RESUMEN

Polygalacturonase-inhibiting proteins (PGIPs) are plant cell wall proteins that specifically inhibit the activity of endopolygalacturonases (PGs) produced by fungi during the infection process. The interaction with PGIPs limits the destructive potential of PGs and may trigger plant defence responses through the release of elicitor active oligogalacturonides. In order to pinpoint the residues of PvPGIP2 from Phaseolus vulgaris involved in the interaction with PGs, we used site-directed mutagenesis to mutate the residues D131, D157 and D203, and tested for the inhibitory activity of the mutant proteins expressed in Pichia pastoris against Fusarium phyllophilum and Aspergillus niger PGs. Here, we report that mutation of these residues affects the inhibition capacity of PvPGIP2 against F. phyllophilum PG.

Asunto(s)

Fusarium/enzimología , Interacciones Huésped-Patógeno , Phaseolus/metabolismo , Proteínas de Plantas/metabolismo , Poligalacturonasa/antagonistas & inhibidores , Secuencia de Aminoácidos , Ácido Aspártico/metabolismo , Secuencia de Bases , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Phaseolus/genética , Phaseolus/microbiología , Proteínas de Plantas/genética , Poligalacturonasa/metabolismo

RESUMEN

We have identified, expressed and characterized two genes from Arabidopsis thaliana (AtPMEI-1 and AtPMEI-2) encoding functional inhibitors of pectin methylesterases. AtPMEI-1 and AtPMEI-2 are cell wall proteins sharing many features with the only pectin methylesterase inhibitor (PMEI) characterized so far from kiwi fruit. Both Arabidopsis proteins interact with and inhibit plant-derived pectin methylesterases (PMEs) but not microbial enzymes. The occurrence of functional PMEIs in Arabidopsis indicates that a mechanism of controlling pectin esterification by inhibition of endogenous PMEs is present in different plant species.

Asunto(s)

Arabidopsis/genética , Hidrolasas de Éster Carboxílico/antagonistas & inhibidores , Inhibidores Enzimáticos/metabolismo , Secuencia de Aminoácidos , Arabidopsis/enzimología , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Proteínas de Arabidopsis/farmacología , Secuencia de Bases , Pared Celular/metabolismo , Clonación Molecular , Secuencia Conservada , Cartilla de ADN , ADN de Plantas/genética , ADN de Plantas/aislamiento & purificación , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/farmacología , Genes de Plantas , Cinética , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Homología de Secuencia de Aminoácido

RESUMEN

Polygalacturonase-inhibiting proteins (PGIPs) are plant cell wall proteins that protect plants from fungal invasion. They interact with endopolygalacturonases secreted by phytopathogenic fungi, inhibit their enzymatic activity, and favor the accumulation of oligogalacturonides, which activate plant defense responses. PGIPs are members of the leucine-rich repeat (LRR) protein family that in plants play crucial roles in development, defense against pathogens, and recognition of beneficial microbes. Here we report the crystal structure at 1.7-A resolution of a PGIP from Phaseolus vulgaris. The structure is characterized by the presence of two beta-sheets instead of the single one originally predicted by modeling studies. The structure also reveals a negatively charged surface on the LRR concave face, likely involved in binding polygalacturonases. The structural information on PGIP provides a basis for designing more efficient inhibitors for plant protection.

Asunto(s)

Phaseolus/química , Proteínas de Plantas/química , Secuencia de Aminoácidos , Cristalografía por Rayos X , Inhibidores Enzimáticos/química , Hongos/enzimología , Hongos/patogenicidad , Genes de Plantas , Modelos Moleculares , Datos de Secuencia Molecular , Phaseolus/genética , Phaseolus/fisiología , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/genética , Proteínas de Plantas/fisiología , Poligalacturonasa/antagonistas & inhibidores , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Estructura Secundaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Homología de Secuencia de Aminoácido , Electricidad Estática

