Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 33
Filtrar
Más filtros











Intervalo de año de publicación
2.
Clin Transl Oncol ; 20(8): 1072-1079, 2018 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-29368144

RESUMEN

BACKGROUND: Immunotherapy increases overall response rate (ORR) and overall survival (OS) in patients with non-small-cell lung cancer (NSCLC). Prognostic and predictive factors are a high need. PATIENTS AND METHODS: Retrospective review of NSCLC patients treated with nivolumab was performed. Analyzed variables included age, sex, stage, performance status (PS), location of metastases, presence of tumour-related symptoms and comorbidities, number of metastasis locations, previous chemotherapy, anti-angiogenic and radiotherapy treatments, and analytical data from the standard blood count and biochemistry. RESULTS: A total of 175 patients were included. Median age was 61.5 years, 73.1% were men, 77.7% were ECOG-PS 0-1, and 86.7% were included with stage IV disease. Histology was non-squamous in 77.1%. Sixty-five received nivolumab in second line (37.1%). Thirty-eight patients had brain metastasis (22%), and 39 (22.3%) liver metastasis and 126 (72%) had more than one metastatic location. The ORR was 15.7% with median Progression free survival (PFS) 2.8 months and median OS 5.81 months. Stage III vs IV and time since the beginning of the previous line of treatment ≥ 6 vs < 6 months were associated with better response. PS 2, time since the previous line of treatment < 6 vs ≥ 6 months, and more than one metastatic location were independently associated with shorter OS in multivariable analysis (7.8 vs 2.7 months, 11.2 vs 4.6 months, and 9.4 vs 5.1 month). Finally, time since the previous treatment < 6 vs ≥ 6 months and more than one metastatic location were independently associated with shorter PFS in multivariable analysis (4.3 vs 2.3 months and 4.7 vs 2.3 months). CONCLUSION: Poor PS, short period of time since the previous treatment, and more than one metastatic location were associated with poorer prognostic.


Asunto(s)
Anticuerpos Monoclonales/uso terapéutico , Antineoplásicos/uso terapéutico , Carcinoma de Pulmón de Células no Pequeñas/secundario , Carcinoma de Células Escamosas/secundario , Neoplasias Pulmonares/patología , Recurrencia Local de Neoplasia/patología , Anciano , Carcinoma de Pulmón de Células no Pequeñas/tratamiento farmacológico , Carcinoma de Células Escamosas/tratamiento farmacológico , Femenino , Estudios de Seguimiento , Humanos , Neoplasias Pulmonares/tratamiento farmacológico , Masculino , Persona de Mediana Edad , Metástasis de la Neoplasia , Recurrencia Local de Neoplasia/tratamiento farmacológico , Nivolumab , Pronóstico , Estudios Retrospectivos , Tasa de Supervivencia
3.
Gene Ther ; 22(6): 449-57, 2015 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-25789461

RESUMEN

Mesenchymal stromal cells (MSCs) are cardioprotective in acute myocardial infarction (AMI). Besides, we have shown that intramyocardial injection of plasmid-VEGF(165) (pVEGF) in ovine AMI reduces infarct size and improves left ventricular (LV) function. We thus hypothesized that MSCs overexpressing VEGF(165) (MSCs-pVEGF) would afford greater cardioprotection than non-modified MSCs or pVEGF alone. Sheep underwent an anteroapical AMI and, 1 week later, received intramyocardial MSCs-pVEGF in the infarct border. One month post treatment, infarct size (magnetic resonance) decreased by 31% vs pre-treatment. Of note, myocardial salvage occurred predominantly at the subendocardium, the myocardial region displaying the largest contribution to systolic performance. Consistently, LV ejection fraction recovered to almost its baseline value because of marked decrease in end-systolic volume. None of these effects were observed in sheep receiving non-transfected MSCs or pVEGF. Although myocardial retention of MSCs decreased steeply over time, the treatment induced significant capillary and arteriolar proliferation, which reduced subendocardial fibrosis. We conclude that in ovine AMI, allogeneic VEGF-overexpressing MSCs induce subendocardial myocardium salvage through microvascular proliferation, reducing infarct size and improving LV function more than non-transfected MSCs or the naked plasmid. Importantly, the use of a plasmid rather than a virus allows for repeated treatments, likely needed in ischemic heart disease.


