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Zatrephina lineata (Coleoptera: Chrysomelidae) is a phytophagous insect, mainly of plants of the genera Ipomoea and Mikania. The objective was to study the development, survival and to describe the life stages of Z. lineata fed on leaves of Ipomoea pes-caprae. Biological observations were made daily with the aid of a stereoscopic microscope and the instars of this insect identified by the exuvia left between one moulting and the next. The duration of development and survival of the egg, larva and pupa stages and the first, second, third, fourth and fifth instars and of the nymph stage of Z. lineata differed, but not between sexes of this insect. The duration of development of Z. lineata was longer in the larval stage and in the fifth instar, and its survival greater in the egg and pupa stages and in the first and fifth instars. Zatrephina lineata eggs, cream-colored, are ellipsoid and deposited in groups on the adaxial surface of older I. pes-caprae leaves. The larvae of this insect go through five instars, with the first three being gregarious with chemo-behavioral defenses. The exarated pupae of Z. lineata, light yellow in color and with an oval shape flattened dorsoventrally, attach to the abaxial surface of the I. pes-caprae leaves. The shape of adults of this insect is oval, straw yellow in color with lighter longitudinal stripes and females are slightly larger than males.

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OBJECTIVES: This study aimed to evaluate the adhesive properties in dentine after the application of silver diamine fluoride (SDF) on carious dentine lesions immediately and after 2 years of water storage. METHODS: 96 human molars used were subjected to artificial dentine caries production, and then randomly divided into 12 experimental groups according to 1. application of an SDF solution (carious dentine lesion without SDF treatment [control], with 12 % silver diamine fluoride [SDF 12 %] or 38 % silver diamine fluoride [SDF 38 %]); 2. Universal adhesives (Clearfil Universal Bond Quick [CUQ] and Single Bond Universal [SBU]); 3. adhesive strategy (etch-and-rinse [ER] and self-etch [SE]). After restoration, the specimens were sectioned and submitted to the microtensile bond strength test (µTBS) and energy-dispersive X-ray spectrometry analysis (SEM/EDX). All tests were performed immediately and after 2 years of water storage. Data from the µTBS were analyzed using four-way ANOVA and Tukey's test (α = 0.05). RESULTS: Only the interaction of factors 'SDF' vs 'time' was significant (p = 0.03). After 2 years of storage, the groups where SDF was applied showed higher µTBS values compared to the control group. No significant decrease in µTBS values was observed for SBU when comparing immediate and 2-year results, but a significant reduction in µTBS values was observed after 2 years for CUQ. CONCLUSION: Independent of the adhesive strategy, the use of SDF may be a promising alternative to maintain the bonding of universal adhesives to carious dentinal lesions. CLINICAL SIGNIFICANCE: This study may clarify and support clinicians regarding the longevity of resin-based restoration in caries-affected dentine treated with silver diamine fluoride.

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Lead is a heavy metal and an important cause of acute or chronic toxicosis in humans, domestic, and wild animals. This report aims to describe a case of chronic lead poisoning in a jaguar (Panthera onca) kept under human care that was rescued from the wild environment. The animal was rescued in poor condition in 2004 and kept under human care at the Belo Horizonte Zoological Garden (Minas Gerais, Brazil) until 2020, when it presented with anorexia, vomiting and ataxia. Over the past years the animal had episodes of anemia and increased serum urea and creatinine. Radiography demonstrated 21 radiopaque projectiles on the right side of the face. At necropsy there were multiple projectiles surrounded by fibrous tissue in the subcutaneous of the right side of the face, fibrinous peritonitis, multiple gastric ulcers, and melena. The lead dosage was performed using the atomic absorption spectrometry technique using renal tissue collected at necropsy, with a result of 908 ppb (µg/kg). The findings of projectiles associated with the dosage of lead above the reference limits allow the diagnosis of chronic intoxication in this case.

Chumbo é um metal pesado e uma causa importante de toxicose crônica no homem e em animais domésticos e selvagens. Este relato teve como objetivo descrever um caso de intoxicação crônica por chumbo em uma onça-pintada (Panthera onca), mantida sob cuidados humanos, que foi resgatada de seu ambiente natural em 2004, em pobre condição corporal. O animal foi encaminhado ao zoológico de Belo Horizonte (MG, Brasil), onde permaneceu até 2020, quando desenvolveu anorexia, vômitos e ataxia. Durante os últimos anos, o animal teve episódios de anemia e aumento da concentração sérica de ureia e creatinina. O exame radiográfico demonstrou 21 projéteis radiodensos na face direita. À necropsia, foram observados vários projéteis envoltos por tecido conjuntivo no subcutâneo da face esquerda, peritonite fibrinosa, múltiplas úlceras gástricas e melena. A concentração de chumbo foi determinada por espectrometria de absorção atômica em amostras de tecido renal, indicando 908 ppb (µg/kg). O achado de projéteis de chumbo associado à elevada concentração de chumbo é compatível com intoxicação crônica por chumbo neste caso.

