RESUMEN

Inorganic arsenic (iAs) is a carcinogen. Vegetables such as water spinach (Ipomoea aquatica Forssk.) and amaranth (Amaranthus mangostanus L.) are recognized as high-risk sources of iAs exposure because they can accumulate significant amounts of iAs and are widely consumed. To ensure safe cultivation conditions, this study aimed to establish prediction models for iAs concentration in the edible parts of water spinach and amaranth based on soil properties. Subsequently, health risk assessments associated with iAs exposure through the consumption of these vegetables were conducted using prediction models. Soil samples were collected from agricultural fields in Taiwan and used in the pot experiments. Pearson correlation and partial correlation analyses were used to explore the relationship between soil properties, including total As, clay, organic matter, iron oxides and available phosphates, and iAs concentration in edible parts of water spinach and amaranth. Prediction models based on soil properties were developed by stepwise multiple linear regression. Health risk assessments were conducted using the Monte Carlo algorithm. The results indicate that total As and organic matter contents in soil were major predictors of iAs concentration in water spinach, whereas those in amaranth were total As and clay contents. Therefore, higher health risks for consuming water spinach and amaranth are associated with higher levels of organic matter and clay contents in soil, respectively, and these are crucial factors to consider to ensure food safety. This study suggested that As-elevated soils enriched with organic matter and clay contents should be avoided when growing water spinach and amaranth, respectively.

Asunto(s)

Amaranthus , Arsénico , Contaminantes del Suelo , Verduras , Suelo , Arsénico/toxicidad , Arsénico/análisis , Arcilla , Inocuidad de los Alimentos , Contaminantes del Suelo/toxicidad , Contaminantes del Suelo/análisis

RESUMEN

In this paper, a cascaded microsphere compound lens (CMCL) is introduced, in which a 20-µm-diameter barium titanate glass (BTG) primary microsphere and a 250-nm-diameter or 200-nm-diameter polystyrene (PS) secondary microsphere array constitute CMCL1 and CMCL2, respectively. The field of view (FOV) depends on the size of the BTG microsphere, while the waist of the photon nanojet (PNJ) can be adjusted by the size of the PS microsphere. The narrower the waist of the PNJ, the higher the imaging resolution. In the experiment, a 200-nm-diameter hexagonally close-packed PS nanoparticle array is successfully observed by the CMCL with a high magnification of  ∼11.6× and a FOV of  ∼14µm, while the single BTG microsphere is incapable of observing the array. The point spread function is used to quantify the resolution of the CMCL. A well-designed CMCL can improve the imaging performances of a microsphere-assisted microscope.

RESUMEN

Here we find that a fully immersed low refractive index SiO2 microsphere (or a microcylinder, a yeast cell) can clearly distinguish a sample with sub-diffraction features in dark-field illumination mode. The resolvable area of the sample by microsphere-assisted microscopy (MAM) is composed of two regions. One region locates below the microsphere, and a virtual image of this part of the sample is formed by the microsphere first and then the virtual image is received by the microscope. The other region is around the edge of the microsphere, and this part of the sample is directly imaged by the microscope. The simulated region of the enhanced electric field on the sample surface formed by the microsphere is consistent with the resolvable region in the experiment. Our studies show that the enhanced electric field on the sample surface generated by the fully immersed microsphere plays an important role in dark-field MAM imaging, and this finding will have a positive effect on exploring novel mechanisms in resolution improvement of MAM.

RESUMEN

We present for the first time a surface plasmon-enhanced dark-field microsphere-assisted microscopy in imaging both low-contrast dielectric objects and metallic ones. We demonstrate, using an Al patch array as the substrate, the resolution and contrast in imaging low-contrast dielectric objects are improved compared to that of the metal plate substrate and a glass slide in dark-field microscopy (DFM). 365-nm-diameter hexagonally arranged SiO nanodots assembled on the three substrates can be resolved, with the contrast varied from 0.23 to 0.96, and the 300-nm-diameter hexagonally close-packed polystyrene nanoparticles can only be discerned on the Al patch array substrate. The resolution can be further improved by using the dark-field microsphere-assisted microscopy, and an Al nanodot array with a nanodot diameter of ∼65 nm and a center-to-center spacing of 125 nm can be just resolved, which cannot be distinguished in a conventional DFM. The focusing effect of the microsphere, as well as the excitation of the surface plasmons, provides evanescent illumination with enhanced local electric field (E-field) on an object. The enhanced local E-field acts as a near-field excitation source to enhance the scattering of the object, resulting in the improvement of imaging resolution.

