RESUMEN

OBJECTIVE: To describe a novel dissector device useful in laparoscopy, better definition of anatomic structures to have a better dissection, separation, and cleaning of the structures. METHOD: The endoscopic dissector DisePad was designed and developed at the experimental surgery department of Centro Médico Nacional 20 de Noviembre, and properly patented at Instituto Mexicano de la Propiedad Industrial (title 3512). RESULTS: The tip of the device is the most important component, by its direct contact with the different tissues, consists of a cotton-polyester black cloth impregnated with a special gel immersed into a hot saline solution. Once soaked the tip maintains the solution temperature on itself. CONCLUSIONS: This device has been used in 364 laparoscopic procedures demonstrating, its utility to visualize, separate and clean anatomical structures without thermal lesion, tear, hemorrhage or visceral perforation.

OBJETIVO: Describir un nuevo dispositivo disector en laparoscopia, con una mejor definición de las estructuras anatómicas para obtener una mejor disección,separación y limpieza de las estructuras. MÉTODO: El disector endoscópico DisePad fue diseñado y desarrollado en el servicio de cirugía experimental del Centro Médico Nacional 20 de Noviembre, y patentado ante el Instituto Mexicano de la Propiedad Industrial (registro n.º 3512). RESULTADOS: El componente más importante del disector es la punta que tiene contacto con los tejidos: es una tela de algodón-poliéster negra impregnada en un gel (patentado) que, al ser sumergido en un termo con solución salina caliente, permite retener la temperatura. CONCLUSIONES: Este dispositivo ha sido utilizado en 364 procedimientos quirúrgicos por vía laparoscópica y ha demostrado ser útil para visualizar, separar y limpiar estructuras anatómicas sin producir daño por lesión térmica, desgarre, hemorragia ni perforación visceral.

Asunto(s)

RESUMEN

Grafting is the preferred treatment for severe skin burns. Frequently, allogeneic tissue is the only transient option for wound coverage, but their use risks damage to surrounding tissues. MicroRNAs have been associated with acute rejection of different tissues/organs. In this study, we analyzed the expression of miR-31, miR-155, and miR-221 and associate it with graft tolerance or rejection using a murine full-thickness skin transplantation model. Recipient animals for the syngeneic and allogeneic groups were BALB/c and C57BL/6 mice, respectively; donor tissues were obtained from BALB/c mice. After 7 days posttransplantation (DPT), the recipient skin and grafts in the syngeneic group maintained most of their structural characteristics and transforming growth factor (TGF)-ß1 and TGF-ß3 expression. Allografts were rejected early (Banff grades II and IV at 3 and 7 DPT, respectively), showing damage to the skin architecture and alteration of TGF-ß3 distribution. miRNAs skin expression changed in both mouse strains; miR-31 expression increased in the recipient skin of syngeneic grafts relative to that of allogeneic grafts at 3 and 7 DPT (P < .05 and P < .01, respectively); miR-221 expression increased in the same grafts at 7 DPT (P < .05). The only significant difference between donor tissues was observed for miR-155 expression at 7 DPT which was associated with necrotic tissue. Only miR-31 and miR-221 levels were increased in the blood of BALB/c mice that received syngeneic grafts after 7 DPT. Our data suggest that local and systemic miR-31 and miR-221 overexpression are associated with graft tolerance.

Asunto(s)

RESUMEN

Peripheral blood mononuclear cells (PBMCs) contain a cell fraction of mononuclear progenitor cells (MPCs), which own significant angiogenic potential. Autologous transplant of PBMC and/or platelet-rich plasma (PRP) promotes endothelial cells differentiation in experimental lower limb ischemia, which is considered a safe and effective strategy to support revascularization, either in animal models or clinical trials. In addition, thrombin has been proposed to enrich biological scaffolds, hence increasing MPC viability after intramuscular administration, whereas proangiogenic mediators such as vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNF-α), inhibitor of the plasminogen activator-1 (PAI-1), and chemokine (CXCL1; GRO-α) participate in the endothelial response to ischemia, through their proangiogenic effects over endothelial cells proliferation, survival, migration, endothelial integrity maintenance, and physiologic vascular response to injury. In the present study, we describe the effect of autologous PBMCs transplant and PRP, either with or without thrombin, over proangiogenic mediators (measured by enzyme-linked immunosorbent assay) and revascularization response (angiographic vascular pattern at 30 days after vascular occlusion) in a rat model of lower limb ischemia. The group treated with PBMC + PRP significantly induced PAI-1, an effect that was prevented by the addition of thrombin. Furthermore, treatment with PBMC + PRP + thrombin resulted in the induction of VEGF. GRO-α showed a sensitive induction of all proangiogenic mediators. All treatments significantly stimulated revascularization, according to angiographic assessment, whereas higher effect was observed with PBMC + PRP treatment (p < .0001). In conclusion, autologous PBMC transplant stimulates revascularization during experimental ischemia of the lower limb, whereas particular effects over proangiogenic and fibrinolytic mediators may be attributed to PBMCs and its combination with PRP and thrombin.