Asunto(s)
Células Madre Mesenquimatosas/metabolismo , Infarto del Miocardio/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismo , Animales , Línea Celular , Humanos , Espectroscopía de Resonancia Magnética/métodos , Masculino , Infarto del Miocardio/fisiopatología , Miocardio/patología , Plásmidos/metabolismo , Ovinos , Función Ventricular Izquierda
6.
J Inorg Biochem ; 99(3): 828-40, 2005 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-15708805

RESUMEN

myo-Inositol hexakisphosphate (InsP6) is an ubiquitous and abundant molecule in the cytosol and nucleus of eukaryotic cells whose biological functions are incompletely known. A major hurdle for studying the biology of InsP6 has been a deficiency of a full understanding of the chemistry of its interaction with divalent and trivalent cations. This deficiency has limited our appreciation of how it remains in solution within cells, and the likely degree to which it might interact in vivo with physiologically important cations such as Ca2+ and Fe3+. We report here the initial part of the description of the InsP6-multivalent cation chemistry, including its solution equilibria studied by high resolution potentiometry and (for the Fe(III)/Fe(II) couple) cyclic voltammetry. InsP6 forms anionic complexes of high affinities and 1:1 stoichiometry with Mg(II), Ca(II), Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II). Of particular importance is the observation that, in the exceptional case of Mg(II), InsP6 forms the species [Mg5(H2L)] (L representing fully deprotonated InsP6); this soluble neutral species is predicted to be the predominant form of InsP6 under nuclear or cytosolic conditions in animal cells. Contrary to previous suggestions, InsP6 is predicted not to interact with cytosolic calcium even when calcium is increased during signalling events. In vitro, InsP6 also forms high affinity 1:1 complexes with Fe(III) and Al(III). However, our data predict that in the biological context of excess free Mg(II), neither Fe(III) nor Fe(II) are complexed by InsP6.


Asunto(s)
Membrana Celular/metabolismo , Núcleo Celular/metabolismo , Citosol/metabolismo , Fosfatos de Inositol/química , Magnesio/química , Animales , Unión Competitiva , Calcio/química , Cationes , Electroquímica , Concentración de Iones de Hidrógeno , Fosfatos de Inositol/metabolismo , Hierro/química , Oxidación-Reducción , Valor Predictivo de las Pruebas , Protones , Soluciones
7.
Genetica ; 121(3): 219-28, 2004 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-15521420

RESUMEN

In seven mammalian species, including man, the position and number of nucleoli in pachytene spermatocyte nuclei were studied from electron microscope (EM) nuclear sections or bivalent microspreads. The number and position of the nucleolar organiser regions (NORs) in mitotic and meiotic chromosomes were also analysed, using silver staining techniques and in situ hybridisation protocols. The general organisation of pachytene spermatocyte nucleoli was almost the same, with only minor morphological differences between species. The terminal NORs of Thylamys elegans (Didelphoidea, Marsupialia), Dromiciops gliroides (Microbiotheridae, Marsupialia), Phyllotys osgoodi (Rodentia, Muridae) and man, always gave rise to peripheral nucleoli in the spermatocyte nucleus. In turn, the intercalated NORs from Octodon degus, Ctenomys opimus (Rodentia, Octodontidae) and Chinchilla lanigera (Rodentia, Cavidae), gave rise to central nucleoli. In species with a single nucleolar bivalent, just one nucleolus is formed, while in those with multiple nucleolar bivalents a variable number of nucleoli are formed by association of different nucleolar bivalents or NORs that occupy the same nuclear peripheral space (Phyllotis and man). It can be concluded that the position of each nucleolus within the spermatocyte nucleus is mainly dependent upon: (1) the position of the NOR in the nucleolar bivalent synaptonemal complex (SC), (2) the nuclear pathway of the nucleolar bivalent SC, being both telomeric ends attached to the nuclear envelope, and (3) the association between nucleolar bivalents by means of their NOR-nucleolar domains that occupy the same nuclear space. Thus, the distribution of nucleoli within the nuclear space of spermatocytes is non-random and it is consistent with the existence of a species-specific meiotic nuclear architecture.


Asunto(s)
Nucléolo Celular , Cromosomas de los Mamíferos/ultraestructura , Mamíferos/genética , Región Organizadora del Nucléolo/ultraestructura , Espermatocitos/citología , Animales , Humanos , Masculino , Microscopía Electrónica , Profase/genética , Tinción con Nitrato de Plata , Tritio
8.
J Colloid Interface Sci ; 249(2): 366-71, 2002 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-16290610

RESUMEN

Three cationic complexes containing the [Re((V))O](3+) core (general formula [ReO(dien-H)(aa)](+), dien=diethylenetriamine, aa=glycine, alanine, valine) were studied on polycrystalline Au electrodes employing cyclic voltammetry techniques. The electrochemical behavior of the amino acids (aa) was also evaluated. Experiments were performed at pH 7.0 aqueous solutions at room temperature. The voltammogram of the complex showed current contributions related to the [Re((VI))O](4+)/[Re((V))O](3+) redox couple, the counterion, and the amino acid ligand.