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OBJECTIVE: To investigate the effect of fluoride-containing whitening products on sound enamel and on artificial caries lesions during a cariogenic challenge. MATERIALS AND METHODS: Bovine enamel specimens (n = 120) with three areas [non-treated sound enamel (NSE), treated sound enamel (TSE), and treated artificial caries lesion (TACL)] were randomly assigned to the four groups: whitening mouthrinse (WM: 2.5% hydrogen peroxide-100 ppm F-), placebo mouthrinse (PM: 0% hydrogen peroxide-100 ppm F-), whitening gel (WG: 10% carbamide peroxide-1130 ppm F-), and deionized water (negative control; NC). The treatments (2 min for WM, PM, and NC, and 2 h for WG) were carried out during a 28-day pH-cycling model (6 × 60 min demineralization/day). Relative surface reflection intensity (rSRI) and transversal microradiography (TMR) analyses were performed. Fluoride uptake (surface and subsurface) was measured in additional enamel specimens. RESULTS: For TSE, a higher value of rSRI was observed in WM (89.99% ± 6.94), and a greater decrease in rSRI was observed for WG and NC, and no sign of mineral loss was verified for all groups (p > 0.05). For TACL, rSRI significantly decreased after pH-cycling for all experimental groups with no difference between them (p < 0.05). Higher amounts of fluoride were found in WG. WG and WM exhibited intermediate values of mineral loss, similar to PM. CONCLUSIONS: The whitening products did not potentialize the enamel demineralization under a severe cariogenic challenge, and they did not exacerbate mineral loss of the artificial caries lesions. CLINICAL RELEVANCE: Low concentrated hydrogen peroxide whitening gel and mouthrinse containing fluoride do not intensify the progression of caries lesions.

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P-glycoprotein (P-gp, encoded by the ABCB1 gene) and breast cancer resistance protein (BCRP/ABCG2) are efflux multidrug resistance (MDR) transporters localized at the syncytiotrophoblast barrier of the placenta and protect the conceptus from drug and toxin exposure throughout pregnancy. Infection is an important modulator of MDR expression and function. This review comprehensively examines the effect of infection on the MDR transporters, P-gp and BCRP in the placenta. Infection PAMPs such as bacterial lipopolysaccharide (LPS) and viral polyinosinic-polycytidylic acid (poly I:C) and single-stranded (ss)RNA, as well as infection with Zika virus (ZIKV), Plasmodium berghei ANKA (modeling malaria in pregnancy - MiP) and polymicrobial infection of intrauterine tissues (chorioamnionitis) all modulate placental P-gp and BCRP at the levels of mRNA, protein and or function; with specific responses varying according to gestational age, trophoblast type and species (human vs. mice). Furthermore, we describe the expression and localization profile of Toll-like receptor (TLR) proteins of the innate immune system at the maternal-fetal interface, aiming to better understand how infective agents modulate placental MDR. We also highlight important gaps in the field and propose future research directions. We conclude that alterations in placental MDR expression and function induced by infective agents may not only alter the intrauterine biodistribution of important MDR substrates such as drugs, toxins, hormones, cytokines, chemokines and waste metabolites, but also impact normal placentation and adversely affect pregnancy outcome and maternal/neonatal health.

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This study describes the occurrence of reproductive changes in a mullet (Mugil curema) from the Babibtonga Bay, Santa Catarina. Gross and microscopic findings indicated changes in the reproductive system that were characterized by the presence of both male and female gonads, nearing sexual maturity, which was compatible with the diagnosis of intersex. It was not possible to identify predisposing factors that may have contributed to the development of this condition. However, as this species of fish may have their reproductive development affected by anthropogenic influence, it is important to permanently monitor this kind of environment. Considering the number of fish belonging to this species that was examined (n=433), the estimated prevalence for intersexuality was 0.23% (confidence interval: 0.00 to 0.68%, with confidence level of 95%). The findings in this study support the diagnosis of intersexuality in M. curema from the Babitonga Bay, which is the first documented case of this condition in this species on the coast of Santa Catarina, Brazil.

Este estudo descreve a ocorrência de alteração reprodutiva em peixe da espécie Mugil curema, procedente da Baía da Babitonga, Santa Catarina. Macro e microscopicamente, foi identificada alteração no sistema reprodutivo, caracterizada pela presença de gônadas masculina e feminina, próximas da maturidade sexual, caracterizando um caso de intersexo. Não foi possível determinar os fatores que possam ter contribuído para o desenvolvimento dessa alteração reprodutiva, porém, como a espécie animal pode ter suas características de vida e reprodutiva influenciadas pela ação antrópica, é importante um estudo contínuo desses peixes nesse ambiente. Considerando o número de peixes dessa espécie que foram examinados (n=433), a prevalência estimada de intersexualidade foi de 0,23% (intervalo de confiança: 0,00 a 0,68%, com nível de confiança de 95%). Os achados deste estudo suportam o diagnóstico de intersexualidade em M. curema proveniente da Baía da Babitonga, sendo o primeiro caso documentado dessa condição nessa espécie na costa de Santa Catarina, Brasil.

