RESUMEN
Dengue virus is the most significant virus transmitted by arthropods worldwide and may cause a potentially fatal systemic disease named dengue hemorrhagic fever. In this work, dengue virus serotype 4 was detected in the tissues of one fatal dengue hemorrhagic fever case using electron immunomicroscopy and molecular methods. This is the first report of dengue virus polypeptides findings by electron immunomicroscopy in human samples. In addition, not-previously-documented virus-like particles visualized in spleen, hepatic, brain, and pulmonary tissues from a dengue case are discussed.
Asunto(s)
Virus del Dengue/genética , Virus del Dengue/ultraestructura , Dengue Grave/diagnóstico , Adulto , Anticuerpos Antivirales/sangre , Encéfalo/ultraestructura , Encéfalo/virología , Cuba , ADN Viral/análisis , Virus del Dengue/aislamiento & purificación , Ensayo de Inmunoadsorción Enzimática , Resultado Fatal , Femenino , Corazón/virología , Humanos , Inmunoglobulina M/sangre , Riñón/ultraestructura , Riñón/virología , Hígado/ultraestructura , Hígado/virología , Microscopía Electrónica de Transmisión/métodos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Dengue Grave/virología , Bazo/ultraestructura , Bazo/virologíaRESUMEN
El género Mycobacterium provoca infecciones pulmonares y extrapulmonares, de estas últimas predomina la infección ganglionar. Mientras Mycobacterium tuberculosis es el agente causal más importante, en las últimas décadas aumenta la incidencia de otras especies micobacterianas que se han hecho prevalentes en los pacientes positivos al virus de la inmunodeficiencia humana (VIH +) tanto en países desarrollados como en vías de desarrollo. Durante el período enero 2007 hasta diciembre 2009 se procesó en nuestro laboratorio 6540 muestras, 210 muestras fueron obtenidas por biopsia ganglionar, precisamente este constituyó nuestro universo de estudio, 190 (90.4%) muestras se obtuvieron por exéresis quirúrgica, 20 (9.5%) por punción espirativa;17 procedían de pacientes VIH (8.1%) y 193 procedentes de pacientes VIH+(91.9%). En solo 16 muestras (7.6%) el cultivo BAAR fue positivo; 4 procedentes de pacientes VIH (25%) y 12 VIH+(75%). La clasificación e identificación micobacteriana demostró la presencia de Mycobacterium tuberculosis en 13 de los casos (81.25%), mientras Mycobacterium avium-intracellulare fue aislado en 3 (18.7%). En los pacientes inmunodeprimidos con linfadenopatía incluidos los pacientes VIH/sida, es muy importante la búsqueda activa de la presencia de BAAR como coinfección oportunista, donde Mycobacterium tuberculosis se mantiene como el agente infeccioso más frecuente, sin embargo la posibilidad de que otras especies micobacterianas también estén presentes no se debe descartar. Nuestro objetivo en este estudio como Laboratorio Nacional de Referencia de TB- Micobacterias fue lograr la caracterización etiológica de linfadenopatías en pacientes en que se sospechaba clínicamente la participación del género Mycobacterium.
Mycobacterium tuberculosis is the most important etiological agent producing pulmonary as well as extrapulmonary infection. During these last decades, the increase in the incidence of infection due to other mycobacteria species is evident. Lymphadenopathy is the most frequent extrapulmonary presentation form of Mycobacterium Genera infection among HIV positive patients either in developed or underdeveloped countries. The aim of this work is to analyze the results obtained during January 2007 - December 2009 in our laboratory. Two hundred ten tissue samples were studied; 190 (90.4%) samples were lymph node biopsied tissues and 20 (9.5%) samples were obtained by fine needle aspiration; 17 were from HIV - patients (8.1%) and 193 from HIV + (91.9%). A total of 16 (7.6%) samples produced a positive culture for BAAR, 4 VIH- (25%) and 12 VIH+ (75%). Classification and identification for mycobacteria confirmed Mycobacterium tuberculosis in 13 of the cases (81.25%), and Mycobacterium avium-intracellulare in three patients (18.7%). The present study once again confirms that BAAR culture has more sensitivity and specificity than histopathologhic studies have. Lymphadenopathy in immunosuppressed patients should by studied for the presence of an BAAR coinfection where M. tuberculosis is still the agent most frequently found, nevertheless, other species of Mycobacteria may be causing infection and should be searched for. Our objective as National Reference Laboratory of Tuberculosis and Mycobacterial was to obtain the etiological characterization of Mycobacterium lymphadenopathy in clinically suspect patients.
