RESUMEN
Recent evidence has demonstrated that inquiry-based physiology laboratories improve students' critical- and analytical-thinking skills. We implemented inquiry-based learning into three physiology courses: Comparative Vertebrate Physiology (majors), Human Physiology (majors), and Human Anatomy and Physiology (nonmajors). The aims of our curricular modifications were to improve the teaching of physiological concepts, teach students the scientific approach, and promote creative and critical thinking. We assessed our modifications using formative (laboratory exams, oral presentations, and laboratory reports) and summative evaluations (surveys, laboratory notebook, and an end of semester project). Students appreciated the freedom offered by the new curriculum and the opportunity to engage in the inquiry process. Results from both forms of evaluation showed a marked improvement due to the curricular revisions. Our analyses indicate an increased confidence in students' ability to formulate questions and hypotheses, design experiments, collect and analyze data, and make conclusions. Thus, we have successfully incorporated inquiry-based laboratories in both major and nonmajor courses.
Asunto(s)
Fisiología/educación , Enseñanza , Curriculum , Demografía , Humanos , Objetivos OrganizacionalesRESUMEN
In birds, the kidneys and lower intestine function in osmoregulation. A 271-amino acid homologue to aquaporin-1 (AQP-1) was isolated from the kidneys, cecae, proximal and distal rectum, and coprodeum of the lower intestine in the house sparrow (Passer domesticus). This protein has six transmembrane domains connected by two cytoplasmic loops and three extracellular loops. It exhibits 94%, 88%, and 78% homology to AQP-1 sequences of chicken, human and toad, respectively. Many of the highly conserved amino acids that are characteristic of AQP-1 are found in the sparrow sequence. RT-PCR was performed and the presence of AQP-1 mRNA was detected in the kidney and all four regions of the lower intestine. Immunoblots of total protein identified a 28-kDa non-glycosylated AQP-1 band and a 56-kDa glycosylated AQP-1 band in the kidney and all four regions of the lower intestine. Immunohistochemistry demonstrated the presence of the AQP-1 protein within both the renal cortex and medulla. In the lower intestine, the protein was present in the proximal rectum, distal rectum, and in the coprodeum. As AQP-1 functions to allow water movement across mammalian cell membranes, its presence in water-permeable cells in a bird suggests it may have a similar function.
Asunto(s)
Acuaporina 1/genética , Acuaporina 1/metabolismo , Riñón/metabolismo , Tracto Gastrointestinal Inferior/metabolismo , Homología de Secuencia de Aminoácido , Gorriones/metabolismo , Secuencia de Aminoácidos , Animales , Acuaporina 1/química , Regulación de la Expresión Génica , Immunoblotting , Inmunohistoquímica , Riñón/citología , Tracto Gastrointestinal Inferior/citología , Datos de Secuencia Molecular , Transporte de Proteínas , ARN Mensajero/genética , ARN Mensajero/metabolismo , Análisis de Secuencia de ProteínaRESUMEN
Tissue from the lower intestine of two species of sparrow, the house sparrow Passer domesticus and savannah sparrow Passerculus sandwichensis was sectioned in an unbiased manner and examined quantitatively using stereology. The tissue was processed for light microscopy, and scanning and transmission electron microscopy to examine the extent to which microvilli enhanced the epithelial surface area of the cecae, rectum, and coprodeum. Parameters measured included individual microvillus surface area, microvilli packing density, and absolute surface area. In both species, the average surface area, packing density, and absolute surface area of microvilli decreased distally along the rectum and coprodeum. All three measured variables were not statistically significant (P > 0.05) between species. Surface area amplification on the cecae due to microvilli was low, and approximated values equivalent to distal regions of the rectum and coprodeum. In the cecae, microvilli within the savannah sparrow had a significantly higher (P < 0.05) individual surface area, packing density, and absolute surface area than in the house sparrow. The functional implications of a change in microvilli population are discussed in relation to retrograde peristalsis within the lower intestine of birds.