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Limited information is available about the effect of mid-pregnancy viral infections on the placental expression of efflux transporters and offspring behavior. We hypothesized that maternal exposure to polyinosinic-polycytidylic acid [poly(I:C)], a synthetic double-stranded RNA viral mimic, would impair placental cell turnover, the expression of selected ABC transporters and adult offspring behavior. C57BL/6 mice were administered poly(I:C) (10 mg/Kg;ip) or vehicle at gestational day (GD) 13.5 (mid-pregnancy). Dams were euthanized for blood collection 4 h after injection, fetal and placental collection at GD18.5 or allowed to deliver spontaneously at term. At GD 13.5, poly(I:C) induced an acute pro-inflammatory response characterized by an increase in maternal plasma levels of IL-6, CXCL-1 and CCL-2/MCP-1. At GD 18.5, poly(I:C) decreased cell proliferation/death in the labyrinthine and increased cell death in the junctional zones, characterizing a disruption of placental cell turnover. Abca1 and Abcg1 immunolabelling was decreased in the labyrinthine zone, whereas Abca1, Abcg1 and breast cancer resistance transporter (Bcrp) expression increased in the junctional zone. Moreover, adult offspring showed motor and cognitive impairments in the Rotarod and T-water maze tests. These results indicate that viral infection during mid-pregnancy may disrupt relevant placental efflux transporters, as well as placental cell turnover and offspring behavior in adult life.

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The concern regarding the harm caused by biocides to human health has been increasing over the years, making the natural products an alternative to less toxic and more efficient biocides. Therefore, this paper reports the investigation of the disinfectant potential of extracts and isolated compounds from Baccharis dracunculifolia. For this purpose, extracts of aerial parts (BD-C), tricomial wash (BD-L) and roots (BD-R) of B. dracunculifolia were obtained by maceration. The extracts were submitted to different chromatographic techniques, including high-speedy countercurrent chromatography (HSCCC) furnishing nine isolated compounds. The extracts and isolated compounds were evaluated regarding their antimicrobial activity by the broth microdilution method, according to the Clinical and Laboratory Standards Institute, and regarding their sanitizing activity according to Standard Operating Procedure No. 65·3210·007 (INCQS, 2011), developed by the National Institute for Quality Control in Health (INCQS) - Oswaldo Cruz Foundation (FIOCRUZ). In the antimicrobial evaluation the BD-C extract showed minimum inhibitory concentration (MIC) values of 200 and 100 µg/ml against Staphylococcus aureus and Tricophyton mentagrophytes, respectively. BD-L extract showed MIC value of 200 µg/ml against S. aureus. The isolated compounds caffeic acid (MBC 2·22 µmol l-1 ), ferulic acid (MBC 2·06 µmol l-1 ) and baccharin (MBC 0·27 µmol l-1 ) showed significant inhibitory activity against S. aureus. All B. dracunculifolia isolated compounds were active with exception of aromadrendin-4´-O-methyl-ether for T. mentagrophytes. Additionally, isosakuranetin was active against Salmonella choleraesuis (MIC 1·4 µmol l-1 ). Regarding the sanitizing activity, the hydroalcoholic solution containing 0·2% of B. dracunculifolia extract in 40°GL ethanol was effective in eliminating the microbial contamination on all carrier cylinders and against all microorganisms evaluated in the recommended exposure time of 10 min. Therefore, B. dracunculifolia has potential for the development of sanitizing agents to be used in hospitals, food manufactures and homes.

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O experimento foi realizado no Município de Penápolis/SP, no Campo Experimental da Fundação Educacional de Penápolis - FUNEPE, com o objetivo de avaliar a composição bromatológica da silagem de capim elefante (Pennisetum purpureum Schum) cv. BRS Capiaçu, utilizando aditivo ou inoculantes no processo de ensilagem. Utilizou-se o delineamento inteiramente casualizado com cinco tratamentos (T) e três repetições: T1 - capim-elefante in natura; T2 - ensilado sem o uso de aditivo ou inoculante; T3 - ensilado com 7% de farelo de milho; T4 - ensilado com 10% de polpa cítrica e T5 ­ ensilado com inoculante bacteriano 20mL/kg¹ de matéria seca (MS). As amostras ensiladas foram acondicionadas em sacos plásticos com densidade de 20 kg de matéria natural (MN) por embalagem e as análises foram feitas a partir do material obtido de plantas com 110 dias de idade. Para os materiais ensilados obedeceu-se ao tempo de 30 dias para realização das análises bromatológicas onde foram avaliados os seguintes parâmetros: matéria seca (MS), nutrientes digestíveis totais (NDT) e Proteína Bruta (PB). O capim BRS Capiaçu ensilado com 7% na matéria natural de farelo de milho apresenta maiores teores de matéria seca e proteína bruta.