Asunto(s)
Humanos , Complejo Mycobacterium avium/patogenicidad , Enfermedades Pulmonares Fúngicas/complicaciones , VIH , Linfadenitis/patología , Mycobacterium tuberculosis/patogenicidad , Síndrome de Inmunodeficiencia Adquirida/complicacionesRESUMEN
Dengue fever (DF) and dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) are considered the most important arthropod-borne viral diseases in terms of morbidity and mortality. The emergency and severity of dengue (Den) infections increase the necessity of an early, quick and effective dengue laboratory diagnostic. Viral isolation is considered a gold standard for diagnosis of dengue infection using monoclonal antibodies (mAbs) as a tool for determining serotype specificity. Alternatives have been used to improve sensitivity and time to dengue diagnosis. Based on the early expression of dengue C protein in the life cycle, we focused our study on the application of an anti-dengue 2 virus capsid protein mAb in dengue diagnosis. The kinetic expression of dengue-2 capsid in mosquito cells and its immuno-localization in experimentally infected suckling albin Swiss (OF-1) mice brain tissues was established. The results demonstrate the possible utility of this mAb in early dengue diagnosis versus traditional isolation. In addition, a preliminary study of an enzyme immunoassay method using 8H8 mAb for specific detection of dengue C protein antigen was performed, making possible recombinant C protein quantification. The results suggest that detection of dengue capsid protein could be useful in the diagnosis of early dengue infection.
Asunto(s)
Anticuerpos Monoclonales/inmunología , Anticuerpos Antivirales/inmunología , Proteínas de la Cápside/inmunología , Virus del Dengue/inmunología , Dengue/diagnóstico , Dengue Grave/diagnóstico , Aedes , Animales , Animales Lactantes , Antígenos Virales/inmunología , Línea Celular , Dengue/inmunología , Humanos , Técnicas para Inmunoenzimas , Ratones , Dengue Grave/inmunologíaRESUMEN
A cross-sectional study was performed in 60 Cuban women of child-bearing age who were seropositive for human immunodeficiency virus (HIV) and 60 controls. Human papillomavirus (HPV) was identified most frequently, with oncogenic HPV serotypes 16, 33 and 58 detected in HIV-positive patients, and serotypes 11, 33 and 51 in the controls (relative risk 4.41; 95% CI 2.21-8.29). Syphilis and hepatitis B and C viruses were detected exclusively in HIV-seropositive women (p<0.05). Sexually transmitted diseases (STDs) appeared to pose a substantial health problem, especially for HIV-positive women. Clinics should consider screening and treatment for STDs as part of their HIV prevention programmes.
Asunto(s)
Papillomaviridae , Enfermedades de Transmisión Sexual/epidemiología , Adolescente , Adulto , Comorbilidad , Estudios Transversales , Cuba/epidemiología , Femenino , Seropositividad para VIH/epidemiología , Hepatitis B/epidemiología , Hepatitis C/epidemiología , Humanos , Persona de Mediana Edad , Infecciones por Papillomavirus/epidemiología , Prevalencia , Sífilis/epidemiologíaRESUMEN
From April to September 2000, an epidemic of aseptic meningitis spread throughout Cuba, with 16,943 reported cases. Virologic studies identified echovirus 16 as the cause of this epidemic. This is the first reported isolate of echovirus 16 from patients with viral meningitis in Cuba.
