RESUMEN

Genotyping and molecular characterization of drug resistance mechanisms in Mycobacterium leprae enables disease transmission and drug resistance trends to be monitored. In the present study, we performed genome-wide analysis of Airaku-3, a multidrug-resistant strain with an unknown mechanism of resistance to rifampicin. We identified 12 unique non-synonymous single-nucleotide polymorphisms (SNPs) including two in the transporter-encoding ctpC and ctpI genes. In addition, two SNPs were found that improve the resolution of SNP-based genotyping, particularly for Venezuelan and South East Asian strains of M. leprae.

Asunto(s)

Farmacorresistencia Bacteriana Múltiple , Mycobacterium leprae/genética , Análisis de Secuencia de ADN/métodos , Asia Sudoriental , Genoma Bacteriano , Genotipo , Humanos , Lepra/microbiología , Datos de Secuencia Molecular , Mycobacterium leprae/clasificación , Filogenia , Polimorfismo de Nucleótido Simple , Venezuela