The experiment was carried out in the city of Penápolis/SP, in the Experimental Field of the Educational Foundation of Penápolis - FUNEPE, with the objective of evaluating the bromatological composition of elephant grass silage (Pennisetum purpureum Schum) cv. BRS Capiaçu, using additive or inoculants in the silage process. A completely randomized design with five treatments (T) and three replications was used: T1 - elephant grass without fresh additive; T2 - ensiled without the use of additives; T3 - ensiled with 7% com bran; T4 - ensiled with 10% citrus pulp and T5 - ensiled with bacterial inoculant 20 mL/kg¹ of dry matter (DM). The ensiled samples were packed in plastic bags with a density of 20 kg of natural material (MN) per package and the analyzes were made from material obtained from 110-day-old plants. For the ensiled materials, the time of 30 days for the bromatological analysis was observed, where the following parameters were evaluated: dry matter (DM), total digestible nutrients (NDT) and Crude Protein (PB). The BRS Capiaçu grass ensiled with 7% in the natural matter of corn bran has higher contents of dry matter and protein.

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O objetivo deste trabalho foi avaliar a influência das diferentes idades de corte sobre o teor de matéria seca e proteína bruta do capim BRS Capiaçu. O experimento iniciou-se com a seleção e corte das plantas matrizes para o maior aproveitamento do colmo, fez necessário eliminar as folhas superiores, pois as mesmas não brotam, e a retirada facilitou na distribuição do colmo na hora do plantio. O delineamento experimental foi realizado em blocos casualizados, totalizando quatro repetições. A unidade experimental constituiu-se de três linhas de plantio de 48,0 m de comprimento com espaçamento entre linhas de 1,5 m, totalizando 144 m². O experimento teve duração de 110 dias, sendo distribuído em três períodos: 60, 90 e 110 dias, somando assim, três idades de corte. Para a implantação da cultivar, foi realizado o preparo convencional do solo, uso do adubo para plantio na formulação NPK 4-30-10, na cobertura o fertilizante 20-05-20 conforme a análise química do solo e o plantio por colmos no sulco. Nas três idades de corte, as amostras coletadas foram acondicionadas em sacos plásticos identificados conforme o tratamento e repetição, posteriormente foram encaminhados para o laboratório. Os maiores valores de matéria seca foram obtidos com 90 e 110 dias de corte (p<0,05). Na variável proteína bruta, o maior valor foi observado aos 60 dias, porém, não diferindo do corte realizado aos 110 dias de idade (p>0.05). O corte realizado aos 110 dias favoreceu o aumento nos teores de matéria seca sem comprometer os teores de proteína bruta.

The objective of this work was to evaluate the influence of different cutting ages on the dry matter and crude protein content of BRS Capiaçu grass. The experiment began with the selection and cutting of the mother plants for the best use of the stem, it was necessary to eliminate the upper leaves, as they do not sprout, and the removal facilitated the distribution of the stem at the time of planting. The experimental design was carried out in randomized blocks, totaling four replications. The experimental unit consisted of three planting lines of 48.0 m in length with a spacing between rows of 1.5 m, totaling 144 m². The experiment lasted 110 days, being distributed in three periods: 60, 90 and 110 days, thus adding three cutting ages. For the implantation of the cultivar, the conventional soil preparation was carried out, the use of fertilizer for planting in the NPK 4-30-10 formulation, in the coverage the fertilizer 20-05-20 according to the chemical analysis of the soil and the planting by stalks in the furrow. At the three cutting ages, the samples collected were placed in plastic bags identified according to the treatment and repetition, and were later sent to the laboratory. The highest values of dry matter were obtained with 90 and 110 days of cutting (p<0.05). In the crude protein variable, the highest value was observed at 60 days of age, however, it did not differ from the cut performed at 110 days of age (p>0.05). The cut performed at 110 days favored an increase in dry matter contents without compromising crude protein contents.