RESUMEN

To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes; among them, endopolygalacturonase (PG) catalyzes the fragmentation and solubilization of homogalacturonan. Polygalacturonase-inhibiting proteins (PGIPs), found in the cell wall of many plants, counteract fungal PGs by forming specific complexes with them. We report the crystal structure at 1.73 A resolution of PG from the phytopathogenic fungus Fusarium moniliforme (FmPG). The structure of FmPG was useful to study the mode of interaction of the enzyme with PGIP-2 from Phaseolus vulgaris. Several amino acids of FmPG were mutated, and their contribution to the formation of the complex with PGIP-2 was investigated by surface plasmon resonance. The residues Lys-269 and Arg-267, located inside the active site cleft, and His-188, at the edge of the active site cleft, are critical for the formation of the complex, which is consistent with the observed competitive inhibition of the enzyme played by PGIP-2. The replacement of His-188 with a proline or the insertion of a tryptophan after position 270, variations that both occur in plant PGs, interferes with the formation of the complex. We suggest that these variations are important structural requirements of plant PGs to prevent PGIP binding.

Asunto(s)

Proteínas de Plantas/metabolismo , Poligalacturonasa/metabolismo , Secuencia de Bases , Sitios de Unión , Cristalografía por Rayos X , Cartilla de ADN , Fusarium/enzimología , Modelos Moleculares , Mutagénesis Sitio-Dirigida , Poligalacturonasa/antagonistas & inhibidores , Poligalacturonasa/química , Poligalacturonasa/genética , Conformación Proteica , Resonancia por Plasmón de Superficie

RESUMEN

Polygalacturonase-inhibiting proteins (PGIPs) are extracellular plant proteins capable of inhibiting fungal endopolygalacturonases (PGs). Plants have evolved different PGIPs with specific recognition abilities against the many PGs produced by fungi. The genes encoding PGIPs are organized into families, and different members of each family may encode proteins with nearly identical characteristics but different specificities and regulation. PGIPs are typically induced by pathogen infection and stress-related signals. The recognition ability of PGIPs resides in their LRR (leucine-rich repeat) structure, where solvent-exposed residues in the beta-strand/beta-turn motifs of the LRRs are determinants of specificity. Manipulation of the primary structure of PGIPs is expected to generate more efficient PGIPs with novel recognition specificities to protect crop plants against pathogens.

Asunto(s)

Hongos/crecimiento & desarrollo , Magnoliopsida/genética , Enfermedades de las Plantas/genética , Proteínas de Plantas/genética , Secuencia de Aminoácidos , Pared Celular , Hongos/patogenicidad , Regulación de la Expresión Génica de las Plantas , Inmunidad Innata/genética , Proteínas Repetidas Ricas en Leucina , Magnoliopsida/enzimología , Magnoliopsida/microbiología , Datos de Secuencia Molecular , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/metabolismo , Poligalacturonasa/metabolismo , Proteínas/genética , Proteínas/fisiología , Homología de Secuencia de Aminoácido , Transducción de Señal

RESUMEN

A detailed analysis of the secondary structure has been carried out on the polygalacturonase-inhibiting protein (PGIP) from Phaseolus vulgaris, a leucine-rich repeat (LRR) protein present in the cell wall of many plants. Far-UV CD and infrared spectroscopies coupled to constrained secondary structure prediction methods indicated the presence of 12 alpha- and 12 beta-segments, thus allowing a schematic representation of three domains of the protein, namely, the central LRR region and the two cysteine-rich flanking domains. Peptides from endoproteinase-degraded PGIP were analyzed by mass spectrometry, and four disulfide bonds were identified. Mass spectrometric analysis in combination with glycosidase treatments revealed two N-linked oligosaccharides located on Asn 64 and Asn 141. The main structure resembled the typical complex plant N-glycan consisting of a core pentasaccharide beta1,2-xylosylated, carrying an alpha1,3-fucose linked to the innermost N-acetylglucosamine and one outer arm N-acetylglucosamine residue. The schematic representation of PGIP structural domains is discussed in the framework of the structure and function of LRR proteins.