Asunto(s)
Aminoácidos/química , Electroquímica , Oxidación-Reducción
9.
Rev. argent. urol. [1990] ; 66(2): 88-91, abr.-jun. 2001. ilus
Artículo en Español | BINACIS | ID: bin-8511

RESUMEN

El motivo de esta presentación es actualizar un método terapéutico infrecuente utilizado en la reparación de las lesiones uretyralesþ, la transureterostomía término-terminal contralateral (cruzada) y trasmiteir nuestra experiencia con la comunicación de un caso(AU)


Asunto(s)
Humanos , Masculino , Adulto , Ureterostomía , Uréter/cirugía , Uréter/patología , Derivación Urinaria/métodos
10.
Rev. argent. urol. (1990) ; 66(2): 88-91, abr.-jun. 2001. ilus
Artículo en Español | LILACS | ID: lil-307025

RESUMEN

El motivo de esta presentación es actualizar un método terapéutico infrecuente utilizado en la reparación de las lesiones uretyralesç, la transureterostomía término-terminal contralateral (cruzada) y trasmiteir nuestra experiencia con la comunicación de un caso


Asunto(s)
Humanos , Masculino , Adulto , Derivación Urinaria/métodos , Uréter , Ureterostomía
11.
J Colloid Interface Sci ; 236(1): 104-107, 2001 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-11254334

RESUMEN

The electrochemical behavior of trans-[Re((V))O(2)(en)(2)]I and trans-[Re((V))O(2) (en)(2)]ClO(4) (en=ethylenediamine) complexes was studied by cyclic voltammetry on Au electrodes. Experiments were performed in aqueous solutions at pH 7.0 and at room temperature. The complex voltammogram was characterized by Re-containing species, assigned to the [Re((V))O(2)(en)(2)](+)/[Re((IV))O(2)(en)(2)] couple, and I-containing species. To overcome I interference, the electrochemical response of Re complexes was segregated by performing a reductive desorption of adsorbed I from Au. Copyright 2001 Academic Press.

12.
Rev Med Chil ; 125(11): 1357-60, 1997 Nov.
Artículo en Español | MEDLINE | ID: mdl-9609058

RESUMEN

Several disease have been associated with hepatitis C virus infections, including rheumatologic, hematologic and neoplastic disorders. We report two women, aged 57 and 39 years old whom the initial presentation of hepatitis C virus infection was an arthritis resembling rheumatoid arthritis. Laboratory work up revealed abnormal liver function tests, stimulating the search for hepatitis C virus infection, having both patients positive ELISA tests. Detection of this agent is extremely important when selecting a therapy for the articular disease, since several drugs used in the treatment of rheumatic disorders are potentially hepatotoxic and immunosuppression is risky in the setting of a viral hepatitis.


Asunto(s)
Artritis Infecciosa/etiología , Hepatitis C/complicaciones , Adulto , Femenino , Hepatitis C/diagnóstico , Humanos , Persona de Mediana Edad
13.
J Am Coll Cardiol ; 26(2): 313-8, 1995 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-7608429

