RESUMEN

Telomerase maintains telomere length at the ends of linear chromosomes using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). An essential part of TER is the template/pseudoknot domain (t/PK) which includes the template, for adding telomeric repeats, template boundary element (TBE), and pseudoknot, enclosed in a circle by stem 1. The Tetrahymena telomerase holoenzyme catalytic core (p65-TER-TERT) was recently modeled in our 9 Šresolution cryo-electron microscopy map by fitting protein and TER domains, including a solution NMR structure of the Tetrahymena pseudoknot. Here, we describe in detail the structure and folding of the isolated pseudoknot, which forms a compact structure with major groove U•A-U and novel C•G-A+ base triples. Base substitutions that disrupt the base triples reduce telomerase activity in vitro NMR studies also reveal that the pseudoknot does not form in the context of full-length TER in the absence of TERT, due to formation of a competing structure that sequesters pseudoknot residues. The residues around the TBE remain unpaired, potentially providing access by TERT to this high affinity binding site during an early step in TERT-TER assembly. A model for the assembly pathway of the catalytic core is proposed.

Asunto(s)

Pliegue del ARN , ARN Protozoario/química , ARN/química , Telomerasa/química , Tetrahymena thermophila/química , Secuencia de Bases , Dominio Catalítico , Humanos , ARN/metabolismo , ARN Protozoario/metabolismo , Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/metabolismo , Alineación de Secuencia , Telomerasa/metabolismo , Homeostasis del Telómero , Tetrahymena thermophila/metabolismo

RESUMEN

Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). We report the cryo-electron microscopy structure of Tetrahymena telomerase at ~9 angstrom resolution. In addition to seven known holoenzyme proteins, we identify two additional proteins that form a complex (TEB) with single-stranded telomere DNA-binding protein Teb1, paralogous to heterotrimeric replication protein A (RPA). The p75-p45-p19 subcomplex is identified as another RPA-related complex, CST (CTC1-STN1-TEN1). This study reveals the paths of TER in the TERT-TER-p65 catalytic core and single-stranded DNA exit; extensive subunit interactions of the TERT essential N-terminal domain, p50, and TEB; and other subunit identities and structures, including p19 and p45C crystal structures. Our findings provide structural and mechanistic insights into telomerase holoenzyme function.

Asunto(s)

ARN/química , Telomerasa/química , Tetrahymena/enzimología , Dominio Catalítico , Microscopía por Crioelectrón , Cristalografía por Rayos X , ADN de Cadena Simple/química , Holoenzimas/química , Unión Proteica , Conformación Proteica , Subunidades de Proteína/química , Proteína de Replicación A/química , Telómero/química , Homeostasis del Telómero , Proteínas de Unión a Telómeros

RESUMEN

Telomerase is the ribonucleoprotein (RNP) reverse transcriptase responsible for synthesizing the 3' ends of linear chromosomes. It plays critical roles in tumorigenesis, cellular aging, and stem cell renewal. The past two years have seen exciting progress in determining telomerase holoenzyme architecture and the structural basis of telomerase activity. Notably, the first electron microscopy structures of telomerase were reported, of the Tetrahymena thermophila telomerase holoenzyme and a human telomerase dimer. In addition to new structures of TERT and TER domains, the first structures of telomerase protein domains beyond TERT, and their complexes with TER or telomeric single-stranded DNA, were reported. Together these studies provide the first glimpse into the organization of the proteins and RNA in the telomerase RNP.

Asunto(s)

Telomerasa/química , Animales , Humanos , Modelos Moleculares , Multimerización de Proteína , Telomerasa/metabolismo , Tetrahymena thermophila/química , Tetrahymena thermophila/enzimología

RESUMEN

Telomerase is a ribonucleoprotein complex that extends the 3' ends of linear chromosomes. The specialized telomerase reverse transcriptase requires a multidomain RNA (telomerase RNA, TER), which includes an integral RNA template and functionally important template-adjacent pseudoknot. The structure of the human TER pseudoknot revealed that the loops interact with the stems to form a triple helix shown to be important for activity in vitro. A similar triple helix has been predicted to form in diverse fungi TER pseudoknots. The solution NMR structure of the Kluyveromyces lactis pseudoknot, presented here, reveals that it contains a long pyrimidine motif triple helix with unexpected features that include three individual bulge nucleotides and a C(+)•G-C triple adjacent to a stem 2-loop 2 junction. Despite significant differences in sequence and base triples, the 3D shape of the human and K. lactis TER pseudoknots are remarkably similar. Analysis of the effects of nucleotide substitutions on cell growth and telomere lengths provides evidence that this conserved structure forms in endogenously assembled telomerase and is essential for telomerase function in vivo.

Asunto(s)

Kluyveromyces/enzimología , Conformación de Ácido Nucleico , ARN Bacteriano/química , ARN/química , Telomerasa/química , Secuencia de Bases , Humanos , Kluyveromyces/genética , Modelos Moleculares , Mutagénesis , Resonancia Magnética Nuclear Biomolecular , Pirimidinas/química , ARN/genética , Estabilidad del ARN , ARN Bacteriano/genética , ARN de Hongos/química , ARN de Hongos/genética , Saccharomyces cerevisiae/enzimología , Saccharomyces cerevisiae/genética , Telomerasa/genética

RESUMEN

Telomerase adds telomeric repeats to chromosome ends using an internal RNA template and a specialized telomerase reverse transcriptase (TERT), thereby maintaining genome integrity. Little is known about the physical relationships among protein and RNA subunits within a biologically functional holoenzyme. Here we describe the architecture of Tetrahymena thermophila telomerase holoenzyme determined by electron microscopy. Six of the seven proteins and the TERT-binding regions of telomerase RNA (TER) have been localized by affinity labelling. Fitting with high-resolution structures reveals the organization of TERT, TER and p65 in the ribonucleoprotein (RNP) catalytic core. p50 has an unanticipated role as a hub between the RNP catalytic core, p75-p19-p45 subcomplex, and the DNA-binding Teb1. A complete in vitro holoenzyme reconstitution assigns function to these interactions in processive telomeric repeat synthesis. These studies provide the first view of the extensive network of subunit associations necessary for telomerase holoenzyme assembly and physiological function.

Asunto(s)

Telomerasa/química , Telomerasa/ultraestructura , Tetrahymena thermophila/enzimología , Dominio Catalítico , Holoenzimas/química , Holoenzimas/genética , Holoenzimas/ultraestructura , Microscopía Electrónica , Modelos Moleculares , Conformación de Ácido Nucleico , Docilidad , Estructura Terciaria de Proteína , Subunidades de Proteína/análisis , Subunidades de Proteína/química , Subunidades de Proteína/metabolismo , Proteínas Protozoarias/química , Proteínas Protozoarias/metabolismo , Proteínas Protozoarias/ultraestructura , ARN/química , ARN/metabolismo , ARN/ultraestructura , Ribonucleoproteínas/química , Ribonucleoproteínas/genética , Ribonucleoproteínas/metabolismo , Ribonucleoproteínas/ultraestructura , Telomerasa/genética , Telomerasa/metabolismo , Tetrahymena thermophila/química , Tetrahymena thermophila/genética , Tetrahymena thermophila/ultraestructura