Asunto(s)
Brotes de Enfermedades , Infecciones por Echovirus/epidemiología , Enterovirus Humano B/aislamiento & purificación , Meningitis Aséptica/epidemiología , Meningitis Viral/epidemiología , Adolescente , Adulto , Distribución por Edad , Líquido Cefalorraquídeo/virología , Niño , Preescolar , Cuba/epidemiología , Infecciones por Echovirus/diagnóstico , Infecciones por Echovirus/virología , Enterovirus Humano B/clasificación , Enterovirus Humano B/genética , Heces/virología , Humanos , Incidencia , Lactante , Meningitis Aséptica/diagnóstico , Meningitis Aséptica/virología , Meningitis Viral/diagnóstico , Meningitis Viral/virología , Persona de Mediana Edad , ARN Viral/análisis , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
During the epidemic of optic and peripheral neuropathy which occurred in Cuba in 1992-1993, viruses antigenically related to the Coxsackie viruses were isolated from cerebrospinal fluid of patients. Concurrently with the virologic studies, epidemiologic, toxicologic, nutritional, immunologic, and histopathologic investigations were also carried out. Although it was demonstrated that the illness was associated with toxic and nutritional risk factors, it has not been possible to identify a specific etiology for the symptoms observed. Taking into consideration the results obtained in all of the various investigations, we have formulated an integral, multifactorial hypothesis which attempts to explain a pathophysiologic mechanism by which the viruses isolated could participate in the pathogenesis of the illness. We propose that the viral agents produce a persistent infection, and the possibility that they may act as mediator of an autoimmune pathogenic process.
Asunto(s)
Infecciones por Coxsackievirus/epidemiología , Infecciones por Coxsackievirus/fisiopatología , Enterovirus/aislamiento & purificación , Enfermedades del Nervio Óptico/epidemiología , Enfermedades del Sistema Nervioso Periférico/epidemiología , Infecciones por Coxsackievirus/inmunología , Cuba/epidemiología , Humanos , Modelos Biológicos , Modelos Neurológicos , Enfermedades del Nervio Óptico/fisiopatología , Enfermedades del Nervio Óptico/virología , Enfermedades del Sistema Nervioso Periférico/fisiopatología , Enfermedades del Sistema Nervioso Periférico/virologíaRESUMEN
The L1 larval stage of Trichinella spiralis induces modification in a portion of striated skeletal muscle cell resulting in the formation of the nurse cell. This specialized host cell is completely encased in a capsule composed mainly of collagen type IV and type VI, which, in turn, is surrounded by a unique rete of vessels whose formation begins on around day 12 after intracellular infection. We investigated the possibility that vascular endothelial growth factor (VEGF) may be up-regulated during nurse cell formation by employing immunohistochemistry and in situ hybridization on synchronously infected mouse muscle tissue. Both VEGF mRNA and VEGF peptide were detected in the developing nurse cell cytoplasm from day 7 up to 16 mo after infection. In addition, VEGF was also detected in cells in the area immediately surrounding the nurse cell on days 15 and 17. On the basis of these results, we propose that hypoxia is induced by T. spiralis within the developing nurse cell some time prior to the up-regulation of VEGF, perhaps as early as day 7. We further propose, on the basis of the continued presence of VEGF in nurse cell cytoplasm, that a constant state of hypoxia cell is maintained.
