: see text] The addition reactions of various nucleophiles to a furyl aldehyde bearing a chiral boronate at the C-3 position furnished chromatographically separable diastereomers. The R diastereoselection was more favorable when no additive was added. Surprisingly, when lithium alkoxides were selected as additives, the S diastereoselection is superior instead. Further transformation of C-B bonds to C-C bonds was achieved by using standard Suzuki coupling conditions to give optically active 2,3-disubstituted furyl alcohols.


Asunto(s)
Alcoholes/síntesis química , Aldehídos/química , Ácidos Borónicos/química , Alcoholes/química , Cristalografía por Rayos X , Compuestos de Litio/química , Espectroscopía de Resonancia Magnética , Conformación Molecular , Estructura Molecular , Oxidación-Reducción , Estereoisomerismo
17.
Otolaryngol Head Neck Surg ; 125(5): 516-9, 2001 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-11700452

RESUMEN

OBJECTIVE: Five cases of head and neck fibromatosis were analyzed. The imaging and pathologic findings, surgical management, and clinical outcome were discussed. STUDY DESIGN AND SETTING: A retrospective study of 5 adult head and neck fibromatosis cases, evaluating long-term follow-up results of conservative treatment. RESULTS: The 5 patients (2 male and 3 female) ranged in age from 16 to 51 years. The lesion size ranged from 1 to 8 cm. Four cases had limited surgical resection; 1 case was followed only. One of the surgically treated cases had a recurrence that was irradiated. All patients were well for a follow-up period of 2 to 8 years. CONCLUSION: Aggressive excision of head and neck fibromatosis cannot be achieved easily. Vigilant follow-up with or without conservative surgical excision achieves good disease control. Low dose radiotherapy can be used for inoperable cases. SIGNIFICANCE: Judicious conservative treatment should be attempted for head and neck fibromatosis to achieve optimal functional preservation.


Asunto(s)
Fibroma , Neoplasias de Cabeza y Cuello , Adolescente , Adulto , Femenino , Fibroma/diagnóstico , Fibroma/patología , Fibroma/cirugía , Neoplasias de Cabeza y Cuello/diagnóstico , Neoplasias de Cabeza y Cuello/patología , Neoplasias de Cabeza y Cuello/cirugía , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Tomografía Computarizada por Rayos X
18.
Lasers Surg Med ; 29(3): 213-20, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11573222

RESUMEN

BACKGROUND AND OBJECTIVE: Hyper-osmotic chemical agents were used to study the effects of transient tissue scattering on the remitted fluorescence emission intensity from a target placed under a tissue sample. STUDY DESIGN/MATERIALS AND METHODS: A fluorescent film was placed underneath in vitro and in vivo samples of hamster skin, and the remitted fluorescent signal traveling to the tissue surface was monitored over time as the tissue was treated with an osmotically active agent. RESULTS: The detected fluorescent signal increased as the scattering in tissue samples was substantially reduced. The increase was greater for dimethyl sulfoxide than glucose or glycerol. It was not statistically different between in vivo skin and in vitro skin. CONCLUSION: The study shows how chemical agents can be used to improve the detected signal for a specific optical application. It could be useful in a number of optical therapeutic and diagnostic applications that can benefit from an increase in the penetration depth of light.


Asunto(s)
Dimetilsulfóxido/farmacocinética , Fluorescencia , Glucosa/farmacocinética , Glicerol/farmacocinética , Óptica y Fotónica , Ósmosis/fisiología , Fenómenos Fisiológicos de la Piel/efectos de los fármacos , Análisis de Varianza , Animales , Cricetinae , Técnicas In Vitro , Mesocricetus , Microscopía , Ratas , Ratas Sprague-Dawley , Dispersión de Radiación , Piel/efectos de los fármacos , Piel/patología
19.
Appl Environ Microbiol ; 67(9): 4186-91, 2001 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-11526022

RESUMEN

Campylobacter jejuni is a leading cause of bacterial gastroenteritis in humans, and contamination of poultry has been implicated in illness. The bacteria are fastidious in terms of their temperature requirements, being unable to grow below ca. 31 degrees C, but have been found to be physiologically active at lower temperatures and to tolerate exposure to low temperatures in a strain-dependent manner. In this study, 19 field isolates of C. jejuni (10 of clinical and 9 of poultry origin) were studied for their ability to tolerate prolonged exposure to low temperature (4 degrees C). Although substantial variability was found among different strains, clinical isolates tended to be significantly more likely to remain viable following cold exposure than poultry-derived strains. In contrast, the relative degree of tolerance of the bacteria to freezing at -20 degrees C and freeze-thawing was strain specific but independent of strain source (poultry versus clinical) and degree of cold (4 degrees C) tolerance.


Asunto(s)
Infecciones por Campylobacter/microbiología , Campylobacter jejuni/crecimiento & desarrollo , Pollos , Frío , Enfermedades de las Aves de Corral/microbiología , Animales , Infecciones por Campylobacter/veterinaria , Campylobacter jejuni/ultraestructura , Medios de Cultivo , Congelación , Humanos
20.
Head Neck ; 23(8): 625-8, 2001 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-11443744

RESUMEN

BACKGROUND: Lymphomas of T-cell and T/natural killer (T/NK)-cell types rarely involve the larynx. Early symptoms are subtle and nonspecific, and confirmation of the diagnosis is often difficult. Because of the rarity of this disease entity, the optimal management remains controversial. METHODS: The case records of patients with the diagnosis of lymphoma involving the larynx were retrospectively reviewed. Details of the pathologic condition, the difficulties in diagnosis, and treatment were presented. RESULTS: One case of T-cell lymphoma (Lennert's lymphoma subtype) and two cases of T/NK-cell lymphomas involving the larynx were presented. The diagnoses were all difficult to establish. All three patients received either multiagent chemotherapy or radiotherapy as the first-line treatment. They all died of the disease within 32 months of diagnosis. CONCLUSIONS: T- and T/NK-cell non-Hodgkin's lymphoma of the larynx is a diagnostic challenge for clinicians and pathologists. Conventional chemotherapy or radiotherapy has not been effective. A more aggressive therapy should be considered.


Asunto(s)
Células Asesinas Naturales , Neoplasias Laríngeas/diagnóstico , Linfoma de Células T/diagnóstico , Adulto , Resultado Fatal , Humanos , Neoplasias Laríngeas/patología , Linfoma de Células T/patología , Linfoma de Células T Periférico/diagnóstico , Linfoma de Células T Periférico/patología , Masculino , Persona de Mediana Edad , Pronóstico , Estudios Retrospectivos
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