Asunto(s)

Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/metabolismo , Leucina/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Poligalacturonasa/antagonistas & inhibidores , Procesamiento Proteico-Postraduccional , Secuencias Repetitivas de Aminoácido , Secuencia de Aminoácidos , Dicroismo Circular , Disulfuros/química , Fabaceae , Glicosilación , Datos de Secuencia Molecular , Plantas Medicinales , Estructura Secundaria de Proteína , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Espectroscopía Infrarroja por Transformada de Fourier

RESUMEN

A combination of surface plasmon resonance (SPR) and matrix-assisted laser-desorptionionization- time-of-flight mass spectrometry (MALDI-TOF-MS) was used to study the interaction between endopolygalacturonase (PG) from Fusarium moniliforme and a polygalacturonase-inhibiting protein (PGIP) from Phaseolus vulgaris. PG hydrolyses the homogalacturonan of the plant cell wall and is considered an important pathogenicity factor of many fungi. PGIP is a specific inhibitor of fungal PGs and is thought to be involved in plant defence against phytopathogenic fungi. SPR was used either to study the effect of the PG glycosylation on the formation of the complex with PGIP, and as a sensitive affinity capture of an interacting peptide from a mixture of PG fragments obtained by limited proteolysis. Mass spectrometry allowed to characterise the interacting peptide eluted from the sensor surface.

RESUMEN

alpha-1,4-Linked oligogalacturonides (OGs) inhibit auxin-regulated transcriptional activation of a rolB-beta-glucuronidase (GUS) gene fusion in tobacco (Nicotiana tabacum) leaf explants (D. Bellincampi, M. Cardarelli, D. Zaghi, G. Serino, G. Salvi, C. Gatz, F. Cervone, M. M. Altamura, P. Costantino, G. De Lorenzo [1996] Plant Cell 8: 477-487). In this paper we show that inhibition by OGs is very rapid, with a short lag time, and takes place even after rolB promoter activation has initiated. OGs also induce a transient and catalase-sensitive accumulation of H(2)O(2) in the leaf explant culture medium. OGs with a degree of polymerization from 12 to 15 are required for both the inhibition of the auxin-induced rolB-driven accumulation of GUS and the induction of H(2)O(2) accumulation(.) However, OG concentration for half-maximal induction of H(2)O(2) accumulation is approximately 3-fold higher than that for half-maximal inhibition of rolB promoter activity. The inhibition of rolB promoter activity is not influenced by the addition of catalase or superoxide dismutase, suggesting that H(2)O(2) and superoxide are not involved in this effect. A fungal oligo-beta-glucan elicitor induces extracellular H(2)O(2) accumulation at comparable or higher levels than those observed with OGs, but does not prevent the auxin-induced accumulation of GUS. We conclude that H(2)O(2) produced upon treatment with OGs is not involved in the inhibition of the auxin-induced expression of the rolB gene.

Asunto(s)

Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Ácidos Hexurónicos/farmacología , Peróxido de Hidrógeno/metabolismo , Ácidos Indolacéticos/fisiología , Nicotiana/genética , Oligosacáridos/farmacología , Proteínas Oncogénicas/genética , Plantas Tóxicas , beta-Glucosidasa/genética , Regulación de la Expresión Génica de las Plantas/fisiología , Hojas de la Planta/metabolismo

RESUMEN

A cDNA clone encoding a leucine-rich repeat (LRR) receptor-like protein kinase (LRPKm1) of Malus x domestica cv. Florina has been isolated using as a heterologous probe a cloned gene encoding a polygalacturonase-inhibiting protein (PGIP) of Phaseolus vulgaris L. A genomic clone containing the 5'-regulatory region and a 5' portion of the open reading frame of the LRPKm1 gene has also been isolated. An open reading frame of 2997 nt (999 amino acids) was present in the cDNA clone, encoding a receptor-like protein comprising a 21 amino acid signal peptide for secretion, a leucine zipper, 23 LRRs, a putative membrane-spanning region and a serine/threonine protein kinase domain. LRPKm1 shows homology to the A. thaliana receptor-like protein kinase RLK5 and, to a minor extent, to PGIP. The LRPKm1 region from +5 to +600 exhibits an alternative reading frame that encodes a product corresponding to a proline-rich protein fragment homologous to several hydroxyproline-rich proteins. Southern blot analysis showed that LRPKm1 belongs to a multigene family and that there is length polymorphism of the hybridizing restriction fragments among different M. x domestica cultivars. Northern blot analysis was carried out on mRNA extracted from infected leaves of either cv. Florina (resistant to Venturia inaequalis) or cv. Golden Delicious (susceptible to V. inaequalis), and from tissues treated with salicylic acid. A 3500 bp transcript hybridizing at high stringency with the LRPKm1 cDNA accumulated in response to infection or salicylic acid treatment. Transcript accumulation was more intense in the incompatible interaction than in the compatible one. The possible involvement of this receptor-like protein kinase in resistance of apple to phytopathogenic fungi is discussed.