Asunto(s)
Factores de Crecimiento Endotelial/metabolismo , Linfocinas/metabolismo , Músculo Esquelético/parasitología , Trichinella spiralis/fisiología , Animales , Factores de Crecimiento Endotelial/análisis , Factores de Crecimiento Endotelial/genética , Interacciones Huésped-Parásitos , Inmunohistoquímica , Hibridación in Situ , Larva/fisiología , Linfocinas/análisis , Linfocinas/genética , Ratones , Músculo Esquelético/citología , Músculo Esquelético/metabolismo , Reacción en Cadena de la Polimerasa , ARN Mensajero/análisis , Regulación hacia Arriba , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial VascularRESUMEN
OBJECTIVE: To investigate the possibility of a viral agent in the central nervous system of patients with epidemic neuropathy. DESIGN: Virus isolation attempts, in cell cultures and suckling mice, from cerebrospinal fluid (CSF) of neuropathy patients and controls undergoing lumbar puncture for unrelated reasons. Serologic studies in patients, contacts, and controls. SETTING: An epidemic of optic and peripheral neuropathy affected more than 50,000 people in Cuba in 1991 through 1993. Illness was associated with dietary limitations and increased physical demands accompanying the shortages of food and fuel experienced in Cuba since 1989. Most patients responded to parenteral vitamin therapy, and the epidemic began to subside when oral vitamin supplementation was begun for the entire Cuban population. RESULTS: Coxsackievirus A9 (five isolates) and a similar, less cytopathic virus (100 isolates) were recovered from 105 (84%) of 125 CSF specimens from neuropathy patients. The strains with light cytopathic effect were antigenically related to Coxsackieviruses A9 and B4 by cross-neutralization and immunoblotting assays. Virus persisted in CSF of some patients for 1 to 12 months. Cerebrospinal fluid from patients and both types of virus from cell culture produced illness, including complete posterior flaccid paralysis, in newborn mice, and virus was reisolated from the mice. Mouse tissues and sural nerve biopsy specimens from patients were stained by immunoperoxidase and colloidal gold techniques using hyperimmune rabbit antisera against the virus with light cytopathic effect. CONCLUSIONS: Coxsackievirus A9 or an antigenically related agent with a light cytopathic effect was present in CSF of 84% of 125 patients with epidemic neuropathy. The role of these agents, probably in combination with nutritional factors, in the pathophysiology of the disease requires further investigation.
Asunto(s)
Infecciones por Coxsackievirus/etiología , Brotes de Enfermedades , Enterovirus/aislamiento & purificación , Neuritis Óptica/virología , Enfermedades del Sistema Nervioso Periférico/virología , Adulto , Animales , Animales Lactantes/virología , Anticuerpos Antivirales/análisis , Antígenos Virales/análisis , Técnicas de Cultivo de Célula , Líquido Cefalorraquídeo/virología , Chlorocebus aethiops , Infecciones por Coxsackievirus/líquido cefalorraquídeo , Infecciones por Coxsackievirus/epidemiología , Infecciones por Coxsackievirus/patología , Cuba/epidemiología , Efecto Citopatogénico Viral , Enterovirus/inmunología , Enterovirus/patogenicidad , Femenino , Humanos , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos BALB C , Persona de Mediana Edad , Neuritis Óptica/líquido cefalorraquídeo , Neuritis Óptica/epidemiología , Neuritis Óptica/patología , Sistema Nervioso Periférico/patología , Sistema Nervioso Periférico/virología , Enfermedades del Sistema Nervioso Periférico/líquido cefalorraquídeo , Enfermedades del Sistema Nervioso Periférico/epidemiología , Enfermedades del Sistema Nervioso Periférico/patología , Conejos , Células Vero/virologíaRESUMEN
During the epidemic neuropathy occurred in Cuba from 1992 to 1993, viral isolations antigenically connected with Coxsackie viruses were obtained from the cerebrospinal fluid of patients. Virological, epidemiological, toxicologic, nutritional, immunological and histopathological investigations were made. Though the disease was related to toxic and nutritional factors, it has been impossible to identify the cause of the epidemic. Taking into consideration the results of the different investigations, we have formulated a comprehensive and multifactorial hypothesis to explain the physiopathological mechanism of the participation of the isolated viruses as mediators in a process of autoimmunity of the pathogeny of the disease.