RESUMEN

We fabricate both triangularly and circularly shaped Au, Ag, and Cr nanoparticle arrays and observe the imaging properties of these plasmonic nanostructures by BaTiO3 glass (BTG) microsphere-assisted microscopy. We experimentally find that the resolution of triangularly shaped Ag nanoparticle arrays is higher than that of Au and Cr ones, and a gap resolution of ∼λ/7.7 is demonstrated for the circularly shaped Au, Ag, and Cr nanostructures. Numerical simulations show that when a fully immersed BTG microsphere is dispersed on the surface of a plasmonic nanostructure sample, an enhanced electric field is generated in the vicinity of the sample, especially at the gap of the microsphere and the sample, due to the focusing effect of the microsphere and the excitation of localized surface plasmon resonance in the plasmonic nanostructure. The enhanced electric field in Ag nanostructures is significantly stronger than that in Au and Cr ones. Besides, the microsphere collects, amplifies, and propagates the enhanced near-field information to the far field, resulting in the improvement of imaging resolution.

RESUMEN

The purpose of the study was to assess the artificial saliva (AS) pH on ruminal fermentation and rumen bacteria community in the rumen simulation technique (RUSITEC) system. The experiment was performed in two treatments (low AS pH vs. high AS pH) with four replicates. The low AS pH was sustained by altering the composition of the AS (NaHCO3 from 9.8 to 1.96 g/L, Na2HPO4 from 9.3 to 1.86 g/L) according to McDougall's method. The diets were supplemented with 16 g basic diets with forage to the concentrate ratio of 50:50. The experiments were conducted over 13-day incubation periods, with 9 days adaption and 4 days sample collection. The results showed low AS pH decreased dry matter (DM) degradability (64.37 vs. 58.67%), organic matter (OM) degradability (64.38 vs. 59.32%), neutral detergent fiber (NDF) degradability (46.87 vs. 39.94%), acid detergent fiber (ADF) degradability (38.16 vs. 31.13%), and crude protein (CP) degradability (70.33 vs. 62.99%), respectively. Compared with the high AS pH, the low AS pH increased the proportion of butyrate (P = 0.008) and decreased the proportion of propionate (P < 0.001). At the bacteria community, the low AS pH increased the abundances of Spirochaetes (P = 0.001) and Synergistetes (P = 0.004) and decreased the Verrucomicrobia abundance (P = 0.004) in solid-associated bacteria. At the genus level, the low AS pH increased the abundance of Lactobacillus (P = 0.050) and decreased the abundance of Schwartzia (P = 0.002) in solid-associated bacteria. The abundances of Prevotellaceae_YAB2003_group (P = 0.040), Schwartzia (P = 0.002), and Ruminobacter (P = 0.043) were lower in the low AS pH group compared with the high AS pH group in liquid-associated bacteria. Low AS pH decreased the number of Ruminococcus albus, Ruminococcus flavefaciens, Fibrobacter succinogenes (P < 0.001) both in the solid- and liquid-associated bacteria, respectively. The results of the present study included three groups of bacteria communities according to the different sensitives to rumen pH: the abundances of Lactobacillus, Succinivibrio, and Prevotella_7 are increased with decreasing AS pH; the amounts of R. albus, R. flavefaciens, F. succinogenes as well as the abundances of Schwartzia and Ruminobacter decreased with the reducing AS pH; the abundances of Selenomonas_1, Rikenellaceae_RC9_gut_group, and Succiniclasticum were not affected by the AS pH in RUSTITEC.