Asunto(s)
Intestino Grueso/anatomía & histología , Peristaltismo/fisiología , Pájaros Cantores/fisiología , Agua/metabolismo , Animales , Ambiente , Secreciones Intestinales , Intestino Grueso/fisiología , Masculino , Microscopía Electrónica de Rastreo , Microvellosidades/ultraestructura , Pájaros Cantores/anatomía & histologíaRESUMEN
Seasonal variability in kidney morphology of the house sparrow Passer domesticus was examined using light microscopy. Sparrows were captured from the wild in winter, spring, summer and autumn. The kidneys were perfused with half-strength Karnovsky's fixative and processed for light microscopy by embedding in either paraffin wax or JB4 acrylic resin. Absolute volumes of the kidneys, their components (cortex, medulla and blood vessels), components of the nephron (renal corpuscles, proximal tubules, loops of Henle, distal tubules and collecting ducts) and the capillaries surrounding the nephron were quantified using stereology. Tissue processed in paraffin wax had a mean shrinkage of 17.7 % compared with 10.1 % in JB4 resin. Absolute volumes of the kidneys and nephrons were compared statistically between tissue processing methods and among seasons. Absolute volumes of the structures within the kidneys were not significantly different between treatments or among seasons. Within the nephron, the only measured variables to show significant differences were the absolute volumes of the distal tubules and cortical collecting ducts between tissue processing treatments. Thus, kidney morphology was relatively unaffected by changes in season. In addition, the results show that embedding tissue using acrylic resin causes less shrinkage, and it should therefore be the preferred embedding medium for quantitative morphologists.
Asunto(s)
Riñón/anatomía & histología , Estaciones del Año , Pájaros Cantores/anatomía & histología , Resinas Acrílicas , Animales , Túbulos Renales Colectores/anatomía & histología , Túbulos Renales Distales , Túbulos Renales Proximales/anatomía & histología , Asa de la Nefrona/anatomía & histología , Masculino , Nefronas/anatomía & histología , Pájaros Cantores/fisiología , Adhesión del TejidoRESUMEN
A mathematical model was used to investigate how concentrated urine is produced within the medullary cones of the quail kidney. Model simulations were consistent with a concentrating mechanism based on single-solute countercurrent multiplication and on NaCl cycling from ascending to descending limbs of loops of Henle. The model predicted a urine-to-plasma (U/P) osmolality ratio of approximately 2.26, a value consistent with maximum avian U/P osmolality ratios. Active NaCl transport from descending limb prebend thick segments contributed 70% of concentrating capability. NaCl entry and water extraction provided 80 and 20%, respectively, of the concentrating effect in descending limb flow. Parameter studies indicated that urine osmolality is sensitive to the rate of fluid entry into descending limbs and collecting ducts at the cone base. Parameter studies also indicated that the energetic cost of concentrating urine is sensitive to loop of Henle population as a function of medullary depth: as the fraction of loops reaching the cone tip increased above anatomic values, urine osmolality increased only marginally, and, ultimately, urine osmolality decreased.
Asunto(s)
Aves/metabolismo , Capacidad de Concentración Renal/fisiología , Algoritmos , Animales , Cloruros/orina , Coturnix , Médula Renal/anatomía & histología , Médula Renal/metabolismo , Túbulos Renales/anatomía & histología , Túbulos Renales/metabolismo , Túbulos Renales Colectores/metabolismo , Cinética , Asa de la Nefrona/anatomía & histología , Asa de la Nefrona/metabolismo , Modelos Biológicos , Modelos Teóricos , Concentración OsmolarRESUMEN
The organization of the renal medulla of the Gambel's quail, Callipepla gambelii, kidney was examined to determine the number of loops of Henle and collecting ducts and the surface area occupied by the different nephron segments as a function of distance down the medullary cones. Eleven medullary cones were dissected from the kidneys of four birds, and the tissue was processed and sectioned for light microscopy. In addition, individual nephrons were isolated on which total loop thin descending segment and thick prebend segment lengths were measured. The results show no correlation between the absolute number of loops of Henle and the length of the medullary cones. The number of thick and thin limbs of Henle and collecting ducts decrease exponentially with distance toward the apex of the cones and the rate of decrease is similar for cones of different lengths. Initially there is a rapid decrease in the number of thin limbs of Henle, indicating that most nephrons do not penetrate the cones a great distance. Thick descending limbs of Henle (prebend segment) ranged in length from 50 to 770 microm, and there was little correlation with the total length of the loop of Henle. However, the length of the thin limb of Henle correlated well with total loop length. The cell surface areas of the limbs of the loop of Henle and the collecting ducts decreased toward the apex of the cones.