Asunto(s)
Enfermedades Autoinmunes/etiología , Infecciones por Coxsackievirus/complicaciones , Brotes de Enfermedades , Enterovirus/patogenicidad , Enfermedades del Nervio Óptico/etiología , Enfermedades del Sistema Nervioso Periférico/etiología , Animales , Enfermedades Autoinmunes/líquido cefalorraquídeo , Enfermedades Autoinmunes/epidemiología , Enfermedades Autoinmunes/inmunología , Enfermedades Autoinmunes/virología , Líquido Cefalorraquídeo/virología , Infecciones por Coxsackievirus/epidemiología , Infecciones por Coxsackievirus/inmunología , Cuba/epidemiología , Efecto Citopatogénico Viral , Enterovirus/inmunología , Enterovirus/aislamiento & purificación , Enterovirus/fisiología , Humanos , Interleucina-2/biosíntesis , Ratones , Modelos Biológicos , Imitación Molecular , Proteínas del Tejido Nervioso/inmunología , Trastornos Nutricionales/complicaciones , Enfermedades del Nervio Óptico/líquido cefalorraquídeo , Enfermedades del Nervio Óptico/epidemiología , Enfermedades del Nervio Óptico/inmunología , Enfermedades del Nervio Óptico/virología , Enfermedades del Sistema Nervioso Periférico/líquido cefalorraquídeo , Enfermedades del Sistema Nervioso Periférico/epidemiología , Enfermedades del Sistema Nervioso Periférico/inmunología , Enfermedades del Sistema Nervioso Periférico/virología , Fumar/efectos adversos , Subgrupos de Linfocitos T/inmunología , Proteínas Virales/inmunología , Latencia del VirusRESUMEN
The epidemiological situation of bacterial meningitis is increasing dramatically. There is no doubt that the lack of proper animal models has hampered the achievement of effective prophylactic and therapeutic means. We report the characterization of the experimental disease caused by Haemophilus influenzae type b (Hib) in mice, taking into account its importance as an etiological agent of such a type of meningitis. The high resistance of C57BL/6, CBA/ J and BALB/cJ mice to Hib infection was proven. LD50 of Hib using trypsin or iron dextran as virulence enhancement factors (VEF), both being similar and more than 1000 times lower than that without any VEF, were determined. Lesions of CNS compatible with meningitis were found in about one third of specimens. Hair bristling, conjunctivitis, diarrhea, depression and prostration were the most characteristic symptoms. The proportion of animals which die is highest on the first day, lower on the second and almost zero after 48 h of infection. Water and food intake was higher in control than in infected animals; nevertheless, there were no differences in body weight increase among the mice after 5 days post-infection. Microorganisms were isolated from CSF and blood after 6 h of infection and positive results remained according to the size of the inoculum. Despite the acuteness of the experimental disease, antibiotic treatment with internationally recommended drugs was shown to be effective. Similar results were achieved when hyperimmune serum vs. Hib was applied.
Asunto(s)
Haemophilus influenzae , Meningitis por Haemophilus/microbiología , Animales , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Endogámicos CBARESUMEN
Isolated cases and outbreaks of infection with Trichinella spp. occur frequently throughout the world, sometimes resulting in fatalities. The clinical presentations of signs and symptoms are remarkably constant for most of the species of Trichinella, but in infections with Trichinella nativa and Trichinella britovi, classical symptoms of trichinellosis may be absent. It is important to be able to correlate the clinical presentation of trichinellosis with the life cycle of these helminths in order to make an accurate diagnosis. Knowledge of the epidemiology of the disease enables the physician to identify other potential cases, since most epidemics can be traced back to a common source of raw or undercooked meat. A comprehensive summary relating the most important clinical variables is presented graphically for easy reference to the text. Symptoms and signs are considered in relation to severity of infection. Laboratory findings and diagnostic techniques, including new modalities (e.g., DNA and antigen detection), are discussed. A discussion of treatment and preventive measures concludes our review.