RESUMEN

This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult Hu sheep (48.8 ± 0.23 kg, 0.5 years) were fed at 0700 and 1,800 with ad libitum access to water. The rumen fistula was installed in the same batch from 0600 to 0900. Monitoring the dry mater intake and the output of dry mater faces 1, 2, 4, 6, 8, 10, 12, 14 days after fistulated surgery. The collection of rumen fluid was arranged at 1d during rumen surgery (DRS1), 3d after rumen surgery (ARS3), and 14d after rumen surgery (ARS14) for volatile fatty acid (VFA) and DNA extraction for sequencing and bioinformatics analysis. There was no difference in DMI, the pH apparent digestibility of dry matter, organic matter, neutral detergent fiber, acid detergent fiber both before and 14 days after surgery. Increases were observed in the acetate and total VFA at ARS3. There was no difference in digestion of dry material, organic material, neutral detergent fiber, and acid detergent fiber before and after surgery. The relative abundance of Bacteroides decreased from 61.96% at DRS1 to 28.85% at ARS3. In comparison with the DRS1 and ARS3, the relative abundance of Firmicutes in the ARS14 increased to 44.58% (P < 0.01). Proteobacteria increased from 11.33% at DRS1 to 51.66% at ARS3 and then decreased to 11.39% at ARS14. Prevotella decreased form 61.06% at DRS1 to 28.04% in the ARS3. Succinivibrio increased from 8.32% at DRS1 to 48.58% at ARS3, but decreased to 10.43% in the ARS14. Compared with DRS1 and ARS3, the ARS14 was higher in the Simpson and Shannon index. As for the BugBase function prediction, rumen fistula surgery increased the microorganism abundance of aerobic and facultative anaerobic phenotype, and anaerobic phenotype was decreased in the ARS3. There was higher microorganism abundance of aerobic phenotype in the ARS14 than before fistula installation. In conclusion, the rumen fistula surgery destroys the anaerobic environment of rumen, leading to differences in rumen microbial diversity and function, but the apparent digestibility and total VFA were not affected.

RESUMEN

Objective: To investigate the results of in vitro fertilization among polycystic ovary syndrome (PCOS) patients using the long-acting long protocol regarding the relationship between menstrual patterns and adverse pregnancy outcomes. Design: Retrospective cohort study. Setting: University-affiliated reproductive medical center. Background: The menstrual patterns of patients with PCOS is considered related to metabolism; however, no study has analyzed the outcome of in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) in patients with PCOS who have different menstrual patterns. This study aimed to observe the outcomes of IVF/ICSI in patients with PCOS with different menstrual patterns who used the long-acting long protocol. Methods: This was a retrospective analysis in the first cycle of IVF/ICSI at the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from January 2017 to December 2019. In total, 1834 patients with PCOS were classified into the regular menstruation group (n=214), the oligomenorrhea group (n=1402), and the amenorrhea group (n=218). Results: PCOS patients who used the long-acting long protocol of IVF/ICSI had similar clinical pregnancy rates and live birth rates despite having different menstrual patterns. The overall incidence of adverse pregnancy outcomes, including abortion, spontaneous preterm birth (sPTB), gestational diabetes(GDM), hypertensive disorder inpregnancy (HDP), and premature rupture of membranes(PROM, was significantly higher in the amenorrhea group than in the regular menstrual and oligomenorrhea groups (25.88% vs. 30.41% vs. 43.69%; P = 0.013). Additionally, the rates of GDM (2.35% vs. 6.10% vs. 13.79%; P=0.015) and macrosomia (5.26% vs. 10.94% vs. 18.39%; P=0.026) in the amenorrhea group were significantly higher than those in the other two groups. Correction for confounding factors showed that menstrual patterns are related to the occurrence of adverse pregnancy outcomes. Amenorrhea is an independent risk factor for adverse pregnancy outcome (OR [odds ratio]: 2.039, 95% CI [confidence interval]: 1.087-3.822), GDM (OR: 5.023, 95% CI: 1.083-23.289), and macrosomia (OR: 4.918, 95% CI: 1.516-15.954). Conclusions: IVF/ICSI can achieve similar pregnancy and live birth rates in PCOS patients with different menstrual patterns. However, the overall incidence of adverse pregnancy outcomes in PCOS patients with amenorrhea is higher than that in patients with regular menstruation or oligomenorrhea.