Asunto(s)

Antifúngicos/farmacología , Frutas/genética , Enfermedades de las Plantas/microbiología , Proteínas/genética , Proteínas Tirosina Quinasas Receptoras/genética , Ácido Salicílico/farmacología , Secuencia de Aminoácidos , Ascomicetos/crecimiento & desarrollo , Secuencia de Bases , Northern Blotting , ADN Complementario/química , ADN Complementario/genética , ADN Complementario/aislamiento & purificación , ADN de Plantas/química , ADN de Plantas/genética , ADN de Plantas/aislamiento & purificación , Frutas/enzimología , Frutas/microbiología , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Genes de Plantas/genética , Proteínas Repetidas Ricas en Leucina , Datos de Secuencia Molecular , Familia de Multigenes/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteínas Serina-Treonina Quinasas , ARN Mensajero/efectos de los fármacos , ARN Mensajero/genética , ARN Mensajero/metabolismo , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Transcripción Genética/efectos de los fármacos

RESUMEN

Endo-polygalacturonases catalyze the fragmentation and solubilization of the homogalacturonan of the plant cell wall. These enzymes are extracellularly targeted glycoproteins produced by a number of organisms such as fungi, bacteria and plants, and are involved in both pathological and physiological processes. Single crystals of the endo-polygalacturonase from the phytopathogenic fungus Fusarium moniliforme were obtained by the vapour-diffusion method at 294 K. The starting material as well as the crystal consist of three forms with different degrees of glycosylation. The crystals belong to the orthorhombic space group P212121 and diffract to 1.9 A resolution on a synchrotron-radiation source under cryocooling conditions.

Asunto(s)

Fusarium/enzimología , Poligalacturonasa/química , Cristalización , Cristalografía por Rayos X , Congelación , Conformación Proteica , Proteínas Recombinantes/química

RESUMEN

Two members of the pgip gene family (pgip-1 and pgip-2) of Phaseolus vulgaris L. were expressed separately in Nicotiana benthamiana and the ligand specificity of their products was analysed by surface plasmon resonance (SPR). Polygalacturonase-inhibiting protein-1 (PGIP-1) was unable to interact with PG from Fusarium moniliforme and interacted with PG from Aspergillus niger; PGIP-2 interacted with both PGs. Only eight amino acid variations distinguish the two proteins: five of them are confined within the beta-sheet/beta-turn structure and two of them are contiguous to this region. By site-directed mutagenesis, each of the variant amino acids of PGIP-2 was replaced with the corresponding amino acid of PGIP-1, in a loss-of-function approach. The mutated PGIP-2s were expressed individually in N.benthamiana, purified and subjected to SPR analysis. Each single mutation caused a decrease in affinity for PG from F.moniliforme; residue Q253 made a major contribution, and its replacement with a lysine led to a dramatic reduction in the binding energy of the complex. Conversely, in a gain-of-function approach, amino acid K253 of PGIP-1 was mutated into the corresponding amino acid of PGIP-2, a glutamine. With this single mutation, PGIP-1 acquired the ability to interact with F.moniliforme PG.

Asunto(s)

Proteínas de Plantas/química , Poligalacturonasa/antagonistas & inhibidores , Secuencia de Aminoácidos , Secuencia de Bases , ADN Complementario/genética , Fabaceae/genética , Fusarium/enzimología , Biblioteca de Genes , Modelos Moleculares , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Proteínas de Plantas/genética , Plantas Medicinales , Plantas Tóxicas , Estructura Secundaria de Proteína , Proteínas Recombinantes/química , Secuencias Repetitivas de Aminoácido , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Nicotiana/genética