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Pulmonary adenocarcinoma is a malignant epithelial neoplasia that usually arises from conducting airways or alveolar parenchyma. It has rarely been described in wild felids, with no previous reports in ocelots. In domestic cats it is a very aggressive neoplasm with a high metastatic rate that usually evolves to death. This report aimed to describe a pulmonary adenocarcinoma in a captive and senile ocelot (Leopardus pardalis), with a thorough morphologic and immunophenotypically characterization, evidencing the epithelial-mesenchymal transition (EMT) phenomenon in a high metastatic carcinoma, an important feature rarely described in veterinary medicine, even in domestic cats.(AU)

O adenocarcinoma pulmonar é uma neoplasia epitelial maligna originada do epitélio respiratório das vias aéreas inferiores e do parênquima alveolar. É uma neoplasia raramente descrita em felinos selvagens, sem nenhum relato em jaguatiricas. Em gatos domésticos, é uma neoplasia muito agressiva, com alta taxa de metástase, e geralmente evolui para o óbito do paciente. O presente relato objetiva descrever um adenocarcinoma pulmonar em uma jaguatirica (Leopardus pardalis) senil de cativeiro, com detalhada caracterização morfológica e imunofenotípica, evidenciando o fenômeno de transição epitelial-mesenquial (TEM) em um carcinoma altamente metastático, uma característica importante, com escassos relatos na medicina veterinária, mesmo em gatos domésticos.(AU)

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A senile male captive bush dog (Speothos venaticus) presented a small perianal cutaneous nodule. Histologically, there was an ulcerated round cell tumor composed of well differentiated mast cells with abundant intracytoplasmic purple Giemsa-positive granules, with a diffuse eosinophilic infiltrate. Immunohistochemistry revealed that 30% of the neoplastic cells were positive for Kit in the cytoplasm and cell membrane, and all neoplastic cells were negative for MAC and CD3. Less than 10% of the neoplastic cells were positive for Ki67. At necropsy other primary tumors were identified in this animal, including an intestinal adenoma, an adrenal cortex adenoma and a testicular interstitial cell tumor.(AU)

Um cachorro-vinagre (Speothos venaticus) apresentou um nódulo cutâneo pequeno na região perianal. Histologicamente havia neoplasia cutânea de células redondas e ulcerada, constituída por mastócitos bem diferenciados, com abundantes grânulos citoplasmáticos metacromáticos na coloração de Giemsa e infiltrado eosinofílico difuso. A imuno-histoquímica demonstrou que 30% das células neoplásicas eram positivas para a proteína Kit no citoplasma e na membrana celular. As células foram negativas para MAC e CD3. Menos de 10% das células neoplásicas foram positivas para Ki67. Durante a necropsia, foram identificados outros tumores primários, como adenoma intestinal, adenoma cortical da adrenal e tumor de células intersticiais do testículo.(AU)

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The sugarcane cystatin (CaneCPI-5) was recently cloned and showed strong binding force to dental enamel and protection against initial erosion. However, evaluations on its safety and efficacy in a situation closer to the clinical condition are necessary. In the present study we analyzed 1) the cytotoxicity of CaneCPI-5 on human gingival fibroblasts (HGFs); 2) the ability of CaneCPI-5 to reduce enamel erosion and erosion+abrasion in situ. In part 1, HGFs were treated with CaneCPI-5 (0.025, 0.05, 0.1, 0.5 or 1.0 mg/mL) or no treatment (control). The cytotoxicity was assessed after 60 s and 24 h by mitochondrial activity (MTT), confocal microscopy, and hematoxylin/eosin staining. In part 2, 15 volunteers participated in a double-blind crossover protocol consisting of 3 phases, according to the following treatments: 1) 0.1 mg/mL CaneCPI-5; 2) SnCl2/NaF/AmF (Elmex; positive control); 3) water (negative control). The volunteers wore an appliance containing 4 bovine enamel specimens for 5 d. Each day, the specimens were individually treated with 50 µL of the tested solutions per 60 s and then subjected to erosive challenges (0.1% citric acid, pH 2.5, for 90 s, 4 times per day). After the first and last erosive challenge each day, 2 samples were abraded (toothbrushing, 15 s). Enamel wear was measured by contact profilometry. One or two-way analysis of variance (ANOVA)/Tukey's or Sidak's tests (P < 0.05) were applied. Regardless of the concentration and the experimental time, CaneCPI-5 did not decrease the cell viability compared to the negative control (P < 0.05). Erosion+abrasion led to significantly greater wear compared to erosion only. For both conditions, the lowest wear was found for SnCl2 and CaneCPI-5, which did not differ significantly from each other, but showed significant protection when compared to the negative control. In conclusion, CaneCPI-5 is safe on HGFs and reduces enamel erosive wear to the same extent as a commercial solution used to control erosive tooth wear (ETW).