Asunto(s)

Menstruación/fisiología , Síndrome del Ovario Poliquístico/fisiopatología , Resultado del Embarazo , Adulto , Femenino , Humanos , Embarazo , Índice de Embarazo , Nacimiento Prematuro , Estudios Retrospectivos , Adulto Joven

RESUMEN

Photoperiod-regulated floral transition is vital to the flowering plant. Luculia gratissima "Xiangfei" is a flowering ornamental plant with high development potential economically and is a short-day woody perennial. However, the genetic regulation of short-day-induced floral transition in L. gratissima is unclear. To systematically research the responses of L. gratissima during this process, dynamic changes in morphology, physiology, and transcript levels were observed and identified in different developmental stages of long-day- and short-day-treated L. gratissima plants. We found that floral transition in L. gratissima occurred 10 d after short-day induction, but flower bud differentiation did not occur at any stage under long-day conditions. A total of 1,226 differentially expressed genes were identified, of which 146 genes were associated with flowering pathways of sugar, phytohormones, photoperiod, ambient temperature, and aging signals, as well as floral integrator and meristem identity genes. The trehalose-6-phosphate signal positively modulated floral transition by interacting with SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 4 (SPL4) in the aging pathway. Endogenous gibberellin, abscisic acid, cytokinin, and jasmonic acid promoted floral transition, whereas strigolactone inhibited it. In the photoperiod pathway, FD, CONSTANS-LIKE 12, and nuclear factors Y positively controlled floral transition, whereas PSEUDO-RESPONSE REGULATOR 7, FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1, and LUX negatively regulated it. SPL4 and pEARLI1 positively affected floral transition. Suppressor of Overexpression of Constans 1 and AGAMOUSLIKE24 integrated multiple flowering signals to modulate the expression of FRUITFULL/AGL8, AP1, LEAFY, SEPALLATAs, SHORT VEGETATIVE PHASE, and TERMINAL FLOWER 1, thereby regulating floral transition. Finally, we propose a regulatory network model for short-day-induced floral transition in L. gratissima. This study improves our understanding of flowering time regulation in L. gratissima and provides knowledge for its production and commercialization.

RESUMEN

Background: Subchorionic hematoma (SCH) is common in early pregnancy achieved by in vitro fertilization-embryo transfer/frozen embryo transfer (IVF-ET/FET), and is associated with adverse obstetric outcomes. However, there are no methods known to accurately predict the occurrence of SCH. Objective: To establish a nomogram prediction model for predicting the risk of SCH in early pregnancy with IVF-ET/FET and to analyze pregnancy outcomes of patients with SCH. Methods: Patients who underwent IVF-ET/FET treatment and were diagnosed with clinical pregnancy were enrolled in our study. A total of 256 patients with SCH were enrolled in the SCH group, and 526 patients without SCH in the control group. Logistic regression was used to screen risk factors for SCH, and the nomogram was developed according to the regression coefficient of relevant variables. Discrimination, effect, calibration, and the predictive model's clinical usefulness were assessed using the C-index, the area under the receiver operating characteristic standard curve, calibration plot, and decision curve analysis. Internal validation was assessed using bootstrapping validation. The effects of SCH on pregnancy outcomes were analyzed. Results: A multivariate logistic regression analysis showed that fresh embryo transfer, polycystic ovary syndrome, hydrosalpinx, and thin endometrium were risk factors affecting the occurrence of SCH. Based on the above factors, a predictive model for the risk of SCH was created. The model displayed good discrimination, with a C-index of 0.783 (95% confidence interval: 0.750-0.816), area under the receiver operating characteristic standard curve of 0.783, and good calibration. A high C-index value of 0.765 could still be reached in the interval validation. Decision curve analysis showed that the nomogram was clinically useful when the intervention was decided at the SCH possibility threshold of 4%-87%. For patients with successful deliveries, the occurrence of SCH did not influence the gestational weeks of delivery, mode of delivery, preterm birth, height, and weight of the newborn. Conclusion: We screened the risk factors for SCH in patients who underwent IVF-ET/FET treatment. Successful establishment of a nomogram can effectively predict the occurrence of SCH. Furthermore, the incidence of miscarriage is higher in patients with SCH.

