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Microsc Res Tech ; 64(3): 269-8, 2004 Jun 15.
Artículo en Inglés | MEDLINE | ID: mdl-15452894

RESUMEN

Chondrocytes are capable of engulfing latex particles, cell detritus, and necrotic and apoptotic remains in vitro. It is conceivable that chondrocytes might be involved in the clearance by phagocytosis of different materials within the cartilage. In fact, so far there is no evidence for the presence of "professional phagocytes" (macrophages and neutrophils) in this tissue. Chondrocyte suspensions obtained from rat knees and hips were cultured to assess phagocytosis of latex particles (1 microm), articular cartilage detritus, and necrotic and apoptotic chondrocyte remains (induced by VP-16 1 mM). We observed that chondrocytes phagocytosed latex particles as evaluated by confocal microscopy and flow cytometry. In addition, we observed that chondrocytes phagocytosed articular cartilage detritus and necrotic and apoptotic VP-16 induced-chondrocytes, as observed by bright field microscopy and transmission electron microscopy.


Asunto(s)
Cartílago Articular/fisiopatología , Condrocitos/fisiología , Fagocitosis/fisiología , Animales , Apoptosis/fisiología , Condrocitos/ultraestructura , Citometría de Flujo , Masculino , Microscopía Confocal , Microscopía Electrónica de Transmisión , Microesferas , Ratas , Ratas Wistar
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