RESUMEN
We have compared the immunosuppressive effect of delta HOP and glucocorticoids on lymphocyte proliferation and IL-1 secretion. The new synthetic steroid only inhibited proliferation of phytohaemagglutinin (PHA)-stimulated human lymphocyte at high concentrations and the effect did not persist after washing out the steroid. In contrast, glucocorticoids produced the classical dose-response inhibition and the effect persisted when they were removed from the cultured medium. Although both steroids decreased IL-1 secretion from human monocytes stimulated with lipopolysaccharide (LPS), they exert the same effect through a different mechanism. The experiments we report suggest that the decrease of IL-1 synthesis produced by delta HOP could be caused by inhibition of LPS phagocytosis. These results support our hypothesis that delta HOP exerts its immunosuppressive and anti-inflammatory effect by a non-genomic mechanism.
Asunto(s)
Hidroxiprogesteronas/farmacología , Activación de Linfocitos/efectos de los fármacos , Adulto , Animales , Candida albicans/inmunología , Glucocorticoides/farmacología , Humanos , Inmunosupresores , Técnicas In Vitro , Interleucina-1/metabolismo , Macrófagos/inmunología , Fagocitosis/efectos de los fármacos , RatasRESUMEN
Insulin receptors and he hormone action "in vitro" and "in vivo" on alpha-aminoisobutyric acid (AIB) uptake were studies on thymocytes from adrenalectomized and adrenalectomized-diabetic rats. The lack of insulin effect "in vitro" correlated with the absence of insulin receptors. Meanwhile the increase in AIB uptake produced by an injection of insulin suggest an indirect action of the hormone that could be the consequences of metabolic events.
Asunto(s)
Adrenalectomía , Ácidos Aminoisobutíricos/metabolismo , Diabetes Mellitus Experimental/metabolismo , Insulina/farmacología , Timo/citología , Animales , Sitios de Unión , Femenino , Masculino , Ratas , Receptor de Insulina/análisisRESUMEN
Insulin receptors and he hormone action [quot ]in vitro[quot ] and [quot ]in vivo[quot ] on alpha-aminoisobutyric acid (AIB) uptake were studies on thymocytes from adrenalectomized and adrenalectomized-diabetic rats. The lack of insulin effect [quot ]in vitro[quot ] correlated with the absence of insulin receptors. Meanwhile the increase in AIB uptake produced by an injection of insulin suggest an indirect action of the hormone that could be the consequences of metabolic events.
RESUMEN
The injection of streptozotocin in intact or adrenalectomized rats produced a decrease in the number of thymus lymphoid cells. Bovine serum albumin gradient analyses of thymocytes from control, adrenalectomized, adrenalectomized-diabetic and diabetic animals showed variations in the mature/immature cell ratio. No remarkable changes in the distribution of the different thymocyte subpopulations were observed.
Asunto(s)
Adrenalectomía , Diabetes Mellitus Experimental/patología , Timo/patología , Animales , Fraccionamiento Celular , Masculino , Tamaño de los Órganos , Ratas , EstreptozocinaRESUMEN
The injection of streptozotocin in intact or adrenalectomized rats produced a decrease in the number of thymus lymphoid cells. Bovine serum albumin gradient analyses of thymocytes from control, adrenalectomized, adrenalectomized-diabetic and diabetic animals showed variations in the mature/immature cell ratio. No remarkable changes in the distribution of the different thymocyte subpopulations were observed.
RESUMEN
The injection of streptozotocin in intact or adrenalectomized rats produced a decrease in the number of thymus lymphoid cells. Bovine serum albumin gradient analyses of thymocytes from control, adrenalectomized, adrenalectomized-diabetic and diabetic animals showed variations in the mature/immature cell ratio. No remarkable changes in the distribution of the different thymocyte subpopulations were observed.