RESUMEN

Polygalacturonase-inhibiting proteins (PGIPs), leucine-rich repeat (LRR) proteins evolutionarily related to several plant resistance genes, bind to and regulate the action of fungal endopolygalacturonases. In Phaseolus vulgaris L., PGIPs are encoded by a gene family comprising at least five members. As a start for a systematic analysis of the regulation of the pgip family, we have analysed the ability of the promoter of the bean gene pgip-1 to direct expression of beta-glucuronidase (GUS) in transfected tobacco protoplasts, microbombarded bean and tobacco leaves, and transgenic tobacco plants. In protoplasts, the pgip-1 gene region from nucleotide (nt) -2004 to nt +27 directed a level of expression that was as high as that directed by the cauliflower mosaic virus (CaMV) 35S promoter and could not be further induced by elicitor treatment; alteration of the region immediately following the TATAA sequence at nt -29 abolished expression. Upon stable integration into tobacco plants of the pgip-1 promoter-GUS construct, as well as of a -394 deletion, expression was detected for both constructs mainly in the stigma and, to a lesser extent, in the anthers and in the conductive vascular tissue. The promoter responded to wounding but not to oligogalacturonides, fungal glucan, salicylic acid, cryptogein, or pathogen infection. This expression pattern does not mirror that of the whole pgip gene family.

Asunto(s)

Inhibidores Enzimáticos , Fabaceae/genética , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Proteínas de Plantas/genética , Plantas Medicinales , Poligalacturonasa/antagonistas & inhibidores , Regiones Promotoras Genéticas , Fusión Artificial Génica , Fabaceae/microbiología , Glucuronidasa/genética , Phytophthora/fisiología , Plantas Modificadas Genéticamente , Plantas Tóxicas , Pseudomonas/fisiología , Nicotiana

RESUMEN

The filamentous fungus Cochliobolus carbonum produces endo-alpha 1,4-polygalacturonase (endoPG), exo-alpha 1,4-polygalacturonase (exoPG), and pectin methylesterase when grown in culture on pectin. Residual activity in a pgn1 mutant (lacking endoPG) was due to exoPG activity, and the responsible protein has now been purified. After chemical deglycosylation, the molecular mass of the purified protein decreased from greater than 60 to 45 kDa. The gene that encodes exoPG, PGX1, was isolated with PCR primers based on peptide sequences from the protein. The product of PGX1, Pgx1p, has a predicted molecular mass of 48 kDa, 12 potential N-glycosylation sites, and 61% amino acid identity to an exoPG from the saprophytic fungus Aspergillus tubingensis. Strains of C. carbonum mutated in PGX1 were constructed by targeted gene disruption and by gene replacement. Growth of pgx1 mutant strains on pectin was reduced by ca. 20%, and they were still pathogenic on maize. A double pgn1/pgx1 mutant strain was constructed by crossing. The double mutant grew as well as the pgx1 single mutant on pectin and was still pathogenic despite having less than 1% of total wild-type PG activity. Double mutants retained a small amount of PG activity with the same cation-exchange retention time as Pgn1p and also pectin methylesterase and a PG activity associated with the mycelium. Continued growth of the pgn1/pgx1 mutant on pectin could be due to one or more of these residual activities.

Asunto(s)

Ascomicetos/enzimología , Ascomicetos/genética , Mutación , Poligalacturonasa/genética , Secuencia de Aminoácidos , Ascomicetos/crecimiento & desarrollo , Secuencia de Bases , Clonación Molecular , ADN de Hongos/genética , Expresión Génica , Marcación de Gen , Genes Fúngicos , Glicósido Hidrolasas/genética , Glicósido Hidrolasas/aislamiento & purificación , Datos de Secuencia Molecular , Pectinas/metabolismo , Fenotipo , Poligalacturonasa/aislamiento & purificación , Mapeo Restrictivo , Virulencia/genética , Zea mays/microbiología

