RESUMEN
OBJECTIVE: To draw attention to complications that might arise in any Kawasaki disease (KD) stage, risk factors contributing to the onset of complications and possible transient or permanent disease sequelae. METHODS: Prospective study (clinical cohort) conducted between April 2002 and April 2009 of 115 patients with KD admitted to the Pediatric Rheumatology Clinic of the General Hospital of the Federal District, Brazil. All patients were sequentially assessed with clinical and laboratory examinations, Doppler echocardiography, imitanciometry, auditory evoked potentials, psychological evaluation, ophthalmologic examination and, in one patient with chorea, cerebral magnetic resonance angiography. In all patients, a questionnaire assessing the possible presence of cognitive, emotional, behavioral and social disorders was applied. RESULTS: Twenty-five patients (21.7%) had coronary aneurisms. Thirty eight patients (33%) had a sensorineural auditory loss during the acute and subacute phases of the disease and 13 patients (11.3%) maintained the auditory loss six months after the first assessment. Other complications observed were as follows: facial palsy in one patient (0.9%), ataxia in acute and subacute phases in 11 (9.5%); 15 patients had ophthalmologic complications (13.2%), with uveitis in 13, papilledema in one patient, and conjunctival hemorrhage in another patient. One patient experienced chorea (0.9%), with a magnetic resonance angiography showing changes consistent with cerebral ischemia. In one patient, a thoracic aorta aneurism was found (0.9%) and another patient had a necrotizing vasculitis progressing to peripheral gangrene and tongue tip loss (0.9%). Behavioral changes over convalescence were observed in 23 children. CONCLUSION: KD may progress with several complications even within months of the disease acute phase, eventually resulting in permanent sequelae. The earlier the diagnosis and therapeutic intervention with IV IgG administration are, the lower will be the occurrence of complications; the presence of thrombocytosis, anemia and elevated and extended inflammatory activity are risk factors for complication arising.
Asunto(s)
Síndrome Mucocutáneo Linfonodular/complicaciones , Aneurisma de la Aorta Torácica/etiología , Ataxia/etiología , Niño , Preescolar , Aneurisma Coronario/etiología , Parálisis Facial/etiología , Femenino , Pérdida Auditiva Sensorineural/etiología , Humanos , Lactante , Masculino , Trastornos Mentales/etiología , Estudios Prospectivos , Factores de RiesgoRESUMEN
OBJETIVO: Chamar a atenção para as complicações, que podem surgir em qualquer fase da Doença de Kawasaki (DK), para os fatores de risco que contribuem para o aparecimento dessas complicações e para as possíveis sequelas da doença, sejam elas transitórias ou permanentes. MÉTODOS: Estudo prospectivo (coorte clínica) realizado entre abril de 2002 e abril de 2009 de 115 pacientes com DK internados no serviço de Reumatologia Pediátrica do Hospital Geral do Distrito Federal. Todos os pacientes foram sequencialmente avaliados com exames clínicos e laboratoriais, ecocardiogramas com Doppler, imitanciometria, potenciais evocados auditivos, avaliação psicológica, exame oftalmológico e, em um paciente com coreia, angiorressonância magnética cerebral. Em todos os pacientes foram aplicados questionários avaliando a possível presença de dificuldades cognitivas, emocionais, comportamentais e sociais. RESULTADOS: Vinte e cinco pacientes (21,7 por cento) apresentaram aneurismas de coronárias. Trinta e oito pacientes (33 por cento) apresentaram perda auditiva neurossensorial durante a doença aguda e subaguda, e 13 pacientes (11,3 por cento) mantiveram a perda auditiva seis meses após a primeira avaliação. Outras complicações observadas foram: paralisia facial em um paciente (0,9 por cento), ataxia na fase aguda e subaguda em 11 pacientes (9,5 por cento), complicações oftalmológicas em 15 pacientes (13,2 por cento), constatando-se uveíte em 13, edema de papila em um paciente e hemorragia conjuntival em outro. Um paciente apresentou coreia (0,9 por cento) sendo que a angioressonância magnética evidenciou alterações compatíveis com isquemia cerebral. Em um paciente constatou-se a presença de aneurisma de aorta torácica (0,9 por cento), e outro apresentou vasculite necrosante que evoluiu com gangrena periférica e perda da ponta da língua (0,9 por cento). Alterações de comportamento durante a convalescença (20 por cento) foi observada em 23 crianças. CONCLUSÃO: A DK pode evoluir com complicações diversas, mesmo meses após a fase aguda da doença, eventualmente resultando em sequelas permanentes. Quanto mais precoce forem o diagnóstico e a intervenção terapêutica com a administração de IgG IV, menor será a ocorrência de complicações. Presença de trombocitose, anemia e de atividade inflamatória elevada e por tempo prolongado são fatores de risco para o aparecimento de complicações.
OBJECTIVE: To draw attention to complications that might arise in any Kawasaki disease (KD) stage, risk factors contributing to the onset of complications and possible transient or permanent disease sequelae. METHODS: Prospective study (clinical cohort) conducted between April 2002 and April 2009 of 115 patients with KD admitted to the Pediatric Rheumatology Clinic of the General Hospital of the Federal District, Brazil. All patients were sequentially assessed with clinical and laboratory examinations, Doppler echocardiography, imitanciometry, auditory evoked potentials, psychological evaluation, ophthalmologic examination and, in one patient with chorea, cerebral magnetic resonance angiography. In all patients, a questionnaire assessing the possible presence of cognitive, emotional, behavioral and social disorders was applied. RESULTS: Twenty-five patients (21.7 percent) had coronary aneurisms. Thirty eight patients (33 percent) had a sensorineural auditory loss during the acute and subacute phases of the disease and 13 patients (11.3 percent) maintained the auditory loss six months after the first assessment. Other complications observed were as follows: facial palsy in one patient (0.9 percent), ataxia in acute and subacute phases in 11 (9.5 percent); 15 patients had ophthalmologic complications (13.2 percent), with uveitis in 13, papilledema in one patient, and conjunctival hemorrhage in another patient. One patient experienced chorea (0.9 percent), with a magnetic resonance angiography showing changes consistent with cerebral ischemia. In one patient, a thoracic aorta aneurism was found (0.9 percent) and another patient had a necrotizing vasculitis progressing to peripheral gangrene and tongue tip loss (0.9 percent). Behavioral changes over convalescence were observed in 23 children. CONCLUSION: KD may progress with several complications even within months of the disease acute phase, eventually resulting in permanent sequelae. The earlier the diagnosis and therapeutic intervention with IV IgG administration are, the lower will be the occurrence of complications; the presence of thrombocytosis, anemia and elevated and extended inflammatory activity are risk factors for complication arising.
Asunto(s)
Niño , Preescolar , Femenino , Humanos , Lactante , Masculino , Síndrome Mucocutáneo Linfonodular/complicaciones , Aneurisma de la Aorta Torácica/etiología , Ataxia/etiología , Aneurisma Coronario/etiología , Parálisis Facial/etiología , Pérdida Auditiva Sensorineural/etiología , Trastornos Mentales/etiología , Estudios Prospectivos , Factores de RiesgoRESUMEN
El presente trabajo responde esencialmente a la necesidad de capacitar a los recursos humanos que intervienen en el proceso de formación de los profesionales de la salud (docentes, trabajadores no docentes y directivos) para asumir con plenitud la tarea del desarrollo del trabajo formativo sobre las actuales concepciones filosóficas, pedagógicas y psicológicas, que están elaboradas tanto a escala nacional como internacional. Este diplomado contribuye al desarrollo sobre bases científicas de la estrategia educativa y los proyectos educativos que se elaboran en el Centro. Por ello el objetivo es el de hacer una propuesta de actividad de postgrado (Diplomado) que permita la preparación teórica y metodológica del personal docente y de dirección de la Educación Médica Superior para desarrollar la educación en valores a través del proyecto educativo, así como brindar herramientas prácticas para el diseño de las estrategias educativas. El diplomado que se muestra está aprobado como actividad de los programas nacionales e internacionales de postgrados del MINSAP y la ENSAP, y ha sido impartido en dos versiones en el ISCM Carlos J Finlay de Camaguey con resultados positivos. Este curso de superación es el resultado lógico del desarrollo de tesis de maestrías y de otros cursos cortos contemplados en el plan de superación del Instituto de varios profesores involucrados en el mismo (AU)
This work responds essentially to the necessity of qualifying the human resources that intervene in the health professionals' formation process to assume with great quality the carrying out of the formative work, taking into consideration the current philosophical, pedagogical and psychological conceptions elaborated at national as well as international levels. This postgraduate course contributes to the development on scientific bases of the educational strategy and the educational projects that are elaborated. For this reason, the main objective is making a proposal of postgraduate studies that foster professors ' theoretical and methodological preparation, to develop values education through the educational project, as well as to offer practical tools for the design of educational strategies. The postgraduate course that is presented is approved as an activity of the national and international programs of postgraduate studies of MINSAP and ENSAP, and it has been carried out in two versions in Carlos J Finlay Medical School in Camagüey with positive results. This course is the logical result of the development of master degree scientific papers and of other short courses contemplated in the Medical School professors' development planning (AU)
Asunto(s)
Humanos , Educación Médica , Valores SocialesRESUMEN
AIMS: To determine the ability of probiotic lactobacilli to improve the treatment of vulvovaginal candidiasis (VVC) using a randomized, double-blind and placebo-controlled trial. METHODS AND RESULTS: Fifty-five women diagnosed with VVC by vaginal discharge positive for Candida spp. (according to culture method) associated with at least one of the symptoms (itching and burning vaginal feeling, dyspareunia and dysuria), were treated with single dose of fluconazole (150 mg) supplemented every morning for the following 4 weeks with two placebo or two probiotic capsules (containing Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14). At 4 weeks, the probiotic treated group showed significantly less vaginal discharge associated with any of the above mentioned symptoms (10.3%vs 34.6%; P = 0.03) and lower presence of yeast detected by culture (10.3%vs 38.5%; P = 0.014). CONCLUSION: This study has shown that probiotic lactobacilli can increase the effectiveness of an anti-fungal pharmaceutical agent in curing disease. SIGNIFICANCE AND IMPACT OF THE STUDY: This novel finding of probiotic lactobacilli augmenting the cure rate of yeast vaginitis, not only offers an alternative approach to a highly prevalent condition that adversely affects the quality of life of women around the world, but also raises the question of how this combination works.
Asunto(s)
Antifúngicos , Candida/efectos de los fármacos , Candidiasis Vulvovaginal , Fluconazol , Lacticaseibacillus rhamnosus , Limosilactobacillus reuteri , Probióticos , Adolescente , Adulto , Antifúngicos/administración & dosificación , Antifúngicos/uso terapéutico , Candida/clasificación , Candida/aislamiento & purificación , Candidiasis Vulvovaginal/tratamiento farmacológico , Candidiasis Vulvovaginal/microbiología , Candidiasis Vulvovaginal/terapia , Método Doble Ciego , Femenino , Fluconazol/administración & dosificación , Fluconazol/uso terapéutico , Humanos , Persona de Mediana Edad , Probióticos/administración & dosificación , Probióticos/uso terapéutico , Resultado del Tratamiento , Adulto JovenRESUMEN
El presente trabajo responde esencialmente a la necesidad de capacitar a los recursos humanos que intervienen en el proceso de formación de los profesionales de la salud (docentes, trabajadores no docentes y directivos) para asumir con plenitud la tarea del desarrollo del trabajo formativo sobre las actuales concepciones filosóficas, pedagógicas y psicológicas, que están elaboradas tanto a nivel nacional como internacional. Este diplomado contribuye al desarrollo sobre bases científicas de la estrategia educativa y los proyectos educativos que se elaboran en el Centro. Por ello el objetivo es el de hacer una propuesta de actividad de postgrado (Diplomado) que permita la preparación teórica y metodológica del personal docente y de dirección de la Educación Médica Superior para desarrollar la educación en valores a través del proyecto educativo, así como brindar herramientas prácticas para el diseño de las estrategias educativas. El diplomado que se muestra está aprobado como actividad de los programas nacionales e internacionales de postgrados del MINSAP y la ENSAP, y ha sido impartido en dos versiones en el ISCM Carlos J Finlay de Camaguey con resultados positivos. Este curso de superación es el resultado lógico del desarrollo de tesis de maestrías y de otros cursos cortos contemplados en el plan de superación del Instituto de varios profesores involucrados en el mismo(AU)
Asunto(s)
Educación de Postgrado , Estrategias de Salud , DocentesRESUMEN
El presente trabajo responde esencialmente a la necesidad de capacitar a los recursos humanos que intervienen en el proceso de formación de los profesionales de la salud (docentes, trabajadores no docentes y directivos) para asumir con plenitud la tarea del desarrollo del trabajo formativo sobre las actuales concepciones filosóficas, pedagógicas y psicológicas, que están elaboradas tanto a escala nacional como internacional. Este diplomado contribuye al desarrollo sobre bases científicas de la estrategia educativa y los proyectos educativos que se elaboran en el Centro. Por ello el objetivo es el de hacer una propuesta de actividad de postgrado (Diplomado) que permita la preparación teórica y metodológica del personal docente y de dirección de la Educación Médica Superior para desarrollar la educación en valores a través del proyecto educativo, así como brindar herramientas prácticas para el diseño de las estrategias educativas. El diplomado que se muestra está aprobado como actividad de los programas nacionales e internacionales de postgrados del MINSAP y la ENSAP, y ha sido impartido en dos versiones en el ISCM Carlos J Finlay de Camaguey con resultados positivos. Este curso de superación es el resultado lógico del desarrollo de tesis de maestrías y de otros cursos cortos contemplados en el plan de superación del Instituto de varios profesores involucrados en el mismo.
This work responds essentially to the necessity of qualifying the human resources that intervene in the health professionals formation process to assume with great quality the carrying out of the formative work, taking into consideration the current philosophical, pedagogical and psychological conceptions elaborated at national as well as international levels. This postgraduate course contributes to the development on scientific bases of the educational strategy and the educational projects that are elaborated. For this reason, the main objective is making a proposal of postgraduate studies that foster professors theoretical and methodological preparation, to develop values education through the educational project, as well as to offer practical tools for the design of educational strategies. The postgraduate course that is presented is approved as an activity of the national and international programs of postgraduate studies of MINSAP and ENSAP, and it has been carried out in two versions in Carlos J Finlay Medical School in Camagüey with positive results. This course is the logical result of the development of master degree scientific papers and of other short courses contemplated in the Medical School professors development planning.
RESUMEN
Rice blast is the most important fungal disease of rice and is caused by Pyricularia oryzae Sacc. (Telomorph Magnoporthe grisea Barr.). Seven randomly amplified polymorphic DNA (RAPD) markers OPA5, OPG17, OPG18, OPG19, OPF9, OPF17 and OPF19 showed very clear polymorphism in resistant cultivar lines which differed from susceptible lines. By comparing different susceptible lines, nine DNA amplifications of seven primers (OPA5(1000), OPA5(1200,) OPG17(700), OPG18(850), OPG19(500), OPG19(600), OPF9(600), OPF17(1200) and OPF19(600)) were identified as dominant markers for the blast resistant gene in resistant cultivar lines. These loci facilitate the indirect scoring of blast resistant and blast susceptible genotypes. The codomine RAPDs markers will facilitate marker-assisted selection of the blast resistant gene in two blast resistant genotypes of rice (Labelle and Line 11) and will be useful in rice breeding programs.
Asunto(s)
Oryza/genética , Polimorfismo Genético , Brasil , Marcadores GenéticosRESUMEN
Over the last two decades, mutational techniques have become one of the most important tools available to progressive rice- breeding programs. In a mutation-breeding program initiated in 1999 at the Instituto Agronômico of Campinas, SP, Brazil, a rice line, IAC103, was selected for mutational studies with gamma radiation and ethyl methyl sulfonate mutagenesis, with the aim of developing a herbicide-resistant crop. After mutagenesis, surviving plants were exposed to glufosinate to check for herbicide resistance, which was examined up to the second generation. A detailed RAPD analysis was made of the resistant plants. Eighty Operon technology primers were tested and 10 were selected for a detailed study of RAPD markers that could tag herbicide resistance genes. Resistant and susceptible lines produced variation in the RAPD patterns and certain bands were found only in certain lines. These results suggest genetic ligation that will be confirmed through a genetic segregation study.
Asunto(s)
Resistencia a Medicamentos/genética , Mutagénesis/genética , Oryza/genética , Aminobutiratos/farmacología , Metanosulfonato de Etilo/farmacología , Rayos gamma , Marcadores Genéticos , Herbicidas/farmacología , Mutagénesis/efectos de los fármacos , Mutágenos/farmacología , Oryza/efectos de los fármacos , Oryza/crecimiento & desarrollo , Técnica del ADN Polimorfo Amplificado Aleatorio , Selección GenéticaRESUMEN
The production of phospholipase and proteinase exoenzymes was evaluated in seventy nine samples of Candida isolated from the oral cavity of patients with oral lesions characteristic of candidosis and from individuals presenting a clinically normal mouth, attended at the University of Dentistry of Ribeirão Preto USP. Among the strains of C. albicans isolated from oral lesions, the phospholipase and proteinase were detected in 83.3% and 66.7%, respectively. C. tropicalis and C. parapsilosis produced only proteinase. Regarding the isolated strains from niches without lesions, out of a total of 32 C. albicans, 71.9% presented phospholipase and 68.7% proteinase. C. tropicalis only presented the enzyme proteinase, C. glabrata, C. krusei, C. guilliermondii and Candida spp did not present any of the exoenzymes. Among the samples of C. albicans from both groups, the enzymotype 22 (positive phospholipase and proteinase weakly positive), was prevalent. Different enzymotypes of the same species were detected in samples collected from the same patient.
Asunto(s)
Candida albicans/clasificación , Candida albicans/enzimología , Endopeptidasas/biosíntesis , Boca/microbiología , Fosfolipasas/biosíntesis , Adulto , HumanosRESUMEN
The efficacy of a paste for complete dentures was analyzed in terms of denture plaque/biofilm removal and antimicrobial action against specific microorganisms by determination of colony forming units of mutans group streptococci and yeast from 120 full denture wearers with a healthy palatine mucosa. The patients were given a questionnaire to evaluate the experimental product in terms of important characteristics. The paste was widely accepted by the patients, and effective in denture plaque removal and antimicrobial action. The species of yeasts most frequently isolated were C. albicans, C. tropicalis and C. glabrata. We conclude that it is possible for complete denture wearers to keep their dentures clean with the regular use of a paste-like hygienic product.
Asunto(s)
Antiinfecciosos/uso terapéutico , Biopelículas/efectos de los fármacos , Placa Dental/microbiología , Dentadura Completa Superior/microbiología , Streptococcus mutans/efectos de los fármacos , Pastas de Dientes/uso terapéutico , Anciano , Anciano de 80 o más Años , Antibacterianos , Candida/clasificación , Candida/efectos de los fármacos , Candida/crecimiento & desarrollo , Candida albicans/efectos de los fármacos , Candida albicans/crecimiento & desarrollo , Recuento de Colonia Microbiana , Comportamiento del Consumidor , Placa Dental/prevención & control , Estudios de Seguimiento , Humanos , Persona de Mediana Edad , Mucosa Bucal/microbiología , Estadísticas no Paramétricas , Streptococcus mutans/crecimiento & desarrollo , Encuestas y CuestionariosRESUMEN
Existem evidências das vantagens e influência na sobrevida dos pacientes revascularizados com a artéria torácica interna (ATI). Entretanto, outros enxertos foram introduzidos. O objetivo do presente estudo é mostrar as vantagens da revascularizaçäo completa do miocárdio com enxertos arteriais compostos e análise dos resultados a curto e médio prazos. Entre junho de 1992 e dezembro de 1997, 50 pacientes foram submetidos à cirurgia de revascularizaçäo completa do miocárdio com enxertos arteriais compostos. A idade variou de 41 a 88 anos, com média de 56 anos. Todos os pacientes foram avaliados segundo o protocolo de exames clínicos, ECG, raios X de tórax, ecocardiograma e cateterismo cardíaco. Apresentaram fatores de risco para doença arterial coronária: HAS em 28 por cento diabete melito em 2 por cento, tabagismo em 38 por cento e dislipidemia em 50 por cento. Neste grupo 44 por cento tinham IAM prévio e 20 por cento angina instável. Todos foram classificados segundo o grau de angina da Canadian Cardiology Society (CCS) e classe funcional segundo a New York Heart Association (NYHA). As lesöes coronárias eram triarteriais em 46 por cento, biarteriais em 38 por cento, uniarteriais em 16 por cento e lesäo de tronco de coronária esquerda em 16 por cento. Em 80 por cento dos pacientes foi utilizada a circulaçäo extracorpórea (CEC), hipotermia moderada e proteçäo intermitente do miocárdio. Em média foram 2,9 pontes por paciente. Em todos os casos a ATI foi utilizada com prolongamento de veia safena, em 6 por cento veia safena em "Y", em 24 por cento artéria radial em "Y" em 40 por cento, e em 80 por cento dos casos com "Y" da própria ATI. O acompanhamento pós-operatório incluiu exame clínico, prova de esforço e/ou cintilografia miocárdica. Em 14 por cento foi realizada a coronariografia. As complicaçöes mais freqüentes no pós-operatório foram atelectasia pulmonar em 16 por cento, mediastinite em 4 por cento, IAM transoperatório em 4 por cento, SARA 4 por cento, AVCI 2 por cento, sendo a morbidade maior no grupo de pacientes do sexo feminino e com diabete melito. A mortalidade hospitalar foi de 2 por cento. Apenas um paciente apresenta quadro de angina grau II da CCS. Podemos concluir com este estudo que a cirurgia de revascularizaçäo do miocárdio com o uso de enxertos compostos pode ser realizada com baixa mortalidade, permite maior número de anastomoses e proporciona excelente evoluçäo pós-operatória a curto e médio prazos.
Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Arterias Torácicas/cirugía , Arteria Radial/cirugía , Revascularización Miocárdica/métodos , Anciano de 80 o más AñosRESUMEN
Vaginal candidiasis continues to be a common cause of vaginal discharge, pruritus and other local complaints in women worldwide. Although numerous antimycotic agents are available for the treatment of yeast vaginitis there is little comparative data on the in vitro activity of these drugs. The objectives of this study were to isolate and identify the Candida species in the vagina and anus of patients treated in a gynaecology clinic, as well as determine the susceptibility to azolic compounds measured by the E-test method. Vaginal and rectal swabs were collected from 80 adult non-pregnant patients, seen at a gynaecological clinic, aged 18-59 years, with sexual activity, with and without vaginitis. The swabs were processed by methods routinely used for the detection of pathogenic yeasts. The susceptibility of the isolates to fluconazole, ketoconazole and itraconazole, was measured by the agar diffusion method (E-test), using RPM1 1,640 medium with 2% glucose and phosphate buffer. Candida species (33) strains were isolated from 17 patients at similar proportions from both anatomical sites, and 12 patients harboured 24 strains of C. albicans in the vaginal and rectal tracts. Twenty one percent of the strains of C. albicans were resistant to ketoconazole, 54% were resistant to itraconazole and 0% were resistant to fluconazole. The sensitivity of strains isolated from the two sites were similar, indicating that these are strains of the same phenotype.