Asunto(s)

Transferencia de Embrión/métodos , Fertilización In Vitro/métodos , Hematoma/diagnóstico , Adulto , Área Bajo la Curva , Calibración , Criopreservación , Toma de Decisiones , Reacciones Falso Positivas , Femenino , Humanos , Análisis Multivariante , Nomogramas , Valor Predictivo de las Pruebas , Embarazo , Resultado del Embarazo , Probabilidad , Análisis de Regresión , Reproducibilidad de los Resultados , Riesgo , Factores de Riesgo

RESUMEN

Polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder in women of childbearing age. Recent studies suggest important roles for lncRNAs in PCOS development. Based on the hypothesis that lncRNAs are able to regulate mRNA functions by competitive binding to shared miRNAs, the present work sought to construct a PCOS-related lncRNA-mRNA network (PCLMN) to identify key lncRNAs with dysregulated expression and potential prognostic and therapeutic relevance. A global background network was constructed after retrieving lncRNA-miRNA and miRNA-mRNA pairs from the lncRNASNP2, miRTarBase and StarBase databases. Based on gene expression profiles from ovarian granulosa cells from PCOS patients and controls in the GEO's GSE95728 dataset, the PCLMN was then constructed by applying hypergeometric testing. Using topological analysis, we identified 3 lncRNAs (LINC00667, AC073172.1 and H19) ranking within the top-ten gene lists for all three centrality measures. We then explored their subcellular localization, performed functional module analyses, and identified 4 sex hormone-related transcription factors as potential regulators of their expression. Significant associations with inflammation, oxidative stress, and apoptosis-related processes and pathways were revealed for the key lncRNAs in our PCMLN. Further studies verifying the mRNA/lncRNA relationships identified herein are needed to clarify their clinical significance.

Asunto(s)

Regulación de la Expresión Génica/genética , Redes Reguladoras de Genes/genética , Síndrome del Ovario Poliquístico/genética , ARN Largo no Codificante/genética , ARN Mensajero/genética , Femenino , Humanos

RESUMEN

OBJECTIVE: To study the association between hyperandrogenism (HA) and adverse pregnancy outcomes in patients with different polycystic ovary syndrome phenotypes undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI). METHODS: We reviewed all eligible articles published up to October 2020 after searching in PubMed, Embase, Cochrane Library, Web of Science, Wanfang Data, and CNKI databases. The primary outcomes were the clinical pregnancy rate (CPR), miscarriage rate (MR), and live birth rate (LBR), whereas the secondary outcomes were the number of retrieved oocytes and endometrial thickness. Risk ratios (RRs) or mean differences with 95% confidence intervals (CIs) were calculated to estimate the HA impact on IVF/ICSI outcomes in patients with polycystic ovary syndrome (PCOS) phenotypes. RESULTS: Of the 789 trials identified, nine retrospective studies involving 3037 patients with PCOS were included. Compared to the PCOS group with normal androgen levels, the PCOS group with HA exhibited increased MR (RR: 1.56, 95% CI: 1.13, 2.16); the CPR (RR: 0.88, 95% CI: 0.77, 1.01) and LBR (RR: 0.79, 95% CI: 0.55, 1.11) were not significantly different between these groups. Subgroup analysis revealed that the CPR was lower in the polycystic ovarian (PCO)-morphology + HA + oligo-anovulation (AO) group than in the PCO + AO group (RR: 0.81, 95% CI: 0.67, 0.99). Among Asians, the PCOS/HA group had increased MR (RR: 1.56, 95% CI: 1.06, 2.31) and showed thinner endometrial thickness. However, among Caucasians, no differences were observed between the two groups. CONCLUSIONS: HA may have adverse effects on clinical pregnancy and miscarriage outcomes in different PCOS phenotypes, particularly among Asians.

