RESUMEN

Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.

Asunto(s)

Cronobacter/clasificación , Tipificación Molecular/métodos , Análisis del Polimorfismo de Longitud de Fragmentos Amplificados , Animales , Cronobacter/genética , Cronobacter/aislamiento & purificación , Cronobacter/metabolismo , Cronobacter sakazakii/clasificación , Cronobacter sakazakii/genética , Cronobacter sakazakii/aislamiento & purificación , Cronobacter sakazakii/metabolismo , Enzimas de Restricción del ADN , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Electroforesis en Gel de Campo Pulsado , Infecciones por Enterobacteriaceae/diagnóstico , Infecciones por Enterobacteriaceae/microbiología , Alimentos en Conserva/microbiología , Enfermedades Transmitidas por los Alimentos/diagnóstico , Enfermedades Transmitidas por los Alimentos/microbiología , Humanos , Leche/microbiología , Reproducibilidad de los Resultados , Vibrio cholerae/clasificación , Vibrio cholerae/genética , Vibrio cholerae/aislamiento & purificación , Vibrio cholerae/metabolismo , Yersinia pestis/clasificación , Yersinia pestis/genética , Yersinia pestis/aislamiento & purificación , Yersinia pestis/metabolismo