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Copper molybdate nanoplates were synthesized by a sonochemical process at room temperature, which we report as a simple and cost-effective route. Structural analysis of the material by the Rietveld method of X-ray diffraction (XRD) data revealed lindgrenite Cu3(MoO4)2(OH)2 in a single-phase structure. All the vibrational modes characteristic of the space group were identified by Raman vibrational and near-infrared (NIR) spectroscopies. The profile obtained for N2 adsorption/desorption was type III hysteresis, characteristic of mesoporous materials, with a surface area of 70.77(1) m2 g-1. The micrographs of the material obtained by scanning electron microscopy showed nanoplates with nanometric sizes and an anisotropic growth aspect. The catalytic activity of lindgrenite was evaluated by esterifying oleic acid with methanol, showing high conversion rate to methyl oleate and good catalyst stability after seven recycling cycles. Above all, the best catalytic performance was reached when we optimized parameters such as oleic acid:methanol molar ratio of 1:5, 5% of catalyst dosage, and reaction time of 5 h, resulting in 98.38% of conversion at 413 K. Therefore, sonochemically synthesized lindgrenite proved to be a high potential material for biofuel production by oleic acid esterification.

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OBJECTIVE: This cross-sectional study evaluated the prevalence of tooth enamel defects and risk factors associated in primary dentition. METHODS: A sample of 656 schoolchildren (population based), aged 4 years old, from Araraquara-SP (Brazil) was evaluated by two trained examiners according to the following indexes: Dental fluorosis (DF), deciduous molar hypomineralization (DMH), non-fluoride related developmental defects of enamel (DDE), tooth erosion and tooth attrition. Structured questionnaires identified socioeconomic condition, medical/dental history, behavior and dietary habits. Data were analyzed using Chi-square tests (p < 0.05). RESULTS: Of the enamel congenital defects, DF was the most prevalent (6.1%, n = 40), followed by DMH (5.6%, n = 37). Of the acquired defects, attrition was the most prevalent (36.9%, n = 242), followed by erosion (2.4%, n = 16). The caries experience was similar between the affected children (29,5%; n = 94) and total sample (30.6%; n = 201). The etiological factors related to congenital defects were: not exclusively fed breast milk (p = 0.003) and jaundice at birth (p < 0.001); the association with acquired defects was found with vomiting frequent episodes (p = 0.037). CONCLUSION: The general prevalence of enamel defects in primary dentition in Araraquara was 48.6%. Enamel defects may be associated with health-related factors and current lifestyle.

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Dioctophymosis is the disease caused by Dioctophyma renale, a large nematode, popularly known as giant kidney worm, and whose definitive hosts are the domestic dog and many wild mammal species. There are some reports of maned wolf parasitism by D. renale, however, in most cases the reports are restricted to the finding of the parasite during necropsy, without clinical assessment. The present report aimed to describe the clinical-therapeutic, ultrasonographic and pathological aspects of D. renale parasitism in a free-ranging maned wolf, emphasizing the treatment with an association of doramectin, praziquantel, pyrantel pamoate, and febantel that resulted in complete elimination of the parasite.(AU)

A dioctofimose é a doença causada pelo Dioctophyma renale, um nemátodo grande, popularmente chamado de verme gigante do rim e que tem como hospedeiro definitivo o cão doméstico e inúmeras espécies de mamíferos silvestres. Existem alguns relatos do parasitismo do lobo-guará por D. renale, contudo, na maioria das vezes, os relatos se restringem apenas ao achado do parasita durante a necropsia, sem a correlação clínica. O presente relato objetiva descrever os aspectos clínico-terapêuticos, ultrassonográficos e patológicos do parasitismo por D. renale em um lobo-guará de vida livre, enfatizando o tratamento com uma associação de doramectina, praziquantel, pamoato de pirantel e febantel, o que resultou na eliminação completa do parasita.(AU)

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Objetivou-se avaliar o efeito de níveis de substituição de milho grão por polpa cítrica peletizada (0, 15 e 30%) em cordeiros confinados, sobre o consumo de matéria seca, conversão alimentar, ganho médio diário, rendimento de carcaça e custo diário com o arraçoamento. Foram utilizados 12 cordeiros do cruzamento das raças Ile de France e Suffolk, não castrados, com idade aproximada de 70 dias e peso vivo médio de 17,5 kg, distribuídos em delineamento inteiramente casualizado com quatro repetições, totalizando quatro animais por tratamento. Os animais foram alojados em baias individuais com 2,20 m2 , com comedouros e bebedouros individualizados. As dietas foram formuladas para serem isoproteicas e isoenergéticas, com teor de proteína bruta (PB) de 12,88% e nutrientes digestíveis totais (NDT) de 81,85%. O peso vivo final dos cordeiros que receberam dieta com 30% de substituição do milho grão por polpa cítrica peletizada foram superiores (p <0,05 ) aos obtidos em cordeiros com dieta sem inclusão de polpa cítrica. Os cordeiros que receberam dieta com 30% de polpa cítrica apresentaram maiores consumo de matéria seca em kg e em % de peso vivo em relação aos alimentados com dieta sem a inclusão de polpa (p 0,05). O ganho médio diário não foi influenciado pelos tratamentos. O custo com a alimentação (R$/dia) foi maior nos cordeiros que receberam a dieta com 30% de polpa cítrica em relação aos que receberam a dieta sem o coproduto. A substituição do milho grão por polpa cítrica peletizada em 30% proporcionou melhor rendimento de carcaça em relação ao tratamento sem a inclusão do coproduto, o que gerou maior receita com a venda das carcaças.(AU)