Asunto(s)

Hiperandrogenismo/complicaciones , Síndrome del Ovario Poliquístico/complicaciones , Resultado del Embarazo/epidemiología , Inyecciones de Esperma Intracitoplasmáticas , Aborto Espontáneo/epidemiología , Pueblo Asiatico/estadística & datos numéricos , Endometrio/patología , Femenino , Humanos , Nacimiento Vivo/epidemiología , Recuperación del Oocito , Fenotipo , Síndrome del Ovario Poliquístico/patología , Embarazo , Índice de Embarazo

RESUMEN

Rhododendron meddianum is a critically endangered species with important ornamental value and is also a plant species with extremely small populations. In this study, we used double digest restriction-site-associated DNA sequencing (ddRAD) technology to assess the genetic diversity, genetic structure and demographic history of the three extant populations of R. meddianum. Analysis of SNPs indicated that R. meddianum populations have a high genetic diversity (π = 0.0772 ± 0.0024, H E  = 0.0742 ± 0.002). Both F ST values (0.1582-0.2388) and AMOVA showed a moderate genetic differentiation among the R. meddianum populations. Meanwhile, STRUCTURE, PCoA and NJ trees indicated that the R. meddianum samples were clustered into three distinct genetic groups. Using the stairway plot, we found that R. meddianum underwent a population bottleneck about 70,000 years ago. Furthermore, demographic models of R. meddianum and its relative, Rhododendron cyanocarpum, revealed that these species diverged about 3.05 (2.21-5.03) million years ago. This divergence may have been caused by environmental changes that occurred after the late Pliocene, e.g., the Asian winter monsoon intensified, leading to a drier climate. Based on these findings, we recommend that R. meddianum be conserved through in situ, ex situ approaches and that its seeds be collected for germplasm.

RESUMEN

Objectives: Lateral habenula (LHb) is a key brain structure for mediating behavioural responses to aversive stimuli. It receives presynaptic inputs from ventral pallidum (VP) which relates to reward, motivation and hedonics. This study investigated the role of glutamatergic VP-LH projection in negative emotions and depression-like behaviour.Methods: The glutamatergic VP-LHb circuits in Vglut2-Cre mice were activated or inhibited using optogenetic manipulation. Real time place aversion test was performed to access aversive behaviour. Mice underwent chronic social defeat stress or subthreshold social defeat stress paradigm. Then social interaction test and tail suspension test were carried out to evaluate the stress-induced depression-like behaviours.Results: Activation of VP-LHb glutamatergic projections induced aversive behaviour in the real time place aversion test. Activation of this circuit induced depressive-like phenotype after social stress, while optogenetic inhibition of this circuit exerted an antidepressant effect in social stress susceptible mice. Local LHb ketamine administration rescued the depression-like phenotype caused by activation of this circuit.Conclusions: Data from our study demonstrated an involvement of the glutamatergic VP-LHb circuit in the stress-induced depression-related behaviours.

Asunto(s)

Prosencéfalo Basal/fisiología , Depresión/fisiopatología , Habénula/fisiología , Vías Nerviosas , Transmisión Sináptica , Animales , Masculino , Ratones , Recompensa