RESUMEN

OBJECTIVES: This study was designed to test the hypothesis that low molecular weight heparin may lessen the severity of ischemic events in patients with unstable angina. BACKGROUND: Unstable angina is a thrombotic process that requires intensive medical treatment. Although current treatments can reduce the number of complications, serious bleeding continues to occur. Nadroparin calcium, a low molecular weight heparin, seems to be a safe therapeutic agent that does not require laboratory monitoring. METHODS: A total of 219 patients with unstable angina entered the study at a mean time of 6.17 h after the last episode of rest pain. Patients were randomized to receive aspirin (200 mg/day [group A]), aspirin plus regular heparin (400 IU/kg body weight per day intravenously and titered by activated partial thromboplastin time [group B]) and aspirin plus low molecular weight heparin (214 UIC/kg anti-Xa twice daily subcutaneously [group C]). The major end points determined for the in-hospital period were 1) recurrent angina, 2) myocardial infarction, 3) urgent revascularization, 4) major bleeding, and 5) death. Minor end points were 1) silent myocardial ischemia, and 2) minor bleeding. Event rates were tested by chi-square analysis. RESULTS: Recurrent angina occurred in 37%, 44% and 21% of patients in groups A, B and C, respectively, and was significantly less frequent in group C than in either group A (odds ratio 2.26, 95% confidence interval [CI] 1 to 5.18, p = 0.03) or group B (odds ratio, 3.07, 95% CI 1.36 to 7.00, p = 0.002). Nonfatal myocardial infarction was present in seven patients in group A, four in group B and none in group C (group B vs. A, p = 0.5; group C vs. A, p = 0.01). Urgent revascularization was performed in nine patients in group A, seven in group B and one in group C (C vs. A, p = 0.01). Two episodes of major bleeding occurred in group B. Silent myocardial ischemia was present in 38%, 41% and 25% of patients in groups A, B and C, respectively, and was significantly less frequent in group C than group B (odds ratio 2.12, 95% CI 0.97 to 4.69, p = 0.04). Minor bleeding was detected in 10 patients in group B, 1 patient in group C (B vs. C, p = 0.01) and no patient in group A (A vs. B, p = 0.003). CONCLUSIONS: In this study, treatment with aspirin plus a high dose of low molecular weight heparin during the acute phase of unstable angina was significantly better than treatment with aspirin alone or aspirin plus regular heparin.


Asunto(s)
Angina Inestable/tratamiento farmacológico , Aspirina/uso terapéutico , Heparina de Bajo-Peso-Molecular/uso terapéutico , Heparina/uso terapéutico , Isquemia Miocárdica/tratamiento farmacológico , Adulto , Anciano , Angina Inestable/prevención & control , Aspirina/efectos adversos , Distribución de Chi-Cuadrado , Quimioterapia Combinada , Femenino , Hemorragia/inducido químicamente , Heparina/efectos adversos , Heparina de Bajo-Peso-Molecular/efectos adversos , Humanos , Masculino , Persona de Mediana Edad , Infarto del Miocardio/prevención & control , Isquemia Miocárdica/prevención & control , Oportunidad Relativa , Estudios Prospectivos , Recurrencia , Método Simple Ciego , Resultado del Tratamiento
14.
Am J Cardiol ; 76(3): 121-4, 1995 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-7611144

RESUMEN

Ischemic electrocardiographic changes were recorded within 2 hours of admission using a 12-lead electrocardiographic continuous monitor with a 20-second scanning interval and an alarm mode for asymptomatic events. Blood samples were obtained at admission and at the moment of asymptomatic events (group A). In the other patients who did not develop ischemia, a second blood sample was taken 12 hours later (group B). We determined prothrombin time, activated partial thromboplastin time, clotting factor VIII activity, tissue plasminogen activator activity, tissue plasminogen activator inhibitor-1, cross-linked fibrin degradation product, and thrombin-antithrombin III complexes. There was a statistically significant difference between group A and B patients when the basal samples were analyzed for thrombin-antithrombin III (p = 0.046) and d-Dimer (p = 0.005). Prothrombin fragment 1 + 2 were significantly reduced, and d-Dimer was elevated when basal blood samples were compared with the second sample in patients who developed silent events (p = 0.008 and 0.055, respectively). A plasma concentration of thrombin-antithrombin III complex was also significantly decreased when sample 2 was compared with the basal blood sample (p = 0.039). Five recurrent episodes of angina and 2 nonfatal infarctions occurred, and 4 urgent revascularization procedures were performed in group A. In group B, there was only 1 nonfatal infarction (p = 0.01). The results of the present study suggest that a time-dependent thrombotic process is detectable in the blood stream as a cyclic movement. Further studies are needed to determine if some other factors, such as intensive shear stress in the vessel wall, may activate plaque instability during asymptomatic episodes.