The objective of this work was to evaluate the effect of different levels of substitution of grain corn for citrus pulp (0, 15 and 30%) in confined lambs on dry matter intake, feed conversion, average daily gain, carcass yield and daily cost of feeding. Twelve non-castrated Ile de France and Suffolk crossbred lambs, approximately 70 days old and average live weight of 17.5 kg, were distributed in a completely randomized design, totaling four animals per treatment. The animals were housed in individual 2.20 m2 pens, with individual feeders and drinkers. The diets were formulated to be isoproteic and isoenergetic, with crude protein (CP) content of 12.88% and total digestible nutrients (TDN) of 81.85%. The live weight of lambs fed 30% of corn grain replacement by pelletized citrus pulp was higher (p <0.05) than of lambs with diet without citrus pulp. Lambs fed a diet with 30% citrus pulp had higher dry matter intake (kg) and dry matter intake (% body weight) compared to diet without citrus pulp (p <0.05). The average daily gain was not influenced by the treatments. The cost of food (R$/day) was higher in lambs who received a diet with 30% citrus pulp compared to those who received a diet without citrus pulp. Replacing maize grain by 30% pelleted citrus pulp offers better carcass yield about treatment without inclusion of citrus pulp, which generated higher revenue from the sale of carcasses.(AU)

Asunto(s)

RESUMEN

Objetivou-se avaliar o efeito de níveis de substituição de milho grão por polpa cítrica peletizada (0, 15 e 30%) em cordeiros confinados, sobre o consumo de matéria seca, conversão alimentar, ganho médio diário, rendimento de carcaça e custo diário com o arraçoamento. Foram utilizados 12 cordeiros do cruzamento das raças Ile de France e Suffolk, não castrados, com idade aproximada de 70 dias e peso vivo médio de 17,5 kg, distribuídos em delineamento inteiramente casualizado com quatro repetições, totalizando quatro animais por tratamento. Os animais foram alojados em baias individuais com 2,20 m2, com comedouros e bebedouros individualizados. As dietas foram formuladas para serem isoproteicas e isoenergéticas, com teor de proteína bruta (PB) de 12,88% e nutrientes digestíveis totais (NDT) de 81,85%.  O peso vivo final dos cordeiros que receberam dieta com 30% de substituição do milho grão por polpa cítrica peletizada foram superiores (p<0,05) aos obtidos em cordeiros com dieta sem inclusão de polpa cítrica. Os cordeiros que receberam dieta com 30% de polpa cítrica apresentaram maiores consumo de matéria seca em kg e em % de peso vivo em relação aos alimentados com dieta sem a inclusão de polpa (p<0,05). O ganho médio diário não foi influenciado pelos tratamentos. O custo com a alimentação (R$/dia) foi maior nos cordeiros que receberam a dieta com 30% de polpa cítr

RESUMEN

Objetivou-se avaliar o efeito de níveis de substituição de milho grão por polpa cítrica peletizada (0, 15 e 30%) em cordeiros confinados, sobre o consumo de matéria seca, conversão alimentar, ganho médio diário, rendimento de carcaça e custo diário com o arraçoamento. Foram utilizados 12 cordeiros do cruzamento das raças Ile de France e Suffolk, não castrados, com idade aproximada de 70 dias e peso vivo médio de 17,5 kg, distribuídos em delineamento inteiramente casualizado com quatro repetições, totalizando quatro animais por tratamento. Os animais foram alojados em baias individuais com 2,20 m2 , com comedouros e bebedouros individualizados. As dietas foram formuladas para serem isoproteicas e isoenergéticas, com teor de proteína bruta (PB) de 12,88% e nutrientes digestíveis totais (NDT) de 81,85%. O peso vivo final dos cordeiros que receberam dieta com 30% de substituição do milho grão por polpa cítrica peletizada foram superiores (p <0,05 ) aos obtidos em cordeiros com dieta sem inclusão de polpa cítrica. Os cordeiros que receberam dieta com 30% de polpa cítrica apresentaram maiores consumo de matéria seca em kg e em % de peso vivo em relação aos alimentados com dieta sem a inclusão de polpa (p 0,05). O ganho médio diário não foi influenciado pelos tratamentos. O custo com a alimentação (R$/dia) foi maior nos cordeiros que receberam a dieta com 30% de polpa cítrica em relação aos que receberam a dieta sem o coproduto. A substituição do milho grão por polpa cítrica peletizada em 30% proporcionou melhor rendimento de carcaça em relação ao tratamento sem a inclusão do coproduto, o que gerou maior receita com a venda das carcaças.