RESUMEN

Purpose: To evaluate in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) outcome in women with genital tuberculosis (GTB).Methods: One hundred and fifty-five women with genital tuberculosis constituted the study group (Group A), consisting of 25 patients with endometrial tuberculosis (Group A1) and 130 patients with tubal tuberculosis (Group A2). Women with non-tuberculous tubal infertility were matched by age and study period served as controls (Group B).Results: Patients with GTB had significantly reduced endometrial thickness, high-quality embryos rate, implantation rate as compared with controls (p < .05), no differences were found in other pregnancy parameters. In addition, the endometrial thickness, fertilization rate, high-quality embryos rate and implantation rate were also significantly lower in women with endometrial tuberculosis as compared with controls. And the cumulative pregnancy rate in endometrial tuberculosis was significantly decreased compared with tubal tuberculosis and controls (p < .05). However, IVF/ICSI pregnancy outcomes in patients with tubal tuberculosis showed no difference as compared with controls (p > .05). Also, rates of miscarriage, preterm birth, obstetrical complications, and neonatal problems did not differ among three groups.Conclusions: In conclusion, IVF/ICSI-ET remains the most optimal method for the treatment of female infertility associated with tubal tuberculosis. However, patients with endometrium tuberculosis showed significantly reduced fertilization, implantation and cumulative pregnancy rates.

Asunto(s)

Fertilización In Vitro , Resultado del Embarazo/epidemiología , Tuberculosis de los Genitales Femeninos/epidemiología , Tuberculosis de los Genitales Femeninos/terapia , Adulto , Antituberculosos/uso terapéutico , Estudios de Casos y Controles , China/epidemiología , Endometritis/complicaciones , Endometritis/epidemiología , Endometritis/microbiología , Femenino , Humanos , Recién Nacido , Infertilidad Femenina/diagnóstico , Infertilidad Femenina/epidemiología , Infertilidad Femenina/terapia , Masculino , Embarazo , Índice de Embarazo , Nacimiento Prematuro/epidemiología , Nacimiento Prematuro/etiología , Salpingitis/complicaciones , Salpingitis/epidemiología , Salpingitis/microbiología , Inyecciones de Esperma Intracitoplasmáticas , Resultado del Tratamiento , Tuberculosis de los Genitales Femeninos/complicaciones , Tuberculosis de los Genitales Femeninos/diagnóstico , Adulto Joven

RESUMEN

Objective: To investigate the effect of seasons on the incidence of high risk of ovarian hyperstimulation syndrome (OHSS) after in oocyte retrieval in patients with polycystic ovarian syndrome (PCOS) and to establish a nomogram to predict the risk of OHSS. Design: Single-center, retrospective study. Setting: University-affiliated reproductive medicine center. Patients: A total of 2,030 infertility patients with PCOS underwent the follicular phase long-acting long protocol IVF/ICSI in the reproductive medicine center from January 2017 to December 2019. Interventions: None. Main outcome measures: Logistic regression analysis was used to analyze the factors associated with a high risk of OHSS. We established a nomogram to predict the risk of OHSS in infertility patients with PCOS after oocyte retrieval. Results: The incidence of patients at high risk of OHSS was significantly different from season-to-season and was especially higher in the summer and winter. Multivariate logistic analysis showed that gonadotropin dosage, number of retrieved oocytes, estradiol level, average bilateral ovarian diameter on the day human chorionic gonadotropin was administered, type of infertility, and average temperature were independent risk factors for OHSS after oocyte retrieval in PCOS patients. Based on the above independent risk factors, we constructed a prediction model for OHSS risk. To evaluate the efficiency of the prediction model, we calculated the C-index (0.849), area under the receiver operating characteristic curve (0.849), and internal validation C-index (0.846). Decision curve analysis suggested that the prediction model exhibited significant net benefits. Conclusions: The incidence of PCOS patients at high risk for OHSS after oocyte retrieval fluctuated with seasonal temperature changes, and was significantly higher in extreme climates. The prediction model had favorable predictive performance and clinical application value.