Asunto(s)
Triquinelosis/diagnóstico , Triquinelosis/epidemiología , Animales , Reservorios de Enfermedades , Contaminación de Alimentos , Factores de Tiempo , Trichinella spiralis/crecimiento & desarrollo , Triquinelosis/prevención & control , Triquinelosis/psicología , Triquinelosis/transmisiónRESUMEN
From the end of 1991 to June, 1993, an epidemic neuropathy affecting 50,963 persons occurred in Cuba. Two clinical forms of the disease were observed: the optic form (with or without peripheral manifestations, 52% of the cases) and the peripheral form (48%). The epidemiological studies revealed nutritional disorders, mainly a vitamin B complex deficiency due to economic difficulties faced by the country in the "special period". Smoking habit and alcohol consumption were identified as risk factors, particularly for the most severe forms of the disease. In the virological studies of the cerebrospinal fluid (CSF) a Coxsackie 9 virus was isolated in Vero cells in 4% of the cases, as well as another agent with mild cytopathogenic effect in 80% of the samples. Sixty seven percent of the CSF samples inoculated to lactating mice caused the disease or the death of the animals. The viral persistence was confirmed in the cerebrospinal fluid during al least 21 days in a group of patients, and for a year in another group.
Asunto(s)
Neuritis Óptica/virología , Enfermedades del Sistema Nervioso Periférico/virología , Animales , Animales Recién Nacidos , Líquido Cefalorraquídeo/virología , Chlorocebus aethiops , Cuba/epidemiología , Enterovirus/aislamiento & purificación , Humanos , Ratones , Ratones Endogámicos BALB C , Neuritis Óptica/líquido cefalorraquídeo , Neuritis Óptica/epidemiología , Enfermedades del Sistema Nervioso Periférico/líquido cefalorraquídeo , Enfermedades del Sistema Nervioso Periférico/epidemiología , Células Vero , Cultivo de VirusRESUMEN
Results which allow to state the existence of antigenic relationships between viruses isolated from the cerebrospinal fluid of patients presenting with epidemic neuropathy and structures of the human central nervous system are reported. These evidences were obtained by 2 different and independent ways: 1) by the double diffusion method in agarose, immunoblot analysis, and immunohistochemical analysis it was confirmed that antibodies induced by isolated viruses react with antigens of the central and peripheral nervous system, 2) serum obtained by the immunization of a rabbit with human brain extract neutralizes the same viruses as those neutralized by hyperimmune sera obtained by isolations. The possible role of viruses as mediators of an autoimmune process in the pathogenesis of the disease is discussed.
Asunto(s)
Antígenos Virales/análisis , Antígenos/análisis , Encéfalo/inmunología , Enterovirus Humano B/inmunología , Enterovirus/inmunología , Neuritis Óptica/inmunología , Enfermedades del Sistema Nervioso Periférico/inmunología , Animales , Biopsia , Western Blotting , Cuba/epidemiología , Humanos , Sueros Inmunes/aislamiento & purificación , Inmunohistoquímica , Neuritis Óptica/epidemiología , Neuritis Óptica/etiología , Enfermedades del Sistema Nervioso Periférico/epidemiología , Enfermedades del Sistema Nervioso Periférico/etiología , Conejos , Nervio Sural/inmunología , Nervio Sural/metabolismo , Nervio Sural/patologíaRESUMEN
A study was made on 105 patients with Buruli ulcer in the Amansie West district, Ashanti Region, Ghana, representing 37.6% of the registered patients. The Tontokrom neighborhood showed the highest prevalence: 84 x 1,000 inhabitants. Predominance of females (54%) was observed. 74.8% did agricultural work and only 3 reported a previous trauma, predominantly with one lesion. Household contacts were identified. Coverage with the BCG vaccine was low. It is concluded that there has been a real increase of the prevalence of Buruli in the region during the last years, especially among children and women. This has become a serious problem due to its invalidating and irreversible sequelae. Measures of control are proposed.