Asunto(s)
Angina Inestable/sangre , Isquemia Miocárdica/sangre , Trombosis/sangre , Enfermedad Aguda , Anciano , Angina Inestable/diagnóstico , Biomarcadores/sangre , Angiografía Coronaria , Electrocardiografía , Femenino , Humanos , Masculino , Persona de Mediana Edad , Infarto del Miocardio/sangre , Infarto del Miocardio/diagnóstico , Isquemia Miocárdica/diagnóstico , Periodicidad , Pronóstico , Recurrencia , Trombosis/diagnóstico
15.
Clin Cardiol ; 17(8): 433-6, 1994 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-7955590

RESUMEN

The purpose of this study was to determine whether computerized ST-segment monitoring previous to percutaneous transluminal coronary angioplasty (PTCA) and after performance of this procedure may be useful to discern a pattern of recurrent angina. For this purpose, we analyzed 57 patients (11 women and 46 men, mean age 60 years) before and after treatment with balloon procedure. These patients were followed up during the next 3 months. A computerized electrocardiographic (ECG) device was used which was capable of recording simultaneously all 12 leads at rest. It was programmed to store a complete ECG every 5 min and was capable of detecting any abnormal ST alteration > than 0.5 mV at 80 ms after J junction, including R-wave amplitude. Measurements were started before (mean time 7.26 h) and after (mean time 7.96 h) the procedure. The value for ST amplitude at the J junction was used as the 0 point; then the most negative depression or the most positive elevation value was considered as the most abnormal ST alteration during monitoring. The patients were followed up for 3 months for the purpose of recognizing recurrent angina or establishing the occurrence of death. Twelve patients (30%) had recurrent angina. Discriminant function analysis revealed that ST monitoring in these patients showed significant difference after PTCA in comparison with controls, both in the frontal plane [ST depression 170 +/- 52 mV vs. 231 +/- 23 (p = 0.02)] and in precordial leads [176 +/- 16 vs. 80 +/- 6.19 mV (p = 0.0001)].(ABSTRACT TRUNCATED AT 250 WORDS)


Asunto(s)
Angina de Pecho/diagnóstico , Angioplastia Coronaria con Balón , Diagnóstico por Computador , Electrocardiografía/métodos , Adulto , Anciano , Angina de Pecho/fisiopatología , Electrocardiografía/instrumentación , Femenino , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Proyectos Piloto , Estudios Prospectivos , Recurrencia
16.
Bol Med Hosp Infant Mex ; 47(11): 783-6, 1990 Nov.
Artículo en Español | MEDLINE | ID: mdl-2285468

RESUMEN

Two cases of hemolytic-uremic syndrome (HUS) with colonic gangrene are analyzed. Colonic gangrene is an uncommonly reported complication, and it determines a greater severity of the disease, and consequently additional therapeutic measures should be taken. One of the patients died and the survivor needed colonic resection, external ileostomy, and arteriovenous hemofiltration to overcome his renal failure. In the long term follow up this patient recovered uneventfully. The initial features were similar for most of the patients with this disease. The sudden decompensation with hypotension, toxic aspect, bulged and painful abdomen, with blood stools, could indicate this complication. The patient that was undertaken to an aggressive surgical approach had a favorable outcome. In the other patient, complications such as sepsis and septic shock were present with fatal outcome. We believe that this was in part due to the delayed surgical treatment. Reports in the literature suggest a poor prognosis in the presence of this complication, because of protracted renal damage and chronic renal failure.


Asunto(s)
Colon/patología , Síndrome Hemolítico-Urémico/complicaciones , Terapia Combinada , Urgencias Médicas , Femenino , Síndrome Hemolítico-Urémico/diagnóstico , Síndrome Hemolítico-Urémico/patología , Síndrome Hemolítico-Urémico/terapia , Humanos , Ileostomía , Lactante , Necrosis/diagnóstico , Necrosis/patología , Necrosis/terapia , Reoperación
17.
Rev Chil Pediatr ; 61(3): 124-7, 1990.
Artículo en Español | MEDLINE | ID: mdl-2077581

RESUMEN

This study was conducted to evaluate usefulness of the physiologic stability index (PSI) in a pediatric intensive care Unit (PICU) of a University based hospital at Santiago, Chile. Subjects include all patients (n = 152) consecutively admitted to our PICU between april 1987 and august 1988. The PSI system was applied during the first four days from admission. The age of the patients ranged from 10 days to 15 years. Scores obtained from survivors and non survivors were compared using an analysis of variance. For maximum PSI, survivors had significantly lower scores (9.39 points) that non survivors (21.71 points). Multiple logistic regression was used to display associations between mean obtained scores and probability of death and a curve was plotted which is similar to that obtained in previous experiences from other countries.


Asunto(s)
Cuidados Críticos , Unidades de Cuidado Intensivo Pediátrico , Índice de Severidad de la Enfermedad , Adolescente , Niño , Preescolar , Estudios de Factibilidad , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Pronóstico , Análisis de Regresión , Factores de Riesgo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA