RESUMEN
Pseudomonas infections continue to be an important problem in the hospital environment. Serious infections are always invariably associated with severe underlying conditions or with diminished host resistance. The increasingly resistance of strains and hospital epidemics favour the organism prevalence. During 1978, Pseudomonas aeruginosa was isolated in 154 cultures from a variety of biological samples in the hospital. Tests of biochemical identification, serological typing, and typing by pyocine production were carried out. Susceptibility to aminoglycosides and beta-lactamic antibiotics was also tested. Serological study demonstrated a higher incidence of 4 and 11 serotypes; 69.4 percent corresponded to type I when typing by pyocine production was carried out. No relationship between serotypes and pyocine-types has been found. Ticarcillin showed a greater activity than carbenicillin (minimal inhibitory concentration less than or equal to 16 micrograms/ml). Amikacin, tobramicin, sisomicin and gentamicin inhibited 83.7 percent, 73.6 percent, 70.7 percent and 69.4 percent of the isolated strains, respectively.
Asunto(s)
Aminoglicósidos/farmacología , Antibacterianos/farmacología , Farmacorresistencia Microbiana , Pseudomonas aeruginosa/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , SerotipificaciónRESUMEN
The Limulus test was applied to determine bacterial endotoxemia in 42 patients suspected of having septicemia. Hemocultures were practiced simultaneously. A comparative study is made of the two presently most commonly recommended tests for neutralizing the factors inhibiting the endotoxin-amebocyte reaction. According to our results the dilution of plasma in isotonic saline solution is preferable; we obtained a sensitivity of determination of 0.0001 micrograms of E. coli endotoxin per ml of plasm. Seventeen cases of bacteriemia were diagnosed, only one of which with sepsis due to Serratia marcescens responded positively to the limulus test. The test was positive in three of the remaining 25 patients. Two of them had liver failure. The basic role that liver failure plays in the high level of endotoxin in the blood is discussed. The prognostic value of this test and the low correlation between bacteremia and endotoxemia are also commented on