Asunto(s)
Úlcera Cutánea/epidemiología , Adolescente , Adulto , Distribución por Edad , Anciano , Anciano de 80 o más Años , Niño , Preescolar , Femenino , Ghana/epidemiología , Humanos , Masculino , Persona de Mediana Edad , Prevalencia , Distribución por Sexo , Úlcera Cutánea/patologíaRESUMEN
Infants rats, a well known model for the experimental reproduction of bacterial meningitis, were used by us to test the protective potential of antibodies developed in humans who had been vaccinated with the Cuban antimeningitis vaccine (VA-MENGOCBC). Newborn rats were inoculated by the intraperitoneal and intranasal routes with suspensions of Neisseria meningitidis group B bacteria. Bacteremia kinetics were evaluated from blood and brain-spinal fluid cultures. Samples of the central nervous system were taken and smears of backbone fluids prepared for histopathologic evaluations. Characterization of bacteremia evolution, as well as the mean lethal dose of germs and histopathologic features, were determined. After standardization of the model, therapeutic schemes were applied using passive immunization pre- and post-infection with N. meningitidis. A significant level of protection was obtained in relation to control animals that received the same challenge doses.
Asunto(s)
Anticuerpos Antibacterianos/uso terapéutico , Inmunoglobulina G/uso terapéutico , Meningitis Meningocócica/terapia , Neisseria meningitidis/inmunología , Animales , Bacteriemia , Líquido Cefalorraquídeo/citología , Líquido Cefalorraquídeo/microbiología , Modelos Animales de Enfermedad , Femenino , Compuestos Férricos/farmacología , Humanos , Masculino , Meningitis Meningocócica/inmunología , Neisseria meningitidis/aislamiento & purificación , Neisseria meningitidis/patogenicidad , Ratas , Ratas Sprague-Dawley , Virulencia/efectos de los fármacosRESUMEN
The experimental model of graft vs. host disease (GvHD) has a potential use in the evaluation of different manipulation procedures of the immune system applicable to development of vaccines. In the present study an experimental model of GvHD in F1 (CBA/J x C57BL/6) mice by means of the parenteral inoculation of spleen lymphoid cells from parental male CBA/J to 10-day-old animals (experimental group) was developed. Animals inoculated with Medium 199 (n = 42) (Medium 199 group), or with splenic lymphoid cells either from the hybrids (n = 16) (F1 group), or from mice of the inbred strain Balb/c (n = 10) (Balb/c group) were used as controls. In all groups body and spleen weights, relative spleen index (RSI), and spleen index (SI) were determined. Additionally, histopathologic and morphometric studies were done in the spleens of the animals studied. Significant increases in body and spleen weights, RSI, and lymphocytic perimeter and area were associated with distinctive splenic GvHD lesions found in the experimental group. The experimental SI value was higher than twice the SI value of any of the control groups. We conclude that ours is a useful model of GvHD with many potential applications in the field of vaccine production.
Asunto(s)
Modelos Animales de Enfermedad , Enfermedad Injerto contra Huésped/inmunología , Animales , Peso Corporal , Femenino , Tolerancia Inmunológica , Inmunocompetencia , Linfocitos/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Tamaño de los Órganos , Bazo/anatomía & histología , Bazo/inmunologíaRESUMEN
In order to determine the optimal day for the evaluation of an experimental model of GvHD in F1 mice and the histopathologic evolution of the lesions in different organs, we studied 10-day-old F1 (CBA/J x C57BL/6) mice inoculated with splenic lymphoid cells of the male parental CBA/J strain (n = 42) that were sacrificed between 1 and 14 days postinoculation. The evolution of the relative spleen index (RSI) and the histopathologic lesions in different organs were also determined. F1 mice inoculated with Medium 199 were used as controls. Significant RSI increases (p < 0.0001) were found in the experimental group between 2 and 14 days postinoculation, with a peak at the eighth day, associated with the most severe histopathologic lesions in the organs studied. We suggest the eighth day as the optimal time for evaluation of this experimental model.