RESUMEN
An in vitro study was carried out to determine the IgM response to staphylococcal antigens (Staphylococcus aureus, serotype 3, ATCC 12600), at different stages of the growth curve. This was in order to observe the number of inhibited haemolytic plaques when immunosera obtained from blood-letting in rabbits previously immunized with antigenic components were used.
Asunto(s)
Anticuerpos Antibacterianos/biosíntesis , Antígenos Bacterianos/inmunología , Inmunoglobulina M/biosíntesis , Staphylococcus aureus/inmunología , Animales , Células Productoras de Anticuerpos/análisis , Técnica de Placa Hemolítica , Sueros Inmunes/inmunología , Ratones , Conejos , Staphylococcus aureus/crecimiento & desarrolloRESUMEN
IgG antibodies against E. granulosus were investigated in order to determine the true prevalence of human echinococcosis in Badajoz, Spain. The seroepidemiological survey was carried out on 1540 subjects not suspected of suffering from hydatidosis, in order to add this morbidity rate to that already declared. Because of its high sensitivity and specificity, a standardized immunoenzymatic technique, ELISA was used, and the results subsequently compared using a classical technique for seroepidemiological surveys, namely indirect hemagglutination test (IHA). According to ELISA, 2.2% of the tested subjects were positive. This indicates a higher morbidity in our province than that declared. This test was found to be ideal for seroepidemiological surveys since it is reproducible, easily and rapidly conducted and safe. It requires economical reagents, a large number of samples can be processed simultaneously and it offers a totally objective reading.