The objective of this work was to evaluate the effect of different levels of substitution of grain corn for citrus pulp (0, 15 and 30%) in confined lambs on dry matter intake, feed conversion, average daily gain, carcass yield and daily cost of feeding. Twelve non-castrated Ile de France and Suffolk crossbred lambs, approximately 70 days old and average live weight of 17.5 kg, were distributed in a completely randomized design, totaling four animals per treatment. The animals were housed in individual 2.20 m2 pens, with individual feeders and drinkers. The diets were formulated to be isoproteic and isoenergetic, with crude protein (CP) content of 12.88% and total digestible nutrients (TDN) of 81.85%. The live weight of lambs fed 30% of corn grain replacement by pelletized citrus pulp was higher (p <0.05) than of lambs with diet without citrus pulp. Lambs fed a diet with 30% citrus pulp had higher dry matter intake (kg) and dry matter intake (% body weight) compared to diet without citrus pulp (p <0.05). The average daily gain was not influenced by the treatments. The cost of food (R$/day) was higher in lambs who received a diet with 30% citrus pulp compared to those who received a diet without citrus pulp. Replacing maize grain by 30% pelleted citrus pulp offers better carcass yield about treatment without inclusion of citrus pulp, which generated higher revenue from the sale of carcasses.

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RESUMEN

A febre maculosa brasileira (FMB), descrita inicialmente nos Estados Unidos como febre maculosa das Montanhas Rochosas, é uma antropozoonose relatada apenas no continente americano e causada pela bactéria Rickettsia rickettsii. No Brasil a transmissão ocorre sobretudo pela picada de carrapatos do gênero Amblyomma spp. A doença foi inicialmente descrita como de transmissão em áreas rurais e silvestres, no entanto áreas periurbanas e urbanas vêm apresentando casos, principalmente relacionados com a presença de humanos residindo em pequenos fragmentos de mata ciliar. O presente estudo teve por objetivo elucidar a dispersão da FMB nas proximidades dos reservatórios Guarapiranga e Billings, na cidade de São Paulo, SP. Para tanto, a presença de anticorpos anti-R. rickettsii, Rickettsia parkeri e Rickettsia bellii foi avaliada em cães atendidos nas campanhas de esterilização cirúrgica e residentes ao redor dos reservatórios. Foram coletadas amostras de 393 cães, e as amostras de soro foram analisadas pela reação de imunofluorescência indireta (RIFI), com ponto de corte de 1:64. Os títulos para R. rickettsii variaram de 256 a 4096, com positividade de 3,3% (13/393); para R. bellii, de 128 a 1024 e 4,1% (16/393) de positivos, e um único animal (0,25%) foi soropositivo para R. parkeri, com título de 128. Os achados permitem concluir que a região de estudo apresenta condições de se tornar uma possível área com casos de FMB, pois comporta fragmentação de Mata Atlântica, condições essas ideais para a manutenção do vetor do gênero Amblyomma já descrito na região, bem como para a presença da Rickettsia rickettsii circulante entre os cães, confirmada pela existência de anticorpos. Condutas referentes à conscientização da população por meio de trabalhos educacionais devem ser implantadas para a prevenção da doença na população da área.(AU)

Brazilian Spotted Fever (BSF), initially described in the United States as Rocky Mountain Spotted Fever, is an anthropozoonosis reported only in the Americas and caused by the bacterium Rickettsia rickettsii. In Brazil, transmission occurs mainly through tick bites of the genus Amblyomma spp. The disease was initially described as transmission of rural and wild areas; however, peri-urban and urban areas have been presenting cases, mainly related to the presence of humans residing in small fragments of riparian forest. The present study aimed to elucidate the dispersal of BSF near the Guarapiranga and Billings Reservoirs, in the city of São Paulo, SP. The presence of anti-R. rickettsii, Rickettsia parkeri and Rickettsia bellii antibodies were evaluated in dogs treated in surgical sterilization campaigns and residents around the Reservoirs. Samples were collected from 393 dogs and serum samples were analyzed by indirect immunofluorescence reaction (RIFI) with a cutoff of 1:64. The titles for R. rickettsii varied from 256 to 4096 with a positivity of 3.3% (13/393); for R. bellii from 128 to 1024 and 4.1% (16/393) of positive and a single animal (0.25%) was seropositive for R. parkeri with a titre of 128. The findings allow us to conclude that the study region has conditions to become a possible area with BSF cases, as it involves Atlantic Forest, ideal conditions for the maintenance of the vector of the genus Amblyomma already described in the region and the presence of circulating Rickettsia rickettsii among dogs, confirmed by the presence of antibodies. Conducts regarding the awareness of the population through educational work should be implemented to prevent the disease in the population of the area.(AU)

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