Asunto(s)
Antifúngicos/farmacología , Candida/efectos de los fármacos , Candidiasis Vulvovaginal/microbiología , Vagina/microbiología , Adolescente , Adulto , Canal Anal/microbiología , Candida/clasificación , Candida/aislamiento & purificación , Farmacorresistencia Microbiana , Femenino , Humanos , Pruebas de Sensibilidad Microbiana/métodos , Persona de Mediana EdadRESUMEN
Se estudiaron 108 personas provenientes de 22 familias compuestas de 3 a 8 miembros cada una, para la búsqueda de morfotipos de candida albicans de la cavidad bucal con o sin piezas dentarias. Por la técnica de phongpaichit et. al., morfotipos idénticos o diferentes fueron detectados en 7 grupos familiares. En 10 familias, c. albicans fue aislada de la familia y de protesis dentarias removibles de un mismo individuo. La franja discontinua, considerada como probable indicador de virulencia fue detectada en 9 (16,7 porciento) de las cepas de c. albicans de personas con dentición completa y de saliva de usuarios y no usuarios de prótesis. La franja continua fue verificada en 5 (83,3 porciento) de las cepas de c. albicans aisladas de un mismo sitio. El morfotipo es simple, fácil de ejecutar, puede servir para detectar infección cruzada y como un posible indicador de riesgo de candidosis. C. albicans fue la especie prevalente (86,4 porciento), detectándose a lo menos en 2 miembros de 14 familias
Asunto(s)
Humanos , Candida albicans/aislamiento & purificación , Mucosa Bucal/parasitología , Prótesis Dental/parasitología , Candida albicans/clasificaciónRESUMEN
Se estudiaron en agar sabouraud dextrosado, muestras obtenidas del conducto auditivo externo de 80 perros y 22 gatos aparentemente sanos. El examen micológico directo y las características morfofisiológicas y bioquímicas, permitieron determinar la presencia de malassezia pachydermatis en el 61,3 porciento de las muestras provenientes de perros y en el 13,6 porciento de los gatos. La susceptibilidad de esta levadura se determinó en medios sólidos, por el método de dilución in vitro frente a soluciones de yodo, timerosal y violeta de genciana. Todas las cepas fueron inhibidas en las siguientes concentraciones: yodo 1,0 g/l; timerosal 0,0125 g/l y violeta de genciana a 0,0078 g/l, lo que representa su dilución comercial diluída 641 veces
Asunto(s)
Animales , Gatos , Perros , Violeta de Genciana/uso terapéutico , Yodo/uso terapéutico , Malassezia/efectos de los fármacos , Oído Externo/parasitología , Timerosal/uso terapéutico , Brasil , Gatos/parasitología , Perros/parasitología , Malassezia/aislamiento & purificaciónRESUMEN
The authors studied the killer effect of nine standard strains of yeasts on 146 samples of Candida albicans isolated from the following clinical specimens: oral mucosa, feces, bronchial wash, sputum, vaginal secretion, urine, skin lesion, nail lesion and blood. Using this system it was possible to differentiate 23 biotypes of Candida albicans. The biotypes 211, 111 and 811 were most frequently isolated. Most of the samples of C. albicans (98.6%) were sensitive to at least one or more of the nine killer strains. Using the killer system it was possible to show that two patients harbored the same killer biotypes, 111 and 211, respectively, in different clinical specimens and another patient harbored the same biotype (211) in bloodcultures effected in different ocasions. The utilization of the killer system to differentiate types among species of pathogenic yeasts can be a useful method to establish the eventual source of infection, and it is a valuable tool to control and watch for nosocomial infections caused by yeasts.
Asunto(s)
Candida albicans/clasificación , Proteínas Fúngicas/clasificación , Micotoxinas/clasificación , Candida albicans/aislamiento & purificación , Humanos , Factores Asesinos de Levadura , Técnicas de Tipificación MicológicaRESUMEN
De março de 1984 a outubro de 1991 foram realizadas no Distrito Federal 320 angioplastias coronárias e 31 valvoplastias pulmonares. Dos pacientes submetidos a angioplastia coronária houve predominância do sexo masculino e da insuficiência coronária instável, como quadro clínico de apresentaçäo. Noventa e dois por cento dos procedimentos foram por via braquial utilizando cateteres do tipo Stertzer e o ramo descendente anterior foi o mais comumente acometido. Foram abordadas 357 lesSes em 320 pacientes com 87,8 por cento de sucesso primário e 11,1 por cento de reestenose nos casos estudados ao fim de seis meses. Dos 39 pacientes em que näo se obteve sucesso inicial a maioria (41 por cento) se submeteu a revascularizaçäo cirúrgica de emergência com sucesso e houve 4 óbitos, todos por dissecçäo de tronco da coronária esquerda (1,2 por cento do total). Obtivemos sucesso em 90,3 por cento dos casos de estenose valvar pulmonar. Todos os pacientes näo dilatados foram encaminhados a tratamento cirúrgico sendo que um deles, portador de insuficiência cardíaca refratária, faleceu no pós-operatório imediato. O acompanhamento dos pacientes mostrou a persistência do resultado inicial em todos eles. Concluimos que os dois procedimentos se firmaram no arsenal terapêutico cardiológico como alternativas para o tratamento cirúrgico, em casos selecionados