Asunto(s)
Médula Renal/anatomía & histología , Médula Renal/fisiología , Codorniz/anatomía & histología , Animales , Capacidad de Concentración Renal , Túbulos Renales Colectores/anatomía & histología , Túbulos Renales Proximales/anatomía & histología , Asa de la Nefrona/anatomía & histología , Microscopía Electrónica , Nefronas/anatomía & histologíaRESUMEN
The renal anatomy of three species of sparrows, two from mesic areas, the House Sparrow (Passer domesticus) and Song Sparrow (Melospiza melodia), and one salt marsh species, the Savannah Sparrow (Passerculus sandwichensis) was examined. Electron microscopy was used to describe the ultrastructure of the nephron. In addition, stereology was used to quantify the volumes of cortex, medulla, and major vasculature of the kidneys, and the volumes and surface areas occupied by individual nephron components. There appeared to be no differences in the ultrastructural anatomy of the nephrons among the sparrows. Proximal tubules contained both narrow and wide intercellular spaces filled with interdigitations of the basolateral membrane. The thin limbs of Henle contained very wide intercellular spaces which were absent in the thick limbs of Henle. The distal tubule cells contained short, apical microvilli and infoldings of the basolateral membrane. In cross section, the medullary cones of all birds display an outer ring of thick limbs of Henle which surround an inner ring of collecting ducts, which in turn surround a central core of thin limbs of Henle. The Savannah Sparrow has a significantly higher volume of medulla compared to the two more mesic species. Within the cortex, the Savannah Sparrow also has a significantly higher volume of proximal tubules but a significantly lower volume of distal tubules than the other species. Within the medulla, the Savannah Sparrow has a significantly higher volume and surface area of capillaries, and a significantly higher surface area of thick limbs of Henle and collecting ducts than the mesic species. These data suggest that the salt marsh Savannah Sparrow has the renal morphology necessary to produce a more highly concentrated urine than the mesic zone species.
Asunto(s)
Ambiente , Riñón/anatomía & histología , Pájaros Cantores/anatomía & histología , Animales , Femenino , Riñón/ultraestructura , Masculino , Microscopía ElectrónicaRESUMEN
The anionic charge barrier and the endothelial and epithelial pore sizes on the glomerular filtration barrier (GFB) were examined in white leghorn chickens (Gallus gallus). Ruthenium red was used to stain anionic charge sites on the GFB. The tissue was treated by normal dehydration and freeze substitution dehydration for transmission electron microscopy (TEM). In addition, the basal lamina was isolated for study. The results of our study indicate that G. gallus possess a thick, negatively charged glycocalyx surrounding the podocytes and slit diaphragm and on the endothelium. However, in all cases, little anionic charge is present in the basal lamina. The pores on the endothelium are elliptical and have mean dimensions of 148 x 110 nm. This is in contrast to mammals, which have smaller, round pores. The epithelial pores in G. gallus measure approximately 35 nm in length, approximately 4 times larger than those found in mammals. These results indicate that the avian glomerulus may allow the filtration of larger molecules from the plasma than occurs in mammals and that the charge on the molecule may not be as restrictive a filtration characteristic as in mammals.
Asunto(s)
Pollos/fisiología , Endotelio Vascular/ultraestructura , Glomérulos Renales/ultraestructura , Animales , Aniones/metabolismo , Endotelio Vascular/metabolismo , Femenino , Glomérulos Renales/citología , Glomérulos Renales/fisiología , Túbulos Renales/metabolismo , Microscopía Electrónica , Microscopía Electrónica de Rastreo , Porinas/metabolismo , Porinas/ultraestructura , Ácido Úrico/metabolismoRESUMEN
The glomerular capillary architecture of nephrons that include a loop of Henle (looped) and those that lack the loop (loopless) nephrons was examined qualitatively and quantitatively by electron microscopy in Gallus gallus and Callipepla gambelii. The glomerular capillaries of looped nephrons form a dichotomously branched network, while those of loopless nephrons are arranged loosely, and the majority are unbranched. There was no significant difference in the diameter of the glomerular capillaries between looped and loopless nephrons; however, in all cases the diameter of the afferent arteriole was significantly larger than that of the efferent arteriole. Based on size alone, the predicted blood flow rate in the efferent arteriole is 20% that of the afferent arteriole in G.gallus and 7% that of the afferent arteriole in C.gambelii. There was no significant difference in the volume density (Vv) of the glomerular capillaries between looped and loopless nephrons. However, the surface area density (Sv) of the glomerular capillaries in loopless nephrons of C.gambelii was significantly larger than for the looped nephrons, and for the loopless nephrons in G. gallus. This suggests that there may be a decrease in blood flow rate along the glomerular capillaries of the loopless nephrons in C. gambelii. Overall, the results indicate that the avian glomerular capillaries are less complex than those of mammals.
