RESUMEN
The interplay between cervical cancer (CC) and immune cells, mainly intratumoral lymphocytes, has a pivotal role in carcinogenesis. In this context, we evaluated the distribution of CD45RA+ and CD45RO+ cells as well as CCR6+ and CCL20+ cells in intraepithelial (IE) and marginal stroma (MS) areas from cervical intraepithelial neoplasia (CIN) I-III, and CC as 'immunoscore' for HPV-induced CC outcome. We observed increased CD45RA+ and CD45RO+ cells distribution in IE and MS areas in the CC group compared to CIN groups and healthy volunteers. Interestingly, there is a remarkable reduction of CCL20+ expressing cells distribution according to lesion severity. The CC group had a significant decrease in CCL20+ and CCR6+-expressing cells distribution in both IE and MS areas compared to all groups. Using the 'immunoscore' model, we observed an increased number of women presenting high CD45RA+/CD45RO+ and low CCL20+/CCR6+ 'immunoscore' in the CC group. Our results suggested a pattern in cervical inflammatory process with increasing CD45RA+/CD45RO+, and decreasing CCL20+/CCR6+ expression in accordance with CIN severity. Taken together, these markers could be evaluated as 'immunoscore' predictors to CC response. A more comprehensive analysis of longitudinal studies should be conducted to associate CD45RA+/CD45RO+ and CCL20+/CCR6+ 'immunoscore' to CC progression and validate its value as a prognosis method.
Asunto(s)
Alphapapillomavirus/patogenicidad , Antígenos Comunes de Leucocito/inmunología , Infecciones por Papillomavirus/inmunología , Neoplasias del Cuello Uterino/inmunología , Adulto , Estudios de Casos y Controles , Quimiocina CCL20 , Femenino , Humanos , Infecciones por Papillomavirus/patología , Infecciones por Papillomavirus/virología , Pronóstico , Receptores CCR6 , Neoplasias del Cuello Uterino/patología , Neoplasias del Cuello Uterino/virologíaRESUMEN
Avalia o emprego de métodos moleculares a fim de comprovar a presença dos HPV no trato genital para utilização em combinação com a citopatologia, método utilizado para rastreamento das lesões causadas pelo HPV
Asunto(s)
Humanos , Femenino , Adolescente , Adulto , Sondas de ADN de HPV , Neoplasias Pélvicas/diagnóstico , Papillomaviridae , Enfermedades de Transmisión SexualRESUMEN
Human Papillomavirus (HPV) infection is the main cause of cervical cancers and cervical intraepithelial neoplasias (CIN) worldwide. Consequently, it would be useful to evaluate HPV testing to screen for cervical cancer. Recently developed, the second-generation Hybrid Capture (HCA II) test is a non-radioactive, relatively rapid, liquid hybridization assay designed to detect 18 HPV types, divided into high and low-risk groups. We evaluated 1055 women for HPV infection with the HCA II test. Five hundred and ten (48.3%) of these women had HPV infection; 60 (11.8%) had low cancer-risk HPV DNA; 269 (52.7%) had high-risk HPV types and 181 (35.5%) had both groups. Hence, 450 women (88.2%) in this HPV-infected group had at least one high risk HPV type, and were therefore considered to be at high risk for cancer. Among the group with Papanicolaou (Pap) test results, the overall prevalence of HPV DNA was 58.4%. Significant differences in HPV infection of the cervix were detected between Pap I (normal smears) and Pap IV (carcinomas) (p<0.0001). Values of HPV viral load obtained for Pap I and SILs were significantly different, with an upward trend (p<0.0001), suggesting a positive correlation between high viral load values and risk of SIL. Because of the high costs of the HCA II test, its use for routine cervical mass screening cannot be recommended in poor countries. Nevertheless, it is a useful tool when combined with cytology, diagnosing high-risk infections in apparently normal tissues. Use of this technique could help reduce the risk of cancer.
Asunto(s)
ADN Viral/análisis , Hibridación de Ácido Nucleico/métodos , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/diagnóstico , Neoplasias del Cuello Uterino/virología , Adulto , Distribución por Edad , Femenino , Humanos , Persona de Mediana Edad , Prueba de Papanicolaou , Papillomaviridae/genética , Infecciones por Papillomavirus/complicaciones , Factores de Riesgo , Neoplasias del Cuello Uterino/prevención & control , Frotis Vaginal , Carga ViralRESUMEN
Human Papillomavirus (HPV) infection is the main cause of cervical cancers and cervical intraepithelial neoplasias (CIN) worldwide. Consequently, it would be useful to evaluate HPV testing to screen for cervical cancer. Recently developed, the second-generation Hybrid Capture (HCA II) test is a non-radioactive, relatively rapid, liquid hybridization assay designed to detect 18 HPV types, divided into high and low-risk groups. We evaluated 1055 women for HPV infection with the HCA II test. Five hundred and ten (48.3 percent) of these women had HPV infection; 60 (11.8 percent) had low cancer-risk HPV DNA; 269 (52.7 percent) had high-risk HPV types and 181 (35.5 percent) had both groups. Hence, 450 women (88.2 percent) in this HPV-infected group had at least one high risk HPV type, and were therefore considered to be at high risk for cancer. Among the group with Papanicolaou (Pap) test results, the overall prevalence of HPV DNA was 58.4 percent. Significant differences in HPV infection of the cervix were detected between Pap I (normal smears) and Pap IV (carcinomas) (p<0.0001). Values of HPV viral load obtained for Pap I and SILs were significantly different, with an upward trend (p<0.0001), suggesting a positive correlation between high viral load values and risk of SIL. Because of the high costs of the HCA II test, its use for routine cervical mass screening cannot be recommended in poor countries. Nevertheless, it is a useful tool when combined with cytology, diagnosing high-risk infections in apparently normal tissues. Use of this technique could help reduce the risk of cancer
Asunto(s)
Humanos , Femenino , Adulto , Persona de Mediana Edad , Displasia del Cuello del Útero , ADN Viral , Hibridación de Ácido Nucleico , Papillomaviridae , Infecciones por Papillomavirus/patología , Neoplasias del Cuello Uterino , Distribución por Edad , Brasil , Displasia del Cuello del Útero , Papillomaviridae , Prevalencia , Infecciones por Papillomavirus/complicaciones , Infecciones por Papillomavirus/epidemiología , Factores de Riesgo , Neoplasias del Cuello Uterino , Frotis Vaginal , Carga ViralRESUMEN
Estudo das infecçöes genitais causadas pelo HPV, determinando a prevalência dos tipos de HPV em pacientes do sexo feminino, atendidas pelo Laboratório Sérgio Franco do Estado do Rio de Janeiro, no ano de 2001, pela técnica de captura do híbrido