Asunto(s)

Recuperación del Oocito/efectos adversos , Recuperación del Oocito/tendencias , Síndrome de Hiperestimulación Ovárica/epidemiología , Síndrome del Ovario Poliquístico/epidemiología , Índice de Embarazo/tendencias , Estaciones del Año , Adulto , Transferencia de Embrión/métodos , Transferencia de Embrión/tendencias , Femenino , Humanos , Recién Nacido , Nomogramas , Síndrome de Hiperestimulación Ovárica/diagnóstico , Síndrome de Hiperestimulación Ovárica/terapia , Síndrome del Ovario Poliquístico/diagnóstico , Síndrome del Ovario Poliquístico/terapia , Embarazo , Estudios Retrospectivos , Factores de Riesgo

RESUMEN

In order to make a deep analysis of depression, the construction and recognition of functional brain network model based on depression is mainly studied. Firstly, the relevant information of the subjects is introduced in turn. The data of fMRI (functional magnetic resonance imaging) are pretreated, static and dynamic functional connections are analyzed, statistical analysis and testing are carried out, and then the results are analyzed and discussed. The results show that static functional connectivity provides a new research perspective and means for further understanding the connection patterns of brain functional networks in unipolar depression and bipolar depression. Dynamic functional connectivity analysis (DFA) extends the previous studies on unipolar depression and bipolar depression, and provides a potential biological marker for clinical identification of unipolar depression and bipolar depression.

Asunto(s)

Trastorno Bipolar , Encéfalo/diagnóstico por imagen , Modelos Biológicos , Red Nerviosa , Adulto , Femenino , Humanos , Imagen por Resonancia Magnética , Masculino , Adulto Joven

RESUMEN

Radiation-induced brain injury involves acute, early delayed and late delayed damage based on the time-course and clinical manifestations. The acute symptoms are mostly transient and reversible, whereas the late delayed radiation-induced changes are progressive and irreversible. Therefore, evaluation of the organ-specific early response to ionizing radiation exposure is necessary for improving treatment strategies and minimizing possible damage at an early stage after radiation exposure. In the current study, the gas chromatography-mass spectrometry technique based on metabolomics coupled with metabolic correlation network was applied to investigate the early metabolic characterization of rat brain tissues following irradiation. Our findings showed that the metabolic response to irradiation was not just limited to the variations of individual metabolite levels, but also accompanied by alterations of network correlations among various metabolites. Metabolite clustering indicated that energy metabolism disorder and inflammation response were induced following radiation exposure. The correlation networks revealed that the strong positive correlations of differential metabolites were highly reduced and significant negative linkages were highlighted in irradiated groups even without statistical changes in metabolic levels. Our findings provided new insights into our understanding of the radiation-induced acute brain injury mechanism and clues as to the therapy target for clinical applications.

Asunto(s)

Lesiones Encefálicas/metabolismo , Encéfalo/metabolismo , Cromatografía de Gases y Espectrometría de Masas/métodos , Metaboloma/efectos de la radiación , Traumatismos Experimentales por Radiación/metabolismo , Animales , Masculino , Redes y Vías Metabólicas/efectos de la radiación , Metabolómica , Ratas , Ratas Wistar , Irradiación Corporal Total/efectos adversos

RESUMEN

We experimentally demonstrate that pincushion distortion is obvious when using a BaTiO3 glass (BTG) microsphere fully immersed in ethanol or SU-8 2002 resist to image a Blu-ray disk with sub-diffraction features. The distortion is related to the layer thickness of the immersing medium. For a BTG microsphere partially immersed in the SU-8 resist where the SU-8 thickness is around 4/5 the diameter of the microsphere, its distortion decreases dramatically, but it can still clearly resolve the Blu-ray disk. For such a partially immersed microsphere, the calculated position of the photonic nanojet is outside the microsphere and close to the object, indicating the microsphere has a super-resolution imaging property, and the distortion simulated by ZEMAX is decreased.

RESUMEN

In this paper, the imaging properties of a cascaded microsphere lens are studied. The cascaded microsphere lens consists of two lenses. A hexagonally close-packed 960-nm-diameter array of polystyrene microspheres is used as the first lens. The second lens is a single silica microsphere with a diameter of about 5 µm. A Blu-ray disc is observed by both the cascaded microsphere lens and the single silica microsphere. Studies reveal that the magnification of the cascaded microsphere lens is about 1.4 times greater than that of the single silica microsphere, while the field of view of the cascaded microsphere lens, which is close to the diameter of the polystyrene microsphere, is decreased. The focal position of the cascaded lens microsphere must be close to the sample in order to observe it.