Asunto(s)

RESUMEN

The synthesis of four amphiphilic organometallic complexes with the general formula RC = M(CO)5NH(CH2)15CH3, where R is a ferrocenyl 2(a-b) or a phenyl 4(a-b) group as a donor moiety and a Fischer carbene of chromium (0) or tungsten (0) as an acceptor group, are reported. These four push-pull systems formed Langmuir (L) monolayers at the air-water interface, which were characterized by isotherms of surface pressure versus molecular area and compression/expansion cycles (hysteresis curves); Brewster angle microscopic images were also obtained. By using the Langmuir-Blodgett (LB) method, molecular monolayers were transferred onto glass substrates forming Z-type multilayers. LB films were characterized through ultraviolet-visible spectroscopy, atomic force microscopy and X-ray diffraction techniques. Results indicated that films obtained from 2b complex [(Ferrocenyl)(hexadecylamine)methylidene] pentacarbonyl tungsten (0) are the most stable and homogeneous; due to their properties, these materials may be incorporated into organic electronic devices.

RESUMEN

SUMMARY The experiment evaluated the performance of beef calvos confined during 84 days and fed with one of the following diets: T1 - corn silage plus concentrate (32.65%, dry matter basis), T2 - elephant grass (Pennisetum purpureum, Schum.) napiergrass silage plus concentrate (33.36%) and T3 - napiergrass silage plus concentrate (48.27%). Daily dry matter consumption was not affected (P 0.2059) by the type of diet. Average daily gain was similar for T1 and T3 calves, and higher (P 0.0450) than observed for T2 calves. Feed conversion was not affected (P 0.1292) by the type of diet.

RESUMO Foi avaliado o desempenho em confinamento, por um período de 84 dias, de terneiros de corte alimentados com uma das seguintes dietas: T1 - silagem de milho (Zea mays, L) + concentrado (32,65%), T2 - silagem de capim elefante (Pennisetum purpureum, Schum.) cultivar napier + concentrado (33,36%) e T3 - silagem de napier + concentrado (48,27%). O tipo de dieta não influenciou (P 0,2059) no consumo médio diário de matéria seca. As dietas T3 e T1 proporcionaram ganhos de peso médio diário similares e que foram superiores (P 0,0450) ao proporcionado pela T2. Com relação a conversão alimentar, não houve diferença (P 0,1292) entre tratamentos.

RESUMEN

SUMMARY The experiment evaluated the performance of beef calvos confined during 84 days and fed with one of the following diets: T1 - corn silage plus concentrate (32.65%, dry matter basis), T2 - elephant grass (Pennisetum purpureum, Schum.) napiergrass silage plus concentrate (33.36%) and T3 - napiergrass silage plus concentrate (48.27%). Daily dry matter consumption was not affected (P 0.2059) by the type of diet. Average daily gain was similar for T1 and T3 calves, and higher (P 0.0450) than observed for T2 calves. Feed conversion was not affected (P 0.1292) by the type of diet.

RESUMO Foi avaliado o desempenho em confinamento, por um período de 84 dias, de terneiros de corte alimentados com uma das seguintes dietas: T1 - silagem de milho (Zea mays, L) + concentrado (32,65%), T2 - silagem de capim elefante (Pennisetum purpureum, Schum.) cultivar napier + concentrado (33,36%) e T3 - silagem de napier + concentrado (48,27%). O tipo de dieta não influenciou (P 0,2059) no consumo médio diário de matéria seca. As dietas T3 e T1 proporcionaram ganhos de peso médio diário similares e que foram superiores (P 0,0450) ao proporcionado pela T2. Com relação a conversão alimentar, não houve diferença (P 0,1292) entre tratamentos.