RESUMEN

The pgip-1 gene of Phaseolus vulgaris, encoding a polygalacturonase-inhibiting protein (PGIP), PGIP-1 (P. Toubart, A. Desiderio, G. Salvi, F. Cervone, L. Daroda, G. De Lorenzo, C. Bergmann, A. G. Darvill, and P. Albersheim, Plant J. 2:367-373, 1992), was expressed under control of the cauliflower mosaic virus 35S promoter in tomato plants via Agrobacterium tumefaciens-mediated transformation. Transgenic tomato plants with different expression levels of PGIP-1 were used in infection experiments with the pathogenic fungi Fusarium oxysporum f. sp. lycopersici, Botrytis cinerea, and Alternaria solani. No evident enhanced resistance, compared with the resistance of untransformed plants, was observed. The pgip-1 gene was also transiently expressed in Nicotiana benthamiana with potato virus X (PVX) as a vector. PGIP-1 purified from transgenic tomatoes and PGIP-1 in crude protein extracts of PVX-infected N. benthamiana plants were tested with several fungal polygalacturonases (PGs). PGIP-1 from both plant sources exhibited a specificity different from that of PGIP purified from P. vulgaris (bulk bean PGIP). Notably, PGIP-1 was unable to interact with a homogeneous PG from Fusarium moniliforme, as determined by surface plasmon resonance analysis, while the bulk bean PGIP interacted with and inhibited this enzyme. Moreover, PGIP-1 expressed in tomato and N. benthamiana had only a limited capacity to inhibit crude PG preparations from F. oxysporum f. sp. lycopersici, B. cinerea, and A. solani. Differential affinity chromatography was used to separate PGIP proteins present in P. vulgaris extracts. A PGIP-A with specificity similar to that of PGIP-1 was separated from a PGIP-B able to interact with both Aspergillus niger and F. moniliforme PGs. Our data show that PGIPs with different specificities are expressed in P. vulgaris and that the high-level expression of one member (pgip-1) of the PGIP gene family in transgenic plants is not sufficient to confer general, enhanced resistance to fungi.

Asunto(s)

Fabaceae/genética , Proteínas de Plantas/genética , Plantas Medicinales , Inhibidores Enzimáticos , Fabaceae/microbiología , Solanum lycopersicum/genética , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , Especificidad por Sustrato

RESUMEN

The sequence encoding the endopolygalacturonase (PG) of Fusarium moniliforme was cloned into the E. coli/yeast shuttle vector Yepsec1 for secretion in yeast. The recombinant plasmid (pCC6) was used to transform Saccharomyces cerevisiae strain S150-2B; transformed yeast cells were able to secrete PG activity into the culture medium. The enzyme (wtY-PG) was purified, characterized, and shown to possess biochemical properties similar to those of the PG purified from F. moniliforme. The wtY-PG was able to macerate potato medullary tissue disks and was inhibited by the polygalacturonase-inhibiting protein (PGIP) purified from Phaseolus vulgaris. The sequence encoding PG in pCC6 was subjected to site-directed mutagenesis. Three residues in a region highly conserved in all the sequences known to encode PGs were separately mutated: His 234 was mutated into Lys (H 234-->K), and Ser 237 and Ser 240 into Gly (S 237-->G and S 240-->G). Each of the mutated sequences was used to transform S. cerevisiae and the mutated enzymes were purified and characterized. Replacement of His 234 with Lys abolished the enzymatic activity, confirming the biochemical evidence that a His residue is critical for enzyme activity. Replacement of either Ser 237 or Ser 240 with Gly reduced the enzymatic activity to 48% and 6%, respectively, of the wtY-PG. When applied to potato medullary tissue, F. moniliforme PG and wtY-PG caused comparable maceration, while the variant PGs exhibited a limited (S 234-->G and S 240-->G) or null (H 234-->K) macerating activity. The interaction between the variant enzymes and the P. vulgaris PGIP was investigated using a biosensor based on surface plasmon resonance (BIAlite). The three variant enzymes were still able to interact and bind to PGIP with association constants comparable to that of the wild type enzyme.

Asunto(s)

Fusarium/enzimología , Histidina , Poligalacturonasa/metabolismo , Secuencia de Aminoácidos , Secuencia de Bases , Sitios de Unión , Clonación Molecular , Secuencia Conservada , Cartilla de ADN , Inhibidores Enzimáticos/metabolismo , Fabaceae , Mutagénesis Sitio-Dirigida , Proteínas de Plantas/metabolismo , Plantas Medicinales , Poligalacturonasa/química , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Saccharomyces cerevisiae

RESUMEN

Oligogalacturonides elicit several defense responses and regulate different aspects of growth and development in plants. Many of the development-related effects of oligogalacturonides appear to be amenable to an auxin antagonist activity of these oligosaccharins. To clarify the role of oligogalacturonides in antagonizing auxin, we analyzed their effect on root formation in leaf explants of tobacco harboring the plant oncogene rolB. We show here that oligogalacturonides are capable of inhibiting root morphogenesis driven by rolB in transgenic leaf explants when this process requires exogenous auxin. Because rolB expression is induced by auxin and dramatically alters the response to this hormone in transformed plant cells, the inhibiting effect of oligogalacturonides could be exerted on the induction of rolB and/or at some other auxin-requiring step(s) in rhizogenesis. We show that oligogalacturonides antagonize auxin primarily because they strongly inhibit auxin-regulated transcriptional activation of a rolB-[beta]-glucuronidase gene fusion in both leaf explants and cultured leaf protoplasts. In contrast, oligogalacturonides do not inhibit rhizogenesis when rolB transcriptional activation is made independent of auxin, as shown by the lack of inhibition of root formation in leaf explants containing rolB driven by a tetracycline-inducible promoter.

