RESUMEN
Broomrapes (Orobanche and Phelipanche spp) are parasitic plants responsible for important crop losses, and efficient procedures to control these pests are scarce. Biological control is one of the possible strategies to tackle these pests. Arbuscular Mycorrhizal (AM) fungi are widespread soil microorganisms that live symbiotically with the roots of most plant species, and they have already been tested on sorghum for their ability to reduce infestation by witchweeds, another kind of parasitic plants. In this work AM fungi were evaluated as potential biocontrol agents against Orobanche cumana, a broomrape species that specifically attacks sunflower. When inoculated simultaneously with O. cumana seeds, AM fungi could offer a moderate level of protection against the broomrape. Interestingly, this protection did not only rely on a reduced production of parasitic seed germination stimulants, as was proposed in previous studies. Rather, mycorrhizal root exudates had a negative impact on the germination of O. cumana induced by germination stimulants. A similar effect could be obtained with AM spore exudates, establishing the fungal origin of at least part of the active compounds. Together, our results demonstrate that AM fungi themselves can lead to a reduced rate of parasitic seed germination, in addition to possible effects mediated by the mycorrhizal plant. Combined with the other benefits of AM symbiosis, these effects make AM fungi an attractive option for biological control of O. cumana.
Asunto(s)
Agentes de Control Biológico , Germinación , Micorrizas/fisiología , Orobanche/crecimiento & desarrollo , Semillas/crecimiento & desarrollo , Helianthus/metabolismo , Helianthus/microbiología , Helianthus/parasitología , Micorrizas/metabolismo , Orobanche/microbiología , Raíces de Plantas/metabolismo , Raíces de Plantas/microbiología , Raíces de Plantas/parasitología , Semillas/microbiología , Esporas Fúngicas/metabolismo , Esporas Fúngicas/fisiologíaRESUMEN
In tobacco, 9-divinyl ethers (DVEs) produced by the lipoxygenase NtLOX1 and DVE synthase NtDES1 are important for full resistance to pathogens. In this work, the regulation of NtLOX1 and NtDES1 expression by signal molecules was investigated in LOX1 promoter-reporter transgenic plants and by RT-qPCR. Methyl jasmonate, ACC and elicitor were shown to coordinately trigger the DVE pathway. Induction was strongly attenuated in the presence of salicylic acid, which seems to act as a negative regulator of the 9-DVE biosynthetic enzymes. Our data suggest that, in tobacco, DVE biosynthesis is cross-regulated by jasmonates, and by other hormonal and signal molecules such as ethylene and SA.
Asunto(s)
Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Inmunidad Innata/genética , Nicotiana/genética , Enfermedades de las Plantas/genética , Proteínas de Plantas/genética , Compuestos de Vinilo/metabolismo , Acetatos/metabolismo , Aminoácidos Cíclicos/metabolismo , Ciclopentanos/metabolismo , Sistema Enzimático del Citocromo P-450/genética , Sistema Enzimático del Citocromo P-450/metabolismo , Lipooxigenasa/genética , Oxilipinas/metabolismo , Phytophthora , Reguladores del Crecimiento de las Plantas/metabolismo , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Ácido Salicílico/metabolismo , Transducción de Señal/genética , Nicotiana/metabolismoRESUMEN
Plant oxylipins are a large family of metabolites derived from polyunsaturated fatty acids. The characterization of mutants or transgenic plants affected in the biosynthesis or perception of oxylipins has recently emphasized the role of the so-called oxylipin pathway in plant defense against pests and pathogens. In this context, presumed functions of oxylipins include direct antimicrobial effect, stimulation of plant defense gene expression, and regulation of plant cell death. However, the precise contribution of individual oxylipins to plant defense remains essentially unknown. To get a better insight into the biological activities of oxylipins, in vitro growth inhibition assays were used to investigate the direct antimicrobial activities of 43 natural oxylipins against a set of 13 plant pathogenic microorganisms including bacteria, oomycetes, and fungi. This study showed unequivocally that most oxylipins are able to impair growth of some plant microbial pathogens, with only two out of 43 oxylipins being completely inactive against all the tested organisms, and 26 oxylipins showing inhibitory activity toward at least three different microbes. Six oxylipins strongly inhibited mycelial growth and spore germination of eukaryotic microbes, including compounds that had not previously been ascribed an antimicrobial activity, such as 13-keto-9(Z),11(E),15(Z)-octadecatrienoic acid and 12-oxo-10,15(Z)-phytodienoic acid. Interestingly, this first large-scale comparative assessment of the antimicrobial effects of oxylipins reveals that regulators of plant defense responses are also the most active oxylipins against eukaryotic microorganisms, suggesting that such oxylipins might contribute to plant defense through their effects both on the plant and on pathogens, possibly through related mechanisms.