Asunto(s)
Pollos/anatomía & histología , Glomérulos Renales/irrigación sanguínea , Codorniz/anatomía & histología , Animales , Capilares/anatomía & histología , Capilares/ultraestructura , Microscopía Electrónica , Microscopía Electrónica de RastreoRESUMEN
The qualitative ultrastructural renal anatomy was examined in 4 species of honeyeater (parvorder Corvi) inhabiting 2 distinctly different environments. The kidneys of the wet zone New Holland honeyeater Phylidonyris novaehollandiae and little wattlebird Anthochaera lunulata were compared with those of the arid zone white-fronted honeyeater Phylidonyris albifrons and spiny-cheeked honeyeater Acanthogenys rufogularis. The size and structure of glomeruli were similar between species. In all species, except in P. novaehollandiae, the proximal tubule cells contained wide intercellular spaces filled with basolateral cell membrane interdigitations. Medullary nephron tubules were arranged in a sequential manner in all species. Thick and thin limbs of Henle were separated by the collecting ducts and extended the entire length of the medulla, a situation not found in muscicapid passerines. This tubular arrangement is not entirely consistent with the proposed single-effect countercurrent multiplier theory. The thin limb of Henle consisted of only one epithelium type, which had wide intercellular spaces. The thick limb of Henle consisted of 2 types of epithelial cells, each having narrow intercellular spaces, but with varying degrees of cell membrane infoldings. The ultrastructural morphology of the limbs of Henle in honeyeaters differed from those of muscicapid passerines. The ultrastructure of the distal nephron was similar in each species studied. All of the above nephron characteristics are considered to enable honeyeaters to absorb a large proportion of solutes and water from the glomerular filtrate.
Asunto(s)
Aves/anatomía & histología , Clima , Riñón/ultraestructura , Animales , Glomérulos Renales/ultraestructura , Túbulos Renales/ultraestructura , Microscopía Electrónica , Microscopía Electrónica de Rastreo , Nefronas/ultraestructuraRESUMEN
Stereology was used to quantify components within the kidney of honeyeater birds. Arid zone and wet zone inhabiting 'matched' body mass pairs of birds were examined. The kidney structure of the arid zone white-fronted honeyeater, Phylidonyris albifrons (16.9 g), was compared with that of the wet zone New Holland honeyeater, Phylidonyris novaehollandiae (21.9 g), and that of the arid zone spiny-cheeked honeyeater, Acanthogenys rufogularis (42.5 g), with that of the wet zone little wattlebird, Anthochaera lunulata (62.0 g). Both arid zone honeyeaters had a significantly higher (P less than 0.001) percentage of medulla in the kidneys, while the wet zone birds had a significantly higher (P less than 0.001) percentage of cortex. There were few differences between arid and wet zone honeyeaters in the percentage of nephron components in the cortex and medulla. Both arid zone bird species had a significantly larger volume of medulla, a feature characteristic of a high ability to conserve water by producing a concentrated urine. Both wet zone species had a higher volume of cortex but the difference was not significant. Few differences were found in the volumes and surface areas of tubules within the nephron. Differences that did occur were not always consistent with a high ability to conserve either ions or water more efficiently. The volume and surface area of brush border in the proximal tubule were significantly higher in the little wattlebird. This characteristic may lead to a greater capacity of its kidneys to absorb both water and ions.
Asunto(s)
Aves/anatomía & histología , Riñón/anatomía & histología , Animales , Clima , Riñón/irrigación sanguínea , Corteza Renal/anatomía & histología , Médula Renal/anatomía & histología , Australia OccidentalRESUMEN
Despite their close taxonomic affinities, nectar-feeding passerine birds from Australia had smaller kidneys, on average, than sympatric passerines of equivalent weight that fed entirely upon insects. Insectivorous passerines from North America had larger kidneys, on average, than comparable insect-feeding passerines from the separate endemic radiation in Australia. Dietary and other environmental differences, rather than phylogenetic origins, may account for these differences. The left kidney of Australian passerines was significantly longer, on average, than the right. Kidney widths showed no lateral asymmetry.