RESUMEN

Polygalacturonase-inhibiting protein (PGIP) is a cell wall-associated protein that specifically binds to and inhibits the activity of fungal endopolygalacturonases. The Phaseolus vulgaris gene encoding PGIP has been cloned and characterized. Using a fragment of the cloned pgip gene as a probe in Northern blot experiments, it is demonstrated that the pgip mRNA accumulates in suspension-cultured bean cells following addition of elicitor-active oligogalacturonides or fungal glucan to the medium. Rabbit polyclonal antibodies specific for PGIP were generated against a synthetic peptide designed from the N-terminal region of PGIP; the antigenicity of the peptide was enhanced by coupling to KLH. Using the antibodies and the cloned pgip gene fragment as probes in Western and Northern blot experiments, respectively, it is shown that the levels of PGIP and its mRNA are increased in P. vulgaris hypocotyls in response to wounding or treatment with salicylic acid. Using gold-labeled goat-anti-rabbit secondary antibodies in EM studies, it has also been demonstrated that, in bean hypocotyls infected with Colletotrichum lindemuthianum, the level of PGIP preferentially increases in those cells immediately surrounding the infection site. The data support the hypothesis that synthesis of PGIP constitutes an active defense mechanism of plants that is elicited by signal molecules known to induce plant defense genes.

Asunto(s)

Fabaceae/metabolismo , Enfermedades de las Plantas , Proteínas de Plantas/metabolismo , Plantas Medicinales , Secuencia de Aminoácidos , Especificidad de Anticuerpos , Fabaceae/microbiología , Fabaceae/ultraestructura , Microscopía Electrónica , Hongos Mitospóricos/ultraestructura , Datos de Secuencia Molecular , Proteínas de Plantas/biosíntesis , Proteínas de Plantas/genética , ARN Mensajero/biosíntesis , Salicilatos/farmacología , Ácido Salicílico

Asunto(s)

Comunicación Celular , Oligosacáridos/metabolismo , Proteínas de Plantas/metabolismo , Poligalacturonasa/metabolismo , Secuencia de Aminoácidos , Ácidos Hexurónicos/metabolismo , Datos de Secuencia Molecular , Oligosacáridos/química , Proteínas de Plantas/química , Poligalacturonasa/antagonistas & inhibidores , Conformación Proteica , Transducción de Señal

Asunto(s)

Oligosacáridos/metabolismo , Fenómenos Fisiológicos de las Plantas , Pared Celular/metabolismo , Glucano Endo-1,3-beta-D-Glucosidasa/metabolismo , Interacciones Huésped-Parásitos , Phytophthora/fisiología , Desarrollo de la Planta , Extractos Vegetales/biosíntesis , Proteínas de Plantas/metabolismo , Plantas/microbiología , Poligalacturonasa/antagonistas & inhibidores , Sesquiterpenos , Terpenos , Fitoalexinas

RESUMEN

Polygalacturonase-inhibiting protein (PGIP) is a cell wall protein which inhibits fungalendopolygalacturonases. A small gene family encodesPGIP in the genome of common bean, as indicated by Southernblot experiments performed at high-stringency conditions. Southern-blot analysis of DNA extracted from different cultivars ofPhaseolus vulgaris and fromPhaseolus coccineus showed length polymorphism of the hybridizing restriction fragments. The cytological localization of thePGIP genes was determined in polytene chromosomes of theP. vulgaris embryo suspensor cells. In-situ hybridization experiments using the clonedPGIP gene revealed labelling over a single region of the pericentromeric heterochromatin of chromosome pair X, next to the euchromatin, suggesting thatPGIP gene family may be clustered in one chromosomal region.